Title: Optimization of triploid induction for the oyster Crassostrea virginica (Gmelin)
Abstract: Standard techniques developed for producing triploid oysters (Crassostrea gigas) in the northwestern United States cause > 90% mortality of Crassostrea virginica, which is unacceptably high considering the scale of most east coast hatcheries. We examined the effect of cytochalasin B (CB) concentration, timing of treatment after fertilization, and duration of treatment on survival and triploid induction of C. virginica embryos. Survival to D-stage was inversely related to CB concentration and length of treatment. Induction of triploidy was CB-dose dependent. Optimal treatment of 0.25 mg/l CB for 10–15 min beginning when half the embryos were observed to be undergoing meiosis-I yielded mean survival to D-stage of 84% of control values with a mean of 96% triploid production.
Publication Year: 1992
Publication Date: 1992-08-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 27
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