Title: Photodynamic inactivation of the models Mycobacterium phlei and Mycobacterium smegmatis in vitro
Abstract: Photodynamic inactivation (PDI) of bacterial strains presents an attractive potential alternative to antibiotic therapies. Success is dependent on the effective accumulation in bacterial cells of photochemical substances called photosensitizers, which are usually porphyrins or their derivatives. The kinetics of porphyrin synthesis after treatment with the precursor ALA and the accumulation of the Chlorin e6 and the following illumination were studied. The goal was to estimate effectivity of the destructive power of these PS <i>in vitro</i> in respect of the physiological states of Mycobacteria. So the present results examine the cell destruction by PDI using ALA-induced Porphyrins and Chlorin e6 accumulated in <i>Mycobacterium phlei</i> and <i>Mycobacterium smegmatis</i>, which serve as models for the important pathogens <i>Mycobacterium tuberculosis</i>, <i>Mycobacterium leprae</i> and <i>Mycobacterium bovis</i>. We could show that both Mycobacterium after ALA and Chlorin e6 application were killed by illumination with light of about 662 nm. A reduction of about 97% could be reached by using a lightdose of 70 mW/cm<sup>2</sup>.
Publication Year: 2009
Publication Date: 2009-07-02
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 1
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