Title: Improved Aptazyme Design and In Vivo Screening Enable Riboswitching in Bacteria
Abstract: Angewandte Chemie International EditionVolume 47, Issue 14 p. 2604-2607 Communication Improved Aptazyme Design and In Vivo Screening Enable Riboswitching in Bacteria† Markus Wieland, Markus Wieland Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140 http://www.uni-konstanz.de/FuF/chemie/jhartigSearch for more papers by this authorJörg S. Hartig Prof. Dr., Jörg S. Hartig Prof. Dr. [email protected] Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140 http://www.uni-konstanz.de/FuF/chemie/jhartigSearch for more papers by this author Markus Wieland, Markus Wieland Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140 http://www.uni-konstanz.de/FuF/chemie/jhartigSearch for more papers by this authorJörg S. Hartig Prof. Dr., Jörg S. Hartig Prof. Dr. [email protected] Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140 http://www.uni-konstanz.de/FuF/chemie/jhartigSearch for more papers by this author First published: 18 March 2008 https://doi.org/10.1002/anie.200703700Citations: 149 † J.S.H. gratefully acknowledges the VolkswagenStiftung for funding a Lichtenberg Professorship. We thank Astrid Joachimi for excellent technical assistance. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Switch-hitter: A new strategy to control gene expression relies on a ligand-dependent, ribozyme-based RNA switch. The key feature of this setup is that the ribozyme liberates the ribosome binding site upon cleavage of the message. The design made it possible to screen in vivo for an inducible aptazyme by attaching an aptamer to stem III of the ribozyme, thus preserving naturally occurring stem I/stem II contacts. Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z703700_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1W. C. Winkler, R. R. Breaker, Annu. Rev. Microbiol. 2005, 59, 487. 2W. C. Winkler, A. Nahvi, A. Roth, J. A. Collins, R. R. Breaker, Nature 2004, 428, 281. 3G. A. Soukup, R. R. Breaker, Proc. Natl. Acad. Sci. USA 1999, 96, 3584. 4J. S. Hartig, S. H. Najafi-Shoushtari, I. Grune, A. Yan, A. D. Ellington, M. Famulok, Nat. Biotechnol. 2002, 20, 717. 5A. Khvorova, A. Lescoute, E. Westhof, S. D. Jayasena, Nat. Struct. Biol. 2003, 10, 708. 6M. Martick, W. G. Scott, Cell 2006, 126, 309. 7M. D. Canny, F. M. Jucker, E. Kellogg, A. Khvorova, S. D. Jayasena, A. Pardi, J. Am. Chem. Soc. 2004, 126, 10848. 8L. Yen, J. Svendsen, J. S. Lee, J. T. Gray, M. Magnier, T. Baba, R. J. D'Amato, R. C. Mulligan, Nature 2004, 431, 471. 9L. Yen, M. Magnier, R. Weissleder, B. R. Stockwell, R. C. Mulligan, RNA 2006, 12, 797. 10G. Werstuck, M. R. Green, Science 1998, 282, 296. 11R. D. Jenison, S. C. Gill, A. Pardi, B. Polisky, Science 1994, 263, 1425. 12B. Suess, B. Fink, C. Berens, R. Stentz, W. Hillen, Nucleic Acids Res. 2004, 32, 1610. 13S. K. Desai, J. P. Gallivan, J. Am. Chem. Soc. 2004, 126, 13247. 14S. A. Lynch, S. K. Desai, H. K. Sajja, J. P. Gallivan, Chem. Biol. 2007, 14, 173. 15S. Topp, J. P. Gallivan, J. Am. Chem. Soc. 2007, 129, 6807. 16P. J. Schlax, D. J. Worhunsky, Mol. Microbiol. 2003, 48, 1157. 17W. Winkler, A. Nahvi, R. R. Breaker, Nature 2002, 419, 952. 18J. A. Collins, I. Irnov, S. Baker, W. C. Winkler, Genes Dev. 2007, 21, 3356. 19M. N. Win, C. D. Smolke, Proc. Natl. Acad. Sci. USA 2007, 104, 14283. 20K. Salehi-Ashtiani, J. W. Szostak, Nature 2001, 414, 82. 21J. Darnell, H. F. Lodish, D. Baltimore, Molecular Cell Biology, Freeman, New York, 1986. 22A. Romani, A. Scarpa, Arch. Biochem. Biophys. 1992, 298, 1. Citing Literature Volume47, Issue14March 25, 2008Pages 2604-2607 ReferencesRelatedInformation
Publication Year: 2008
Publication Date: 2008-03-18
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 171
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