Title: Effect of methanol on the frequency of respiration-deficient mutation induced by ethidium bromide in yeast under growing and non-growing conditions.
Abstract:The effect of methanol on the frequency of cytoplasmic respiration-deficient (RD) mutation induced by ethidium bromide was investigated in Saccharomyces cerevisiae. When growing cells were treated wit...The effect of methanol on the frequency of cytoplasmic respiration-deficient (RD) mutation induced by ethidium bromide was investigated in Saccharomyces cerevisiae. When growing cells were treated with ethidium bromide at concentrations higher than 1.0μg/ml, 95 to 100% of surviving cells were RD mutants as judged by the tetrazolium overlay method. The RD mutants induced by 1.0μg/ml ethidium bromide were drastically decreased by addition of 6-8% methanol in both growing and non-growing conditions. Methanol reduced uptake of ethidium bromide by yeast cells, but it did not modify the inhibitory effect of ethidium bromide on in vitro yeast mitochondrial deoxyribonucleic acid synthesis.Read More