Title: β‐Glucosidases from Soybeans Hydrolyze Daidzin and Genistin
Abstract:ABSTRACT β‐Glucosidases which hydrolyze isoflavone glucosides, daidzin and genistin, were partially purified from soybean cotyledon. Three iso‐forms of β‐glucosidases (A,B,C) were separated on a CM‐Se...ABSTRACT β‐Glucosidases which hydrolyze isoflavone glucosides, daidzin and genistin, were partially purified from soybean cotyledon. Three iso‐forms of β‐glucosidases (A,B,C) were separated on a CM‐Sephadex C‐50 ion exchange column. β‐Glucosidase‐B and C brought about nearly all of the hydrolyzing action on daidzin and genistin. β‐Glu‐cosidase‐B and C were further purified with gel‐filtration chromatog‐raphy, and the partially purified enzymes were characterized at pH 5.0 and 45°C, (optimum conditions). Adding the β‐glucosidase‐B and C to soymilk at 45°C, both enzymes hydrolyzed daidzin and genistin and caused an increase in aglycones daidzein and genistein.Read More
Publication Year: 1993
Publication Date: 1993-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 141
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