Title: Effect of controlled colcemid exposure on human metaphase chromosome structure
Abstract: HereditasVolume 91, Issue 1 p. 49-52 Open Access Effect of controlled colcemid exposure on human metaphase chromosome structure MOGENS RØNNE, Corresponding Author MOGENS RØNNE The Winslow Institute of Human Anatomy, University of Odense, Denmark3The Winslow Institute of Human Anatomy, University of Odense, Campusvej 55, DK-5230 Odense M, DenmarkSearch for more papers by this authorKIRSTEN VANG NIELSEN, KIRSTEN VANG NIELSEN The Winslow Institute of Human Anatomy, University of Odense, DenmarkSearch for more papers by this authorMOGENS ERLANDSEN, MOGENS ERLANDSEN The Institute of Mathematics, University of Odense, DenmarkSearch for more papers by this author MOGENS RØNNE, Corresponding Author MOGENS RØNNE The Winslow Institute of Human Anatomy, University of Odense, Denmark3The Winslow Institute of Human Anatomy, University of Odense, Campusvej 55, DK-5230 Odense M, DenmarkSearch for more papers by this authorKIRSTEN VANG NIELSEN, KIRSTEN VANG NIELSEN The Winslow Institute of Human Anatomy, University of Odense, DenmarkSearch for more papers by this authorMOGENS ERLANDSEN, MOGENS ERLANDSEN The Institute of Mathematics, University of Odense, DenmarkSearch for more papers by this author First published: September 1979 https://doi.org/10.1111/j.1601-5223.1979.tb01640.xCitations: 8AboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract When human lymphoid cells are exposed to colcemid overnight at 4°C and subsequently incubated at 37.5°–38°C, variation in incubation time offers a simple method to produce chromosome slides with a high frequency of either prophase, prometaphase, or metaphase chromosomes. Literature cited Andersen, O. And Rønne, M. 1978. Effect of histidinol and parafluorophenylalanine on metaphase chromosome structure in human lymphoid cells. Hereditas 88: 197– 201. Brown, B. W. Jr. And Hollander, M. 1977. Analysis of the two-sample location problem using sample means. – In Statistics. A Biomedical Introduction, John Wiley and Sons, New York, p. 109– 129. Kosztolanyi, G. 1978. The effect of SH-SS transition on chromosome morphology and C banding pattern. Clin. Genet. 14: 298– 299. Rønne, M. 1977. Induction of uncoiled chromosomes with RNA'se. Hereditas 86: 245– 250. Rønne, M. Human cells in suspension. I. Human lymphoid and granulopoietic cells in primary and secondary cultures. The effects of in vitro induction and prolonged methanol acetic acid fixation on metaphase chromosome structure. Cytobios: in press. Rønnk, M. And Sandermann, J. 1977. Simple methods to induce bands in human chromosomes. Hereditas 86: 151– 154. Rønne, M. And Andersen, O. 1978. In vitro induction of uncoiled chromosomes with inhibitors of RNA and protein synthesis. Cytobios 21: 7– 24. Rønne, M., Andersen, O. And Erlandsen, M. 1979. Effect of colcemid exposure and methanol acetic acid fixation on human metaphase chromosome structure. Hereditas 90: 195– 201. Sumner, A. T. 1976. Banding as a level of chromosome organization. – In Current Chromosome Research. (Eds. K. Jones and P. E. Brandham), Elsevier, North-Holland Biochemical Press, Amsterdam, p. 17– 22. Tobey, R. A., Anderson, E. C. And Petersen, D. F. 1967. Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J. Cell. Physiol. 70: 63– 68. Wang, H. C., Fedoroff, S. 1972. Banding in human chromosomes treated with trypsin. Nature New Biol. 235: 52– 53. Yunis, J. J. And Sanchez, O. 1975. The G banded prophase chromosomes of man. Humangenetik 27: 167– 172. Yunis, J. J., Sawyer, J. R. And Ball, D. W. 1978. The characterization of high-resolution G-banded chromosomes of man. Chromosoma 67: 293– 307. Citing Literature Volume91, Issue1September 1979Pages 49-52 ReferencesRelatedInformation