Title: Molecular properties of isolated surface immunoglobulins of human chronic lymphocytic leukemia cells
Abstract: Abstract Lactoperoxidase-catalyzed radioiodination, coupled with immunological and biochemical techniques, was used to analyze the molecular properties of isolated surface immunoglobulins of chronic lymphocytic leukemia cells from eight patients. Lymphocytes from six of these patients possessed surface immunoglobulin extractable by the methodology applied. This immunoglobulin possessed μ-chains and light chains as assessed by polyacrylamide gel electrophoresis and antigenic analysis and the intact structure resembled the 7-S subunit of γM immunoglobulin. The number of molecules per cell on those cells possessing immunoglobulin ranged from approx. 20 000 to about 640 000 molecules. Incorporation of [3H]leucine into immunoglobulin produced by two leukemia cell samples indicated that these cells synthesized γM immunoglobulin but secreted very little into the medium. In one case, surface immunoglobulin contained γM and γG immunoglobulin, but only γM (7-S) and free light chains were found within the cells. This study provides direct evidence that chronic lymphocytic leukemia cells, like lymphomas, synthesize 7-S γM molecules which are expressed on the cell surface.
Publication Year: 1974
Publication Date: 1974-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 12
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