Title: A rapid and sensitive high pressure liquid chromatography assay for deoxyribonucleoside triphosphates in cell extracts
Abstract: An assay for simultaneous quantitation of deoxyribonucleoside triphosphates (dNTPs) in cell extracts is described. Following destruction of ribonucleotides by treatment with periodate and methylamine, dNTPs are separated by high pressure liquid chromatography and quantitated by their ultraviolet absorbance upon elution. Destruction of ribonucleotides is complete (>99.9%), the resulting mixture is suitable for direct injection without further manipulation, and recovery of dNTPs is reproducible and near quantitative. This technique offers a simple and rapid alternative to either DNA polymerase or thin-layer chromatography assays for dNTPs in cellular extracts.
Publication Year: 1979
Publication Date: 1979-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 272
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot