Publication Information

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'https://openalex.org/W4319308111', 'https://openalex.org/W946202211'], 'related_works': ['https://openalex.org/W4253123437', 'https://openalex.org/W2415751525', 'https://openalex.org/W2345641464', 'https://openalex.org/W2337255094', 'https://openalex.org/W2333731847', 'https://openalex.org/W2187294308', 'https://openalex.org/W2128772347', 'https://openalex.org/W2123422455', 'https://openalex.org/W1980132045', 'https://openalex.org/W1967127905'], 'abstract_inverted_index': {'Cytidine': [0, 232], 'monophospho-N-acetylneuraminic': [1, 233], 'acid': [2, 15, 35, 110, 234, 247, 267, 342, 467, 562, 601, 605, 967, 999, 1219, 1553, 1746, 1767, 2350, 2415, 2490, 2777, 2931, 3264, 3362, 3378, 3394, 3408, 3915, 3948, 4006, 4890, 5016], '(CMP-NeuAc)': [3, 235], 'hydroxylase,': [4, 41, 236, 273, 1596, 2127, 3341, 4708], 'which': [5, 141, 237, 373, 829, 1246, 2132, 2176, 2850, 3413, 3694, 3990, 4012, 4709], 'is': [6, 142, 205, 238, 374, 437, 468, 563, 756, 821, 930, 1207, 1223, 1266, 1272, 1445, 1555, 1586, 2682, 3410, 3414, 3493, 3528, 3674, 3695, 3983, 3991, 4093, 4104, 4205, 4456, 4703, 4754, 4965], 'the': [7, 11, 21, 38, 60, 68, 81, 108, 114, 147, 150, 161, 165, 171, 194, 208, 219, 227, 239, 243, 253, 270, 292, 300, 313, 340, 346, 379, 382, 393, 397, 403, 426, 440, 451, 459, 674, 699, 749, 819, 849, 919, 928, 1165, 1211, 1217, 1433, 1485, 1566, 1581, 1591, 1608, 1646, 1755, 1762, 1765, 1770, 1774, 1803, 1927, 1937, 1964, 2070, 2073, 2076, 2121, 2135, 2147, 2173, 2181, 2184, 2193, 2220, 2264, 2304, 2329, 2340, 2346, 2379, 2436, 2442, 2448, 2504, 2551, 2686, 2691, 2705, 2725, 2736, 2771, 2780, 2785, 2844, 2897, 2928, 2950, 3010, 3015, 3019, 3031, 3035, 3057, 3064, 3073, 3093, 3104, 3107, 3122, 3167, 3174, 3210, 3216, 3220, 3224, 3238, 3261, 3270, 3280, 3307, 3329, 3339, 3357, 3360, 3365, 3373, 3386, 3391, 3405, 3480, 3491, 3507, 3512, 3534, 3546, 3569, 3572, 3578, 3613, 3624, 3676, 3689, 3699, 3946, 3965, 4010, 4047, 4055, 4091, 4108, 4111, 4119, 4123, 4141, 4145, 4149, 4155, 4160, 4179, 4198, 4202, 4211, 4250, 4259, 4269, 4277, 4291, 4334, 4337, 4347, 4444, 4461, 4469, 4473, 4476, 4488, 4598, 4616, 4626, 4660, 4666, 4692, 4695, 4711, 4721, 4749, 4759, 4765, 4768, 4775, 4785, 4792, 4795, 4801, 4804, 4810, 4828, 4835, 4839, 4976, 4979, 4991, 5003, 5006, 5014, 5019, 5027, 5045, 5053, 5088], 'key': [8, 240], 'enzyme': [9, 61, 116, 129, 148, 162, 195, 241, 293, 348, 361, 380, 394, 427, 929, 1649, 3016, 3481, 3492, 3982, 4051, 4157], 'for': [10, 89, 113, 125, 242, 321, 345, 357, 568, 621, 653, 752, 980, 1012, 1568, 1885, 1889, 1894, 2064, 2393, 2418, 2447, 2630, 2646, 2710, 2719, 2798, 2803, 2823, 2915, 2927, 3018, 3092, 3269, 3310, 3550, 3657, 3998, 4137, 4169, 4720, 5052, 5070, 5086, 5094], 'synthesis': [12, 244, 4750], 'of': [13, 24, 37, 59, 67, 75, 80, 92, 101, 146, 153, 167, 170, 189, 203, 210, 245, 256, 269, 291, 299, 307, 312, 324, 333, 378, 385, 399, 402, 421, 435, 442, 537, 673, 698, 702, 754, 758, 922, 1032, 1093, 1149, 1167, 1213, 1221, 1242, 1264, 1285, 1323, 1435, 1462, 1576, 1583, 1594, 1598, 1607, 1616, 1645, 1658, 1667, 1676, 1717, 1754, 1764, 1769, 1807, 1839, 1853, 1883, 2047, 2072, 2115, 2125, 2131, 2139, 2175, 2192, 2219, 2234, 2292, 2327, 2339, 2348, 2387, 2405, 2409, 2435, 2450, 2510, 2555, 2679, 2690, 2698, 2727, 2779, 2808, 2819, 2949, 2999, 3014, 3027, 3034, 3076, 3103, 3106, 3121, 3166, 3173, 3209, 3237, 3299, 3338, 3364, 3372, 3390, 3451, 3479, 3519, 3536, 3555, 3574, 3581, 3667, 3707, 3712, 3749, 3861, 3864, 3945, 3950, 3972, 3980, 4113, 4122, 4144, 4181, 4188, 4214, 4271, 4273, 4279, 4290, 4336, 4358, 4447, 4463, 4472, 4478, 4490, 4600, 4611, 4621, 4629, 4663, 4691, 4694, 4705, 4714, 4726, 4751, 4761, 4767, 4777, 4787, 4803, 4812, 4818, 4834, 4838, 4841, 4849, 4857, 4886, 4947, 4957, 4969, 4971, 4975, 4985, 4987, 4993, 5044, 5049, 5067], 'N-glycolylneuraminic': [14, 246, 604, 966, 998], '(NeuGc),': [16, 248], 'has': [17, 130, 181, 249, 362, 413, 3283, 4604], 'been': [18, 182, 250, 414, 3285, 3918, 4606], 'purified': [19, 39, 115, 251, 271, 347, 915, 1648, 1756, 1939, 2230, 2305, 3340, 3374, 4053], 'from': [20, 252, 693, 844, 918, 1936, 1980, 2263, 2482, 2674, 2813, 2847, 3404, 3586, 4054, 4950], 'cytosolic': [22, 254, 920, 2443, 3500, 4142, 5028], 'fraction': [23, 255, 921, 2444], 'mouse': [25, 56, 191, 257, 288, 423, 923, 1155, 1603, 1818, 2154, 2483, 2814, 2848, 3551, 3587, 4220], 'liver,': [26, 258, 3590], 'as': [27, 197, 259, 429, 944, 1777, 1822, 2088, 2183, 2457, 2708, 2718, 2853, 3023, 3158, 3193, 3319, 3498, 4009, 4854], 'described': [28, 260, 1620, 2709], 'in': [29, 149, 226, 261, 381, 458, 470, 535, 1036, 1154, 1210, 1253, 1287, 1292, 1492, 1558, 1602, 1611, 1655, 1720, 1724, 1779, 1844, 1984, 2225, 2271, 2384, 2411, 2518, 2580, 2621, 2637, 2704, 2790, 2834, 3160, 3276, 3297, 3381, 3385, 3397, 3415, 3504, 3558, 3563, 3612, 3623, 3688, 3716, 3722, 3753, 3859, 3888, 3920, 4046, 4083, 4126, 4197, 4219, 4251, 4281, 4285, 4346, 4468, 4475, 4495, 4615, 4625, 4643, 4652, 4665, 4699, 4791, 4814, 4960, 4996, 5026], 'our': [30, 262], 'previous': [31, 263], 'paper.': [32, 264], 'The': [33, 78, 104, 128, 265, 310, 336, 360, 619, 651, 840, 978, 1010, 1030, 1147, 1283, 1458, 1689, 1905, 1955, 2010, 2032, 2616, 2649, 2677, 2754, 2775, 2883, 2918, 3171, 3203, 3234, 3255, 3313, 3323, 3400, 3540, 3664, 3691, 3978, 4050, 4266, 4288, 4609, 4816, 5076], 'amino': [34, 94, 109, 266, 326, 341, 1745, 1766, 2117, 2776, 2930, 3263, 3267, 3326, 3345, 3361, 3377, 3393, 3407, 3947, 4005], 'sequences': [36, 111, 268, 343, 1610, 1768, 2778, 2795, 3217, 3363], 'CMP-NeuAc': [40, 211, 272, 443, 838, 916, 934, 1168, 1584, 1595, 1617, 2126, 2454, 2556, 2862, 3537, 3552, 3575, 3951, 4048, 4166, 4256, 4274, 4338, 4602, 4622, 4696, 4707, 4715, 4798, 4895, 4958, 5020, 5023], 'and': [42, 72, 117, 119, 164, 229, 274, 304, 349, 351, 396, 461, 492, 534, 571, 580, 603, 678, 837, 847, 925, 935, 1090, 1151, 1156, 1244, 1258, 1276, 1320, 1464, 1479, 1484, 1570, 1572, 1605, 1652, 1732, 1773, 1830, 1836, 1868, 1892, 1915, 1947, 1973, 1975, 2025, 2045, 2142, 2164, 2229, 2288, 2334, 2396, 2422, 2441, 2453, 2497, 2525, 2592, 2611, 2625, 2633, 2641, 2653, 2744, 2751, 2784, 2793, 2826, 2830, 2892, 2905, 2911, 2933, 2955, 2975, 2994, 3021, 3080, 3087, 3099, 3119, 3138, 3155, 3163, 3182, 3198, 3206, 3215, 3227, 3248, 3252, 3260, 3295, 3342, 3369, 3592, 3619, 3650, 3669, 3682, 3726, 3737, 3858, 3959, 3968, 3975, 4101, 4134, 4191, 4258, 4276, 4363, 4405, 4723, 4771, 4806, 4844, 4884, 4888, 4990, 4998, 5005, 5011, 5018, 5041, 5055, 5058, 5064, 5074, 5090], 'peptides': [43, 275, 1772, 2783, 2824, 3368, 4136], 'obtained': [44, 63, 112, 124, 276, 295, 344, 356, 2262, 4125], 'by': [45, 64, 277, 296, 684, 824, 1429, 1441, 1562, 1684, 1695, 1811, 1880, 1910, 1968, 2066, 2092, 2231, 2309, 2336, 2426, 3113, 3151, 3188, 3421, 4110, 4333, 4824, 4954], 'lysylendopeptidase': [46, 278, 1677], 'digestion,': [47, 279], 'were': [48, 224, 280, 456, 1693, 1730, 1782, 1908, 1931, 1957, 1966, 1978, 2041, 2049, 2060, 2080, 2109, 2149, 2157, 2269, 2301, 2331, 2382, 2400, 2424, 2439, 2651, 2767, 2796, 2913, 3090, 3201, 3213, 3290, 3347, 3383, 3621, 3655, 3740, 5009, 5030], 'used': [49, 281, 567, 609, 641, 2446, 2797, 2852], 'to': [50, 157, 207, 282, 389, 439, 539, 933, 1034, 1161, 1249, 1438, 1495, 1734, 1866, 1933, 2062, 2179, 2345, 2353, 2516, 2528, 2684, 2731, 2957, 2973, 2988, 3008, 3136, 3530, 3533, 3922, 3955, 3960, 3986, 4152, 4293, 4351, 4635, 4893, 4903, 4952, 5013, 5035, 5080], 'synthesize': [51, 283], 'specific': [52, 284, 932, 2086, 2940, 4892], 'oligonucleotide': [53, 285, 1792, 2039, 2801, 2901, 3957], 'primers.': [54, 286, 2089], 'A': [55, 287, 1518, 1805, 1995, 2217, 2245, 2537, 2665, 3146, 3449, 3461, 4646], 'cDNA': [57, 76, 155, 196, 289, 308, 387, 428, 1592, 1808, 2156, 2549, 2562, 2816, 2845, 3175, 3186, 3240, 3514, 3548, 3557, 3602, 3700, 3962], 'clone': [58, 82, 290, 314], 'was': [62, 123, 294, 355, 691, 1159, 1427, 1619, 1650, 1681, 1710, 1748, 1809, 1826, 1877, 2017, 2177, 2187, 2223, 2343, 2445, 2485, 2514, 2544, 2575, 2618, 2672, 2702, 2715, 2729, 2738, 2756, 2811, 2851, 2886, 2921, 2942, 2953, 3003, 3067, 3111, 3149, 3169, 3176, 3316, 3594, 3605, 3609, 3751, 4043, 4052, 4217, 4283, 4331, 4493, 4649, 4668], 'a': [65, 90, 97, 120, 132, 137, 154, 198, 297, 322, 329, 352, 364, 369, 386, 430, 564, 945, 1094, 1490, 1698, 1713, 1823, 1845, 1864, 1898, 1941, 2014, 2052, 2112, 2167, 2226, 2429, 2519, 2529, 2547, 2565, 2656, 2699, 2759, 2806, 2825, 2854, 2958, 2971, 2984, 2997, 3109, 3134, 3164, 3185, 3194, 3207, 3243, 3485, 3499, 3523, 3564, 3600, 3705, 3717, 4003, 4015, 4094, 4099, 4170, 4612, 4653, 4855], 'combination': [66, 298], 'polymerase': [69, 301, 972, 1004, 1842, 2029], 'chain': [70, 302, 616, 648, 973, 1005], 'reaction': [71, 303, 974, 1006, 1846, 2012, 2951], 'rapid': [73, 305], 'amplification': [74, 306, 3102], 'ends.': [77, 309], 'sequence': [79, 106, 135, 172, 180, 311, 338, 367, 404, 412, 1801, 2145, 2174, 2991, 3236, 3257, 3265, 3395, 3409, 3457, 3949, 4007, 4121, 4614], 'contained': [83, 315, 1794, 3229, 3515], 'an': [84, 316, 825, 1451, 1738, 1785, 1796, 2488, 2947, 2989, 3060, 3230, 3320, 3516], 'open': [85, 317, 3245, 3271, 3387], 'reading': [86, 318, 3246, 3272, 3388], 'frame': [87, 319, 3273, 3389], 'coding': [88, 320, 2123, 2140, 2553, 3012, 3232], 'protein': [91, 323, 1735, 1742, 2451, 3967, 4096], '577': [93, 325], 'acids': [95, 327, 570, 1241, 1487, 2399, 3327, 3346, 4446, 4949], 'with': [96, 144, 173, 193, 218, 328, 376, 405, 425, 450, 1481, 1664, 1712, 1784, 1814, 1917, 1923, 1940, 1963, 1971, 2013, 2019, 2051, 2111, 2153, 2159, 2280, 2303, 2402, 2428, 2546, 2655, 2670, 2740, 2758, 2817, 2843, 2983, 3030, 3069, 3116, 3153, 3179, 3191, 3418, 3511, 3522, 3599, 3646, 3993, 4159, 4173, 4784], 'predicted': [98, 330], 'molecular': [99, 331, 3032, 3401], 'mass': [100, 332, 3033, 3402], '66': [102, 334, 3411], 'kDa.': [103, 335], 'deduced': [105, 337, 3262, 3392, 3406], 'included': [107, 339, 3223], 'peptides,': [118, 350], 'complete': [121, 353], 'match': [122, 354], '159': [126, 358, 3376], 'residues.': [127, 359], 'neither': [131, 363], 'signal': [133, 365, 4135], 'peptide': [134, 366, 1691], 'nor': [136, 368], 'membrane': [138, 370, 465, 2532, 4138], 'spanning': [139, 371], 'domain,': [140, 372], 'consistent': [143, 375, 3992], 'localization': [145, 377], 'cytosol.': [151, 383], 'Transfection': [152, 384], 'construct': [156, 388, 2218], 'COS-1': [158, 390, 2260, 2293, 3508, 3559], 'cells': [159, 391, 538, 1290, 2261, 2294, 2330, 2342, 2381, 2438, 3509, 3560, 4962], 'increased': [160, 392, 3517], 'activity': [163, 395, 2456], 'amount': [166, 398, 2678, 3518], 'NeuGc.': [168, 400, 1260, 1282], 'Comparison': [169, 401], 'GenBank': [174, 406, 3452], 'data': [175, 407, 2048, 3453, 3743, 4902], 'indicated': [176, 200, 408, 432, 747, 3218, 3242], 'that': [177, 201, 409, 433, 689, 748, 927, 1447, 1476, 1551, 2724, 3219, 3419, 3490, 3545, 3571, 3607, 3963, 4017, 4090, 4102], 'no': [178, 410, 2939, 3455, 4901], 'similar': [179, 411, 1609, 3456, 3985], 'reported': [183, 415, 2459, 3458, 3606], 'so': [184, 416, 3459], 'far.': [185, 417, 3460], 'Northern': [186, 418, 2711, 2720], 'blot': [187, 216, 419, 448, 2617, 2712, 2721, 2737], 'analysis': [188, 217, 420, 449, 1597, 2046, 2722, 5017], 'various': [190, 422, 1321], 'tissues': [192, 424, 1092, 1158, 1278, 1326, 2484, 3659, 3679, 3757], 'probe': [199, 431, 2539, 2701], 'expression': [202, 434, 1601, 2194, 3573, 3580, 3748, 4278, 4462, 4489, 4628, 4662, 4693, 4725, 4811, 4817, 4956, 4995], 'NeuGc': [204, 436, 690, 755, 1033, 1150, 1206, 1265, 1286, 1436, 1448, 1465, 1480, 1577, 2423, 3608, 3620, 3750, 4280, 4464, 4813, 4851, 4953, 4994], 'related': [206, 438, 4647], 'level': [209, 441, 1166, 4270, 4760], 'hydroxylase': [212, 444, 841, 917, 1169, 1585, 1618, 2455, 2557, 3538, 3576, 3952, 4092, 4124, 4167, 4257, 4275, 4339, 4474, 4603, 4623, 4638, 4697, 4799, 4829, 4959, 4980, 5021, 5024], 'mRNA.': [213, 445], 'On': [214, 446, 2936, 4794], 'Southern': [215, 447], 'same': [220, 452, 2182, 2687, 2706, 3677], 'probe,': [221, 453], 'cross-hybridizing': [222, 454, 3731, 4613], 'bands': [223, 455, 2650, 2766, 3654, 3673, 3684], 'detected': [225, 457, 3335, 3611, 3656, 3687, 3752, 3919, 4494, 4642, 4651], 'human': [228, 460, 1277, 1288, 1324, 2560, 3725, 3755, 4601, 4617], 'fish': [230, 462, 3727, 3865, 4654, 4701], 'genomes.': [231, 463], 'Cell': [464, 582], 'sialic': [466, 1218, 1552, 2349, 2398, 4019, 4445, 4948, 5015], 'involved': [469, 4196], 'cell-cell': [471], '(1Landmesser': [472], 'L.': [473, 475, 628, 660, 760, 797, 987, 1019, 4366, 4368, 4927], 'Dahm': [474, 4367], 'Tang': [476, 4369], 'J.C.': [477, 4023, 4370, 4911], 'Rutishauser': [478, 4371], 'U.': [479, 2206, 4372], 'Neuron.': [480, 4373], '1990;': [481, 809, 862, 1082, 1419, 3850, 4374, 4590], '4:': [482, 4375], '655-667Abstract': [483, 4376], 'Full': [484, 788, 960, 1125, 1200, 1635, 3050, 3443, 3880, 4030, 4032, 4070, 4377, 4683, 4918], 'Text': [485, 789, 961, 1126, 1201, 1636, 3051, 3444, 3881, 4031, 4033, 4071, 4378, 4684, 4919], 'PDF': [486, 790, 962, 1127, 1202, 1637, 3052, 3445, 3882, 4034, 4072, 4379, 4685, 4920], 'PubMed': [487, 513, 531, 556, 595, 634, 666, 719, 738, 769, 791, 812, 865, 888, 908, 963, 993, 1025, 1060, 1085, 1110, 1128, 1142, 1185, 1203, 1235, 1315, 1361, 1422, 1513, 1638, 2104, 2212, 2373, 2475, 2878, 3053, 3446, 3474, 3640, 3792, 3853, 3883, 3909, 3939, 4035, 4073, 4311, 4326, 4380, 4400, 4419, 4438, 4532, 4593, 4686, 4743, 4879, 4921, 4941], 'Scopus': [488, 514, 557, 596, 635, 667, 720, 739, 770, 813, 866, 889, 909, 994, 1026, 1061, 1086, 1111, 1143, 1186, 1236, 1316, 1362, 1423, 1514, 2105, 2213, 2374, 2476, 2879, 3475, 3641, 3793, 3854, 3910, 3940, 4036, 4239, 4312, 4327, 4381, 4401, 4420, 4439, 4533, 4594, 4744, 4880, 4942], '(286)': [489, 4382], 'Google': [490, 516, 532, 559, 598, 637, 669, 722, 741, 772, 792, 815, 868, 891, 911, 964, 996, 1028, 1063, 1088, 1113, 1129, 1145, 1188, 1204, 1238, 1318, 1344, 1364, 1385, 1402, 1425, 1516, 1546, 1639, 2107, 2215, 2376, 2478, 2881, 3054, 3447, 3477, 3643, 3775, 3795, 3816, 3833, 3856, 3884, 3912, 3942, 4038, 4074, 4241, 4314, 4329, 4383, 4403, 4422, 4441, 4515, 4535, 4556, 4573, 4596, 4687, 4746, 4882, 4922, 4944], 'Scholar)': [491, 533, 1089, 1319, 1426, 2108, 2982, 3478, 3857, 3885, 4330], 'cell-pathogen': [493], 'interactions': [494], '(2Suzuki': [495], 'Y.': [496, 498, 852, 871, 896, 949, 1099, 1173, 1228, 1297, 1328, 1369, 1371, 1504, 1506, 1624, 2199, 2361, 2463, 2866, 3039, 3432, 3629, 3759, 3800, 3802, 3871, 3896, 3902, 4059, 4228, 4300, 4319, 4393, 4499, 4540, 4542, 4674, 4731], 'Nagao': [497], 'Kato': [499, 1302, 1331, 1378, 1389, 1393, 1501, 3762, 3809, 3820, 3824, 4502, 4549, 4560, 4564], 'H.': [500, 875, 898, 1066, 1074, 1175, 1330, 1347, 1367, 1392, 1502, 2365, 2465, 2868, 3761, 3778, 3798, 3823, 4222, 4429, 4501, 4518, 4538, 4563, 4733, 4862], 'Suzuki': [501, 857, 876, 880, 901, 952, 1050, 1102, 1178, 1503, 1627, 2468, 2871, 3042, 3435, 3632, 4062, 4229, 4303, 4736, 4865], 'T.': [502, 543, 854, 856, 873, 879, 894, 900, 947, 951, 1045, 1047, 1049, 1053, 1068, 1101, 1171, 1177, 1305, 1388, 1500, 1622, 1626, 1992, 2242, 2461, 2467, 2864, 2870, 3037, 3041, 3430, 3434, 3631, 3819, 3900, 4057, 4061, 4224, 4232, 4302, 4391, 4431, 4433, 4559, 4729, 4735, 4868, 4872], 'Matsumoto': [503, 1075, 1335, 1374, 3766, 3805, 4506, 4545], 'M.': [504, 523, 525, 711, 777, 1070, 1072, 1226, 1307, 1334, 1336, 1349, 1373, 1375, 1396, 1405, 1409, 2359, 2367, 3765, 3767, 3780, 3804, 3806, 3827, 3836, 3840, 3898, 4317, 4411, 4425, 4505, 4507, 4520, 4544, 4546, 4567, 4576, 4580, 4864, 4866, 4870, 5084], 'Murayama': [505], 'J.': [506, 551, 629, 661, 727, 733, 782, 807, 859, 882, 904, 954, 988, 1020, 1054, 1104, 1119, 1181, 1194, 1338, 1542, 1629, 1988, 2095, 2098, 2238, 2471, 2874, 3044, 3437, 3465, 3468, 3634, 3769, 3874, 3903, 4024, 4064, 4233, 4305, 4394, 4414, 4509, 4677, 4739, 4873, 4912, 4933], 'Biochim.': [507, 1079, 1229, 4320], 'Biophys.': [508, 1080, 1230, 1309, 1355, 3786, 4321, 4526], 'Acta.': [509, 1081, 1231, 4322], '1987;': [510, 1341, 3772, 4512], '903:': [511], '417-424Crossref': [512], '(47)': [515], 'Scholar,': [517, 588, 723, 773, 793, 869, 892, 1064, 1114, 1130, 1189, 1345, 1365, 1386, 1403, 3776, 3796, 3817, 3834, 4315, 4423, 4516, 4536, 4557, 4574, 4923], '3Ono': [518], 'E.': [519, 1413, 1498, 3844, 4584], 'Abe': [520], 'K.': [521, 877, 1043, 1097, 1301, 1353, 1407, 1508, 2363, 3627, 3784, 3838, 3867, 3892, 4298, 4524, 4578, 4670], 'Nakazawa': [522], 'Naiki': [524, 1306, 1348, 1372, 1395, 3779, 3803, 3826, 4519, 4543, 4566], 'Infect.': [526], 'Immun.': [527], '1989;': [528, 785, 2370, 4938], '57:': [529], '907-911Crossref': [530], 'binding': [536], 'extra': [540], 'cellular': [541, 4364], 'matrix(4Kawano': [542], 'Takasaki': [544], 'S.': [545, 781, 1134, 1303, 1332, 1351, 1377, 1379, 1394, 1415, 1534, 2201, 2207, 2357, 3763, 3782, 3808, 3810, 3825, 3846, 3869, 3894, 4226, 4389, 4503, 4522, 4548, 4550, 4565, 4586, 4672], 'Tao': [546], 'T.-W.': [547], 'Kobata': [548], 'A.': [549, 590, 779, 795, 803, 858, 881, 902, 953, 1051, 1103, 1179, 1390, 1536, 1628, 2208, 2469, 2872, 3043, 3436, 3633, 3821, 4063, 4230, 4304, 4561, 4737, 4925, 4935], 'Int.': [550], 'Cancer.': [552, 1418, 3849, 4589], '1993;': [553, 905, 1182, 1543, 2472, 2875, 4236, 4435, 4740, 4876], '53:': [554], '91-96Crossref': [555], '(62)': [558], 'Scholar).': [560, 599, 670, 816, 912, 965, 997, 1029, 1146, 1205, 1239, 1517, 1547, 1640, 1875, 2009, 2216, 2259, 2323, 2377, 2479, 2882, 3055, 3145, 3448, 3644, 3913, 3943, 4242, 4442, 4688, 4747, 4945], 'Sialic': [561, 576, 3914, 4343], 'generic': [565], 'designation': [566], 'N-acylneuraminic': [569], 'their': [572, 5071], 'many': [573], 'derivatives(5Schauer': [574], 'R.': [575, 709, 729, 762, 801, 805, 1132, 1417, 1538, 2197, 3848, 4588, 4929, 4931], 'Acids:': [577], 'Chemistry,': [578], 'Metabolism': [579], 'Function:': [581], 'Biology': [583], 'Monographs.': [584], 'Springer-Verlag,': [585], 'New': [586, 1873, 2980, 3143], 'York1982Google': [587], '6Varki': [589], 'Glycobiology.': [591, 4434], '1992;': [592, 4416], '2:': [593], '25-40Crossref': [594], '(478)': [597], 'N-Acetylneuraminic': [600], '(NeuAc)': [602], '(NeuGc)1(': [606], '1The': [607, 639], 'abbreviations': [608, 620, 640, 652, 979, 1011], 'are:': [610, 642], 'NeuGcN-glycolylneuraminic': [611, 643], 'acidHPLChigh': [612, 644], 'performance': [613, 645, 969, 1001], 'liquid': [614, 646, 970, 1002], 'chromatographyPCRpolymerase': [615, 647], 'reactionbpbase': [617, 649], 'pair(s)kbkilobase(s).': [618, 650], 'gangliosides': [622, 654, 981, 1013, 4406], 'follow': [623, 655, 982, 1014], "Svennerholm's": [624, 656, 983, 1015], 'nomenclature': [625, 657, 984, 1016], 'system': [626, 658, 985, 1017, 4263], '(47Svennerholm': [627, 659, 986, 1018], 'Neurochem.': [630, 662, 989, 1021, 4415], '1963;': [631, 663, 990, 1022], '10:': [632, 664, 906, 991, 1023, 1183, 1544, 2473, 2876, 4741], '613-623Crossref': [633, 665, 992, 1024], '(1297)': [636, 668, 995, 1027], 'Scholar).)': [638], 'are': [671, 1247, 1466, 2788, 3274, 3303, 3685, 3744, 4080, 4195, 4264, 4349, 4452, 4900, 5033], 'two': [672, 2128, 2232, 2781], 'most': [675, 1214], 'abundant': [676], 'derivatives,': [677], 'both': [679, 3228, 3617, 3683, 3973], 'may': [680, 1488, 4013, 4717, 4807, 4820], 'be': [681, 1162, 1250, 1439, 1450, 2180, 2841, 3334, 3531, 3704, 4014, 4352, 4466, 4633, 4641, 4718, 4822], 'further': [682, 2916], 'modified': [683, 2273], 'O-acetylation.': [685], 'Some': [686], 'reports': [687], 'suggested': [688, 1160], 'produced': [692], 'NeuAc': [694, 940, 1035, 1257, 1463, 1478, 2421, 3618, 4951], 'through': [695, 3350], 'enzymatic': [696], 'hydroxylation': [697, 757, 820], 'N-acetyl': [700], 'residue': [701], 'free': [703, 939, 3496], 'NeuAc,': [704], 'CMP-NeuAc,': [705], 'or': [706, 941, 1455, 1798, 2036, 2137, 2558, 3353, 3710, 3890, 4097, 4115, 4184, 4852, 4896, 4963], 'glycoconjugate-linked': [707], 'NeuAc(7Schauer': [708], 'Wember': [710], "Hoppe-Seyler's": [712], 'Z.': [713], 'Physiol.': [714, 3935], 'Chem.': [715, 764, 784, 956, 1121, 1196, 1631, 3046, 3439, 3876, 4026, 4066, 4679, 4914], '1971;': [716], '352:': [717], '1282-1290Crossref': [718], '(33)': [721], '8Buscher': [724], 'H.P.': [725], 'Casals-Stenzel': [726], 'Schauer': [728, 761, 804, 1537, 4930], 'Mestres-Ventura': [730], 'P.': [731, 799], 'Eur.': [732, 806], 'Biochem.': [734, 808, 860, 883, 1055, 1105, 1308, 1354, 2369, 3635, 3785, 3904, 3934, 4234, 4306, 4395, 4525, 4874], '1977;': [735, 1358, 3789, 4529], '77:': [736], '297-310Crossref': [737], '(40)': [740], 'Scholar),': [742, 4039, 4075, 4384, 4404, 4597, 4883], 'but': [743, 1268, 1531, 2945, 3886, 4040, 4076, 4763], 'recent': [744], 'results': [745, 3541], 'have': [746, 914, 3916], 'major': [750, 1728, 2782], 'mechanism': [751], 'biosynthesis': [753], 'CMP-NeuAc(9Shaw': [759], 'Biol.': [763, 783, 955, 1120, 1195, 1630, 2100, 3045, 3438, 3470, 3875, 4025, 4065, 4678, 4913], 'Hoppe-Seyler.': [765], '1988;': [766, 1107, 1122, 1197, 3637, 3877, 4308, 4397, 4680], '369:': [767], '477-486Crossref': [768], '(126)': [771], '10Muchmore': [774], 'E.A.': [775], 'Milewski': [776], 'Varki': [778], 'Diaz': [780], '264:': [786], '20216-20223Abstract': [787], '11Lepers': [794], 'Shaw': [796, 4926], 'Schneckenburger': [798], 'Cacan': [800, 4928], 'Verbert': [802, 4934], '193:': [810], '715-723Crossref': [811], '(43)': [814, 4240], 'In': [817, 2069, 4656], 'addition,': [818], 'carried': [822, 1878], 'out': [823, 1879], 'electron': [826, 4077, 4161, 4261], 'transport': [827, 4078, 4162, 4262], 'system,': [828], 'includes': [830], 'NADH-dependent': [831], 'cytochrome': [832, 835, 845, 4174, 4189, 4215, 4842, 4845], 'b5': [833, 846, 4175, 4190, 4193, 4216, 4843, 4846], 'reductase,': [834], 'b5,': [836], 'hydroxylase.': [839, 1757, 4049], 'accepts': [842], 'electrons': [843, 4182], 'catalyzes': [848], 'terminal': [850], 'reaction(12Kozutsumi': [851], 'Kawano': [853, 872, 4223, 4867], 'Yamakawa': [855, 878, 1052, 1100, 3630, 4301, 5093], '(Tokyo).': [861, 884, 1056, 1106, 3636, 3905, 4235, 4307, 4396, 4875], '108:': [863], '704-706Crossref': [864], '(72)': [867], '13Kozutsumi': [870], 'Kawasaki': [874, 899, 950, 1176, 1625, 2466, 2869, 3040, 3433, 4060, 4231, 4734], '1991;': [885, 1139, 1312, 1399, 1510, 3830, 3906, 4570], '110:': [886, 3907], '429-435Crossref': [887], '(34)': [890], '14Kawano': [893], 'Kozutsumi': [895, 948, 1172, 1623, 2462, 2865, 3038, 3431, 4058, 4227, 4730], 'Takematsu': [897, 1174, 2464, 2867, 4732], 'Glycoconjugate': [903, 1180, 1541, 2470, 2873, 4738], '109-115Crossref': [907, 1184, 2474, 2877, 4742], '(29)': [910, 1187, 2477, 2880, 4745], 'We': [913, 4147, 5032], 'liver': [924, 1819, 2155, 2815], 'demonstrated': [926, 1428], 'highly': [931], 'does': [936, 2859], 'not': [937, 1259, 1280, 1467, 1532, 2840, 2860, 3284, 3333, 3483, 3686, 3887, 3917, 3984, 4044, 4130, 4605, 4757, 4780], 'use': [938, 3711], 'NeuAc-containing': [942], 'GM3': [943], 'substrate(15Kawano': [946], '1994;': [957, 1632, 3047, 3440, 4067], '269:': [958, 1633, 3048, 3441, 4068], '9024-9029Abstract': [959, 1634, 3049, 3442, 4069], 'high': [968, 1000, 3597], 'chromatography': [971, 1003], 'base': [975, 1007], 'pair(s)': [976, 1008], 'kilobase(s).': [977, 1009], 'ratio': [1031, 3666], 'glycoconjugates': [1037, 1475, 4282, 4296, 4345, 4492, 4989], 'varies': [1038], 'among': [1039, 1091, 2694, 3678], 'animal': [1040, 1613], 'species': [1041, 2351], '(16Nakamura': [1042], 'Ariga': [1044], 'Yahagi': [1046], 'Miyatake': [1048], '1983;': [1057], '94:': [1058], '1359-1365Crossref': [1059], '(45)': [1062], '17Kitagaki-Ogawa': [1065], 'Yatohgo': [1067], 'Izumi': [1069], 'Hayashi': [1071], 'Kashiwagi': [1073], 'I.': [1076, 4427, 4860], 'Seno': [1077], 'N.': [1078, 1295], '1033:': [1083], '49-56Crossref': [1084], '(52)': [1087], 'single': [1095, 3244], 'species(18Nakamura': [1096], 'Hashimoto': [1098, 1406, 3628, 3837, 4299, 4577], '103:': [1108, 3638, 4309], '201-208Crossref': [1109, 3639, 4310], '(54)': [1112, 3642, 4313], '19Bouhours': [1115, 1190], 'D.': [1116, 1191, 1299], 'Bouhours': [1117, 1192], 'J.F.': [1118, 1193], '263:': [1123, 1198, 3878, 4681], '15540-15545Abstract': [1124, 1199], '20Schauer': [1131], 'Stoll': [1133], 'Reuter': [1135], 'G.': [1136], 'Carbohydr.': [1137], 'Res.': [1138, 1310, 1340, 1356, 1381, 1398, 3771, 3787, 3812, 3829, 4511, 4527, 4552, 4569], '213:': [1140], '353-359Crossref': [1141], '(31)': [1144, 1424, 3855, 4595], 'regulation': [1148, 4837], 'CMP-NeuGc': [1152], 'expressions': [1153], 'rat': [1157], 'dependent': [1163, 4755], 'on': [1164, 1522, 1697, 1761, 2896, 3025, 3336, 3495, 4176], 'activity(14Kawano': [1170], 'barely': [1208], 'detectable': [1209], 'brain': [1212, 1222, 3614, 3661, 4292, 4348, 4477], 'mammals,': [1215], 'although': [1216, 4898], 'content': [1220], 'quite': [1224, 3745], 'high(21Iwamori': [1225], 'Nagai': [1227, 3901, 4318, 4392], '1978;': [1232, 4323], '528:': [1233, 4324], '257-267Crossref': [1234, 4325], '(168)': [1237, 4328], 'Polysialic': [1240], 'N-CAM': [1243, 4359], 'gangliosides,': [1245, 3615], 'regarded': [1248], 'functionally': [1251, 4353, 4449], 'important': [1252, 1557, 4450], 'neural': [1254], 'tissues,': [1255, 1604], 'contain': [1256, 1281, 1477, 3730, 4002, 4131], 'Thus,': [1261], 'brain-specific': [1262], 'suppression': [1263], 'evident': [1267], 'its': [1269, 1599, 3028, 3994], 'functional': [1270], 'role': [1271, 1491], 'unknown.': [1273], 'Normal': [1274], 'chicken': [1275], 'do': [1279, 4779], 'occurrence': [1284, 1434], 'T': [1289], 'activated': [1291], 'vitro': [1293], '(22Wakamiya': [1294], 'Osada': [1296], 'Wang': [1298], 'Nakajima': [1300, 1507], 'Kurimura': [1304], 'Commun.': [1311, 1357, 3788, 4528], '181:': [1313], '310-315Crossref': [1314], '(3)': [1317], 'types': [1322], 'cancer': [1325, 1573], '(23Hirabayashi': [1327, 3758], 'Higashi': [1329, 1391, 3760, 3822, 4500, 4562], 'Taniguchi': [1333, 3764, 4504], 'Jpn.': [1337, 3768, 4508], 'Cancer': [1339, 1380, 1397, 2266, 3770, 3811, 3828, 4510, 4551, 4568], '78:': [1342, 3773, 4513], '614-620PubMed': [1343, 3774, 4514], '24Higashi': [1346, 3777, 4517], 'Matuo': [1350, 3781, 4521], 'Okouchi': [1352, 3783, 4523], '79:': [1359, 3790, 4530], '388-395Crossref': [1360, 3791, 4531], '(163)': [1363, 3794, 4534], '25Higashi': [1366, 3797, 4537], 'Hirabayashi': [1368, 3799, 4539], 'Fukui': [1370, 3801, 4541], 'Ueda': [1376, 3807, 4547], '1985;': [1382, 3813, 4553, 4915], '45:': [1383, 3814, 4554], '3796-3802PubMed': [1384, 3815, 4555], '26Kawai': [1387, 3818, 4558], '51:': [1400, 3831, 4571], '1242-1246PubMed': [1401, 3832, 4572], '27Miyake': [1404, 3835, 4575], 'Ito': [1408, 3839, 4579], 'Ogawa': [1410, 3841, 4581], 'O.': [1411, 3842, 4582], 'Arai': [1412, 3843, 4583], 'Hitomi': [1414, 3845, 4585], 'Kannagi': [1416, 3847, 4587], '65:': [1420, 3851, 4591], '499-505Crossref': [1421, 3852, 4592], 'immunological': [1430], 'methods.': [1431], 'Although': [1432, 3279, 4487], 'remains': [1437], 'confirmed': [1440, 2178, 3529, 4284], 'chemical': [1442], 'methods,': [1443], 'it': [1444, 2858, 4103], 'possible': [1446, 4967], 'would': [1449, 3703], 'excellent': [1452], 'cancer-associated': [1453], 'antigen': [1454], 'tumor': [1456], 'marker.': [1457], 'specialized': [1459], 'physiological': [1460], 'functions': [1461, 4986], 'clear': [1468], 'at': [1469, 1686, 1802, 2146, 2391, 2416, 2578, 2628, 2644, 2747, 2773, 3292, 3306, 3328, 3596, 4458, 4907], 'present.': [1470], 'Influenza': [1471], 'virus': [1472], 'hemeagglutinins': [1473], 'bind': [1474], 'different': [1482], 'affinities,': [1483], 'neuraminic': [1486], 'play': [1489], 'determining': [1493], 'susceptibility': [1494], 'viral': [1496], 'infection(28Nobusawa': [1497], 'Aoyama': [1499], 'Tateno': [1505], 'Virology.': [1509], '182:': [1511], '475-485Crossref': [1512], '(431)': [1515], 'cell': [1519, 4408], 'adhesion': [1520], 'molecule': [1521], 'marginal': [1523], 'zone': [1524], 'macrophages,': [1525], 'sialoadhesin,': [1526], 'preferentially': [1527], 'recognizes': [1528, 4018], 'NeuAc-Gal-GalNAc': [1529], 'structure': [1530, 1582, 3979, 4802], 'NeuGc-Gal-GalNAc(29Kelm': [1533], 'Pelz': [1535], 'Crocker': [1539], 'P.R.': [1540], '330Crossref': [1545], 'These': [1548, 3742], 'examples': [1549], 'suggest': [1550, 4148], 'difference': [1554], 'biologically': [1556], 'recognition': [1559, 1800, 2144], 'events': [1560], 'mediated': [1561], 'carbohydrates.': [1563], 'To': [1564, 2119, 3567, 4243], 'elucidate': [1565], 'mechanisms': [1567, 4992], 'species-': [1569, 4722], 'tissue-specific': [1571, 3579, 4724], 'associated': [1574], 'regulations': [1575], 'expression,': [1578], 'information': [1579], 'about': [1580, 3249], 'required.': [1587, 4265], 'Here': [1588], 'we': [1589, 3701], 'report': [1590], 'cloning': [1593], 'mRNA': [1600, 3584, 3653, 3672, 4208, 4272, 4340, 4639], 'detection': [1606, 4610], 'several': [1612, 2037, 3754, 3862, 4286], 'species.': [1614, 2696], 'Purification': [1615], 'previously(15Kawano': [1621], 'An': [1641, 2171], 'aliquot': [1642, 2948], '(37': [1643], 'μg)': [1644], 'finally': [1647, 2752], 'lyophilized': [1651], 'then': [1653, 1948, 2526, 2634, 2893, 3081], 'dissolved': [1654], '40': [1656], 'μl': [1657, 1666], '8': [1659], 'M': [1660, 1669, 2413], 'urea.': [1661], 'After': [1662, 1920, 2733, 5000], 'dilution': [1663], '120': [1665], '0.1': [1668, 2638], 'Tris-HCl': [1670], 'buffer,': [1671, 2603], 'pH': [1672, 2597, 2604], '9.0,': [1673], '0.4': [1674], 'μg': [1675, 1753, 1852, 2509], '(Wako': [1678, 2494], 'Pure': [1679, 1707, 2495], 'Chemicals)': [1680, 2496], 'added,': [1682], 'followed': [1683], 'incubation': [1685], '37°C': [1687, 1888], 'overnight.': [1688], 'resulting': [1690], 'fragments': [1692, 1930, 2079, 2152, 3200, 3241], 'separated': [1694], 'HPLC': [1696, 2427], 'Wakosil': [1699], '5C18': [1700], 'column': [1701, 2432], '(4': [1702], '×': [1703, 2296, 2541, 2584, 2587, 2623, 2639, 2742], '150': [1704, 2589], 'mm;': [1705], 'Wako': [1706], 'Chemicals).': [1708], 'Elution': [1709], 'performed': [1711, 1750, 1827, 2018, 2050, 2576, 2703, 2716, 2922], 'linear': [1714], 'concentration': [1715, 2452], 'gradient': [1716, 2236], 'acetonitrile,': [1718], '0-60%,': [1719], '0.1%': [1721, 2626, 2642, 2745], 'trifluoroacetic': [1722], 'acid,': [1723], '60': [1725, 2325, 5001], 'min.': [1726, 2648], 'Twelve': [1727], 'peaks': [1729], 'recovered': [1731], 'subjected': [1733, 1932, 2344, 2515, 2956, 5012], 'sequencing': [1736, 1747, 2011, 3337], 'using': [1737, 1751, 1817, 1828, 1897, 2085, 2487, 2564, 3184, 4978], 'Applied': [1739, 1786], 'Biosystems': [1740, 1787], '470A': [1741], 'sequencer.': [1743], 'NH2-terminal': [1744, 2929], 'also': [1749, 3177, 4650, 4704, 4764, 4821, 5078], '6': [1752], 'Degenerate': [1758], 'oligonucleotides': [1759, 2087], 'designed': [1760, 2822, 2926], 'basis': [1763], 'lysylendopeptidase-digested': [1771, 3367], 'intact': [1775], 'enzyme,': [1776, 3375, 4836, 4977], 'shown': [1778, 2789, 3159, 3275, 3503, 3721], 'Fig.': [1780, 2791, 2835, 2909, 3097, 3161, 3277, 3398, 3723], '1A,': [1781, 2792], 'synthesized': [1783, 2914, 3091], '391': [1788], 'DNA': [1789, 1841, 2028, 2054, 2317, 2568, 2680], 'synthesizer.': [1790], 'Each': [1791], 'primer': [1793, 2035, 2567, 2985], 'either': [1795, 2134, 4894], 'EcoRI': [1797, 2671, 3062, 3074], 'HindIII': [1799], '5′-end.': [1804], 'pool': [1806], 'prepared': [1810, 2486, 2545, 2846, 3112, 3187], 'reverse': [1812, 3114, 3189], 'transcription': [1813, 3115, 3190], 'random': [1815, 2566], 'primers': [1816, 1832, 2040, 2802, 2821, 2902, 3084, 3958], 'total': [1820], 'RNA': [1821, 2481, 2512], 'template.': [1824], 'PCR': [1825, 1862, 1906, 1928, 2077, 2920, 2960, 2969, 3065, 3132, 3152], 'sense': [1829], 'antisense': [1831], '(125': [1833], 'pmol': [1834], 'each),': [1835], '1.3': [1837], 'units': [1838], 'Taq': [1840, 2027, 2067], '(Promega)': [1843], 'mixture': [1847], '(25': [1848], 'μl)': [1849], 'containing': [1850, 2523, 2550], '1': [1851, 1890, 2622, 2741], 'cDNA(30Innis': [1854], 'M.A.': [1855, 2962, 3125], 'Gelfand': [1856, 2963, 3126], 'D.H.': [1857, 2964, 3127], 'Sninsky': [1858, 2965, 3128], 'J.J.': [1859, 2966, 3129], 'White': [1860, 2967, 3130], 'T.J.': [1861, 2968, 3131], 'Protocols:': [1863, 2970, 3133], 'Guide': [1865, 2972, 3135], 'Methods': [1867, 2974, 3137], 'Applications.': [1869, 2976, 3139], 'Academic': [1870, 2977, 3140], 'Press,': [1871, 2004, 2254, 2320, 2978, 3141], 'Inc.,': [1872, 2979, 3142], 'York1990Google': [1874, 2981, 3144], 'Amplification': [1876], '30': [1881], 'cycles': [1882, 2233], '94°C': [1884], '0.5': [1886], 'min,': [1887, 1891, 1896, 2632], '72°C': [1893], '1.5': [1895, 3000], 'Zymoreactor': [1899], 'II': [1900, 1952, 2169, 2890, 3078], 'thermal': [1901, 2015], 'cycler': [1902, 2016], '(Atto': [1903], 'Corp.).': [1904, 2573], 'products': [1907], 'analyzed': [1909], '2%': [1911], 'agarose': [1912, 2521], 'gel': [1913, 1943, 2522, 3423], 'electrophoresis': [1914, 2044, 2517, 3424], 'stained': [1916], 'ethidium': [1918], 'bromide.': [1919], 'being': [1921], 'digested': [1922, 2158, 2669, 3068], 'appropriate': [1924], 'restriction': [1925], 'enzymes,': [1926], 'amplified': [1929, 2078, 2224, 2812, 2842, 2884, 3150, 3178], 'electrophoresis,': [1934], 'excised': [1935], 'gel,': [1938], 'QIAEX': [1942], 'extraction': [1944], 'kit': [1945, 2493, 2570], '(Qiagen),': [1946], 'subcloned': [1949, 2887], 'into': [1950, 1959, 2166, 2189, 2888, 3072, 4484], 'Bluescript': [1951, 2168, 2889, 3077], 'KS+': [1953, 2891], '(Stratagene).': [1954], 'constructs': [1956], 'transfected': [1958, 2302, 3510], 'XL1-Blue': [1960], 'strain.': [1961], 'Clones': [1962], 'inserts': [1965], 'identified': [1967], 'color': [1969], 'selection': [1970], 'isopropyl-1-thio-β-D-galactopyranoside': [1972], '5-bromo-4-chloro-3-indolyl-β-D-galactoside,': [1974], 'plasmid': [1976, 2306], 'DNAs': [1977, 3729, 3739], 'isolated': [1979, 3585], 'Escherichia': [1981], 'coli': [1982], 'grown': [1983, 2270], 'small': [1985], 'scale': [1986], 'cultures(31Sambrook': [1987], 'Fritsch': [1989, 2239], 'E.F.': [1990, 2240], 'Maniatis': [1991, 2241], 'Molecular': [1993, 2243], 'Cloning:': [1994, 2244], 'Laboratory': [1996, 2003, 2246, 2253], 'Manual.': [1997, 2247], '2nd': [1998, 2248], 'Ed.': [1999, 2249], 'Cold': [2000, 2005, 2250, 2255], 'Spring': [2001, 2006, 2251, 2256], 'Harbor': [2002, 2252], 'Harbor,': [2007, 2257], 'NY1989Google': [2008, 2258], 'Dye': [2020], 'Deoxy': [2021], 'terminators': [2022], '(Applied': [2023, 2030, 2056], 'Biosystems)': [2024], 'Ampli': [2026], 'Biosystems).': [2031, 2057], 'M13': [2033], 'universal': [2034], 'synthetic': [2038], 'used.': [2042], 'Gel': [2043], '373A': [2053], 'sequencer': [2055], 'Three': [2058], 'clones': [2059], 'sequenced': [2061], 'compensate': [2063], 'misreading': [2065], 'polymerase.': [2068], 'case': [2071], '5′-non-coding': [2074], 'sequence,': [2075, 2186, 2898, 2932], 'directly': [2081, 4808], 'sequenced,': [2082, 3214, 3287], 'without': [2083], 'subcloning,': [2084], 'Hydropathy': [2090], 'indexes': [2091], 'Kyte-Doolittle': [2093], '(32Kyte': [2094, 3464], 'Doolittle': [2096, 3466], 'R.F.': [2097, 3467], 'Mol.': [2099, 3469], '1982;': [2101, 3471], '157:': [2102, 3472], '105-132Crossref': [2103, 3473], '(16899)': [2106, 3476], 'estimated': [2110], 'sliding': [2113], 'window': [2114], 'nine': [2116], 'acids.': [2118], 'amplify': [2120], 'entire': [2122, 3966], 'region': [2124, 2141, 2554, 3013, 3282], 'primers,': [2129], 'each': [2130, 3298], 'contains': [2133, 4098], '5′-': [2136, 3020, 3251, 3974], '3′-end': [2138, 3172], 'BamHI': [2143, 2160, 2190], '5′-end,': [2148], 'synthesized.': [2150], 'Amplified': [2151], '(Takara': [2161, 2500, 2571], 'Shuzo': [2162, 2501, 2572], 'Corp.)': [2163], 'inserted': [2165], 'vector.': [2170], 'insert,': [2172], 'cloned': [2185, 3513, 3547, 3556], 'ligated': [2188, 3071], 'site': [2191, 3075, 3309, 4016], 'vector,': [2195], 'pdKCR(33Fukunaga': [2196], 'Sakawa': [2198], 'Nagata': [2200], 'Proc.': [2202], 'Natl.': [2203], 'Acad.': [2204], 'Sci.': [2205], '1984;': [2209], '81:': [2210], '5086-5090Crossref': [2211], '(28)': [2214], 'correct': [2221], 'direction': [2222], 'large': [2227], 'scale,': [2228], 'CsCl': [2235], 'centrifugation(31Sambrook': [2237], 'Japanese': [2265], 'Research': [2267], 'Bank': [2268], "Dulbecco's": [2272], "Eagle's": [2274], 'medium': [2275], '(Nissui': [2276], 'Pharmaceutical': [2277], 'Co.': [2278], 'Ltd.)': [2279], '10%': [2281], 'fetal': [2282], 'calf': [2283], 'serum': [2284], '(Life': [2285], 'Technologies,': [2286], 'Inc.)': [2287], 'kanamycin.': [2289], 'Six': [2290], 'dishes': [2291], '(5': [2295], '105': [2297], 'cells/10': [2298], 'cm': [2299], 'dish)': [2300], '(20': [2307], 'μg/dish)': [2308], 'calcium': [2310], 'phosphate': [2311, 2602], 'precipitation': [2312], 'methods(34Gorman': [2313], 'C.': [2314, 4117], 'Glover': [2315], 'D.M.': [2316], 'Cloning.': [2318], 'IRL': [2319], 'Oxford1985:': [2321], '143-190Google': [2322], 'At': [2324, 4200], 'h': [2326], 'incubation,': [2328], 'harvested,': [2332], 'washed,': [2333], 'collected': [2335], 'centrifugation.': [2337], 'One-fifth': [2338], 'pelleted': [2341, 2380, 2437], 'determination': [2347, 2449], 'according': [2352], 'Hara': [2354], 'et': [2355], 'al.(35Hara': [2356], 'Yamaguchi': [2358], 'Takemori': [2360], 'Furuhata': [2362], 'Ogura': [2364], 'Nakamura': [2366], 'Anal.': [2368], '179:': [2371], '162-166Crossref': [2372], '(246)': [2375], 'Briefly,': [2378], 'hydrolyzed': [2383], '0.9': [2385], 'ml': [2386, 2404], '0.05': [2388], 'N': [2389], 'H2SO4': [2390], '80°C': [2392], '3': [2394, 2508, 3086, 3118, 3181], 'h,': [2395, 5002], 'released': [2397], 'treated': [2401], '3.6': [2403, 3649, 3668], '3.': [2406], '5': [2407, 2583], 'mM': [2408, 2590, 2594, 2600], '1,2-diamino-4,5-methylenedioxybenzene': [2410], '1.7': [2412], 'acetic': [2414], '50°C': [2417], '2.5': [2419], 'h.': [2420], 'determined': [2425, 3420], 'TSK-gel': [2430], 'ODS-80TM': [2431], '(Tosoh).': [2433], 'Four-fifths': [2434], 'homogenized,': [2440], 'previously': [2458], '(14Kawano': [2460], 'Poly(A)+': [2480], 'ISOGEN': [2489], 'phenol': [2491], 'procedure': [2492], 'Oligotex': [2498], 'dT30': [2499], 'Corp.),': [2502], 'following': [2503, 3094, 4150], "manufacturers'": [2505], 'protocols.': [2506], 'Approximately': [2507, 3196], 'poly(A)+': [2511], 'samples': [2513], '1%': [2520], 'formaldehyde': [2524], 'transferred': [2527], 'MagnaGraph': [2530], 'nylon': [2531], 'filter': [2533], '(Micron': [2534], 'Separations': [2535], 'Inc.).': [2536], 'radiolabeled': [2538, 2700, 3601], '(3': [2540], '109': [2542], 'counts/min/μg)': [2543], 'gel-purified': [2548], 'whole': [2552, 3011], '2.0-kb': [2559], 'β-actin': [2561], '(Clontech)': [2563], 'labeling': [2569], 'Hybridization': [2574, 2714], 'overnight': [2577, 2734], '42°C': [2579], '50%': [2581], 'formamide,': [2582], 'SSC': [2585, 2624, 2640, 2743], '(1': [2586], 'SSC,': [2588], 'NaCl,': [2591], '15': [2593], 'sodium': [2595, 2601], 'citrate,': [2596], '7.0),': [2598], '50': [2599], '7.0,': [2605], '0.5%': [2606], 'skim': [2607], 'milk,': [2608], '1.5%': [2609], 'SDS,': [2610], '100': [2612, 3970], 'μg/ml': [2613], 'yeast': [2614], 'RNA.': [2615], 'washed': [2619, 2739], 'twice': [2620], 'SDS': [2627, 2643, 2746], '65°C': [2629, 2645], '10': [2631, 3651, 3670], 'three': [2635, 3300], 'times': [2636], '20': [2647], 'visualized': [2652], 'quantified': [2654], 'Fujix': [2657, 2760], 'BAS': [2658, 2761], '2000': [2659, 2762], 'Bio-imaging': [2660, 2763], 'Analyzer': [2661], '(Fuji': [2662], 'Photo': [2663], 'Film).': [2664], 'BIOS': [2666, 2675], 'EVO': [2667], 'Blot': [2668], 'purchased': [2673], 'Laboratories.': [2676], 'blotted': [2681], 'adjusted': [2683], 'assure': [2685], 'copy': [2688], 'number': [2689], 'genome': [2692, 4618], 'available': [2693], 'seven': [2695], 'Preparation': [2697], 'way': [2707], 'analysis.': [2713], 'essentially': [2717], 'except': [2723, 3017, 3660], 'proportion': [2726], 'formamide': [2728], 'decreased': [2730], '37.5%.': [2732], 'hybridization,': [2735], 'room': [2748], 'temperature,': [2749], '50°C,': [2750], '65°C.': [2753, 2774], 'radioactivity': [2755], 'monitored': [2757], 'Analyzer.': [2764], 'Discrete': [2765], 'visible': [2768], 'only': [2769, 4210, 4758], 'after': [2770], 'washing': [2772], 'NH2': [2786, 3330, 3370], 'terminus': [2787, 3331, 3371], 'these': [2794, 3344, 4448], 'designing': [2799], 'mixed': [2800], 'PCR.': [2804], 'First,': [2805], 'fragment': [2807, 2838, 2885, 2998, 3006, 3058, 3148, 3168, 3205, 3212, 3222, 3226, 4648], '158': [2809], 'bp': [2810, 3971], 'sets': [2818], 'degenerated': [2820], 'b': [2827], '(pr.': [2828], '2': [2829, 2904], 'a-pr.': [2831, 2934, 3088, 3117, 3156], '1,': [2832, 2925, 2996], 'respectively,': [2833], '1A).': [2836], 'This': [2837, 3005, 3526], 'could': [2839, 3332, 4481], 'brain,': [2849, 3588], 'negative': [2855], 'control': [2856], 'because': [2857, 3747, 4207, 4968], 'express': [2861], 'hydroxylase(14Kawano': [2863], 'sequenced.': [2894, 3082, 3170], 'Based': [2895], 'completely': [2899, 3286], 'matched': [2900], '(a-pr.': [2903, 2992, 3085], 'pr.': [2906, 2924, 2995, 3180], '3,': [2907], 'see': [2908], '1B': [2910, 3098], '2)': [2912, 2993, 4185], 'experiments.': [2917], 'next': [2919], 'between': [2923, 4254, 4268], '1.': [2935], 'this': [2937, 3647, 3981, 4041, 4127, 4245, 4485], 'PCR,': [2938], 'band': [2941], 'visibly': [2943], 'amplified,': [2944], 'when': [2946, 4178], 'product': [2952, 3066, 3706], 'diluted': [2954], 'second': [2959], '(30Innis': [2961, 3124], 'corresponding': [2986], 'exactly': [2987], 'inner': [2990, 3061], 'kb': [3001, 3652, 3671], 'pairs': [3002], 'amplified.': [3004, 3202], 'seemed': [3007], 'cover': [3009], '3′-ends,': [3022], 'judged': [3024], 'comparison': [3026], 'size': [3029], 'hydroxylase(15Kawano': [3036], 'Since': [3056, 4748], 'had': [3059], 'site,': [3063], 'EcoRI,': [3070], 'KS+,': [3079], 'Antisense': [3083], '4)': [3089], 'experiments': [3095, 4248], '(see': [3096], '2).': [3100, 3399], 'For': [3101, 4355], '5′-end': [3105, 3123], 'cDNA,': [3108, 4981], 'template': [3110], 'polyadenylation': [3120, 3714], '650-bp': [3147], 'oligo(dT)': [3154, 3192], '4,': [3157, 3724], '1B,': [3162], 'part': [3165, 3208], 'oligo(dT),': [3183], 'primer.': [3195], '250-': [3197], '700-bp': [3199, 3211, 3221], '250-bp': [3204, 3225], '89-bp': [3231], 'sequence.': [3233], 'merged': [3235], 'overlapping': [3239], 'frame,': [3247], '100-bp': [3250], '3′-untranslated': [3253, 3718, 3976], 'regions.': [3254, 3977], 'nucleotide': [3256, 4120, 4773], '(1731': [3258], 'bp)': [3259], '(577': [3266], 'acids)': [3268], '2.': [3278], '5′-untranslated': [3281], 'stop': [3288], 'codons': [3289], 'emerged': [3291], '−93,': [3293], '−59,': [3294], '−46': [3296], 'frames.': [3301], 'There': [3302], 'adjoining': [3304], 'methionines': [3305], 'potential': [3308, 3486], 'translation': [3311], 'initiation.': [3312], 'upper': [3314], 'methionine': [3315], 'tentatively': [3317], 'taken': [3318], 'initiation': [3321], 'site.': [3322], 'first': [3324], 'four': [3325], 'thus': [3343], 'cleaved,': [3348], 'possibly': [3349], 'post-translational': [3351, 4832], 'processing': [3352], 'artificial': [3354, 4955], 'cleavage': [3355], 'during': [3356, 4107], 'purification.': [3358], 'All': [3359], '12': [3366], 'residues': [3379], 'long': [3380], 'total,': [3382], 'found': [3384], '(underlined': [3396], 'calculated': [3403], 'kDa,': [3412], 'good': [3416], 'accordance': [3417], 'SDS-polyacrylamide': [3422], 'under': [3425], 'reducing': [3426], 'conditions': [3427], '(64': [3428], 'kDa)(15Kawano': [3429], 'search': [3450], 'revealed': [3454], 'hydropathy': [3462], 'plot': [3463], 'did': [3482, 4129], 'identify': [3484], 'transmembrane': [3487, 4095, 4132], 'region,': [3488], 'suggesting': [3489], 'translated': [3494], 'ribosomes': [3497], 'protein.': [3501], 'As': [3502, 3720], 'Table': [3505], 'I,': [3506], 'NeuGc,': [3520, 3681], 'compared': [3521], 'mock': [3524, 5007], 'transfectant.': [3525], 'change': [3527], 'due': [3532], 'increase': [3535], 'activity.': [3539], 'all': [3542, 3658, 4453], 'togehter': [3543], 'indicate': [3544], 'encodes': [3549], 'hydroxylase.Table': [3553], 'IExpression': [3554], 'Open': [3561], 'table': [3562], 'new': [3565], 'tab': [3566], 'test': [3568], 'possibility': [3570], 'regulates': [3577, 4710], 'NeuGc-containing': [3582, 4295, 4491, 4727, 4988], 'glycoconjugates,': [3583], 'thymus,': [3589], 'kidney,': [3591], 'spleen': [3593], 'probed': [3595], 'stringency': [3598], 'fragment.': [3603], 'It': [3604, 4455, 4631], 'hardly': [3610], 'whereas': [3616, 3733], 'expressed': [3622, 4790], 'other': [3625, 3987, 4796, 4826], 'tissues(18Nakamura': [3626], 'Consistent': [3645], 'observation,': [3648], '(Fig.': [3662, 4341], '3).': [3663, 4342], 'intensity': [3665], 'almost': [3675], 'expressing': [3680], 'brain.': [3690, 4470], '10-kb': [3692], 'transcript,': [3693], 'much': [3696], 'longer': [3697], 'than': [3698, 4827], 'obtained,': [3702], 'alternative': [3708, 3713], 'splicing': [3709], 'signals': [3715], 'region.': [3719], 'genomic': [3728], 'sequences,': [3732], 'chicken,': [3734], 'frog,': [3735], 'lobster,': [3736], 'mussel': [3738], 'negative.': [3741], 'interesting': [3746, 4634], 'cancerous': [3756, 4497, 4644], 'polysialoglycoproteins': [3860], 'kinds': [3863], '(36Kitajima': [3866], 'Inoue': [3868, 3870, 4671, 4673], 'Troy': [3872, 4675], 'F.A.': [3873, 4676], '18269-18276Abstract': [3879, 4682], 'chickens': [3889], 'frogs(37Hidari': [3891], 'Itonori': [3893], 'Sanai': [3895], 'Ohashi': [3897], 'Kasama': [3899], '412-416Crossref': [3908], '(19)': [3911], 'bivalves': [3921], 'date(38Vaskovsky': [3923], 'V.E.': [3924], 'Kostetsky': [3925], 'E.Y.': [3926], 'Svetashev': [3927], 'V.I.': [3928], 'Zhukova': [3929], 'I.G.': [3930], 'Smirnova': [3931], 'G.P.': [3932], 'Comp.': [3933], '1970;': [3936], '34:': [3937], '163-177Crossref': [3938], '(59)': [3941], 'Knowledge': [3944], 'enabled': [3953], 'us': [3954], 'design': [3956], 'generate': [3961], 'encoded': [3964], 'approximately': [3969], 'known': [3988, 4008], 'hydroxylases,': [3989], 'unique': [3995], 'substrate': [3996], 'specificity': [3997, 4906], 'CMP-NeuAc.': [3999], 'Several': [4000], 'sialyltransferases': [4001, 4887], 'conserved': [4004], '"sialylmotif,"': [4011], 'acid(39Datta': [4020], 'A.K.': [4021], 'Paulson': [4022, 4910], '1995;': [4027], '270:': [4028], '1497-11500Abstract': [4029], '(180)': [4037], 'motif': [4042], 'present': [4045, 4459], 'cytoplasm(15Kawano': [4056], 'systems': [4079], 'usually': [4081], 'located': [4082], 'microsomal': [4084, 4212], 'membranes.': [4085], 'Alternatively,': [4086], 'one': [4087], 'might': [4088], 'suppose': [4089], 'phosphatidylinositol-anchor': [4100], 'artificially': [4105], 'solubilized': [4106], 'purification': [4109], 'action': [4112], 'proteinases': [4114], 'phospholipase': [4116], 'However,': [4118], 'study': [4128], 'domain': [4133], 'insertion,': [4139], 'confirming': [4140], 'origin': [4143], 'enzyme.': [4146], 'models': [4151], 'explain': [4153], 'how': [4154], 'soluble': [4156, 4165, 4186, 4255], 'interacts': [4158, 4168], 'system.': [4163], '1)': [4164], 'short': [4171], 'time': [4172], 'microsomes,': [4177], 'transfer': [4180], 'occurs;': [4183], 'forms': [4187], 'NADH-cytochrome': [4192], 'reductase': [4194], 'reaction.': [4199], 'present,': [4201], 'latter': [4203], 'idea': [4204], 'unlikely': [4206], 'encoding': [4209], 'form': [4213], 'observed': [4218], 'liver(40Takematsu': [4221], 'Koyama': [4225], '115:': [4237], '381-386Crossref': [4238], 'clarify': [4244], 'point,': [4246], 'reconstitution': [4247], 'mimicking': [4249], 'vivo': [4252], 'interaction': [4253, 4362], 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'Montreuil': [4932], 'FEBS': [4936], 'Lett.': [4937], '250:': [4939], '245-250Crossref': [4940], '(25)': [4943], 'Modification': [4946], 'cultured': [4961], 'now': [4966], 'availability': [4970], 'cDNA.': [4972], 'Further': [4973], 'studies': [4974], 'facilitate': [4983], 'elucidation': [4984], 'ontogeny': [4997], 'phylogeny.': [4999], 'transfectant': [5004, 5008], 'harvested': [5010], 'assay.': [5022], 'fractions': [5029], 'measured.': [5031], 'grateful': [5034], 'Drs.': [5036], 'Kiyomitsu': [5037], 'Nara,': [5038], 'Hideo': [5039], 'Kubo,': [5040], 'Yoko': [5042], 'Nadaoka': [5043], 'Tokyo': [5046], 'Metropolitan': [5047], 'Institute': [5048], 'Medical': [5050], 'Science': [5051], 'suggestions': [5054], 'technical': [5056, 5072], 'support,': [5057], 'Dr.': [5059], 'Hiroshi': [5060], 'Kurata,': [5061], 'Yukiko': [5062], 'Tamagawa,': [5063], 'Mitsuyo': [5065], 'Ohkawa': [5066], 'Kyoto': [5068], 'University': [5069], 'advice': [5073], 'support.': [5075], 'authors': [5077], 'wish': [5079], 'thank': [5081], 'Prof.': [5082, 5091], 'Donald': [5083], 'Marcus': [5085], 'revising': [5087], 'manuscript': [5089], 'Tamio': [5092], 'his': [5095], 'encouragement.': [5096]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2046065497', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 9}, {'year': 2019, 'cited_by_count': 6}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 6}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 7}, {'year': 2012, 'cited_by_count': 8}], 'updated_date': '2025-01-11T12:55:26.225112', 'created_date': '2016-06-24'}