Publication Information

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3070, 3073, 3084, 3095, 3117, 3240, 3274, 3296, 3363, 3383, 3390, 3393, 3399, 3406, 3417, 3428, 3431, 3436, 3456, 3459, 3477, 3482, 3485, 3511, 3520, 3528, 3536, 3563, 3578, 3584, 3639, 3645, 3649, 3655, 3697, 3713, 3727, 3745, 3774, 3793, 3797, 3812, 3828, 3832, 3843, 3850, 3867, 3886, 3894, 3926, 3931, 3935, 3956, 3959, 3968, 4029, 4037, 4060, 4068, 4073, 4077, 4098, 4101, 4151, 4163, 4171, 4192, 4242, 4247, 4258, 4266, 4271, 4275, 4291, 4304, 4328, 4338, 4361, 4370, 4376, 4379, 4383, 4389, 4400, 4405, 4411, 4432, 4446, 4466, 4506, 4542, 4558, 4563, 4567, 4580, 4595, 4600, 4614, 4625], 'active': [15, 25, 244, 254, 657, 668, 684, 980, 1005, 1444, 1644, 3314, 3407, 4437, 4559, 4604], 'site': [16, 26, 245, 255, 685, 981, 1006, 1445, 1645, 1820, 1832, 1849, 1855, 1859, 1888, 2015, 2871, 3408, 4605], 'of': [17, 37, 100, 118, 156, 208, 246, 266, 329, 347, 385, 437, 475, 498, 509, 556, 602, 610, 661, 672, 682, 691, 707, 722, 777, 782, 814, 825, 849, 854, 998, 1036, 1144, 1276, 1518, 1530, 1613, 1652, 1669, 1693, 1706, 1724, 1774, 1850, 1875, 1893, 1899, 1920, 1934, 1984, 1995, 2019, 2028, 2034, 2058, 2070, 2130, 2138, 2214, 2216, 2241, 2248, 2297, 2310, 2382, 2572, 2587, 2594, 2600, 2612, 2644, 2650, 2661, 2678, 2722, 2746, 2781, 2863, 2872, 2909, 2920, 2950, 2957, 3045, 3052, 3083, 3165, 3214, 3263, 3271, 3284, 3316, 3392, 3430, 3458, 3476, 3484, 3625, 3641, 3657, 3692, 3715, 3729, 3770, 3782, 3830, 3866, 3941, 3958, 4013, 4020, 4084, 4114, 4118, 4166, 4246, 4261, 4315, 4346, 4360, 4378, 4455, 4465, 4491, 4513, 4523, 4535, 4550, 4572, 4583, 4594, 4597, 4602, 4621, 4631, 4636], 'type': [18, 125, 148, 162, 180, 247, 354, 377, 391, 409, 539, 542, 561, 613, 779, 3111, 3133, 3242, 3487, 3699, 3705, 3756, 3784, 3799, 3834, 3852, 4153, 4273, 4280, 4306, 4340, 4434, 4477, 4508, 4585], 'IA': [19, 248, 540, 545, 614, 780], 'topoisomerases': [21, 250, 469, 547, 564, 574, 615, 783], 'and': [22, 51, 63, 74, 79, 92, 135, 220, 251, 280, 292, 303, 308, 321, 364, 449, 515, 541, 608, 646, 679, 760, 818, 912, 953, 990, 992, 1021, 1110, 1170, 1294, 1689, 1695, 1769, 1808, 1827, 1856, 1880, 1969, 1990, 2120, 2132, 2141, 2150, 2193, 2199, 2261, 2274, 2342, 2377, 2385, 2429, 2443, 2541, 2590, 2622, 2628, 2630, 2635, 2682, 2708, 2743, 2796, 2799, 2801, 2953, 3019, 3042, 3109, 3141, 3157, 3247, 3302, 3321, 3445, 3449, 3570, 3586, 3700, 3739, 3743, 3785, 3869, 4009, 4123, 4147, 4177, 4244, 4281, 4307, 4320, 4387, 4414, 4441, 4443, 4457, 4481, 4496, 4586, 4599, 4609, 4619], 'near': [23, 252, 1442], 'tyrosine.': [27, 256], 'Its': [28, 257], 'ability': [29, 56, 258, 285, 1223, 3226, 3250, 3429, 3457, 3746, 3865, 4623], 'to': [30, 45, 53, 57, 145, 259, 274, 282, 286, 374, 479, 485, 552, 569, 697, 704, 754, 862, 1172, 1270, 1299, 1356, 1409, 1411, 1495, 1538, 1542, 1550, 1577, 1637, 1665, 1683, 1719, 1760, 1770, 1860, 1941, 2066, 2134, 2206, 2244, 2284, 2371, 2914, 2992, 3027, 3033, 3036, 3102, 3116, 3149, 3211, 3222, 3269, 3279, 3389, 3416, 3433, 3442, 3454, 3461, 3522, 3531, 3544, 3577, 3607, 3627, 3747, 3791, 3795, 3818, 3853, 3872, 3918, 3954, 3965, 3976, 4036, 4142, 4158, 4186, 4216, 4230, 4368, 4374, 4391, 4505, 4547, 4553, 4557, 4617, 4645], 'participate': [31, 58, 260, 287, 1353, 3374], 'multistep': [34, 263, 618], 'catalytic': [35, 264, 1000, 1044, 1260, 4095], 'process': [36, 265, 1261], 'relaxation': [39, 73, 268, 302, 1203, 1222, 1290, 1614, 1701, 1989, 2433, 3137, 3225, 3237, 3249, 3278, 3543, 3558, 3576, 3591, 3663, 3695, 3733, 4622], 'was': [40, 43, 185, 269, 272, 414, 764, 1681, 1702, 1711, 1750, 1757, 1786, 1821, 1833, 1842, 1902, 1915, 1976, 2023, 2061, 2146, 2171, 2184, 2204, 2237, 2254, 2306, 2369, 2393, 2503, 2549, 2750, 2977, 3025, 3031, 3058, 3440, 3601, 3877, 3963, 4132, 4140, 4628], 'investigated.': [41, 270], 'mutated': [44, 273, 1682, 3441], 'alanine,': [46, 275, 1213, 1684, 3037], 'arginine,': [47, 276, 1685, 3038], 'asparagine,': [48, 277, 1687, 3040], 'aspartate,': [49, 278], 'glutamate,': [50, 279, 1688, 3041], 'glutamine': [52, 281, 1228, 4276, 4282, 4308], 'study': [54, 283], 'its': [55, 284, 4237, 4353], 'general': [60, 289, 1329, 1635, 1659, 3299, 3376, 3419, 3437, 3465, 3472, 3516], 'acid/base': [61, 290, 1332, 3300, 3377], 'catalysis': [62, 225, 291, 454, 1333, 3301, 3378], 'bind': [64, 293], 'DNA.': [65, 294, 725, 4017, 4569], 'The': [66, 84, 97, 182, 295, 313, 326, 411, 643, 701, 751, 808, 844, 889, 979, 1025, 1205, 1403, 1691, 1704, 1743, 1783, 1839, 1897, 1907, 2144, 2181, 2234, 2251, 2532, 2546, 2690, 2748, 2804, 3107, 3131, 3281, 3313, 3568, 3686, 3703, 3719, 3864, 4018, 4278, 4452, 4489, 4510], 'mutants': [67, 85, 102, 296, 314, 331, 1924, 1963, 1985, 1994, 3047, 3091, 3537, 3564, 3673, 3721, 3776, 3813, 3845, 3871, 4362, 4627], 'were': [68, 297, 1964, 1999, 2043, 2074, 2299, 2336, 2348, 2451, 2456, 2534, 2665, 2693, 2785, 2807, 2826, 2835, 2912, 2932, 2944, 2968, 2990, 3092, 3100, 3452, 3619, 3665, 3789, 4125, 4643], 'examined': [69, 298, 3878, 4126], 'for': [70, 114, 223, 299, 343, 452, 495, 504, 1289, 1314, 1623, 1917, 1966, 1970, 1981, 2124, 2228, 2431, 2438, 2447, 2565, 2605, 2670, 2687, 2922, 2974, 3277, 3603, 3999, 4214, 4450, 4590], 'pH-dependent': [71, 90, 128, 300, 319, 357, 3145, 3266, 3611], 'cleavage,': [75, 130, 304, 359, 1293, 2782, 4168, 4578], 'salt-dependent': [76, 80, 305, 309], 'relaxation,': [78, 307], 'binding': [82, 175, 183, 202, 219, 311, 404, 412, 431, 448, 897, 961, 1117, 1355, 1488, 1745, 1763, 1972, 2440, 2988, 3017, 4070, 4102, 4176, 4549, 4571, 4581, 4608, 4638, 4649], 'affinity.': [83, 312, 4650], 'relax': [86, 315, 1216, 3113, 3677, 3708, 3748, 3819, 3854], 'a': [89, 153, 172, 198, 318, 382, 401, 427, 549, 566, 580, 584, 617, 648, 673, 775, 822, 886, 914, 919, 954, 1227, 1328, 1381, 1385, 1482, 1531, 1540, 1610, 1634, 1658, 1663, 1670, 1828, 1876, 1881, 1885, 1979, 2012, 2049, 2067, 2077, 2135, 2162, 2187, 2245, 2269, 2280, 2372, 2401, 2597, 2658, 2696, 2812, 2831, 2870, 2906, 2961, 2984, 3062, 3080, 3142, 3305, 3308, 3327, 3387, 3403, 3414, 3423, 3464, 3471, 3490, 3498, 3502, 3515, 3532, 3539, 3632, 3767, 3920, 3977, 4089, 4207, 4343, 4392, 4521, 4554, 4591, 4629, 4632], 'manner': [91, 320, 2353], 'low': [94, 323, 4344], 'salt': [95, 189, 324, 418, 1696, 1767, 2566, 3682, 3693, 3771, 3805, 3859, 4618], 'concentrations.': [96, 325], 'pH': [98, 116, 327, 345, 1694, 1715, 2607, 2620, 2626, 2633, 3139, 3153, 3159, 3217, 3230, 3245, 3252, 3282, 3494, 3546, 3566, 3593, 3600, 3651, 4128, 4232, 4256, 4288, 4318, 4349, 4425, 4439, 4460, 4473, 4502], 'dependence': [99, 117, 328, 346, 2567, 3283, 4161, 4448, 4461], 'all': [101, 330, 506, 3841], 'different': [104, 188, 333, 417, 1714, 1766, 4424], 'from': [105, 334, 1642, 1788, 1814, 1912, 1925, 2007, 2148, 2257, 2395, 2453, 2490, 2527, 3405, 4026], 'wild': [107, 124, 147, 161, 179, 336, 353, 376, 390, 408, 2645, 3110, 3132, 3241, 3486, 3609, 3698, 3704, 3783, 3798, 3833, 3851, 4152, 4272, 4279, 4305, 4339, 4433, 4476, 4507, 4584], 'type,': [108, 337, 2646], 'suggesting': [109, 338, 4169], 'that': [110, 192, 214, 339, 421, 443, 763, 884, 917, 958, 1034, 1252, 1273, 1484, 1534, 1544, 1573, 2495, 3069, 3121, 3295, 3303, 3385, 3401, 3506, 3689, 3811, 3938, 4032, 4081, 4170, 4203, 4210, 4302, 4337, 4382, 4431], 'responsible': [113, 222, 342, 451], 'enzyme.': [120, 181, 349, 410, 699, 4509], 'Additionally,': [121, 350], 'whereas': [122, 150, 351, 379, 560, 687, 3826, 3925, 4100, 4137, 4290], 'enzyme': [126, 355, 644, 702, 731, 745, 752, 3134, 3297, 3488, 3500, 3706, 3800, 4014, 4038, 4085, 4108, 4138, 4197, 4217, 4227, 4341, 4544, 4564], 'shows': [127, 356, 3135], 'oligonucleotide': [129, 358, 1709, 2446, 2721, 2862, 4469, 4492, 4514], 'cleavage': [131, 158, 360, 387, 1710, 1887, 1991, 2235, 2449, 2878, 3923, 3929, 3946, 4006, 4074, 4178, 4262, 4316, 4347, 4371, 4453, 4470, 4493], 'by': [132, 361, 612, 717, 995, 1778, 1904, 2063, 2076, 2096, 2128, 2153, 2173, 2239, 2264, 2308, 2379, 2536, 2675, 2710, 2809, 2828, 3239, 3412, 3562, 3615, 3696, 3980, 4265, 4270, 4475, 4494], 'both': [133, 362, 756, 4174, 4303], 'H365Q': [134, 139, 363, 368, 3870, 4155, 4458, 4482, 4497], 'H365A': [136, 151, 169, 365, 380, 398, 3614, 3635, 3646, 3868, 4181, 4204, 4234, 4324, 4456, 4479, 4495], 'pH-independent.': [138, 367], 'cleaves': [140, 369], 'with': [142, 371, 1211, 1226, 1414, 1437, 1523, 1823, 1835, 1872, 2313, 2487, 2505, 2544, 2705, 2726, 2916, 2946, 3094, 3501, 3548, 3589, 3613, 3667, 3879, 3890, 3951, 4191, 4195], 'rates': [143, 372, 4080, 4419], 'similar': [144, 373, 3491, 3975], 'enzyme,': [149, 378, 856], 'has': [152, 171, 381, 400, 638, 859, 881, 1147, 1155, 1262, 3648, 3762, 3898, 4235, 4294, 4342, 4537], 'slower': [154, 383], 'rate': [155, 384, 1705, 2780, 4062, 4091, 4193, 4243, 4355, 4377, 4471, 4490, 4511], 'than': [159, 177, 204, 388, 406, 433, 3583, 3773, 3849, 4269, 4358], 'but': [163, 392, 1219, 1605, 3489, 3671, 4351], 'can': [164, 393, 3707, 4249, 4420, 4428, 4565], 'cleave': [165, 394, 471, 575, 3873, 4286, 4566], 'more': [166, 395, 3550, 3582, 3838, 4356, 4436], 'substrate': [167, 396, 4022], 'overall.': [168, 397], 'also': [170, 399, 1758], 'lower': [173, 402, 3533, 3599], 'affinity': [176, 184, 405, 413, 898, 1746, 1764, 2191, 2272, 2441, 4582], 'determined': [186, 415], 'concentrations,': [190, 419], 'showing': [191, 420], 'alanine': [194, 423, 1578, 3444, 3585, 4267, 4292], 'mutant': [195, 424, 2010, 2041, 2295, 2651, 3499, 3647, 3701, 3787, 3802, 4268, 4283, 4293, 4309, 4484, 4587], 'displaces': [196, 425], 'half': [197, 426], 'charge': [199, 428, 1549], 'less': [200, 429, 3816, 3847], 'upon': [201, 430, 1781, 4501], 'an': [205, 434, 1212, 1620, 1624, 2727, 2935, 2947, 2954, 3443, 3446, 3523, 3880, 3891, 3961], 'inactive': [206, 435], 'form': [207, 436, 548, 885, 3082], 'I.': [210, 439, 4488], 'These': [211, 440, 1962], 'observations': [212, 441], 'indicate': [213, 442], 'participates': [216, 445, 1675], 'optimal': [224, 453, 3574], 'physiological': [227, 456], 'pH.': [228, 457, 3534], 'glutathione': [458, 1942], 'S-transferase': [459, 1943], 'fluorescence': [460, 1753, 4640], 'polarization': [461, 1756, 2929, 4641], '4-morpholineethanesulfonic': [462], 'acid': [463, 465, 3384], '2-(cyclohexylamino)ethanesulfonic': [464], 'Type': [466, 530, 544], 'I': [467, 531, 773, 989, 1780, 1870, 1939, 2656, 2877, 3057, 3288, 3320, 3761, 3944], 'transiently': [470], 'phosphodiester': [473, 577, 1359], 'backbone': [474, 1360], 'one': [476, 762], 'strand': [478, 606, 710, 728, 759, 1656, 4031], 'allow': [480, 705, 1536, 4548], 'single-': [481], 'or': [482, 714, 1292, 1378, 1439, 1490, 1673, 2362, 2647, 2855, 2981, 3228, 3267, 3307, 3410, 4072, 4087, 4274, 4634], 'double-stranded': [483], 'pass': [486], 'through': [487, 711, 766, 2268, 2279], 'break': [489], 'before': [490, 4577], 'resealing': [491], 'it.': [492], 'This': [493, 1266, 1616, 1864, 4201, 4651], 'allows': [494, 4589], 'interconversion': [497], 'topological': [499], 'isomers,': [500], 'which': [501, 1928, 3024, 3796, 4541], 'necessary': [503], 'nearly': [505], 'cellular': [507, 2151], 'transactions': [508], 'such': [511], 'as': [512, 821, 1012, 1028, 1030, 1327, 1380, 1633, 1657, 1667, 1978, 2354, 2458, 2752, 3061, 3379, 3382, 3398, 3435, 3463, 3470, 3514, 3653, 3804, 3858, 3893, 4028, 4449, 4520], 'replication,': [513], 'transcription,': [514], 'recombination': [516], '(1.Wang': [517, 784], 'J.C.': [518, 626, 785, 832, 868, 1049, 1064, 1180, 1232, 1419, 1588, 1794, 1948, 3332], 'Annu.': [519, 590, 786], 'Rev.': [520, 591, 787], 'Biochem.': [521, 592, 788, 941, 3199, 3990], '1996;': [522, 789, 2474, 2892], '65:': [523, 790], '635-692Crossref': [524, 791], 'PubMed': [525, 596, 633, 792, 839, 875, 907, 932, 948, 974, 1056, 1076, 1102, 1133, 1192, 1244, 1373, 1398, 1426, 1458, 1473, 1510, 1562, 1600, 1738, 1801, 1957, 2091, 2330, 2482, 2772, 2900, 3188, 3203, 3339, 3913, 3994, 4053], 'Scopus': [526, 597, 634, 793, 804, 840, 876, 908, 933, 949, 975, 1057, 1077, 1103, 1134, 1193, 1245, 1349, 1374, 1399, 1427, 1459, 1474, 1511, 1563, 1601, 1739, 1802, 1958, 2092, 2331, 2483, 2773, 2901, 3189, 3204, 3340, 3359, 3914, 3995], '(2088)': [527, 794], 'Google': [528, 599, 636, 795, 806, 842, 878, 910, 935, 951, 977, 1059, 1079, 1105, 1136, 1195, 1247, 1351, 1376, 1401, 1429, 1461, 1476, 1513, 1565, 1603, 1741, 1804, 1960, 2094, 2333, 2485, 2775, 2903, 3191, 3206, 3342, 3361, 3916, 3997, 4054], 'Scholar).': [529, 807, 843, 879, 978, 1106, 1196, 1248, 1402, 1477, 1514, 1742, 1961, 2334, 2776, 3207, 4055], 'enzymes': [532, 3099, 3788, 3803, 4156, 4588], 'are': [533, 1007, 1040, 1286, 3814, 3846, 4082], 'further': [534], 'divided': [535], 'into': [536, 1810, 1845], 'two': [537], 'subfamilies,': [538], 'IB.': [543], 'covalent': [550, 674], 'linkage': [551], '5′-phosphoryl': [554, 678], 'end': [555, 690], 'cleaved': [558, 724, 748, 2205, 2252, 4030, 4515], 'DNA,': [559, 1524], 'IB': [562], 'undergo': [565, 4206], 'transesterification': [567], 'reaction': [568, 2236, 2691, 2715, 2805, 3969, 4239, 4248, 4384], '3′-phosphoryl': [571], 'end.': [572, 3888], 'All': [573, 2942, 3098, 3535], 'bond': [578, 675], 'via': [579, 670, 1806, 2025, 3422], 'phosphotyrosine': [581], 'intermediate.': [582], 'For': [583, 1306], 'recent': [585], 'review': [586], 'see': [587], 'Ref.2.Champoux': [588], 'J.J.': [589, 2322], '2001;': [593, 1395, 1470, 1559, 1735, 3910], '70:': [594], '369-413Crossref': [595], '(2238)': [598], 'Scholar.': [600], 'Creation': [601], 'transient': [604], 'break,': [605], 'passage,': [607, 729], 're-ligation': [609, 4025], 'process.': [619], 'A': [620, 1816, 2413, 4365, 4533], 'proposed': [621, 4539], 'mechanism': [622, 1045, 4534, 4596], '(3.Lima': [623, 865, 1046, 1416, 3329], 'C.D.': [624, 866, 1047, 1417, 1946, 3330], 'Wang': [625, 831, 867, 1048, 1063, 1179, 1231, 1418, 1587, 1793, 1947, 3331], 'Mondragón': [627, 869, 1050, 1420, 1949, 3333], 'A.': [628, 870, 938, 1051, 1088, 1335, 1364, 1366, 1389, 1393, 1421, 1449, 1451, 1464, 1468, 1501, 1503, 1553, 1557, 1950, 3334, 3345], 'Nature.': [629, 871, 1052, 1394, 1422, 1469, 1558, 3335], '1994;': [630, 801, 872, 1053, 1423, 3336, 3991], '367:': [631, 873, 1054, 1424, 3337], '138-146Crossref': [632, 874, 1055, 1425, 3338], '(266)': [635, 877, 1058, 1428, 3341], 'Scholar)': [637, 911, 952, 1430, 1566, 1805, 2095, 2428, 2486, 3917, 3998], 'following': [640], 'steps.': [641], '1)': [642, 3381], 'recognizes': [645], 'binds': [647], 'single-stranded': [649, 663, 1707, 3874, 4467, 4551, 4568, 4573], 'region,': [651], 'positioning': [652], 'site.': [658, 2031, 4560], '2)': [659, 3397], 'Cleavage': [660], 'occurs': [665, 4010], 'site,': [669], 'formation': [671], 'between': [676, 987, 1852, 2619, 2625, 2632, 3735, 4331], 'hydroxyl': [681, 1640], 'tyrosine,': [686, 1001, 1646], '3′': [689], 'remains': [694], 'non-covalently': [695, 4034], 'bound': [696, 1441, 2370, 4035], '3)': [700, 3156, 3411], 'opens': [703, 753], 'passage': [706], 'other': [709], 'gap': [713], 'gate': [715, 741], 'created': [716], 'separating': [718], 'broken': [720], 'ends': [721], '4)': [726], 'Following': [727], 'closes,': [732], 'trapping': [733], 'passing': [735, 2265], 'inside.': [737], '5)': [738], 'Once': [739], 'closed,': [743], 're-ligates': [746], 'strand.': [749], '6)': [750], 'release': [755], 're-ligated': [758], 'passed': [765], 'gap.': [768], 'member': [776], 'subfamily': [781], 'Scholar,': [796, 936, 1060, 1080, 1462, 3192, 3343], '4.Tse-Dinh': [797], 'Y.C.': [798, 830, 902, 940, 967, 1090, 1121, 2760, 3176, 3196, 4043], 'Adv.': [799], 'Pharmacol.': [800], '29:': [802, 1736, 3911], '21-37Crossref': [803], '(16)': [805], '97-kDa': [809], 'protein': [810, 1871, 1998, 2060, 2145, 2183, 2203, 2217, 2253, 2260, 2267, 2595, 2921, 3085, 3243], 'product': [813, 1841, 2548], 'topA': [816, 1784], 'gene': [817, 1785], 'expressed': [820], 'single': [823, 3972, 4393], 'chain': [824], '865': [826], 'amino': [827], 'acids': [828], '(5.Tse-Dinh': [829], 'J.': [833, 926, 1065, 1091, 1122, 1181, 1233, 1589, 1732, 1795, 1951, 2406, 2471, 2761, 2889, 3177, 3197, 3907, 4044], 'Mol.': [834, 927, 1796, 1952], 'Biol.': [835, 928, 1066, 1092, 1123, 1182, 1234, 1369, 1454, 1506, 1590, 1733, 1797, 1953, 2472, 2762, 2890, 3178, 3908, 4045], '1986;': [836, 1798, 4047], '191:': [837, 1799], '321-331Crossref': [838], '(73)': [841], 'three-dimensional': [845, 1026], 'x-ray': [846], 'crystal': [847], 'structure': [848, 1027, 1517, 1967], '67-kDa': [851, 1922, 1931], 'N-terminal': [852, 1923, 1932, 3122], 'fragment': [853, 1933, 2502], 'residues': [857, 1011, 1039, 1146, 1254, 1278, 4606], '1–595,': [858], 'been': [860, 1113, 1149, 1156, 1264, 1312, 3899, 3949, 4538], 'solved': [861], '2.2-Å': [863], 'resolution': [864], 'It': [880, 1323, 4427], 'four': [882], 'domains': [883, 988], 'toroid-shaped': [887], 'molecule.': [888], 'remaining': [890], '30-kDa': [891], 'C': [892, 956], 'terminus': [893, 957, 1892], 'confers': [894], 'higher': [895], '(6.Beran-Steed': [899], 'R.K.': [900], 'Tse-Dinh': [901, 939, 966, 1089, 1120, 1729, 2759, 3175, 3195, 3904], 'Proteins.': [903], '1989;': [904], '6:': [905, 1371, 1456, 1508], '249-258Crossref': [906], '(46)': [909], 'contains': [913, 1929], 'tetracysteine': [915], 'motif': [916], 'forms': [918], 'putative': [920, 3418], 'zinc': [921], 'finger': [922], 'triplet': [923], '(7.Grishin': [924], 'N.V.': [925], '2000;': [929, 1125], '299:': [930], '1165-1177Crossref': [931], '(47)': [934, 976], '8.Ahumada': [937], 'Biophys.': [942, 2086], 'Res.': [943], 'Commun.': [944], '1998;': [945, 1068, 1094, 1184, 1236, 1592], '251:': [946], '509-514Crossref': [947], '(24)': [950], '14-kDa': [955], 'enhances': [959], '(9.Yu': [962], 'L.': [963, 1792], 'Zhu': [964], 'C.X.': [965, 1082, 1119, 2756, 3172], 'Fesik': [968], 'S.W.': [969], 'Biochemistry.': [970], '1995;': [971], '34:': [972], '7622-7628Crossref': [973], 'interface': [986], 'III': [991, 1521, 3323], 'identified': [994], 'presence': [997, 3640, 3714], 'Tyr-319.': [1002], 'Near': [1003], 'many': [1008, 1035, 1139], 'highly': [1009], 'conserved': [1010, 1038, 3365], 'follows:': [1013, 3380], 'Glu-9,': [1014, 1159, 3421], 'Asp-111,': [1015, 1108, 1161], 'Asp-113,': [1016, 1109], 'Glu-115,': [1017, 1162], 'Tyr-312,': [1018], 'Glu-313,': [1019, 1164], 'Arg-321,': [1020, 1166], '(Fig.': [1023, 1567, 3155, 3554, 3684, 3777, 3862, 4199, 4252, 4363, 4462], '1).': [1024], 'well': [1029], 'mutagenesis': [1031, 1154, 2005, 3030], 'studies': [1032, 1570, 2568, 3940], 'suggest': [1033], 'these': [1037, 1253, 3046, 3090], 'involved': [1041], '10.Chen': [1061], 'S.J.': [1062, 1178, 1230, 1586], 'Chem.': [1067, 1093, 1124, 1183, 1235, 1591, 2473, 2763, 2891, 3179, 4046], '273:': [1069, 1095, 1185, 1237, 1593], '6050-6056Abstract': [1070, 1186, 1238, 1594], 'Full': [1071, 1073, 1097, 1099, 1128, 1130, 1187, 1189, 1239, 1241, 1344, 1346, 1595, 1597, 2477, 2479, 2767, 2769, 2895, 2897, 3183, 3185, 3354, 3356, 4050], 'Text': [1072, 1074, 1098, 1100, 1129, 1131, 1188, 1190, 1240, 1242, 1345, 1347, 1596, 1598, 2478, 2480, 2768, 2770, 2896, 2898, 3184, 3186, 3355, 3357, 4051], 'PDF': [1075, 1101, 1132, 1191, 1243, 1348, 1599, 2481, 2771, 2899, 3187, 3358, 4052], '(57)': [1078, 1194, 1246, 1602], '11.Zhu': [1081], 'Roche': [1083, 2757, 3173], 'C.J.': [1084, 1728, 2758, 3174, 3903], 'Papanicolaou': [1085], 'N.': [1086], 'DiPietrantonio': [1087], '8783-8789Abstract': [1096], '(39)': [1104], 'Although': [1107, 3232], 'Glu-115': [1111], 'have': [1112, 1311, 1571, 3326, 3722, 3731, 3919, 3947, 4159, 4310], 'implicated': [1114], 'Mg(II)': [1116], '(12.Zhu': [1118], '275:': [1126], '5318-5322Abstract': [1127], '(58)': [1135], 'Scholar),': [1137, 1352, 1377, 1604], 'instances': [1140], 'specific': [1142], 'role': [1143, 1251, 1275, 4601], 'particular': [1145], 'not': [1148, 1200, 1263, 1280, 1580, 3059, 3087, 3105, 3125, 3129, 3162, 3255, 3325, 3496, 3622, 3660, 3675, 3948, 4285, 4499], 'definitively': [1150], 'determined.': [1151], 'Alanine': [1152], 'substitution': [1153], 'performed': [1157, 2751, 3666, 3790], 'on': [1158, 1698, 2695, 2811, 2830, 2934, 4107], 'His-33,': [1160], 'Gln-309,': [1163], 'Thr-318,': [1165], 'Thr-322,': [1167], 'Asp-323,': [1168], 'His-365,': [1169], 'Thr-496': [1171], 'identify': [1173], 'their': [1174, 1301, 4312], 'possible': [1175, 1309, 3060], 'roles': [1176, 1310], '(10.Chen': [1177, 1229, 1585], 'Most': [1197], 'mutations': [1198, 1726, 3588], 'do': [1199, 3124, 3324, 3674, 4284], 'completely': [1201], 'abolish': [1202, 1581], 'activity.': [1204, 1615, 3637, 4296], 'exception': [1206], 'Glu-9.': [1208], 'When': [1209], 'substituted': [1210], 'it': [1214, 1296, 1415, 1433, 1479, 1491, 1537, 3219, 3257, 3439, 4300, 4334, 4444], 'cannot': [1215], 'supercoiled': [1217, 1699, 2573, 2613, 3114, 3167, 3292, 3678, 3710, 3749, 3820, 3856], 'retains': [1220], 'reduced': [1221, 1611], 'when': [1224, 1284], 'replaced': [1225], 'Nevertheless,': [1249, 1478, 4241], 'may': [1255, 1279, 1535, 4205], 'be': [1256, 1281, 3150, 3311, 4105, 4212, 4250, 4421, 4429], 'playing': [1257], 'resolved.': [1265], 'partially': [1268], 'due': [1269, 3268], 'fact': [1272], 'some': [1277], 'immediately': [1282], 'apparent': [1283], 'they': [1285], 'only': [1287, 3364, 3836], 'tested': [1288], 'activity': [1291, 1584, 2434, 3127, 3238, 3264, 3483, 3530, 3559, 3597, 3612, 3725, 3734, 3765, 4454], 'hence': [1295], 'important': [1298], 'elucidate': [1300], 'involvement': [1302], 'greater': [1304, 3768, 4264, 4615], 'detail.': [1305], 'example,': [1307, 4215], 'several': [1308], 'suggested': [1313], 'residue': [1318, 1618, 1680, 3432, 3460, 3504, 3525], 'position': [1320, 1483, 3370, 3933], '365': [1321], '(His-365).': [1322], 'could': [1324, 1485, 1492, 1545, 3310, 3373, 4063, 4211], 'either': [1325, 3304, 4066, 4359], 'act': [1326, 3434, 3462, 3513], 'base': [1330, 1636, 3400, 3896], '(13.Mondragón': [1334], 'DiGate': [1336, 1390, 1465, 1554, 2469, 2887, 3346], 'R.': [1337, 3347], 'Struct.': [1338, 1368, 1453, 1505, 3348], 'Fold.': [1339, 3349], 'Des.': [1340, 3350], '1999;': [1341, 1370, 1455, 1507, 3351], '7:': [1342, 3352], '1373-1383Abstract': [1343, 3353], '(67)': [1350, 3360], '(14.Feinberg': [1361, 1446, 1498], 'H.': [1362, 1447, 1499, 2468, 2886], 'Changela': [1363, 1448, 1500], 'Mondragon': [1365, 1392, 1450, 1467, 1502, 1556], 'Nat.': [1367, 1452, 1504], '961-968Crossref': [1372, 1457, 1509], '(23)': [1375, 1460, 1512], 'serve': [1379], 'proton': [1382, 1541, 1641, 1664, 3388, 3404, 3415], 'donor': [1383], 'charge-relay': [1386, 1671, 3424], 'network': [1387, 1533], '(15.Changela': [1388, 1552], 'R.J.': [1391, 1466, 1555, 2470, 2888], '411:': [1396, 1471, 1560], '1077-1081Crossref': [1397, 1472, 1561], '(90)': [1400, 1475, 1564], 'neither': [1406], 'close': [1407], 'enough': [1408], 'Tyr-319': [1410, 1497], 'interact': [1412], 'directly': [1413], 'nor': [1431, 4154], 'does': [1432, 1579, 4325], 'make': [1434], 'direct': [1435], 'contacts': [1436], 'nucleotides': [1438], '15.Changela': [1463], 'affect': [1486, 3126, 3497], 'indirectly': [1489], 'change': [1493, 3034, 3492], 'conformation': [1494], 'approach': [1496], 'In': [1515, 3508, 3638, 3840, 4003, 4322], 'E.': [1519, 1935, 2035, 2289, 2396, 2408, 2652, 2873, 3053, 3285, 3317, 3757, 3942, 4485], 'colitopoisomerase': [1520, 3943], 'complexed': [1522], 'part': [1529, 1668], 'hydrogen-bonding': [1532], 'donate': [1539], 'Asp-111': [1543], 'then': [1546, 2200, 2673, 3474, 3518], 'relay': [1547], 'Glu-9': [1551, 1666], '2).': [1568], 'Mutagenesis': [1569], 'shown': [1572, 3900], 'changing': [1574, 3519], 'overall': [1582, 4259], 'enzymatic': [1583, 3636, 3724, 3764, 4418], 'mutation': [1607, 2030], 'results': [1608, 3630], 'level': [1612], 'makes': [1617], 'excellent': [1621], 'candidate': [1622], 'in-depth': [1625], 'study.': [1626], 'To': [1627, 2777, 3426, 4110, 4611], 'determine': [1628, 1720, 1771, 2778, 3223, 3955, 4231, 4612], 'whether': [1629, 3967, 4613], '(a)': [1631], 'acts': [1632, 3469], 'abstract': [1638], 'Tyr-319;': [1647], '(b)': [1648], 'protonates': [1649], '3′-OH': [1651, 3391], 'leaving': [1654, 3394], 'acid;': [1660], '(c)': [1661], 'donates': [1662, 3386], 'network;': [1672], '(d)': [1674], 'binding,': [1678, 4562], 'glutamine,': [1686, 3039], 'aspartate.': [1690, 3043], 'effect': [1692, 1723], 'concentration': [1697, 2069, 2137, 2247, 3683, 3806, 3860, 4086, 4131, 4139, 4160, 4198, 4228], 'examined.': [1703], 'compared': [1712, 4422], 'values': [1716, 4474], 'order': [1718], 'relative': [1722], '(16.Roche': [1727, 3902], 'Y.': [1730, 3905], 'Int.': [1731, 3906], 'Macromol.': [1734, 3909], '175-180Crossref': [1737, 3912], '(3)': [1740, 3915], 'under': [1747, 3971, 4011], 'steady-state': [1748], 'conditions': [1749, 4012], 'assessed': [1751], 'using': [1752, 2001, 2186, 2210, 2400, 2524, 2998], 'polarization.': [1754], 'Fluorescence': [1755, 2928], 'used': [1759, 1916, 1965, 1977, 1986, 2430, 3032, 4023, 4229, 4644], 'investigate': [1761, 3792], 'concentrations': [1768, 3691, 3772], 'number': [1773], 'ionic': [1775], 'charges': [1776], 'displaced': [1777], 'binding.': [1782], 'amplified': [1787, 1840], 'plasmid': [1789, 1813, 1862, 1865, 1926, 2042, 2296, 3071], 'pJW312': [1790], '(17.Zumstein': [1791], '333-340Crossref': [1800], '(62)': [1803], 'PCR': [1807], 'inserted': [1809, 1843], 'pET15b': [1812, 1851], 'Novagen.': [1815], 'unique': [1817, 1829, 2013], 'NdeI': [1818, 1854], 'restriction': [1819, 1831, 2014], 'introduced': [1822, 1834], 'primer': [1825, 1837], '5′-GGACCATAGCATATGGGTAAAGCTCTTGTC-3′,': [1826], 'XhoI': [1830, 1858], '5′-CCGCTCGAGTCATTTTTTTCCTTC-3′.': [1838], 'in-frame': [1844], 'multiple': [1847], 'cloning': [1848], 'create': [1861], 'pHis97.': [1863], 'encodes': [1866], 'entire': [1868], 'addition': [1874, 2129, 2240, 2381, 2677], '6-residue': [1877], 'tag': [1879], 'linker': [1882], 'region': [1883, 3580], 'containing': [1884, 2039, 2293, 2816, 2840], 'thrombin': [1886], 'N': [1891], 'native': [1895], 'protein.': [1896, 2747], 'accuracy': [1898], 'subcloning': [1901], 'confirmed': [1903], 'sequencing.': [1906], 'Unique': [1908], 'Site': [1909], 'Elimination': [1910], 'kit': [1911, 2006], 'Amersham': [1913], 'Biosciences': [1914], 'construction': [1919, 1983], 'pT67,': [1927], 'fused': [1940], '(GST)1': [1944], '(18.Lima': [1945], '1993;': [1954], '232:': [1955], '1213-1216Crossref': [1956], '(43)': [1959], 'determination': [1968], 'assays.': [1973, 1992, 2435], 'Plasmid': [1974, 2391], 'pHis97': [1975], 'template': [1980], 'Full-length': [1993], 'histidine-tagged': [1997, 3108], 'generated': [2000], 'QuikChange': [2003], 'site-directed': [2004], 'Stratagene.': [2008], 'Each': [2009, 2713], 'contained': [2011, 2569, 2609, 2717, 3072], 'location': [2018], 'interest.': [2020], 'Mutant': [2021], 'identity': [2022], 'verified': [2024], 'sequencing': [2027, 3066], 'Ten': [2032], 'liters': [2033], 'DH5α': [2037, 2398], 'cells': [2038, 2292, 2312, 2399], 'grown': [2044, 2300], '34': [2046], '°C': [2047, 2686], 'BioFlo': [2050], 'IV': [2051], 'fermentor': [2052], '(New': [2053], 'Brunswick': [2054], 'Scientific': [2055], 'Co.).': [2056], 'Expression': [2057, 3044], 'induced': [2062], 'adding': [2064, 3616], 'isopropyl-1-thio-β-d-galactopyranoside': [2065], 'final': [2068, 2136, 2179, 2246, 2659, 3012], '0.3': [2071, 2516], 'mm.': [2072, 2250], 'Cells': [2073, 2335, 2347], 'lysed': [2075, 2349], 'modified': [2078], 'Brij/lysozyme': [2079], 'procedure': [2080], '(19.Godson': [2081], 'G.N.': [2082], 'Sinsheimer': [2083], 'R.L.': [2084], 'Biochim.': [2085], 'Acta.': [2087], '1967;': [2088], '149:': [2089], '476-488Crossref': [2090], '(121)': [2093], 'incubation': [2097], '20%': [2099], 'sucrose,': [2100], '50': [2101], 'mm': [2102, 2105, 2117, 2140, 2220, 2387, 2507, 2517, 2553, 2561, 2576, 2616, 2640, 2738, 2741, 2842, 2857, 3643, 3669, 3717, 3737, 3741, 3753, 3824], 'Tris,': [2103], '25': [2104], 'EDTA,': [2106], '100': [2107, 3642], 'mmβ-mercaptoethanol,': [2108], '2.5': [2109, 2112], 'μg/ml': [2110, 2113], 'leupeptin,': [2111], 'pepstatin': [2114], 'A,': [2115], '0.625': [2116], 'phenylmethylsulfonyl': [2118, 2242], 'fluoride,': [2119], '0.25': [2121], 'mg/ml': [2122], 'lysozyme': [2123], '15': [2125, 2229, 2688], 'min.': [2126, 2689], 'followed': [2127], 'KCl': [2131, 2588, 2742, 3738], 'Brij-58': [2133], '120': [2139, 3668, 3740, 3752], '0.25%,': [2142], 'respectively.': [2143], 'separated': [2147, 2694, 2808], 'membranes': [2149], 'ultracentrifugation': [2154], '113,613': [2156], '×': [2157, 2963], 'g': [2158], '(40,000': [2159], 'rpm': [2160], 'Beckman': [2163], '60': [2164, 3736, 3823], 'Ti': [2165], 'fixed': [2166], 'angle': [2167], 'rotor).': [2168], 'Additional': [2169], 'removed': [2172], 'polymin': [2174, 2364], 'P': [2175, 2365], 'precipitation': [2176, 2366], '(1%': [2177], 'w/v': [2178], 'concentration).': [2180], 'fusion': [2182, 2202, 2357], 'purified': [2185, 2255, 2394, 2535, 2547, 3101], 'GS-Sepharose': [2188, 2271], '(Amersham': [2189, 2196], 'Biosciences)': [2190, 2197], 'column': [2192, 2273, 2283, 2375], 'Sephacryl': [2194, 2281], 'S-100': [2195, 2282], 'column,': [2198], 'remove': [2207, 2285], 'GST': [2209, 2263, 2356, 3063], '0.9': [2211], 'NIH': [2212], 'units': [2213, 2980], 'thrombin/mg': [2215], '150': [2219], 'NaCl,': [2221], '16': [2222], 'mmNa2HPO4,': [2223], '4': [2224], 'mmNaH2PO4': [2225], '(pH': [2226, 2389, 2509, 2519, 2555, 2578, 2732, 2844], '7.3)': [2227], 'h': [2230], 'room': [2232, 2668, 2926, 2940], 'temperature.': [2233, 2927, 2941], 'stopped': [2238, 2674], 'fluoride': [2243], '1': [2249, 2506, 2552, 2671, 2718, 2800, 2997, 4135], 'away': [2256, 4041], 'non-cleaved': [2259], 'free': [2262], 'second': [2270], 'applying': [2275], 'flow-through': [2277], 'fraction': [2278, 4401, 4407], 'residual': [2287], 'thrombin.': [2288], 'BL21': [2291], 'interest': [2298], '37': [2302], '°C.': [2303, 2346], 'Protein': [2304, 2368], 'expression': [2305], 'initiated': [2307], 'infection': [2309], 'bacteriophage': [2314], 'λ': [2315], 'CE6': [2316], '(20.Studier': [2317], 'F.W.': [2318], 'Rosenberg': [2319], 'A.H.': [2320], 'Dunn': [2321], 'Dubendorff': [2323], 'J.W.': [2324], 'Methods': [2325], 'Enzymol.': [2326], '1990;': [2327], '185:': [2328], '60-89Crossref': [2329], '(6011)': [2332], 'harvested,': [2337], 'frozen': [2338], 'liquid': [2340], 'nitrogen,': [2341], 'stored': [2343, 2550], '−70': [2345], 'same': [2352, 3118, 3650, 3932, 4447], 'proteins': [2358, 3112], 'without': [2359], 'including': [2360], 'EDTA': [2361], 'step.': [2367], 'nickel-nitrilotriacetic': [2373], 'acid-agarose': [2374], '(Qiagen)': [2376], 'eluted': [2378, 2504], 'stepwise': [2380], '50,': [2383], '500,': [2384], '750': [2386], 'imidazole': [2388, 3478, 3618, 3658], '8).': [2390], 'pBR322': [2392], 'cesium': [2402], 'chloride': [2403], 'gradient': [2404], '(21.Sambrook': [2405], 'Fritsch': [2407], 'Maniatis': [2409], 'T.': [2410], 'Molecular': [2411], 'Cloning:': [2412], 'Laboratory': [2414, 2421], 'Manual.': [2415], '2nd': [2416], 'Ed.': [2417], 'Cold': [2418, 2423], 'Spring': [2419, 2424], 'Harbor': [2420], 'Press,': [2422], 'Harbor,': [2425], 'NY1989:': [2426], '1.38-1.46Google': [2427], 'Fluorescein-labeled': [2436], 'oligonucleotides': [2437, 2533, 3876], 'assays': [2442, 3664, 3687], 'iodinated': [2445, 2728], 'assay': [2450, 2749, 3953, 4172], 'purchased': [2452, 2489], 'Sigma.': [2454], 'Oligonucleotides': [2455], 'labeled': [2457, 3884], 'described': [2459, 2753], 'previously': [2460, 2754, 3901], '(22.Zhang': [2461, 2879], 'H.L.': [2462, 2880], 'Malpure': [2463, 2881], 'S.': [2464, 2882, 3987], 'Li': [2465, 2883], 'Z.': [2466, 2884], 'Hiasa': [2467, 2885], '271:': [2475, 2893], '9039-9045Abstract': [2476, 2894], '(27)': [2484, 2902], '[γ-32P]ATP': [2488], 'PerkinElmer': [2491], 'Life': [2492], 'Sciences,': [2493], 'except': [2494], 'radiolabeled': [2497], 'excised': [2501], 'Tris-HCl': [2508, 2554, 2577, 2624, 2843], '8.0': [2510, 2556, 2579, 2733, 2845], '22': [2512, 2522, 2558, 2581, 2735, 2847], '°C),': [2513, 2559, 2582, 2736, 2848], '0.1': [2514, 2560], 'mmEDTA,': [2515], 'NaOAc': [2518], '5.0': [2520], '°C)': [2523], 'microfilterfuge': [2525], 'tubes': [2526], 'Rainin': [2528], 'Instrument': [2529], 'Co.,': [2530], 'Inc.': [2531], 'phenol:chloroform:isoamyl': [2537], 'alcohol': [2538], '(25:24:1)': [2539], 'extraction': [2540], 'subsequently': [2542], 'precipitated': [2543], 'ethanol.': [2545], 'EDTA.': [2562], 'Relaxation': [2563, 2603, 3164], 'mixtures': [2564, 2604], '400': [2570, 2610], 'ng': [2571, 2611, 2643, 2649], 'pBR322,': [2574, 2614], '40': [2575, 2615, 2730, 2841], '5': [2583, 2637, 2639, 2737, 2740, 2849, 2962], 'mmMgCl2,': [2584, 2638, 2850], 'amount': [2586, 2593, 2919], 'indicated,': [2589], 'indicated': [2592, 2918], 'total': [2598, 2907], 'volume': [2599, 2660, 2908], '20': [2601, 2642, 2662], 'μl.': [2602, 2663], 'profiles': [2608], 'buffer': [2617], '(MES': [2618], '5.5': [2621, 3154], '6.5,': [2623], '7.0': [2627], '8.5,': [2629], 'CHES': [2631], '9.0': [2634], '10.5),': [2636], 'KCl,': [2641, 2858, 3670, 3742, 3825], '500': [2648, 2744, 2910, 4187, 4224], 'Reactions': [2664, 2839], 'incubated': [2666], 'temperature': [2669], 'h,': [2672, 4299], '2%': [2679], '(w/v)': [2680, 2698, 2818], 'SDS,': [2681], 'heat-inactivated': [2683], '65': [2685], 'products': [2692, 2806], '1%': [2697], 'agarose': [2699], 'gel': [2700, 2815], '(1×': [2701], 'TAE': [2702], 'buffer),': [2703], 'stained': [2704], 'ethidium': [2706], 'bromide,': [2707], 'visualized': [2709, 2827], 'ultraviolet': [2711], 'illumination.': [2712], '30-μl': [2714], 'mixture': [2716], 'μm': [2719], '5′-radiolabeled': [2720], 'sequence': [2724, 2865], '5′-UAAAAAAA-3′': [2725], 'uracil,': [2729], 'mmTris-HCl': [2731], 'MgCl2,': [2739], 'ng/μl': [2745, 4188, 4225], '(23.Zhu': [2755, 3171], '1997;': [2764, 3180], '272:': [2765, 3181], '16206-16210Abstract': [2766, 3182], '(44)': [2774, 3190], 'time': [2783], 'points': [2784], 'taken': [2786, 2933, 2945], '30': [2788, 2797], 's;': [2789], '1,': [2790, 4005, 4143], '2,': [2791, 4144], '5,': [2792, 2851], '10,': [2793, 3218], '15,': [2794], '20,': [2795, 2852], 'min;': [2798], '2': [2802, 2859, 4298, 4396], 'h.': [2803], 'electrophoresis': [2810], '19:1': [2813], 'polyacrylamide': [2814], '50%': [2817], 'urea': [2819], '1×': [2821], 'Tris': [2822], 'borate-EDTA': [2823], 'buffer.': [2824], 'Gels': [2825], 'autoradiography': [2829], 'Packard': [2832], 'InstantImager.': [2833], 'Assays': [2834], 'done': [2836, 3950], 'triplicate.': [2838], '40,': [2853], '60,': [2854], '80': [2856], 'nm': [2860, 2952, 2959], '5′-fluorescein-labeled': [2861], '5′-CGC↓AAC↓TT-3′': [2866], '(where': [2867], '↓': [2868], 'indicates': [2869], 'Scholar))': [2904], 'μl': [2911], 'allowed': [2913], 'equilibrate': [2915], '3': [2923], 'min': [2924], '(FP)': [2930], 'measurements': [2931, 2943, 4642], 'ISS': [2936], 'PC1': [2937], 'Spectrofluorometer': [2938], 'excitation': [2948], 'wavelength': [2949, 2956], '494': [2951], 'emission': [2955], '520': [2958], '10-mm': [2964], 'quartz': [2965], 'cuvette.': [2966], 'Samples': [2967], 'read': [2969], 'until': [2970], 'standard': [2972, 4329], 'deviation': [2973, 4330], 'each': [2975], 'measurement': [2976], '10': [2978, 3160, 3253], 'mP': [2979], 'below.': [2982], 'Assuming': [2983, 4381], '1:1': [2985], 'stoichiometry,': [2986], 'FP': [2987], 'isotherms': [2989], 'fit': [2991], 'logistical': [2994], 'Equation': [2995], '1,Y1−Y21+(x/x0)P+Y2Equation': [2996], 'Origin': [2999], 'software': [3000], 'package': [3001], '(Microcal),': [3002], 'where': [3003, 4397], 'Y1': [3004], 'initial': [3007], 'Y': [3008, 3013], 'value;Y2': [3009], 'value;x0': [3014], 'constant;': [3018], 'p': [3020], 'power,': [3023], 'set': [3026], '1.': [3028], 'Site-directed': [3029], 'full-length': [3050], 'version': [3051], 'fusion.': [3064], 'data': [3067, 4390], 'showed': [3068], 'correct': [3074], 'sequence.': [3075], 'However,': [3076, 4180, 4222], 'induction': [3077], 'resulted': [3078], 'degraded': [3081], '(data': [3086, 3104, 3128, 3161, 3254, 3621, 3659], 'shown).': [3088, 3106, 3130, 3163, 3623, 3661], 'Instead,': [3089], 'made': [3093, 3453, 3964], 'polyhistidine-tagged': [3096], 'construct.': [3097], 'homogeneity': [3103], 'extent,': [3119], 'indicating': [3120], 'histidines': [3123], 'maximal': [3136, 3529, 3542, 3590, 3723, 4090, 4164, 4238, 4313, 4354, 4406], '8': [3140], 'broad,': [3143], 'bell-shaped': [3144, 3552], 'curve.': [3146], 'Activity': [3147], 'ceases': [3148], 'detectable': [3151, 3236], 'above': [3158, 3229, 3545, 3751], 'negatively': [3166, 3291, 3709, 3855], 'requires': [3169], 'magnesium': [3170, 3209, 3272], '24.Domanico': [3193], 'P.L.': [3194], 'Inorg.': [3198], '1991;': [3200], '42:': [3201], '87-96Crossref': [3202], '(45)': [3205], 'As': [3208, 4570], 'begins': [3210], 'precipitate': [3212], 'out': [3213], 'solution': [3215], 'impossible': [3221], 'accurately': [3224], '10.': [3231], 'there': [3233], 'no': [3235], '10.5': [3246], 'minimal': [3248, 3557, 3732, 4295], 'shown),': [3256], 'unclear': [3259], 'if': [3260], 'this': [3261, 3681, 3952, 4058], 'lack': [3262], 'loss': [3270, 4620, 4633], 'below': [3273, 3565], 'threshold': [3275], 'needed': [3276], 'occur.': [3280], 'relaxing': [3290], 'suggests': [3294, 4202], 'undergoes': [3298], 'cysteine': [3309, 3328], 'involved.': [3312], 'sites': [3315], '13.Mondragón': [3344], 'Scholar);': [3362], '365.': [3371], 'strand;': [3396], 'abstracts': [3402], 'tyrosine;': [3409], 'donating': [3413], 'acid,': [3420, 3473, 3517], 'network.': [3425], 'test': [3427, 3455], 'base,': [3438], 'arginine.': [3447], 'Polar': [3448], 'acidic': [3450, 3524, 3571], 'substitutions': [3451, 3572], 'acid.': [3466], 'If': [3467], 'deprotonation': [3475], 'ring': [3479], 'will': [3480, 3526], 'increase': [3481], 'should': [3495, 3510, 4104], 'polar': [3503, 3569], 'location.': [3507], 'addition,': [3509], 'side-chain': [3512], 'shift': [3527, 3540], 'show': [3538, 3688, 3810], '8.5': [3547], 'sharper,': [3549], 'attenuated': [3551], 'curves': [3553], '3).': [3555], 'Only': [3556], 'exhibited': [3561], '7.': [3567], 'shifted': [3573], 'basic': [3579], 'slightly': [3581], 'arginine': [3587], '9.0–9.5.': [3594], 'No': [3595], 'increased': [3596, 4141], 'observed': [3602, 4061, 4251, 4412], 'any': [3604, 4255], 'mutant.': [3605, 4277], 'Attempts': [3606], 'regain': [3608], 'type-like': [3610], 'exogenous': [3617], 'unsuccessful': [3620], 'Addition': [3624], 'up': [3626, 4185], '1m': [3628], 'imidizole': [3629], 'decrease': [3633], 'imidazole,': [3644], 'profile': [3652], 'absence': [3656, 3728, 3829], 'Originally,': [3662], 'fully': [3676], '4).': [3685, 3778, 3863], 'increasing': [3690, 4196], 'inhibit': [3694], 'enzymes.': [3702], '0–140': [3716], 'KCl.': [3718, 3754], 'salt,': [3730, 3831], 'lose': [3744], 'Wild': [3755], 'full': [3763], 'over': [3766, 4016], 'range': [3769], 'Successive': [3779], '5-fold': [3780], 'dilutions': [3781, 3809], 'degree': [3794], 'outperforms': [3801], 'increases.': [3807], 'Serial': [3808], '∼125-fold': [3815], 'able': [3817, 3848], '∼25-fold': [3837], 'active.': [3839], 'cases,': [3842], 'increases': [3861], '8-base': [3881], 'oligomer': [3882], 'radioactively': [3883], '5′': [3887], 'Polydeoxyadenosine': [3889], 'iodo-uracil': [3892, 3927], 'first': [3895], '(diUA7)': [3897], 'relatively': [3921], 'fast': [3922], 'rate,': [3924, 4413], 'maintains': [3928], 'oligonucleotide.': [3936], 'Given': [3937, 4327], 'definitive': [3939], '(TopoI)': [3945], 'kinetics': [3957], 'reaction,': [3960], 'attempt': [3962], 'assess': [3966], 'occurred': [3970], 'turnover': [3973], 'conditions,': [3974], 'Scheme': [3978, 4004], 'presented': [3979], 'Stivers': [3981], 'et': [3982], 'al.': [3983], '(25.Stivers': [3984], 'J.T.': [3985], 'Shuman': [3986], 'Mildvan': [3988], 'A.S.': [3989], '33:': [3992], '327-339Crossref': [3993], '(95)': [3996], 'vaccinia': [4000], 'I.konkCldiUA7·+TopoI⇌diUA7·/TopoI⇀diUA3·+A4+TopoIkoff': [4002], 'irreversible': [4008], 'excess': [4015], 'length': [4019], 'prevents': [4024], 'occurring': [4027], 'would': [4039, 4335], 'diffuse': [4040], '(26.Tse-Dinh': [4042], '261:': [4048], '10931-10935Abstract': [4049], 'Therefore,': [4056], 'scheme,': [4059], 'principle': [4065], 'reflect': [4067, 4093], 'step': [4071, 4075, 4103], 'reaction.': [4078, 4380], 'Observed': [4079], 'independent': [4083], 'reach': [4088], 'likely': [4092], 'first-order': [4094, 4386], 'steps': [4096], 'mechanism,': [4099], 'dependent': [4106, 4500], 'concentration.': [4109, 4221], 'maintain': [4111], 'limiting': [4112], 'amounts': [4113], 'oligonucleotide,': [4115], 'DNA:protein': [4116], 'ratios': [4117], '1:1,': [4119], '1:2,': [4120], '1:4,': [4121], '1:5,': [4122], '1:8': [4124], '8.': [4129, 4321], 'maintained': [4133], 'μm,': [4136], '3.6,': [4145], '5.1,': [4146], '7.7': [4148], 'μm.': [4149], 'Neither': [4150], 'appear': [4157, 4336], 'regarding': [4162], 'extent': [4165, 4245, 4260, 4314, 4345], 'reports': [4173], 'inseparably.': [4179], 'heavily': [4183], 'concentration-dependent': [4184, 4208], '(5.1': [4189], 'μm)': [4190], 'decreasing': [4194], '5).': [4200], 'inactivation': [4209], 'due,': [4213], 'aggregation': [4218], 'high': [4220], '(the': [4226], 'dependence)': [4233], 'reached': [4236, 4311], 'rate.': [4240], '6).': [4253, 4364], 'At': [4254], 'value,': [4257], '6,': [4289], 'After': [4297, 4561], 'appears': [4301], '7': [4319, 4440], 'contrast,': [4323], 'not.': [4326], 'three': [4332], 'experiments,': [4333], '7–9': [4350], 'reaches': [4352], 'rapidly': [4357], 'better': [4366], 'way': [4367], 'compare': [4369], 'activities': [4372], 'examine': [4375], 'fitting': [4388], 'exponential': [4394], '(Equation2),%Cl=%Cl0·(1−e−k·t)Equation': [4395], '%Cl': [4398], 'cleaved,': [4402, 4408], '%Cl0': [4403], 'k': [4409], 't': [4415], 'time,': [4417], 'values.': [4426], 'seen': [4430], '8,': [4442], 'follows': [4445], 'relaxation.': [4451], 'lacks': [4459], '7).Figure': [4463], '7Comparison': [4464], 'versus': [4472], '(▪),': [4478], '(▴),': [4480], '(●)': [4483], 'coliDNA': [4486], 'contrast': [4504], '(fraction': [4512], 'per': [4516], 'min)': [4517], 'plotted': [4519], 'function': [4522], 'pH.View': [4524], 'Large': [4525], 'Image': [4526], 'Figure': [4527], 'ViewerDownload': [4528], 'Hi-res': [4529], 'image': [4530], 'Download': [4531], '(PPT)': [4532], 'action': [4536, 4598], 'closed': [4543], 'must': [4545, 4575], 'open': [4546], 'groove': [4555], 'leading': [4556], 'occur': [4576], 'measuring': [4579], 'clearer': [4592], 'picture': [4593], 'various': [4603], 'cleavage.': [4610], 'sensitivity': [4616], 'result': [4630], 'gain': [4635], 'ability,': [4639], 'look': [4646], 'technique': [4652]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2045397094', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 2}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-09T23:48:57.765927', 'created_date': '2016-06-24'}