Title: Glutamate dehydrogenase and ammonia production in the eel (Anguilla rostrata)
Abstract: 1. Glutamate dehydrogenase (GDH; EC. 1.4.1.3) activity was studied in homogenates of tissues from the fresh-water eel (Anguilla rostrata) with a monometric technique. Optimal conditions were determined for the assay of GDH in liver homogenates. Under these conditions, kidney showed a GDH activity about half that of liver, gill activity was low and that of muscle was negligible. 2. In eel liver homogenates, under optimal assay conditions, the Vmax (as QO2 n a wet weight basis) for the deamination of glutamate was 3·45. The Km (glutamate) was 1·55 × 10−2 M. Ammonia inhibited the reactoon competitively, with a Ki of 1·15 × 10t−2 M. 3. Transdeamination of alanine via glutamate appeared to occur at a significant rate in eel liver. 4. Significant liver GDH activity was also found in two marine teleost species and two elasmobranchs.
Publication Year: 1966
Publication Date: 1966-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 37
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot