Title: Solid State NMR Investigation of the Interaction between Biomimetic Lipid Bilayers and de novo Designed Fusogenic Peptides
Abstract: ChemBioChemVolume 8, Issue 5 p. 493-496 Communication Solid State NMR Investigation of the Interaction between Biomimetic Lipid Bilayers and de novo Designed Fusogenic Peptides Prashant Agrawal, Prashant Agrawal Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorSuzanne Kiihne Dr., Suzanne Kiihne Dr. Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorJohan Hollander, Johan Hollander Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorFrans Hulsbergen Dr., Frans Hulsbergen Dr. Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorMathias Hofmann Dr., Mathias Hofmann Dr. Lehrstuhl Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, GermanySearch for more papers by this authorDieter Langosch Prof., Dieter Langosch Prof. Lehrstuhl Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, GermanySearch for more papers by this authorHuub de Groot Prof., Huub de Groot Prof. [email protected] Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this author Prashant Agrawal, Prashant Agrawal Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorSuzanne Kiihne Dr., Suzanne Kiihne Dr. Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorJohan Hollander, Johan Hollander Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorFrans Hulsbergen Dr., Frans Hulsbergen Dr. Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this authorMathias Hofmann Dr., Mathias Hofmann Dr. Lehrstuhl Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, GermanySearch for more papers by this authorDieter Langosch Prof., Dieter Langosch Prof. Lehrstuhl Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, GermanySearch for more papers by this authorHuub de Groot Prof., Huub de Groot Prof. [email protected] Biophysical Organic Chemistry/Solid State NMR, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands, Fax: (+31) 71-5274603Search for more papers by this author First published: 13 March 2007 https://doi.org/10.1002/cbic.200600518Citations: 10Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Learning curve. We have studied the interaction of two de novo designed hydrophobic peptides with a biomimetic three-lipid mixture and pure POPC bilayer by using 31P solid-state NMR spectroscopy. The observed peptide-induced phase changes correlated with the fusogenic behaviour of the peptides in liposomes. The peptides induced pronounced stabilization of curvature at low humidity (see figure). We propose that this stabilization might be a prerequisite for the fusogenic behaviour of the peptides. References 1M. Hofmann, K. Weise, J. Ollesch, P. Agrawal, H. Stalz, W. Stelzer, F. Hulsbergen, H. de Groot, K. Gerwert, J. Reed, D. Langosch, Proc. Natl. Acad. Sci. USA 2004, 101, 14776– 14781. 2J. J. Buffy, M. J. McCormick, S. Wi, A. Waring, R. I. Lehrer, M. Hong, Biochemistry 2004, 43, 9800– 9812. 3K. J. Hallock, D. K. Lee, A. Ramamoorthy, Biophys. J. 2003, 84, 3052– 3060. 4J. W. Deutsch, R. B. Kelly, Biochemistry 1981, 20, 378– 385. 5K. A. H. 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