Title: Effect of ferulate on migration induced by endothelin and platelet derived growth factor in cultured vascular smooth muscle cells from shr
Abstract: This study was to investigate the effect of sodium ferulate (SF), one of the principal components of chinese rhizoma ligustici wallichi, on the migration induced by endothelin and platelet derived growth factor (PDGF) in vascular smooth muscle cells. Cultured vascular smooth muscle cells derived from spontaneously hypertensive rats(SHR) were used. Cell migration was determined by modified Boyden chamber assays. Intracellular free calcium([Ca2+]i) was measured with fluorescent [Ca2+]i indicator Fura-2/AM. It was showed that endothelin and PDGF significantly induced a migration of vascular smooth muscle cells in a dose-dependent manner, which was dose-dependently inhibited by pre-treatment with 10-7-10-3mol/L sodium ferulate . The peak inhibition rate of migration induced by endothelin and PDGF were 85.04% and 81.92% (P<0.01) respectively. endothelin and PDGF provoked the rise of [Ca2+]i in vascular smooth muscle cells, which was significantly suppressed by 10-3mol/L sodium ferulate (P<0.01). It is concluded that migration and rise of [Ca2+]i induced by endothelin and PDGF in vascular smooth muscle cells from SHR may be suppressed by ferulate. Am J Hypertens (2004) 17, 173A–174A; doi: 10.1016/j.amjhyper.2004.03.457