Title: Eosinophil granules and phosphotungstic acid: An electron microscope study of guinea-pig bone marrow
Abstract: The granules of bone marrow eosinophils have been studied by means of electron microscopy in a series of 15 normal guinea-pigs. Staining with phosphotungstic acid produced a striking change in the appearance of the granules. Whereas in unstained material, the internum (or crystalloid) appeared denser than the surrounding matrix, after phosphotungstic acid staining, the internum appeared relatively electron transparent in contrast to the dense matrix. Granules which showed no internum appeared to have a uniform density in unstained material, but after phosphotungstic acid staining, some granules showed a dense peripheral area surrounding a central area of relative electron-transparency. These findings were obtained both after staining of the tissue with various solutions of phosphotungstic acid during dehydration, and also after staining of sections with solutions of phosphotungstic acid in absolute alcohol on the grid. Similar results were found after staining with phosphomolybdic acid. It is suggested that the results may help to throw light on the nature and development of components of the eosinophil granule.
Publication Year: 1966
Publication Date: 1966-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 29
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot