Title: [<sup>3</sup>H]Neurotensin(8–13) Binds in Human Brain to the Same Sites as Does [<sup>3</sup>H]Neurotensin but with Higher Affinity
Abstract: Abstract: The binding of [ 3 H]neurotensin(8–13) to membranes from human frontal cortex at 0°C was time dependent, specific, saturable, and reversible. Saturation isotherms provided an equilibrium dissociation constant ( K D ) of 0.52 n M , and the maximal number of binding sites ( B max) was 3.5 pmol/g original wet weight of tissue. Scat‐chard analysis yielded a straight line, and the Hill coefficient was equal to 1, a result indicating that [ 3 H]‐neurotensin(8–13) bound to single, noncooperative sites. The K D values of several analogs of neurotensin determined in competition with [ 3 H]neurotensin(8–13) were similar to those previously determined in competition with [ 3 H]‐neurotensin. The regional distribution of binding sites for [ 3 H]neurotensin(8–13) was also similar to that for [ 3 H]‐neurotensin. These results suggest that [ 3 H]neurotensin(8–13) binds to the same sites as [ 3 H]neurotensin and that [ 3 H]neurotensin(8–13) has a higher affinity than [ 3 H]‐neurotensin for these sites in human brain.
Publication Year: 1988
Publication Date: 1988-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 37
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