Publication Information

{'id': 'https://openalex.org/W2036773544', 'doi': 'https://doi.org/10.1074/jbc.m404778200', 'title': 'Ca2+-selective Transient Receptor Potential V Channel Architecture and Function Require a Specific Ankyrin Repeat', 'display_name': 'Ca2+-selective Transient Receptor Potential V Channel Architecture and Function Require a Specific Ankyrin Repeat', 'publication_year': 2004, 'publication_date': '2004-06-15', 'ids': {'openalex': 'https://openalex.org/W2036773544', 'doi': 'https://doi.org/10.1074/jbc.m404778200', 'mag': '2036773544', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15192090'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m404778200', 'pdf_url': 'http://www.jbc.org/article/S0021925820772765/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925820772765/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5044429656', 'display_name': 'Isabell Erler', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I91712215', 'display_name': 'Saarland University', 'ror': 'https://ror.org/01jdpyv68', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I91712215']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Isabell Erler', 'raw_affiliation_strings': ['Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany', 'institution_ids': ['https://openalex.org/I91712215']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5071306975', 'display_name': 'Daniela Hirnet', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I91712215', 'display_name': 'Saarland University', 'ror': 'https://ror.org/01jdpyv68', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I91712215']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Daniela Hirnet', 'raw_affiliation_strings': ['Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany', 'institution_ids': ['https://openalex.org/I91712215']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5068381952', 'display_name': 'Ulrich Wissenbach', 'orcid': 'https://orcid.org/0000-0003-2665-3782'}, 'institutions': [{'id': 'https://openalex.org/I91712215', 'display_name': 'Saarland University', 'ror': 'https://ror.org/01jdpyv68', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I91712215']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Ulrich Wissenbach', 'raw_affiliation_strings': ['Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany', 'institution_ids': ['https://openalex.org/I91712215']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5056752653', 'display_name': 'Veit Flockerzi', 'orcid': 'https://orcid.org/0000-0001-6002-6294'}, 'institutions': [{'id': 'https://openalex.org/I91712215', 'display_name': 'Saarland University', 'ror': 'https://ror.org/01jdpyv68', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I91712215']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Veit Flockerzi', 'raw_affiliation_strings': ['Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany', 'institution_ids': ['https://openalex.org/I91712215']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5018232279', 'display_name': 'Barbara A. Niemeyer', 'orcid': 'https://orcid.org/0000-0002-6963-0575'}, 'institutions': [{'id': 'https://openalex.org/I91712215', 'display_name': 'Saarland University', 'ror': 'https://ror.org/01jdpyv68', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I91712215']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Barbara A. Niemeyer', 'raw_affiliation_strings': ['Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Experimentelle und Klinische Pharmakologie und Toxikologie der Universität des Saarlandes, 66421 Homburg, Germany', 'institution_ids': ['https://openalex.org/I91712215']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 6.066, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 142, 'citation_normalized_percentile': {'value': 0.915307, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 97, 'max': 98}, 'biblio': {'volume': '279', 'issue': '33', 'first_page': '34456', 'last_page': '34463'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11204', 'display_name': 'Ion Channels and Receptors', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2809', 'display_name': 'Sensory Systems'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11204', 'display_name': 'Ion Channels and Receptors', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2809', 'display_name': 'Sensory Systems'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10493', 'display_name': 'Ion channel regulation and function', 'score': 0.9648, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T13483', 'display_name': 'Herbal Medicine Research Studies', 'score': 0.9486, 'subfield': {'id': 'https://openalex.org/subfields/2707', 'display_name': 'Complementary and alternative medicine'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/trpv', 'display_name': 'TRPV', 'score': 0.9347209}, {'id': 'https://openalex.org/keywords/ankyrin-repeat', 'display_name': 'Ankyrin repeat', 'score': 0.8940212}, {'id': 'https://openalex.org/keywords/trpv6', 'display_name': 'TRPV6', 'score': 0.85524535}, {'id': 'https://openalex.org/keywords/ankyrin', 'display_name': 'Ankyrin', 'score': 0.82083297}, {'id': 'https://openalex.org/keywords/immunoprecipitation', 'display_name': 'Immunoprecipitation', 'score': 0.49224415}, {'id': 'https://openalex.org/keywords/hek-293-cells', 'display_name': 'HEK 293 cells', 'score': 0.43310693}], 'concepts': [{'id': 'https://openalex.org/C87649029', 'wikidata': 'https://www.wikidata.org/wiki/Q7671491', 'display_name': 'TRPV', 'level': 5, 'score': 0.9347209}, {'id': 'https://openalex.org/C155164980', 'wikidata': 'https://www.wikidata.org/wiki/Q304043', 'display_name': 'Transient receptor potential channel', 'level': 3, 'score': 0.9295345}, {'id': 'https://openalex.org/C113241181', 'wikidata': 'https://www.wikidata.org/wiki/Q24784174', 'display_name': 'Ankyrin repeat', 'level': 3, 'score': 0.8940212}, {'id': 'https://openalex.org/C155520015', 'wikidata': 'https://www.wikidata.org/wiki/Q14891586', 'display_name': 'TRPV6', 'level': 4, 'score': 0.85524535}, {'id': 'https://openalex.org/C182800266', 'wikidata': 'https://www.wikidata.org/wiki/Q414971', 'display_name': 'Ankyrin', 'level': 3, 'score': 0.82083297}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.5369878}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.53607196}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.5136828}, {'id': 'https://openalex.org/C71829478', 'wikidata': 'https://www.wikidata.org/wiki/Q901305', 'display_name': 'Immunoprecipitation', 'level': 3, 'score': 0.49224415}, {'id': 'https://openalex.org/C50254741', 'wikidata': 'https://www.wikidata.org/wiki/Q62536', 'display_name': 'Ion channel', 'level': 3, 'score': 0.460129}, {'id': 'https://openalex.org/C174510640', 'wikidata': 'https://www.wikidata.org/wiki/Q489618', 'display_name': 'HEK 293 cells', 'level': 3, 'score': 0.43310693}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.39811003}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.39625594}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.2566293}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.23218381}, {'id': 'https://openalex.org/C160145004', 'wikidata': 'https://www.wikidata.org/wiki/Q413209', 'display_name': 'TRPV1', 'level': 4, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D017487', 'descriptor_name': 'Ankyrins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D002118', 'descriptor_name': 'Calcium', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D015220', 'descriptor_name': 'Calcium Channels', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D000595', 'descriptor_name': 'Amino Acid Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017487', 'descriptor_name': 'Ankyrins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019921', 'descriptor_name': 'Biotinylation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002118', 'descriptor_name': 'Calcium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015220', 'descriptor_name': 'Calcium Channels', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015220', 'descriptor_name': 'Calcium Channels', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D002460', 'descriptor_name': 'Cell Line', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002499', 'descriptor_name': 'Centrifugation, Density Gradient', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017353', 'descriptor_name': 'Gene Deletion', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005799', 'descriptor_name': 'Genes, Dominant', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007477', 'descriptor_name': 'Ions', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D008856', 'descriptor_name': 'Microscopy, Fluorescence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008954', 'descriptor_name': 'Models, Biological', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008958', 'descriptor_name': 'Models, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009154', 'descriptor_name': 'Mutation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016376', 'descriptor_name': 'Oligonucleotides, Antisense', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016376', 'descriptor_name': 'Oligonucleotides, Antisense', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D018408', 'descriptor_name': 'Patch-Clamp Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011233', 'descriptor_name': 'Precipitin Tests', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017434', 'descriptor_name': 'Protein Structure, Tertiary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D017386', 'descriptor_name': 'Sequence Homology, Amino Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013395', 'descriptor_name': 'Sucrose', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013395', 'descriptor_name': 'Sucrose', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D050916', 'descriptor_name': 'TRPV Cation Channels', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014162', 'descriptor_name': 'Transfection', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020798', 'descriptor_name': 'Two-Hybrid System Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m404778200', 'pdf_url': 'http://www.jbc.org/article/S0021925820772765/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15192090', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m404778200', 'pdf_url': 'http://www.jbc.org/article/S0021925820772765/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 29, 'referenced_works': ['https://openalex.org/W1521070354', 'https://openalex.org/W1957403800', 'https://openalex.org/W1964607504', 'https://openalex.org/W1967156349', 'https://openalex.org/W1969597161', 'https://openalex.org/W1982951731', 'https://openalex.org/W1983951786', 'https://openalex.org/W1996149118', 'https://openalex.org/W2006398626', 'https://openalex.org/W2016354937', 'https://openalex.org/W2021299730', 'https://openalex.org/W2038724757', 'https://openalex.org/W2040664792', 'https://openalex.org/W2059742450', 'https://openalex.org/W2060957725', 'https://openalex.org/W2068543323', 'https://openalex.org/W2078012495', 'https://openalex.org/W2078831817', 'https://openalex.org/W2086749784', 'https://openalex.org/W2090677452', 'https://openalex.org/W2116370973', 'https://openalex.org/W2122037409', 'https://openalex.org/W2125479650', 'https://openalex.org/W2128783914', 'https://openalex.org/W2142529984', 'https://openalex.org/W2153724411', 'https://openalex.org/W2158029606', 'https://openalex.org/W2159587866', 'https://openalex.org/W2161936455'], 'related_works': ['https://openalex.org/W2765255115', 'https://openalex.org/W2736827087', 'https://openalex.org/W264104730', 'https://openalex.org/W2084435232', 'https://openalex.org/W2082759167', 'https://openalex.org/W2081562770', 'https://openalex.org/W2069178910', 'https://openalex.org/W2039884257', 'https://openalex.org/W2009901513', 'https://openalex.org/W1593680573'], 'abstract_inverted_index': {'Transient': [0, 196, 392], 'receptor': [1, 197, 393, 402, 435], 'potential': [2, 198, 394, 1172, 1689], '(TRP)': [3, 199, 395], 'proteins': [4, 200, 819, 1161, 3146, 3889, 3941], 'form': [5, 27, 148, 201, 223, 344, 593, 711, 820, 891, 1115], 'cation-conducting': [6, 202, 821], 'ion': [7, 203, 469, 822, 902, 1189], 'channels': [8, 142, 204, 338, 462, 470, 582, 713, 853, 4072, 4156], 'with': [9, 126, 205, 322, 567, 824, 1241, 1259, 1419, 1629, 1655, 1734, 1753, 1757, 1777, 1824, 1872, 1876, 1900, 1924, 1935, 1966, 2015, 2056, 2062, 2247, 2338, 2438, 2504, 2519, 2542, 2550, 2573, 2892, 3086, 3209, 3267, 3320, 3537, 3570, 3578, 3585, 3700, 3917, 3963, 3968, 3997, 4014, 4043, 4112, 4222, 4234, 4253, 4326, 4377], 'currently': [10, 206], '28': [11, 207], 'known': [12, 208, 876], 'genes': [13, 209], 'encoding': [14, 210, 2252], 'TRP': [15, 211, 481, 486, 552, 689, 817, 1314], 'channel': [16, 212, 818, 1160, 4200], 'monomers': [17, 21, 213, 217], 'in': [18, 56, 214, 252, 490, 509, 898, 927, 1186, 1280, 1360, 1499, 1533, 1798, 1880, 1941, 1978, 2069, 2188, 2292, 2417, 2427, 2457, 2523, 2531, 2622, 2628, 2749, 2817, 2862, 2990, 3102, 3312, 3427, 3502, 3645, 3828, 3863, 3877, 3991, 4096, 4285, 4288, 4318], 'mammals.': [19, 215], 'These': [20, 216, 2610], 'are': [22, 38, 218, 234, 488, 867, 888, 922, 1184, 1289, 1712, 2592, 2858, 2941, 3437, 3909, 3932, 4341], 'thought': [23, 219, 585], 'to': [24, 26, 119, 220, 222, 315, 475, 505, 579, 590, 592, 890, 924, 1201, 1221, 1225, 1238, 1250, 1322, 1464, 1546, 1683, 1932, 1956, 2019, 2112, 2141, 2377, 2618, 2714, 2731, 2746, 2977, 3496, 3521, 3563, 3614, 3704, 3773, 3817, 3849, 3912, 3935, 4100, 4126, 4135, 4146, 4186, 4197, 4205, 4243, 4259, 4315], 'coassemble': [25, 221], 'homo-': [28, 224], 'or': [29, 132, 147, 225, 328, 343, 518, 879, 894, 1122, 1560, 1910, 2040, 2058, 2255, 2264, 2490, 2545, 2922, 3942, 4292], 'heterotetrameric': [30, 226], 'channels,': [31, 227, 690, 911, 1234, 1315], 'but': [32, 228, 2028, 2720, 3042, 3576, 3641, 4107], 'the': [33, 41, 86, 92, 98, 102, 107, 168, 180, 229, 237, 282, 288, 294, 298, 303, 364, 376, 479, 485, 684, 703, 707, 816, 899, 907, 971, 1150, 1179, 1228, 1252, 1268, 1281, 1306, 1310, 1341, 1380, 1385, 1396, 1400, 1445, 1471, 1482, 1493, 1526, 1706, 1744, 1749, 1792, 1795, 1883, 2002, 2013, 2023, 2030, 2036, 2242, 2248, 2265, 2293, 2383, 2447, 2458, 2478, 2588, 2613, 2680, 2723, 2810, 2815, 2818, 2838, 2863, 2875, 2883, 2911, 2945, 2985, 3000, 3011, 3014, 3036, 3047, 3058, 3068, 3081, 3095, 3113, 3118, 3131, 3157, 3163, 3205, 3268, 3284, 3298, 3302, 3347, 3382, 3425, 3441, 3445, 3470, 3473, 3478, 3486, 3489, 3497, 3500, 3507, 3522, 3538, 3546, 3557, 3567, 3571, 3579, 3586, 3602, 3615, 3623, 3642, 3652, 3672, 3680, 3683, 3688, 3754, 3775, 3854, 3864, 3874, 3878, 3886, 3901, 3927, 3951, 4006, 4025, 4041, 4050, 4056, 4065, 4076, 4087, 4101, 4118, 4127, 4151, 4198, 4209, 4214, 4289, 4319], 'signals': [34, 61, 230, 257], 'governing': [35, 231], 'their': [36, 232, 473, 476, 860, 881, 896, 1166, 3819], 'assembly': [37, 64, 112, 233, 260, 308, 900, 1157, 1292], 'unknown.': [39, 235], 'Within': [40, 236], 'TRPV': [42, 238, 708, 852, 910, 1226], 'subgroup,': [43, 239], 'TRPV5': [44, 240, 912, 1113], 'and': [45, 51, 81, 116, 184, 241, 247, 277, 312, 380, 497, 500, 508, 535, 557, 563, 575, 604, 693, 705, 851, 913, 921, 973, 1105, 1112, 1117, 1155, 1168, 1272, 1316, 1384, 1389, 1428, 1447, 1452, 1462, 1657, 1746, 1788, 1861, 1878, 1958, 1973, 2011, 2103, 2134, 2139, 2148, 2156, 2167, 2177, 2181, 2219, 2227, 2290, 2300, 2370, 2385, 2398, 2405, 2434, 2455, 2470, 2474, 2483, 2502, 2529, 2556, 2607, 2627, 2636, 2780, 2881, 2914, 2994, 3028, 3055, 3138, 3162, 3237, 3325, 3338, 3378, 3493, 3532, 3593, 3608, 3633, 3649, 3679, 3687, 3697, 3767, 3794, 3821, 3841, 3867, 3893, 3954, 3978, 4005, 4032, 4039, 4086, 4154, 4163, 4212, 4251, 4302, 4329, 4369, 4392], 'TRPV6': [46, 114, 242, 310, 964, 1059, 1111, 1182, 1214, 1295, 1343, 1435, 1486, 1498, 1523, 2005, 2481, 2496, 2589, 2655, 2670, 2716, 2734, 2834, 2855, 2872, 2937, 3050, 3079, 3122, 3207, 3341, 3490, 3516, 3569, 3692, 3959, 3969, 4026, 4045, 4085, 4113, 4155, 4162, 4233, 4246], 'show': [47, 243, 915, 1275], 'exclusive': [48, 244], 'calcium': [49, 57, 245, 253, 919, 929, 1233, 4071, 4199], 'selectivity': [50, 246, 917], 'play': [52, 248], 'an': [53, 120, 186, 249, 316, 382, 968, 1194, 1260, 1368, 1424, 1548, 1922, 1942, 2016, 2676, 2893, 3211, 3238, 3790, 3936, 4148, 4378], 'important': [54, 250, 1300], 'role': [55, 251, 897], 'uptake.': [58, 254], 'To': [59, 255, 1339, 2807, 2848, 4058], 'identify': [60, 256, 2585], 'that': [62, 97, 143, 167, 177, 189, 258, 293, 339, 363, 373, 385, 586, 699, 961, 1110, 1183, 1193, 1276, 1394, 1543, 2021, 2032, 2591, 2631, 2672, 2828, 2857, 2873, 2940, 3022, 3123, 3168, 3277, 3439, 3621, 3746, 3771, 3883, 3926], 'mediate': [63, 259], 'of': [65, 113, 129, 134, 151, 154, 161, 261, 309, 325, 330, 347, 350, 357, 468, 484, 550, 560, 688, 815, 827, 886, 901, 906, 909, 963, 975, 1158, 1218, 1231, 1245, 1255, 1285, 1293, 1309, 1313, 1362, 1399, 1450, 1497, 1531, 1690, 1748, 1794, 1801, 1903, 1913, 1945, 2004, 2159, 2295, 2298, 2303, 2316, 2347, 2400, 2460, 2480, 2485, 2581, 2615, 2639, 2647, 2742, 2833, 2837, 2897, 2936, 3013, 3140, 3143, 3151, 3340, 3350, 3444, 3472, 3485, 3506, 3514, 3548, 3566, 3651, 3667, 3682, 3690, 3707, 3727, 3753, 3859, 3873, 3885, 3939, 3947, 3950, 3957, 3994, 4011, 4023, 4055, 4069, 4084, 4130, 4141, 4161, 4232, 4263, 4277, 4295, 4321, 4336, 4366], 'functional': [66, 193, 262, 389, 595, 1156, 1188, 1294], 'TRPV6,': [67, 263, 914], 'we': [68, 95, 264, 291, 1177, 1273, 1317, 2598, 2644, 2660, 2821, 2851, 2867, 3017, 3075, 3104, 3129, 3154, 3296, 3560, 3718, 3813], 'screened': [69, 265, 3105], 'domains': [70, 90, 266, 286, 566, 573, 1180, 3108, 3150, 3591, 3765, 3960], 'for': [71, 110, 192, 267, 306, 388, 602, 918, 970, 1153, 1164, 1291, 1325, 1453, 1478, 1509, 1529, 1722, 1739, 1759, 1767, 1782, 1891, 1927, 1949, 2038, 2097, 2105, 2145, 2342, 2390, 2477, 2570, 2594, 2814, 2885, 2943, 3010, 3106, 3126, 3332, 3663, 3799, 3915, 4036, 4304], 'self-association': [72, 268], 'using': [73, 269, 1333, 1374, 1470, 2241, 2450, 2509, 2533, 2602, 2907, 3109, 3283, 3301, 3973], 'co-immunoprecipitation,': [74, 270], 'sucrose': [75, 271, 2123], 'gradient': [76, 272, 2124], 'centrifugation,': [77, 273], 'bacterial': [78, 274, 2603, 3333, 3539], 'two-hybrid': [79, 275, 2244, 2281, 2604, 3334, 3540], 'assays,': [80, 276], 'patch': [82, 278], 'clamp': [83, 279, 1553, 1678], 'analysis.': [84, 280], 'Of': [85, 281, 683], 'two': [87, 283, 3474], 'identified': [88, 284], 'interaction': [89, 125, 285, 321, 871, 2605, 3612, 3659, 3739, 3749, 3778, 3796, 3916, 3952, 4042, 4051, 4149, 4207], 'within': [91, 137, 287, 333, 702, 706, 859, 1065, 1181, 2587, 3121, 3477, 3488, 3900], 'N-terminal': [93, 289, 1067, 1195, 2582, 3164, 3206, 3299, 3515, 3572, 3603, 3777], 'region,': [94, 290], 'showed': [96, 292, 3657, 3736, 3789], 'first': [99, 295, 3776], 'domain': [100, 296, 3120, 3604, 3613, 3757, 4052, 4123], 'encompassing': [101, 297, 3147], 'third': [103, 169, 299, 365, 1269, 3624], 'ankyrin': [104, 162, 300, 358, 856, 1063], 'repeat': [105, 131, 139, 171, 183, 301, 327, 335, 367, 379, 1288, 3214, 3240, 3271, 3355, 3383, 3448], 'is': [106, 190, 302, 386, 584, 875, 1162, 1199, 1298, 2033, 2673, 3124, 4203, 4271], 'stringent': [108, 304], 'requirement': [109, 305], 'physical': [111, 307, 1154], 'subunits': [115, 311, 588, 2856], 'when': [117, 313, 1119, 4094, 4184, 4195], 'transferred': [118, 314], 'unrelated': [121, 317, 1224], 'protein': [122, 318, 870, 1223, 1237, 2006, 2026, 2258, 2590, 2735, 3027, 3119, 3149, 3285, 3686, 3694, 3856, 3943, 4217], 'enables': [123, 319], 'its': [124, 320, 1066], 'TRPV6.': [127, 323, 1242, 1485, 3033, 3152, 3919, 4265], 'Deletion': [128, 324, 1413], 'this': [130, 138, 326, 334, 1175, 1207, 1219, 1236, 1246, 1286, 1479, 3922], 'mutation': [133, 329, 1371], 'critical': [135, 331, 2942], 'residues': [136, 332, 2935, 3505], 'rendered': [140, 336], 'nonfunctional': [141, 337], 'do': [144, 340, 2974], 'not': [145, 341, 873, 880, 1720, 2029, 2721, 2975, 3045, 3063, 3091, 3099, 3279, 3639, 3720, 3933, 4105, 4109, 4220], 'co-immunoprecipitate': [146, 342, 2853, 3046, 3064, 3078], 'tetramers.': [149, 345], 'Suppression': [150, 346], 'dominant-negative': [152, 348, 1211, 1253], 'inhibitors': [153, 349, 1827, 2136], 'TRPV6-specific': [155, 351], 'currents': [156, 352, 1668, 1694], 'was': [157, 353, 1248, 1351, 1372, 1407, 1481, 1489, 1512, 1544, 1886, 2045, 2232, 2259, 2444, 2982, 2988, 2997, 3098, 3278, 3535, 3638, 3797, 4073, 4133], 'achieved': [158, 354], 'by': [159, 355, 472, 1353, 1367, 1417, 1469, 1491, 1520, 1536, 1674, 1705, 1850, 1921, 1960, 2047, 2049, 2169, 2240, 2396, 2436, 2449, 2472, 2567, 4048, 4159], 'deletion': [160, 356, 1244, 1344], '(ANK)': [163, 359, 857], '3.': [164, 360], 'We': [165, 361, 1191, 2666, 3599], 'propose': [166, 362], 'ANK': [170, 182, 366, 378, 865, 1270, 1287, 1311, 3174, 3213, 3239, 3263, 3270, 3293, 3303, 3342, 3447, 3479, 3550, 3625, 3631, 3634, 3647, 3755, 3764, 3786, 3800, 3835, 3842, 3891, 3894, 4030, 4077, 4119, 4164, 4171, 4239, 4267, 4311, 4337, 4370], 'initiates': [172, 368], 'a': [173, 369, 466, 551, 568, 594, 825, 932, 1204, 1210, 1222, 1256, 1346, 1375, 1390, 1429, 1433, 1537, 1687, 2120, 2253, 2256, 2271, 2428, 2623, 2629, 2633, 2648, 2668, 2750, 2823, 2830, 2845, 2869, 2916, 3007, 3019, 3141, 3273, 3321, 3351, 3430, 3528, 3664, 3722, 3737, 3747, 3809, 3964, 4062, 4180, 4254, 4272], 'molecular': [174, 370, 2740, 2895, 4323, 4334], 'zippering': [175, 371], 'process': [176, 372], 'proceeds': [178, 374], 'past': [179, 375], 'fifth': [181, 377, 3269], 'creates': [185, 381], 'intracellular': [187, 383, 520, 555, 861, 3158, 3587], 'anchor': [188, 384], 'necessary': [191, 387, 1290], 'subunit': [194, 390, 553, 1230, 1258, 1326, 2596, 4037, 4068, 4153, 4202, 4211], 'assembly.': [195, 391], '1The': [396, 429], 'abbreviations': [397, 430], 'used': [398, 431, 1508, 2667, 2813, 2822, 2998, 3018, 3331, 3814], 'are:': [399, 432], 'TRP,': [400, 433], 'transient': [401, 434, 3136], 'potential;': [403, 436], 'ANK,': [404, 437], 'ankyrin;': [405, 438], 'aa,': [406, 439], 'amino': [407, 440, 1278, 2878, 2933, 3420, 3432, 3435, 3503, 3618, 3728], 'acid(s);': [408, 441], 'GFP,': [409, 442], 'green': [410, 443], 'fluorescent': [411, 444], 'protein;': [412, 445], 'EGFP,': [413, 446], 'enhanced': [414, 447], 'GFP;': [415, 448], 'HEK,': [416, 449], 'human': [417, 450], 'embryonic': [418, 451], 'kidney;': [419, 452], 'HA,': [420, 453], 'hemagglutinin;': [421, 454], 'F,': [422, 455], 'farad(s);': [423, 456], 'wt,': [424, 457], 'wild-type;': [425, 458], 'PM,': [426, 459], 'pore': [427, 460, 569], 'mutant.': [428, 461], 'have': [463, 2612], 'evolved': [464], 'as': [465, 494, 514, 967, 1301, 1569, 1714, 1976, 2007, 2009, 2137, 2844, 2910, 2926, 2999, 3072, 3100, 3112, 3281, 3305, 3975, 3980, 4157, 4179, 4342], 'family': [467, 487, 709], 'defined': [471], 'relatedness': [474], 'seminal': [477], 'member,': [478], 'Drosophila': [480], 'channel.': [482, 596], 'Members': [483], 'involved': [489, 926, 1185], 'sensory': [491], 'processes': [492, 512], 'such': [493, 513, 3282], 'invertebrate': [495], 'vision': [496], 'temperature,': [498], 'pain,': [499], 'gustatory': [501], 'sensation': [502], 'often': [503], 'restricted': [504], 'specialized': [506, 892], 'cells': [507, 1562, 1731, 1970, 2054, 2332, 2414, 2448, 2466, 2489, 2493, 2515, 2719, 2727, 2906, 2993, 3862, 3876, 3972, 4281, 4376], 'more': [510, 2634, 4144], 'ubiquitous': [511], 'sensing': [515], 'osmotic': [516], 'stress': [517], 'regulating': [519], 'magnesium': [521], 'concentration': [522], '(for': [523, 931], 'reviews,': [524], 'see': [525, 935, 2686, 3714, 3984], 'Refs.': [526, 2755], '1Montell': [527], 'C.': [528, 641, 739, 789, 831, 987, 1078, 1581, 2196, 2693, 2786, 2951, 2957], "Science's": [529, 832], 'STKE.': [530, 833], '2001;': [531, 623, 651, 834, 1001, 1034, 1092, 1595, 2965], '(http://stke.sciencemag.org/cgi/content/full/OC_sigtrans;2001/90/re1)PubMed': [532, 835], 'Google': [533, 545, 634, 657, 681, 736, 748, 770, 786, 810, 836, 847, 954, 1012, 1040, 1057, 1103, 1147, 1606, 1997, 2215, 2712, 2778, 2805, 2971, 3202, 3235, 3258, 3376, 3405, 3466], 'Scholar': [534, 2779], '2Clapham': [536, 838], 'D.E.': [537, 795, 839], 'Nature.': [538, 840], '2003;': [539, 675, 730, 780, 799, 841, 948, 1051, 1141, 2209, 2706, 2772, 2799], '426:': [540, 842], '517-524Crossref': [541, 843], 'PubMed': [542, 631, 654, 678, 733, 745, 767, 783, 807, 844, 951, 1009, 1037, 1054, 1100, 1144, 1603, 1996, 2212, 2709, 2775, 2802, 2968, 3199, 3232, 3255, 3373, 3402, 3463], 'Scopus': [543, 632, 655, 679, 734, 746, 768, 784, 808, 845, 952, 1010, 1038, 1055, 1101, 1145, 1604, 2213, 2710, 2776, 2803, 2969, 3200, 3233, 3256, 3374, 3403, 3464], '(2109)': [544, 846], 'Scholar).': [546, 682, 955, 1058, 1148, 1607, 2216, 2806, 3203, 3406, 3467], 'The': [547, 1264, 1370, 1403, 1439, 1460, 1476, 2929, 3309, 3627, 3881, 3999, 4170], 'general': [548], 'topology': [549], 'includes': [554], 'N-': [556], 'C-terminal': [558, 1515, 2931, 3159, 3322, 3529, 3580, 3810, 3965], 'regions': [559, 2586, 3165], 'variable': [561], 'length': [562, 3501], 'six': [564, 1061, 3170, 3262], 'transmembrane-spanning': [565], 'loop': [570, 3588], 'between': [571, 3589, 4075, 4150, 4208], 'transmembrane': [572, 3590], '5': [574, 1821, 2082, 2129, 3594, 3711], '6.': [576], 'In': [577, 3920], 'analogy': [578], 'voltage-gated': [580, 4070], 'potassium': [581], 'it': [583, 695, 957, 1106, 3043], 'four': [587, 1931], 'need': [589], 'assemble': [591], 'Tetramer': [597], 'formation': [598, 4320], 'has': [599, 696, 958, 1107], 'been': [600, 697, 959, 1108], 'shown': [601, 698, 960, 1109, 3827], 'TRPV1': [603], 'TRPV5/6': [605], '(3Kedei': [606], 'N.': [607], 'Szabo': [608], 'T.': [609, 662, 717, 983, 1043, 1074, 1128, 1577, 2759], 'Lile': [610], 'J.D.': [611], 'Treanor': [612], 'J.J.': [613], 'Olah': [614], 'Z.': [615], 'Iadarola': [616], 'M.J.': [617], 'Blumberg': [618], 'P.M.': [619, 3187], 'J.': [620, 645, 649, 668, 674, 723, 729, 756, 796, 998, 1089, 1134, 1140, 1592, 1990, 2194, 2691, 2765, 2771, 2784, 3251, 3398, 3459], 'Biol.': [621, 757, 797, 999, 1090, 1593], 'Chem.': [622, 758, 798, 1000, 1091, 1594], '276:': [624, 1002, 1093, 1596], '28613-28619Abstract': [625], 'Full': [626, 628, 762, 764, 802, 804, 1004, 1006, 1095, 1097, 1598, 1600], 'Text': [627, 629, 763, 765, 803, 805, 1005, 1007, 1096, 1098, 1599, 1601], 'PDF': [630, 766, 806, 1008, 1099, 1602], '(277)': [633], 'Scholar,': [635, 658, 737, 749, 771, 787, 837, 1013, 1041], '4Jahnel': [636], 'R.': [637], 'Dreger': [638], 'M.': [639, 751, 777, 1989, 2198, 2206, 2695, 2703, 2788, 2796, 3247, 3394, 3455], 'Gillen': [640], 'Bender': [642], 'O.': [643], 'Kurreck': [644], 'Hucho': [646], 'F.': [647, 666, 721, 1132, 2763], 'Eur.': [648], 'Biochem.': [650, 1030, 3192], '268:': [652], '5489-5496Crossref': [653], '(90)': [656], '5Hoenderop': [659, 2756], 'J.G.': [660, 715, 941, 1126, 2757], 'Voets': [661, 716, 1127, 2758], 'Hoefs': [663, 718, 1129, 2760], 'S.': [664, 719, 1130, 2761, 2963, 3183, 3227, 3368], 'Weidema': [665, 720, 1131, 2762], 'Prenen': [667, 722, 1133, 2764], 'Nilius': [669, 724, 942, 1135, 2766], 'B.': [670, 725, 943, 1136, 1983, 2767], 'Bindels': [671, 726, 944, 1137, 2768], 'R.J.': [672, 727, 945, 1138, 2769], 'EMBO': [673, 728, 1139, 2770, 3250, 3397, 3458], '22:': [676, 731, 1052, 1142, 2773], '776-785Crossref': [677, 732, 1143, 2774], '(293)': [680, 735, 1146, 2777], 'three': [685, 1342, 1854, 3173, 3825], 'major': [686], 'subfamilies': [687], 'TRPV,': [691], 'TRPC,': [692, 849], 'TRPM,': [694], 'some': [700], 'members': [701, 905], 'TRPC': [704], 'can': [710, 965, 1114, 2736, 2852, 4040, 4177, 4227], 'heteromeric': [712], '(5Hoenderop': [714, 1125], '6Montell': [738], 'Mol.': [740], 'Pharmacol.': [741], '1997;': [742], '52:': [743], '755-763Crossref': [744], '(120)': [747], '7Goel': [750], 'Sinkins': [752], 'W.G.': [753], 'Schilling': [754], 'W.P.': [755], '2002;': [759, 1993, 3229, 3252, 3370, 3399, 3460], '277:': [760], '48303-48310Abstract': [761], '(275)': [769], '8Amiri': [772], 'H.': [773, 995, 1049, 1086, 1589], 'Schultz': [774], 'G.': [775, 791], 'Schaefer': [776], 'Cell': [778, 946, 1991, 2207, 2403, 2406, 2704, 2797, 4275], 'Calcium.': [779, 947, 2208, 2705, 2798], '33:': [781, 949, 2210, 2707, 2800], '463-470Crossref': [782], '(64)': [785], '9Strubing': [788], 'Krapivinsky': [790, 792], 'L.': [793, 1017, 1981], 'Clapham': [794], '278:': [800, 3197], '39014-39019Abstract': [801], '(346)': [809], 'Scholar).So': [811], 'far': [812], 'almost': [813, 2889], 'all': [814, 3806, 3824], 'pores': [823], 'range': [826], 'different': [828, 1420, 1855, 2505, 2663, 3148, 3958], 'selectivities': [829], '(1Montell': [830], 'Scholar);': [848], 'TRPN,': [850], 'contain': [854, 3774, 3808], 'multiple': [855], 'repeats': [858, 866, 887, 1064, 1312, 3175, 3264, 3304, 3487], 'N': [862, 4128], 'termini.': [863], 'Although': [864], 'common': [868], 'modular': [869], 'motifs,': [872], 'much': [874], 'regarding': [877], 'whether': [878, 2850], 'specific': [882, 1277], 'sequence': [883, 1496, 1528, 3208, 3349, 3491, 3654], 'and/or': [884], 'number': [885, 1337, 2399, 3494], 'required': [889, 2593], 'interactions': [893, 2601, 2620, 3565, 3928], 'about': [895], 'channels.': [903, 1190, 1296], 'Two': [904], 'subgroup': [908], 'exquisite': [916], 'ions': [920], 'likely': [923], 'be': [925, 1320, 2737, 2902, 4046, 4110, 4228], 'epithelial': [928], 'uptake': [930], 'recent': [933], 'review,': [934], 'Ref.': [936, 1979, 2687], '10den': [937], 'Dekker': [938], 'E.': [939, 993, 1084, 1587], 'Hoenderop': [940], '497-507Crossref': [950], '(179)': [953], 'Moreover': [956, 1216], 'expression': [962, 1510, 1736, 3139, 3517, 3706, 3820, 4380], 'act': [966, 4178], 'indicator': [969], 'malignancy': [972], 'invasiveness': [974], 'prostate': [976], 'cancer': [977], '(11Wissenbach': [978, 1069, 1572], 'U.': [979, 1045, 1070, 1573, 2202, 2699, 2792, 2953, 2962, 3226, 3367], 'Niemeyer': [980, 1071, 1574], 'B.A.': [981, 1072, 1575, 2949], 'Fixemer': [982, 1073, 1576], 'Schneidewind': [984, 1075, 1578], 'A.': [985, 989, 1076, 1080, 1579, 1583, 2964, 3228, 3369], 'Trost': [986, 1077, 1580, 2956], 'Cavalie': [988, 1079, 1582], 'Reus': [990, 1081, 1584], 'K.': [991, 1082, 1585], 'Meese': [992, 1083, 1586], 'Bonkhoff': [994, 1048, 1085, 1588], 'Flockerzi': [996, 1046, 1087, 1590, 2203, 2700, 2793, 2954], 'V.': [997, 1047, 1088, 1591, 2204, 2701, 2794, 2955], '19461-19468Abstract': [1003, 1094, 1597], '(234)': [1011, 1102, 1605], '12Peng': [1014], 'J.B.': [1015, 3177], 'Zhuang': [1016], 'Berger': [1018, 3180], 'U.V.': [1019, 3181], 'Adam': [1020], 'R.M.': [1021], 'Williams': [1022], 'B.J.': [1023], 'Brown': [1024, 3188], 'E.M.': [1025, 3189], 'Hediger': [1026, 3190], 'M.A.': [1027, 3191], 'Freeman': [1028], 'M.R.': [1029], 'Biophys.': [1031, 3193], 'Res.': [1032, 3194], 'Commun.': [1033, 3195], '282:': [1035], '729-734Crossref': [1036], '(152)': [1039], '13Fixemer': [1042], 'Wissenbach': [1044, 2201, 2698, 2791, 2952], 'Oncogene.': [1050], '7858-7861Crossref': [1053], '(201)': [1056], 'contains': [1060, 1267, 3169, 3527, 3622], 'putative': [1062, 3549], 'region': [1068, 1196, 1208, 1220, 1247, 1266, 3573, 3581, 3616, 4027, 4175], 'Scholar),': [1104, 3377], 'homotetramers': [1116], 'heterotetramers': [1118], 'overexpressed': [1120, 3940], 'alone': [1121, 3788, 3802, 3844, 4034, 4218, 4368], 'together,': [1123], 'respectively': [1124], 'Analyzing': [1149], 'structure': [1151, 3337, 3449], 'responsible': [1152, 3125], 'these': [1159, 3261, 4060], 'essential': [1163, 2419], 'understanding': [1165], 'function': [1167, 1308], 'may': [1169, 1318, 3924], 'facilitate': [1170], 'designing': [1171], 'drug': [1173], 'targets.In': [1174], 'study': [1176], 'analyzed': [1178, 1959, 2168], 'assembling': [1187], 'found': [1192], '(aa': [1197, 2938, 3160, 3166, 3574, 3582, 3595, 3636, 3655, 3769, 3839, 3845, 4081, 4124, 4193], '116–191)': [1198, 3770, 3840, 4082, 4125, 4194], 'sufficient': [1200, 4035, 4134, 4204], 'enable': [1202, 4147, 4206], 'self-interaction;': [1203], 'construct': [1205, 1488, 2891, 2987, 3051, 3526, 3669, 3708, 3743, 3762, 4053], 'containing': [1206, 1379, 1423, 2270, 2422, 3763], 'had': [1209], 'effect': [1212], 'on': [1213, 1305, 1555, 1743, 1791, 2164, 2288, 2352, 2499, 2526, 3156], 'currents.': [1215], 'transfer': [1217], 'proteins,': [1227], 'γ1': [1229, 4067, 4131, 4201, 4210], 'voltage-activated': [1232], 'enabled': [1235], 'physically': [1239], 'interact': [1240, 4221], 'Conversely': [1243, 3057], 'able': [1249, 1321, 2617, 3562], 'suppress': [1251], 'phenotype': [1254], 'mutant': [1257], 'altered': [1261], 'ionic': [1262], 'pore.': [1263], 'interacting': [1265, 3087, 3107], 'repeat,': [1271], 'could': [1274, 2901, 3039, 3076, 3719, 4108], 'acids': [1279, 2879, 3421, 3436, 3619], 'second': [1282, 3748], 'helical': [1283, 3475, 3509], 'segment': [1284], 'This': [1297, 2888], 'particularly': [1299], 'no': [1302, 3795, 4224], 'data': [1303], 'exist': [1304], 'physiological': [1307], 'now': [1319], 'establish': [1323], 'rules': [1324], 'assembly.EXPERIMENTAL': [1327], 'PROCEDURESSite-directed': [1328], 'Mutagenesis—All': [1329], 'experiments': [1330, 1455], 'were': [1331, 1415, 1442, 1456, 1467, 1518, 1563, 1669, 1703, 1719, 1732, 1848, 1870, 1898, 1939, 1971, 2060, 2117, 2153, 2162, 2173, 2186, 2222, 2238, 2276, 2286, 2333, 2350, 2375, 2394, 2415, 2467, 2497, 2516, 2564, 3318, 3327, 3561, 3831, 3847, 4249, 4282], 'conducted': [1332], 'hTRPV6': [1334], '(GenBank™': [1335], 'accession': [1336], 'NM_018646).': [1338], 'obtain': [1340], 'mutants,': [1345], 'MluI': [1347, 1425, 1446], 'restriction': [1348, 1437], 'site': [1349, 1383, 1398, 1427, 2840, 3725, 3750, 3779], '(ACGCGT)': [1350], 'introduced': [1352, 1373], 'PCR': [1354, 1377, 1391, 1418], 'at': [1355, 1364, 1695, 1770, 1785, 1888, 1894, 1916, 1952, 2100, 2108, 2335, 2387, 2679, 2739, 4307], 'nucleotide': [1356], 'positions': [1357, 3438], '333–335': [1358], 'resulting': [1359], 'replacement': [1361], 'serine': [1363], 'position': [1365, 3471, 3484, 3547], '112': [1366], 'arginine.': [1369], 'reverse': [1376, 1430], 'primer': [1378, 1393, 1431], 'downstream': [1381, 3726, 3752], 'SacI': [1382, 1397], 'indicated': [1386], 'base': [1387], 'pairs': [1388, 3495], 'forward': [1392, 1421], 'included': [1395, 2000, 3670], 'pCAGGS-IRES-EGFP': [1401, 1503, 2037], 'vector.': [1402, 1412, 1504], 'SacI-cut': [1404, 1410], 'amplified': [1405, 1506], 'fragment': [1406, 3629, 3643, 4083], 'ligated': [1408, 1443], 'into': [1409, 1444, 1458, 1502, 1540, 2262, 2446, 2658, 3329, 3605], 'pCAGGS-TRPV6': [1411], 'constructs': [1414, 1511, 1517, 2275, 2508, 3089, 3300, 3317, 3807, 3826, 3996], 'made': [1416, 2565, 2645], 'primers': [1422], 'recognition': [1426], 'covering': [1432, 3630, 3644, 3890], 'unique': [1434], 'EcoRV': [1436, 1448], 'site.': [1438], 'MluI/EcoRV': [1440], 'fragments': [1441, 1524, 3607, 3944], 'sites': [1449], 'TRPV6-pCAGGS-IRES-EGFP': [1451], 'precipitation': [1454], 'recloned': [1457], 'TRPV6-EGFP-pCDNA3.': [1459], 'D542A': [1461], '138AR139': [1463], '138EA139': [1465], 'mutations': [1466], 'obtained': [1468, 1490, 1519, 2174], 'QuikChange': [1472], 'XL': [1473], 'kit': [1474], '(Stratagene).': [1475], 'template': [1477], 'reaction': [1480], 'EGFP-tagged': [1483, 1516, 2486, 2506, 2664, 2871, 2986, 3031, 3048, 3088, 3144, 3628, 3995], 'full-length': [1484, 2890, 3032, 3049, 3568, 3918, 4044], 'antisense': [1487, 1500], 'cloning': [1492], 'entire': [1494], 'coding': [1495, 1551], 'orientation': [1501], 'All': [1505, 2171, 2274], 'DNA': [1507, 2251], 'sequenced.Recombinant': [1513], 'Proteins—All': [1514], 'inserting': [1521], 'PCR-amplified': [1522], 'including': [1525, 4029], 'consensus': [1527, 3241, 3339, 3348, 3384], 'initiation': [1530], 'translation': [1532], 'vertebrates': [1534], 'followed': [1535, 1920], 'start': [1538], 'ATG': [1539], 'pCDNA3': [1541, 2442], 'vector': [1542, 2268, 2443, 4381], 'modified': [1545, 2266], 'include': [1547], 'in-frame': [1549, 2261], 'EGFP': [1550, 2031, 3323, 3530, 3811, 3857, 3967, 4003, 4088, 4225, 4316, 4367, 4379], 'sequence.Electrophysiology—Patch': [1552], 'recordings': [1554], 'single': [1556], 'transfected': [1557, 1733, 1968, 2055, 2445, 2503, 2662, 2868, 3085, 3861, 4278, 4375], 'HEK': [1558, 1728, 1969, 2491, 2649, 2725, 2991, 4279], '293': [1559, 1729, 2053, 2409, 2488, 2492, 2650, 2726, 2992, 4280], 'HEK-TRPV6': [1561], 'performed': [1564, 2239], '48–72': [1565], 'h': [1566, 1741, 2065, 2099, 2147, 2512], 'after': [1567, 1763, 2066, 2513, 3135, 3385, 3930, 4230], 'transfection': [1568, 2452, 3137], 'described': [1570, 1977, 3133], 'previously': [1571], 'Pipette': [1608], 'solution': [1609, 1632], 'contained': [1610, 1633], '140': [1611], 'mm': [1612, 1616, 1619, 1622, 1625, 1635, 1639, 1642, 1645, 1648, 1651, 1659, 1806, 1809, 1822, 1832, 1836, 1845, 2072, 2075, 2083, 2089, 2093, 2127, 2130, 2321, 2325, 2329, 2368, 4297], 'aspartic': [1613], 'acid,': [1614, 1874], '10': [1615, 1618, 1624, 1638, 1647, 1672, 2320, 2324, 4296, 4399], 'EGTA,': [1617], 'NaCl,': [1620, 1807, 2073], '1': [1621, 1760, 1835, 1844, 2085, 2092, 2098, 2146, 2314, 3632], 'MgCl2,': [1623, 1646], 'Hepes': [1626, 1652], '(pH': [1627, 1653], '7.2': [1628], 'CsOH).': [1630], 'Bath': [1631], '115': [1634], 'triethylammonium': [1636], 'chloride,': [1637], 'CsCl,': [1640], '2.8': [1641], 'KCl,': [1643], '2': [1644, 3635], 'glucose,': [1649], '20': [1650, 2126, 2328, 2367], '7.4': [1654], 'NaOH),': [1656], '30': [1658, 2106, 2149, 2336, 2388, 2571], 'CaCl2.': [1660], 'After': [1661, 1865, 1930, 2312], 'obtaining': [1662], 'whole': [1663, 1666, 1707], 'cell': [1664, 1667, 1708, 1796, 2625, 2651, 2865, 3020, 3083], 'configuration,': [1665], 'recorded': [1670], 'every': [1671], 's': [1673], 'applying': [1675], '100-ms': [1676], 'voltage': [1677], 'ramps': [1679], 'from': [1680, 1686, 2035, 2175, 2223, 2233, 2717, 2722, 2904, 3080, 3970], '–100': [1681], 'mV': [1682, 1685, 1692, 1697], '+100': [1684], 'holding': [1688], '+70': [1691], 'until': [1693], '–80': [1696], 'reached': [1698], 'maximum': [1699], 'values.': [1700], 'Currents': [1701], '(pA)': [1702], 'divided': [1704], 'capacitance': [1709], '(pF).': [1710], 'Data': [1711], 'given': [1713], 'mean': [1715], '±': [1716], 'S.E.': [1717], 'Values': [1718], 'corrected': [1721], 'liquid': [1723], 'junction': [1724], 'potentials.Co-immunoprecipitation': [1725], 'Experiments—TRPV6': [1726], 'stable': [1727, 2864, 3082], '(TRPV6s)': [1730], 'pCDNA3-TRPV6-EGFP': [1735], 'constructs,': [1737], 'cultured': [1738], '24–72': [1740], 'depending': [1742, 1790], 'condition': [1745], 'fluorescence': [1747, 2534, 3815, 3858, 4010], 'cells,': [1750, 4098], 'washed': [1751, 1775, 1875, 1972, 2517], 'once': [1752, 1776, 2518], 'phosphate-buffered': [1754, 1779, 2520, 2524], 'saline,': [1755, 1780, 2521], 'treated': [1756, 1948, 1974, 2061], 'trypsin': [1758, 2063], 'min,': [1761], 'pooled': [1762], 'adding': [1764, 2313, 4238], 'medium,': [1765, 2331], 'centrifuged': [1766, 1781, 1887, 2104], '7': [1768, 1783], 'min': [1769, 1784, 1893, 1951, 2107, 2572, 4306], '1000': [1771, 1786], 'rpm': [1772, 1890, 2110, 2143], '(4': [1773], '°C),': [1774], 'ice-cold': [1778, 1802], 'rpm,': [1787], 'resuspended': [1789, 1879, 1940], 'size': [1793], 'pellets': [1797], '1–2': [1799, 1928], 'ml': [1800, 2315], 'RIPA': [1803, 1936], 'buffer': [1804, 2070, 4287], '(150': [1805, 2071], '50': [1808, 2074, 2296, 2301, 2364], 'Tris-HCl,': [1810, 2076], 'pH': [1811, 2077], '8.0,': [1812], '0.5%': [1813, 2079], 'sodium': [1814, 2080], 'deoxycholate,': [1815, 2081], '1%': [1816], 'Nonidet': [1817], 'P40,': [1818], '0.1%': [1819, 2132], 'SDS,': [1820, 4389, 4398], 'EDTA)': [1823], 'added': [1825, 2376], 'protease': [1826, 2135], '(1': [1828], 'μg/ml': [1829, 1839, 2086, 2356, 2359, 2362, 2365, 2462], 'leupeptin,': [1830, 2087], '0.1': [1831, 2088, 2574], 'phenylmethylsulfonyl': [1833, 2090], 'fluoride,': [1834, 2091], 'pepstatin': [1837, 2094], 'Aor1': [1838], 'antipain,': [1840], '0.3': [1841], 'μm': [1842, 2575], 'aprotinin,': [1843], 'benzamidine).': [1846], 'Cells': [1847], 'lysed': [1849], 'sequential': [1851], 'pipetting': [1852], 'through': [1853], 'gauge': [1856], 'syringe': [1857], 'needles': [1858], '(0.7,': [1859], '0.55,': [1860], '0.4': [1862], 'inner': [1863], 'diameters).': [1864], 'lysis,': [1866], '50–100': [1867], 'μlofthe': [1868], 'lysate': [1869, 1885], 'precipitated': [1871, 2046, 3691], 'trichloroacetic': [1873, 3673], 'acetone,': [1877], 'loading': [1881, 1946, 4286], 'buffer;': [1882], 'remaining': [1884], '14,000': [1889], '15': [1892, 2358], '4': [1895, 1917, 2044, 2101, 2371, 3592, 3768, 3801, 3838, 3897, 4033, 4080, 4122, 4174, 4192, 4242, 4270, 4314], '°C.': [1896, 4309], 'Supernatants': [1897], 'incubated': [1899, 2096, 2334, 2386], '2.2': [1901], 'μg': [1902, 1912], 'affinity-purified': [1904], 'polyclonal': [1905, 2669, 3059], 'anti-TRPV6': [1906, 2183, 2924, 4256], 'antibody': [1907, 1915, 2018, 2231, 2671, 2826, 2886, 2908, 2913, 2919, 2995, 3002, 3038, 3060, 3071, 3097, 3110, 3701, 3977, 4235, 4257], '(antibody': [1908, 2179, 2684], '429)': [1909, 2180], '3': [1911, 1950, 2042, 3648, 3756, 3766, 3787, 3836, 3843, 3892, 3895, 4031, 4078, 4120, 4165, 4172, 4190, 4240, 4268, 4305, 4312, 4338, 4371], 'anti-HA': [1914, 2220, 3037], '°C': [1918, 2337, 2389], 'overnight': [1919], 'incubation': [1923, 2569], 'protein-A-Sepharose': [1925], 'beads': [1926, 1938], 'h.': [1929, 2344, 2392], 'five': [1933, 3292], 'washes': [1934], 'buffer,': [1937, 1947], 'equal': [1943, 4261], 'volume': [1944], '95': [1953, 4308], '°C,': [1954, 2102, 2430], 'subjected': [1955, 2140], 'SDS-PAGE,': [1957], 'Western': [1961, 2475, 2819, 4356], 'blot': [1962, 2014, 2476], 'analysis.Cell': [1963], 'Surface': [1964], 'Biotinylation—Dishes': [1965], 'confluent': [1967], 'exactly': [1975], '14Mery': [1980], 'Strauss': [1982], 'Dufour': [1984], 'J.F.': [1985], 'Krause': [1986], 'K.H.': [1987], 'Hoth': [1988], 'Sci.': [1992, 2961, 3225, 3366], '115:': [1994], '3497-3508Crossref': [1995], 'Scholar.': [1998], 'Controls': [1999, 3662], 'monitoring': [2001, 3671], 'input': [2003, 3675], 'well': [2008], 'stripping': [2010, 3696], 'reprobing': [2012, 3698], 'anti-GFP': [2017, 2218, 2918, 3070, 3979], 'control': [2020, 3009], 'only': [2022, 2859, 3781, 3906], 'TRPV6-EGFP': [2024, 2057], 'fusion': [2025, 3145, 3685, 3855, 3888, 4216], '(+5p2)': [2027], 'co-expressed': [2034], 'TRPV6-wt': [2039], 'TRPV6-ΔANK': [2041], '+': [2043, 2319, 2323, 2327, 3426, 3712, 3837, 3896, 4079, 4121, 4166, 4173, 4191, 4241, 4269, 4313, 4339, 4372], 'avidin-agarose.Sedimentation': [2048], 'Sucrose': [2050], 'Gradient': [2051], 'Centrifugation—HEK': [2052], 'TRPV6-M5-EGFP': [2059], '48': [2064], 'transfection,': [2067, 2514], 'solubilized': [2068, 4284], '8,': [2078], 'EDTA,': [2084, 2131], 'A),': [2095], '52,000': [2109], '(TLA100)': [2111], 'pellet': [2113], 'undissolved': [2114], 'material.': [2115], 'Samples': [2116], 'loaded': [2118], 'onto': [2119], 'continuous': [2121], '10–20%': [2122], '(in': [2125], 'Tris,': [2128], 'Triton-X-100,': [2133], 'above)': [2138], '50,000': [2142], '(NVT65)': [2144], 'min.': [2150], '660-μl': [2151], 'fractions': [2152], 'then': [2154], 'collected,': [2155], '35': [2157], 'μl': [2158, 2346], 'each': [2160, 2348, 2378, 3961], 'fraction': [2161], 'resolved': [2163], 'SDS-polyacrylamide': [2165, 2752], 'gels': [2166], 'immunoblotting.': [2170], 'markers': [2172], 'Sigma.Antibodies': [2176], 'Reagents—Polyclonal': [2178], 'monoclonal': [2182, 2825, 2917, 2923, 3069, 3096, 4255], '(26B3)': [2184, 2827, 2925, 4258], 'antibodies': [2185, 2221], 'generated': [2187], 'our': [2189], 'laboratory': [2190], '(15Hirnet': [2191], 'D.': [2192, 2689, 2782], 'Olausson': [2193, 2690, 2783], 'Fecher-Trost': [2195, 2692, 2785], 'Bodding': [2197, 2694, 2787], 'Nastainczyk': [2199, 2696, 2789], 'W.': [2200, 2697, 2790], 'Freichel': [2205, 2702, 2795], '509-518Crossref': [2211, 2708, 2801], '(65)': [2214, 2711, 2804], 'Monoclonal': [2217], 'Roche': [2224], 'Applied': [2225], 'Science,': [2226], 'horseradish': [2228], 'peroxidase-coupled': [2229], 'anti-mouse': [2230], 'Jackson': [2234], 'Laboratories.Bacterial': [2235], 'Two-hybrid': [2236], 'Assay—Experiments': [2237], 'Bacteriomatch': [2243], 'system': [2245, 2282, 2630, 3541], '(Stratagene)': [2246], 'following': [2249], 'modifications.': [2250], 'bait': [2254], 'target': [2257], 'cloned': [2260], 'pTRG': [2263, 2299], 'pBT': [2267], '(pBTL)': [2269], '(Gly4-Ser)3': [2272], 'linker.': [2273], 'sequenced.': [2277], 'Electrocompetent': [2278], 'bacteria': [2279], '(Bacteriomatch': [2280], 'reporter': [2283], 'strain,': [2284], 'Stratagene)': [2285], 'thawed': [2287], 'ice': [2289], 'electroporated': [2291], 'presence': [2294, 2459, 2479, 4290], 'ng': [2297, 2302], 'pBTL': [2304], '(Bio-Rad;': [2305], '2.5': [2306], 'kV,': [2307], '200': [2308], 'ohms,': [2309], '25': [2310], 'μF).': [2311], 'SOC': [2317], '(LB': [2318], 'MgCl2': [2322], 'MgSO4': [2326], 'glucose)': [2330], 'shaking': [2339], '(225': [2340], 'rpm)': [2341], '1.5': [2343], '100': [2345], 'transformation': [2349], 'plated': [2351], 'CTCK-agar': [2353], 'plates': [2354], '(600': [2355], 'carbenicillin,': [2357], 'tetracycline,': [2360], '34': [2361], 'chloramphenicol,': [2363], 'kanamycin,': [2366], 'isopropyl-1-thio-β-d-galactopyranoside,': [2369], 'mg/ml': [2372], '5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside': [2373], '(X-gal)': [2374], 'plate': [2379], 'shortly': [2380], 'before': [2381], 'plating': [2382], 'bacteria)': [2384], '24': [2391], 'Interactions': [2393], 'detected': [2395, 2738, 2983, 3291, 4229], 'growth': [2397], 'blue': [2401], 'colonies.Stable': [2402], 'Lines': [2404], 'Culture': [2407], 'Conditions—HEK': [2408], '(CRL-1573,': [2410], 'ATCC,': [2411], 'Manassas,': [2412], 'VA)': [2413], 'maintained': [2416], 'minimal': [2418], 'medium': [2420], '(Invitrogen)': [2421], '10%': [2423], 'fetal': [2424], 'calf': [2425], 'serum': [2426], '37': [2429, 4390], '5%': [2431], 'CO2': [2432], 'incubator': [2433], 'passaged': [2435], 'treatment': [2437, 4303], 'trypsin.': [2439], 'An': [2440], 'hTRPV6-containing': [2441], 'Super-Fect': [2451], 'reagent': [2453], '(Qiagen)': [2454], 'selected': [2456], '500': [2461], 'Geneticin': [2463], '(Invitrogen).': [2464], 'Surviving': [2465], 'isolated,': [2468], 'subcloned,': [2469], 'tested': [2471], 'Northern': [2473], 'transcript': [2482], 'protein.Immunofluorescence': [2484], 'Constructs—HEK': [2487], 'stably': [2494, 2653, 3023], 'expressing': [2495, 2654], 'grown': [2498], 'poly-d-lysine-coated': [2500], 'coverslips': [2501], 'cDNA': [2507], 'SuperFect.': [2510], '24–48': [2511], 'mounted': [2522], 'saline': [2525], 'glass': [2527], 'slides,': [2528], 'imaged': [2530], 'vivo': [2532, 3992], 'microscopy': [2535, 3816], '(Nikon': [2536], 'E600,': [2537], 'PlanApo': [2538], '60×': [2539], 'A/1.40': [2540], 'oil': [2541], 'attached': [2543, 2551], 'DN100': [2544], 'Olympus': [2546], 'BX-50,': [2547], 'UPlanApo': [2548], '100×/1.35': [2549], 'imago': [2552], 'camera': [2553], '(T.I.L.L.': [2554], 'Photonics))': [2555], 'analySIS': [2557], 'aquisition': [2558], 'software': [2559], '(Soft': [2560], 'Imaging': [2561], 'System).': [2562], 'Mitochondria': [2563], 'visible': [2566, 4331], 'prior': [2568], 'Texas': [2576, 3868, 4015], 'Red': [2577, 3869, 4016], 'Mito-Tracker': [2578], '(Molecular': [2579], 'Probes).RESULTSIdentification': [2580], 'Interaction': [2583], 'Domains—To': [2584], 'homomultimeric': [2595], 'assembly,': [2597], 'investigated': [2599, 3536, 4364], 'protein-protein': [2600], 'screens': [2606], 'co-immunoprecipitation': [2608, 2642, 3015, 3558, 3956, 4160], 'experiments.': [2609], 'approaches': [2611], 'advantage': [2614], 'being': [2616, 3272], 'investigate': [2619], 'both': [2621, 3210, 4136, 4382], 'mammalian': [2624], 'environment': [2626], 'allows': [2632], 'rapid': [2635], 'independent': [2637], 'dissection': [2638], 'binding': [2640, 2944, 3724], 'sites.For': [2641], 'experiments,': [2643, 3016, 3559], 'use': [2646], 'line': [2652, 2866, 3021, 3084], '(TRPV6s': [2656], 'cells)': [2657], 'which': [2659, 3290, 3316, 3326, 3525, 3533], 'transiently': [2661, 2860, 3029], 'constructs.': [2665], 'directed': [2674], 'against': [2675], 'epitope': [2677, 2832, 2884], 'localized': [2678, 4099, 4185], 'very': [2681, 2930, 3782], 'C': [2682, 3054], 'terminus': [2683, 4129], '429,': [2685], '15Hirnet': [2688, 2781], 'Scholar)': [2713, 2972, 3236, 3259], 'precipitate': [2715, 3040], 'TRPV6s': [2718, 3971, 4097], 'parental': [2724], '(Fig.': [2728, 2920, 3003, 3052, 3066, 3265, 3307, 3597, 3660, 3676, 3758, 3784, 3803, 4090, 4114, 4168], '1A).': [2729], 'Due': [2730, 3703], 'post-translational': [2732], 'glycosylation,': [2733], 'masses': [2741, 4335], 'around': [2743, 3617], '75': [2744], 'up': [2745], '85–100': [2747], 'kDa': [2748, 2900], 'denaturing': [2751, 4385, 4394], 'gel': [2753], '(see': [2754, 2841], 'avoid': [2808], 'detecting': [2809, 3981], 'rabbit': [2811], 'immunoglobulins': [2812], 'immunoprecipitation': [2816, 3931, 4231], 'blot,': [2820], 'mouse': [2824], 'detects': [2829], 'distinct': [2831], 'further': [2835, 3723], 'upstream': [2836], '429': [2839, 2909, 2996, 3061, 3111, 3974], '“Experimental': [2842], 'Procedures”)': [2843], 'detection': [2846, 2927], 'antibody.': [2847, 2928], 'determine': [2849], 'individual': [2854], 'expressed': [2861, 2989, 3668, 3684, 4095], 'C-terminally': [2870], 'lacks': [2874], 'last': [2876], '32': [2877, 2932], '(TRPV6-Δ-EGFP)': [2880], 'thus': [2882, 2973, 4176], '429.': [2887, 4236], 'estimated': [2894], 'mass': [2896, 4324], '∼': [2898], '110': [2899], 'co-immunoprecipitated': [2903, 3780, 4111], 'TRPV6-expressing': [2905, 4187], 'precipitating': [2912, 3001, 3073, 3114, 3976], 'either': [2915, 4283], '1B)': [2921], 'acid': [2934, 3354, 3504, 3729], '694–725)': [2939], 'regulatory': [2946], 'calmodulin': [2947], '(16Niemeyer': [2948], 'Bergs': [2950], 'Proc.': [2958, 3222, 3363], 'Natl.': [2959, 3223, 3364], 'Acad.': [2960, 3224, 3365], '98:': [2966], '3600-3605Crossref': [2967], '(148)': [2970], 'contribute': [2976], 'TRPV6-TRPV6': [2978, 3127], 'interaction.': [2979], 'No': [2980], 'signal': [2981, 4183, 4226], 'if': [2984], '1B,': [3004], 'right': [3005, 3498, 3523, 3879, 4007], 'lane).As': [3006], 'separate': [3008], 'specificity': [3012], 'expresses': [3024, 3030], 'HA-tagged': [3025], 'TRPC3': [3026], 'As': [3034, 3805], 'expected,': [3035], 'TRPC3,': [3041], 'did': [3044, 3062], '1,': [3053], 'D).': [3056], 'hTRPC3': [3065], '1D).Using': [3067], 'antibody,': [3074, 3982], 'also': [3077, 3735, 4363], '(data': [3090, 4104], 'shown).': [3092], 'However,': [3093, 4116], 'because': [3094], 'efficient': [3101], 'immunoprecipitating,': [3103], 'antibody.To': [3115], 'narrow': [3116], 'down': [3117, 3610], 'interaction,': [3128, 3793, 4038], 'repeated': [3130], 'experiment': [3132], 'above': [3134], 'series': [3142], 'First': [3153], 'focused': [3155], '584–694)': [3161, 3583], '1–328)': [3167, 3575], 'rather': [3171], 'than': [3172, 3742], '(17Peng': [3176], 'Chen': [3178], 'X.Z.': [3179], 'Weremowicz': [3182], 'Morton': [3184], 'C.C.': [3185], 'Vassilev': [3186], '2000;': [3196], '326-332Crossref': [3198], '(183)': [3201], 'Aligning': [3204], 'idealized': [3212], '(18Mosavi': [3215, 3356], 'L.K.': [3216, 3357], 'Minor': [3217, 3358], 'Jr.,': [3218, 3359], 'D.L.': [3219, 3360], 'Peng': [3220, 3361], 'Z.Y.': [3221, 3362], '99:': [3230, 3371], '16029-16034Crossref': [3231, 3372], '(282)': [3234, 3375], '(19Michaely': [3242, 3389, 3450], 'P.': [3243, 3390, 3451], 'Tomchick': [3244, 3391, 3452], 'D.R.': [3245, 3392, 3453], 'Machius': [3246, 3393, 3454], 'Anderson': [3248, 3395, 3456], 'R.G.': [3249, 3396, 3457], '21:': [3253, 3400, 3461], '6387-6396Crossref': [3254, 3401, 3462], '(169)': [3257, 3404, 3465], 'revealed': [3260], '2A)': [3266], 'somewhat': [3274], 'atypical': [3275], 'one': [3276, 3534, 3611], 'recognized': [3280], 'prediction': [3286], 'program': [3287], 'SMART': [3288], '(smart.embl-heidelberg.de/embl),': [3289], 'repeats.': [3294, 3343], 'Subsequently': [3295], 'subdivided': [3297, 3601], 'guideposts': [3306], '2B).': [3308], 'check': [3310, 3519], 'marks': [3311, 3520], 'Fig.': [3313, 3715, 3829, 3851, 3985], '2B': [3314], 'indicate': [3315, 3410, 3416, 3422, 3469, 3483, 3499, 3524, 3545, 3925], 'subcloned': [3319, 3328], 'tag': [3324, 3531, 4089], 'vectors': [3330], 'analysis.Fig.': [3335], '2A,': [3336], 'Ank': [3344, 3379], 'c1': [3345], 'shows': [3346, 3381, 3853, 4002, 4009], 'designed': [3352], '33-amino': [3353], 'c2': [3380, 3428], 'Michaely': [3386], 'et': [3387], 'al.': [3388], 'Dark': [3407], 'gray': [3408, 3414], 'boxes': [3409, 3415, 3544], 'identical': [3411], 'residues,': [3412, 3418], 'lighter': [3413], 'conserved': [3417], 'framed': [3419], 'non-conserved': [3423], 'sites,': [3424], 'indicates': [3429], 'non-polar': [3431], 'acid.': [3433], 'Underlined': [3434], 'face': [3440], 'convex': [3442], 'surface': [3443], 'three-dimensional': [3446], 'Cylinders': [3468], 'segments': [3476], 'repeat.': [3480, 3626], 'Subscript': [3481], 'numbers': [3482], '(V6)': [3492], 'predicted': [3508], 'segments.': [3510], 'B,': [3511, 3955, 4237], 'schematic': [3512], 'representation': [3513], 'constructs;': [3518], '(B2H).': [3542], 'Numbered': [3543], 'repeats.View': [3551], 'Large': [3552, 4018, 4358], 'Image': [3553, 4019, 4359], 'Figure': [3554, 4020, 4360], 'ViewerDownload': [3555, 4021, 4361], '(PPT)In': [3556], 'detect': [3564], 'neither': [3577], 'nor': [3584], '466–493)': [3596], '3A).': [3598, 3661, 3759], 'therefore': [3600], 'shorter': [3606], 'narrowed': [3609], '116–163': [3620], '1–115)': [3637], 'immunoprecipitated,': [3640], 'addition': [3646], 'part': [3650], 'adjacent': [3653], '1–163)': [3656], 'positive': [3658, 3738], 'comparable': [3665], 'amount': [3666, 3689], 'acid-precipitated': [3674], '3A,': [3677, 3716], 'bottom)': [3678], 'immunoprecipitant-supernatant': [3681], 'wild-type': [3693], '(by': [3695], 'blots': [3699], '26B3).': [3702], 'weak': [3705, 3988], '192–328': [3709], '(ANK': [3710, 3733], '6),': [3713], '**,': [3717, 3987], 'exclude': [3721], '163.': [3730], 'Construct': [3731], '158–328': [3732], '4–6)': [3734], 'albeit': [3740], 'weaker': [3741, 3792], '1–191,': [3744], 'indicating': [3745], 'exists': [3751], 'A': [3760], 'short': [3761], 'ought': [3772], 'weakly': [3783, 4384], '3B),': [3785], 'even': [3791], 'seen': [3798, 4158], '3B).': [3804], 'tag,': [3812], 'monitor': [3818], 'localization.': [3822], 'Interestingly': [3823], '3B': [3830], 'strongly': [3832], 'expressed;': [3833], 'however,': [3834], '116–163)': [3846], 'targeted': [3848], 'mitochondria.': [3850, 3998], '3C': [3852], 'living': [3860], 'left': [3865, 4000], 'panel': [3866, 4001, 4008], 'fluorescently': [3870], 'labeled': [3871], 'mitochondria': [3872, 3902, 4012], 'same': [3875], 'panel.': [3880], 'finding': [3882, 3923], 'most': [3884], 'recombinant': [3887], 'reside': [3898], 'primarily': [3899], 'might': [3903], 'explain': [3904], 'why': [3905], 'minor': [3907], 'amounts': [3908, 4262], 'available': [3910], 'close': [3911], 'TRPV6-containing': [3913], 'membranes': [3914], 'addition,': [3921], 'observed': [3929], 'due': [3934], 'artificial': [3937], 'association': [3938, 4182], 'during': [3945], 'solubilization': [3946], 'cells.Fig.': [3948], '3Identification': [3949], 'domain.A': [3953], 'tagged': [3962], '(C-Term)': [3966], '*,': [3983], '1;': [3986], 'input.': [3989], 'C,': [3990, 4266], 'co-localization': [3993], 'fluorescence,': [4004], 'marked': [4013], 'Mito-Tracker.View': [4017], '(PPT)Analysis': [4022], '116–191—Is': [4024], '116–191': [4028, 4142], 'restored': [4047], 'forcing': [4049], 'out': [4054], 'mitochondria?': [4057], 'answer': [4059], 'questions,': [4061], 'foreign': [4063], 'protein,': [4064], 'four-transmembrane': [4066], 'inserted': [4074], '4).': [4091], 'γ1-EGFP': [4092, 4215, 4244], 'alone,': [4093], 'plasma': [4102, 4138], 'membrane': [4103, 4139], 'shown)': [4106], '4A).': [4115], 'fusing': [4117], 'indeed': [4132], 'restore': [4137], 'localization': [4140], 'and,': [4143], 'importantly,': [4145], 'γ': [4152], '4-γ1-EGFP': [4167], '4B).': [4169], 'strong': [4181], 'membranes.Fig.': [4188], '4ANK': [4189], 'fused': [4196], 'TRPV6.A,': [4213], 'does': [4219], 'TRPV6;': [4223], 'confers': [4245], 'binding.': [4247], 'Blots': [4248], 'stripped': [4250], 'reprobed': [4252], 'assure': [4260], 'immunoprecipitated': [4264], 'multimerization': [4273, 4365], 'domain.': [4274], 'extracts': [4276], '(+)': [4291], 'absence': [4293], '(–)': [4294], 'dithiothreitol,': [4298], '1.43': [4299], 'm': [4300], 'β-mercaptoethanol': [4301], 'Fusing': [4310], 'results': [4317], 'higher': [4322], 'complexes': [4325], 'apparent': [4327], 'dimers': [4328], 'trimers': [4330], '(arrows).': [4332], 'Expected': [4333], '4-GFP': [4340], 'follows:': [4343], 'monomer,': [4344], '35.2': [4345], 'kDa;': [4346, 4349], 'dimer,': [4347], '70.4': [4348], 'trimer,': [4350], '105.6': [4351], 'kDa.': [4352], 'IP,': [4353], 'immunoprecipitation;': [4354], 'WB,': [4355], 'blot.View': [4357], '(PPT)We': [4362], '4-EGFP.': [4373], 'Solubilizing': [4374], 'under': [4383, 4393], 'conditions': [4386, 4395], '(–:': [4387], '4%': [4388, 4397], '°C)': [4391], '(+:': [4396]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2036773544', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 5}, {'year': 2018, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 9}, {'year': 2013, 'cited_by_count': 6}, {'year': 2012, 'cited_by_count': 7}], 'updated_date': '2024-12-08T21:55:42.127019', 'created_date': '2016-06-24'}