Title: <title>Use of phosphorescent and fluorescent dyes for lifetime-based imaging within tissues</title>
Abstract:The use of fluorescent and phosphorescent dyes has long been established in microscopy and bio-assays for rapid and accurate biochemical spectroscopy. In this work, we explore extension of these dyes ...The use of fluorescent and phosphorescent dyes has long been established in microscopy and bio-assays for rapid and accurate biochemical spectroscopy. In this work, we explore extension of these dyes for in vivo tissue spectroscopy. Using a theoretical model for the time-dependent light propagation through scattering media, we investigate the coupling of light propagation and re-emission kinetics for florescent and phosphorescent dyes delivered deep within tissues. Our results show that the use of phosphorescent dyes can provide spectroscopy or localization of specific tissue compartments, but may be information-poor for localized spectroscopy or imaging. While re-emitted fluorescence signals contain contributions owing to both light scattering and kinetics, they do contain the necessary information for localized spectroscopy.Read More
Publication Year: 1997
Publication Date: 1997-08-18
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 4
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