Title: A method for the determination of amino acid incorporation into protein and the specific activity of tissue amino acid in small cardiac muscle samples
Abstract: A method for the determination of amino acid incorporation into protein and the specific activity of the tissue amino acid pool in cardiac muscle samples weighing as little as 1 mg is described. This technique is sufficiently sensitive to measure the specific activity of an amino acid in the picomole range and is based on the reaction of [3H]dansyl chloride with 14C-tracer amino acid. With this procedure, apparent alterations in protein synthesis noted in muscles incubated in media containing tracer amino acid of differing specific activities were normalized when the relative specific activity of tissue amino acid was considered.
Publication Year: 1973
Publication Date: 1973-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 12
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