Title: Nitrogen dioxide and nitrate nutrition effects on nitrate reductase activity and nitrate content of bean leaves
Abstract: Abstract The increase in in vivo nitrate reductase activity and nitrate content in leaves of 10-day-old bean plants ( Phaseolus vulgaris cv. Kinghorn Wax) supplied with 10 mM nitrate for up to 24 hr was compared to that of plants supplied with 0.5 μl/l NO 2 gas. Both nitrogen sources caused an increase in enzyme activity over time but only nitrate supplied leaves had an increased leaf nitrate concentration over the same time period. Inhibitors of the nitrate assimilation pathway, such as tungstate and methionine sulfoximine, inhibited the increase in nitrate reductase activity and nitrate content, an inhibition which was more apparent in the presence of nitrate than of NO 2 . The effect of albizzine was insignificant, except for nitrate reductase activity in nitrate supplied leaves. It is suggested that nitrate generated from NO 2 is metabolized more rapidly than that from nutrient nitrate.
Publication Year: 1989
Publication Date: 1989-10-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 13
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