Title: The pea seed storage protein legumin was synthesized, processed, and accumulated stably in transgenic rice endosperm
Abstract: To improve the amino acid composition of rice grain, the pea seed storage protein legumin was expressed in the endosperm of transgenic rice plants. Genomic or cDNA coding sequence of the pea legumin gene (LegA, including the 3′-downstream region) was placed under transcriptional control of either a 1.8 or 5.1 kb promoter of the rice seed storage protein glutelin gene (Gt1). The 5.1 kb promoter conferred a higher level of legumin accumulation in mature seeds than the 1.8 kb promoter, while the choice of genomic or cDNA coding sequence did not affect expression level significantly. Segregation analysis of RO seeds suggests a monogenic Mendelian inheritance, indicating a single locus or closely linked loci of gene insertion. Western blot analysis indicated that the legumin precursor is cleaved endoproteolytically into the acidic and basic subunits in the rice endosperm as is in its native pea cotyledon. We expect a significant improvement in the nutritional quality of rice seed generated by a high level expression of legumin.
Publication Year: 1997
Publication Date: 1997-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 51
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