Title: Assay for nucleoside and nucleotide binding of a potent mutagen, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone
Abstract: Deoxyadenosine, deoxyguanosine, deoxycytidine; thymidine and a dinucleotide, 2'-deoxycytidylyl-(3'-5')-2'-deoxythymidine (dCdT), were reacted with 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX). Nucleoside HPLC analysis revealed that the major products are the degradation products of MX and no stable MX-adducts were found. The same result was obtained with sodium borohydride reduction. The fluorescence spectra of deoxyguanosine-MX showed no fluorescence at neutral or alkaline pH, indicating no binding products to the N2-groups of deoxyguanosine.
Publication Year: 1991
Publication Date: 1991-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 19
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