Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2032121867', 'doi': 'https://doi.org/10.1016/j.mimet.2012.10.008', 'title': 'Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis', 'display_name': 'Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis', 'publication_year': 2012, 'publication_date': '2012-10-30', 'ids': {'openalex': 'https://openalex.org/W2032121867', 'doi': 'https://doi.org/10.1016/j.mimet.2012.10.008', 'mag': '2032121867', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/23123161'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.mimet.2012.10.008', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S24124513', 'display_name': 'Journal of Microbiological Methods', 'issn_l': '0167-7012', 'issn': ['0167-7012', '1872-8359'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5100463015', 'display_name': 'Wenbin Li', 'orcid': 'https://orcid.org/0000-0003-2801-6322'}, 'institutions': [{'id': 'https://openalex.org/I1336096307', 'display_name': 'United States Department of Agriculture', 'ror': 'https://ror.org/01na82s61', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1336096307']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Wenbin Li', 'raw_affiliation_strings': ['USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory, Beltsville, MD 20705, United States.'], 'affiliations': [{'raw_affiliation_string': 'USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory, Beltsville, MD 20705, United States.', 'institution_ids': ['https://openalex.org/I1336096307']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5088859813', 'display_name': 'D. C. Teixeira', 'orcid': 'https://orcid.org/0000-0002-5801-8049'}, 'institutions': [{'id': 'https://openalex.org/I4210086896', 'display_name': 'Fundo de Defesa da Citricultura', 'ror': 'https://ror.org/0010qfz59', 'country_code': 'BR', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210086896']}], 'countries': ['BR'], 'is_corresponding': False, 'raw_author_name': 'Diva C. Teixeira', 'raw_affiliation_strings': ['Fundecitrus, São Paulo, Brazil'], 'affiliations': [{'raw_affiliation_string': 'Fundecitrus, São Paulo, Brazil', 'institution_ids': ['https://openalex.org/I4210086896']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5061333076', 'display_name': 'John S. Hartung', 'orcid': 'https://orcid.org/0000-0001-6274-3481'}, 'institutions': [{'id': 'https://openalex.org/I1312222531', 'display_name': 'Agricultural Research Service', 'ror': 'https://ror.org/02d2m2044', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1312222531', 'https://openalex.org/I1336096307']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'John S. Hartung', 'raw_affiliation_strings': ['USDA-ARS, Beltsville, MD United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Beltsville, MD United States', 'institution_ids': ['https://openalex.org/I1312222531']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5076243476', 'display_name': 'Qi Huang', 'orcid': 'https://orcid.org/0000-0003-4278-4491'}, 'institutions': [{'id': 'https://openalex.org/I1312222531', 'display_name': 'Agricultural Research Service', 'ror': 'https://ror.org/02d2m2044', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1312222531', 'https://openalex.org/I1336096307']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Qi Huang', 'raw_affiliation_strings': ['USDA-ARS, Beltsville, MD United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Beltsville, MD United States', 'institution_ids': ['https://openalex.org/I1312222531']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5002481364', 'display_name': 'Yongping Duan', 'orcid': 'https://orcid.org/0000-0003-1775-1479'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Yongping Duan', 'raw_affiliation_strings': ['USDA-ARS, Fort Pierce, FL, United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Fort Pierce, FL, United States', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5010168602', 'display_name': 'Lijuan Zhou', 'orcid': 'https://orcid.org/0000-0002-5826-3933'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Lijuan Zhou', 'raw_affiliation_strings': ['USDA-ARS, Fort Pierce, FL, United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Fort Pierce, FL, United States', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5053491216', 'display_name': 'Jianchi Chen', 'orcid': 'https://orcid.org/0000-0003-0662-3164'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jianchi Chen', 'raw_affiliation_strings': ['USDA-ARS, Parlier, CA, United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Parlier, CA, United States', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5101833496', 'display_name': 'Hong Lin', 'orcid': 'https://orcid.org/0000-0001-6410-8676'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Hong Lin', 'raw_affiliation_strings': ['USDA-ARS, Parlier, CA, United States'], 'affiliations': [{'raw_affiliation_string': 'USDA-ARS, Parlier, CA, United States', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5072052720', 'display_name': 'S. A. Lopes', 'orcid': 'https://orcid.org/0000-0002-7417-3057'}, 'institutions': [{'id': 'https://openalex.org/I4210086896', 'display_name': 'Fundo de Defesa da Citricultura', 'ror': 'https://ror.org/0010qfz59', 'country_code': 'BR', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210086896']}], 'countries': ['BR'], 'is_corresponding': False, 'raw_author_name': 'Silvio Lopes', 'raw_affiliation_strings': ['Fundecitrus, São Paulo, Brazil'], 'affiliations': [{'raw_affiliation_string': 'Fundecitrus, São Paulo, Brazil', 'institution_ids': ['https://openalex.org/I4210086896']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5110819068', 'display_name': 'A. J. Ayres', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210086896', 'display_name': 'Fundo de Defesa da Citricultura', 'ror': 'https://ror.org/0010qfz59', 'country_code': 'BR', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210086896']}], 'countries': ['BR'], 'is_corresponding': False, 'raw_author_name': 'A. Juliano Ayres', 'raw_affiliation_strings': ['Fundecitrus, São Paulo, Brazil'], 'affiliations': [{'raw_affiliation_string': 'Fundecitrus, São Paulo, Brazil', 'institution_ids': ['https://openalex.org/I4210086896']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5111756832', 'display_name': 'Laurène Lévy', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1336096307', 'display_name': 'United States Department of Agriculture', 'ror': 'https://ror.org/01na82s61', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1336096307']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Laurene Levy', 'raw_affiliation_strings': ['USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory, Beltsville, MD 20705, United States.'], 'affiliations': [{'raw_affiliation_string': 'USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory, Beltsville, MD 20705, United States.', 'institution_ids': ['https://openalex.org/I1336096307']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 3, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2850, 'currency': 'USD', 'value_usd': 2850, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 1.258, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 49, 'citation_normalized_percentile': {'value': 0.937866, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 95, 'max': 96}, 'biblio': {'volume': '92', 'issue': '1', 'first_page': '79', 'last_page': '89'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11750', 'display_name': 'Phytoplasmas and Hemiptera pathogens', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11750', 'display_name': 'Phytoplasmas and Hemiptera pathogens', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11578', 'display_name': 'Plant Pathogenic Bacteria Studies', 'score': 0.9938, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T13069', 'display_name': 'Cocoa and Sweet Potato Agronomy', 'score': 0.993, 'subfield': {'id': 'https://openalex.org/subfields/1108', 'display_name': 'Horticulture'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/xylella-fastidiosa', 'display_name': 'Xylella fastidiosa', 'score': 0.9952744}, {'id': 'https://openalex.org/keywords/taqman', 'display_name': 'TaqMan', 'score': 0.65201294}, {'id': 'https://openalex.org/keywords/chlorosis', 'display_name': 'Chlorosis', 'score': 0.45109153}, {'id': 'https://openalex.org/keywords/fastidious-organism', 'display_name': 'Fastidious organism', 'score': 0.42616487}], 'concepts': [{'id': 'https://openalex.org/C2778410202', 'wikidata': 'https://www.wikidata.org/wiki/Q3019529', 'display_name': 'Xylella fastidiosa', 'level': 3, 'score': 0.9952744}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.83160853}, {'id': 'https://openalex.org/C143660080', 'wikidata': 'https://www.wikidata.org/wiki/Q7684883', 'display_name': 'TaqMan', 'level': 4, 'score': 0.65201294}, {'id': 'https://openalex.org/C2776411976', 'wikidata': 'https://www.wikidata.org/wiki/Q1075317', 'display_name': 'Chlorosis', 'level': 2, 'score': 0.45109153}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.44396365}, {'id': 'https://openalex.org/C2776460866', 'wikidata': 'https://www.wikidata.org/wiki/Q170065', 'display_name': 'Pathogen', 'level': 2, 'score': 0.43717855}, {'id': 'https://openalex.org/C149453125', 'wikidata': 'https://www.wikidata.org/wiki/Q5437094', 'display_name': 'Fastidious organism', 'level': 3, 'score': 0.42616487}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.41170692}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.29140705}, {'id': 'https://openalex.org/C59822182', 'wikidata': 'https://www.wikidata.org/wiki/Q441', 'display_name': 'Botany', 'level': 1, 'score': 0.22687012}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.12593412}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D001431', 'descriptor_name': 'Bacteriological Techniques', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D002957', 'descriptor_name': 'Citrus', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D010935', 'descriptor_name': 'Plant Diseases', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D060888', 'descriptor_name': 'Real-Time Polymerase Chain Reaction', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D044167', 'descriptor_name': 'Xylella', 'qualifier_ui': 'Q000145', 'qualifier_name': 'classification', 'is_major_topic': True}, {'descriptor_ui': 'D044167', 'descriptor_name': 'Xylella', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D001431', 'descriptor_name': 'Bacteriological Techniques', 'qualifier_ui': 'Q000592', 'qualifier_name': 'standards', 'is_major_topic': False}, {'descriptor_ui': 'D001431', 'descriptor_name': 'Bacteriological Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001938', 'descriptor_name': 'Brazil', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002489', 'descriptor_name': 'Central America', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002957', 'descriptor_name': 'Citrus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D015345', 'descriptor_name': 'Oligonucleotide Probes', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D015345', 'descriptor_name': 'Oligonucleotide Probes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010935', 'descriptor_name': 'Plant Diseases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012329', 'descriptor_name': 'RNA, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D012329', 'descriptor_name': 'RNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D060888', 'descriptor_name': 'Real-Time Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D060888', 'descriptor_name': 'Real-Time Polymerase Chain Reaction', 'qualifier_ui': 'Q000592', 'qualifier_name': 'standards', 'is_major_topic': False}, {'descriptor_ui': 'D012015', 'descriptor_name': 'Reference Standards', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012680', 'descriptor_name': 'Sensitivity and Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014481', 'descriptor_name': 'United States', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D044167', 'descriptor_name': 'Xylella', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D044167', 'descriptor_name': 'Xylella', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.mimet.2012.10.008', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S24124513', 'display_name': 'Journal of Microbiological Methods', 'issn_l': '0167-7012', 'issn': ['0167-7012', '1872-8359'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/23123161', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 32, 'referenced_works': ['https://openalex.org/W1626008731', 'https://openalex.org/W170392157', 'https://openalex.org/W1776630193', 'https://openalex.org/W1965137825', 'https://openalex.org/W1969270268', 'https://openalex.org/W1974751059', 'https://openalex.org/W1975021680', 'https://openalex.org/W1989811043', 'https://openalex.org/W2002881089', 'https://openalex.org/W2007591035', 'https://openalex.org/W2008940034', 'https://openalex.org/W2012801773', 'https://openalex.org/W2024041324', 'https://openalex.org/W2027513544', 'https://openalex.org/W2044187348', 'https://openalex.org/W2059428607', 'https://openalex.org/W2069128651', 'https://openalex.org/W2069606096', 'https://openalex.org/W2075942653', 'https://openalex.org/W2081009299', 'https://openalex.org/W2082091267', 'https://openalex.org/W2085847084', 'https://openalex.org/W2091478994', 'https://openalex.org/W2097213899', 'https://openalex.org/W2124346485', 'https://openalex.org/W2136019705', 'https://openalex.org/W2144003845', 'https://openalex.org/W2156339173', 'https://openalex.org/W2162072069', 'https://openalex.org/W4206195243', 'https://openalex.org/W4285719527', 'https://openalex.org/W650272533'], 'related_works': ['https://openalex.org/W4311852942', 'https://openalex.org/W4249472805', 'https://openalex.org/W2911415750', 'https://openalex.org/W2899913214', 'https://openalex.org/W2609196796', 'https://openalex.org/W2049004450', 'https://openalex.org/W2037266707', 'https://openalex.org/W2034257287', 'https://openalex.org/W2022983919', 'https://openalex.org/W1639408501'], 'abstract_inverted_index': None, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2032121867', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 7}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 12}, {'year': 2018, 'cited_by_count': 6}, {'year': 2017, 'cited_by_count': 3}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-12T20:52:50.506954', 'created_date': '2016-06-24'}