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'https://openalex.org/W2154476329', 'https://openalex.org/W2156691801', 'https://openalex.org/W2160084442', 'https://openalex.org/W2167962404', 'https://openalex.org/W2257816160', 'https://openalex.org/W2272463877', 'https://openalex.org/W2407183638', 'https://openalex.org/W2412900915', 'https://openalex.org/W328595349', 'https://openalex.org/W4247418485', 'https://openalex.org/W4285719527'], 'related_works': ['https://openalex.org/W35992800', 'https://openalex.org/W2595002348', 'https://openalex.org/W2070174251', 'https://openalex.org/W2034411310', 'https://openalex.org/W2002318349', 'https://openalex.org/W1974867906', 'https://openalex.org/W1907183830', 'https://openalex.org/W1631363513', 'https://openalex.org/W1581559601', 'https://openalex.org/W1569066968'], 'abstract_inverted_index': {'The': [0, 22, 161, 210, 232, 371, 628, 758, 869, 923, 1186, 2267, 2311, 2371, 2437, 2579, 2723, 2949, 2994, 3068, 3151, 3278, 3415, 3734, 3751, 3910, 3987, 4023], 'Treponema': [1, 211, 420], 'pallidum': [2, 205, 212, 415, 482, 641, 664, 762, 878, 1140, 1848, 1850, 2861, 3053, 3877], 'tro': [3, 213, 888, 1396, 1757, 3029, 3104, 3169, 4068], 'operon': [4, 98, 214, 308, 1099, 3105], 'encodes': [5, 215, 930, 1101, 2031], 'an': [6, 88, 216, 298, 978, 1083, 1658, 2032, 2536, 3271, 3285, 4055], 'ABC': [7, 217], 'transporter': [8, 117, 131, 156, 218, 327, 341, 366, 981, 1182, 1195, 1286, 1688, 1700, 1758, 3390], '(TroABCD),': [9, 219], 'a': [10, 92, 150, 220, 302, 360, 428, 437, 1055, 1094, 1197, 1238, 1664, 1773, 1935, 2099, 2164, 2494, 2513, 2518, 2530, 2563, 2667, 2919, 3031, 3306, 3347, 3843, 4008, 4249], 'transcriptional': [11, 221], 'repressor': [12, 222], '(TroR),': [13, 223, 1097], 'and': [14, 52, 65, 100, 112, 125, 145, 182, 224, 262, 275, 310, 322, 335, 355, 392, 466, 491, 554, 559, 636, 752, 774, 1091, 1093, 1379, 1400, 1592, 1672, 1705, 1724, 1766, 1791, 1796, 1820, 1864, 1909, 1961, 1983, 2023, 2085, 2117, 2121, 2179, 2259, 2274, 2277, 2305, 2339, 2343, 2392, 2472, 2475, 2516, 2548, 2553, 2574, 2645, 2673, 2690, 2695, 2698, 2740, 2746, 2759, 2794, 2877, 2881, 2918, 2944, 2959, 2971, 3011, 3017, 3059, 3064, 3074, 3076, 3085, 3118, 3145, 3180, 3188, 3224, 3275, 3327, 3404, 3425, 3434, 3506, 3517, 3571, 3610, 3671, 3682, 3703, 3798, 3847, 3873, 3879, 3892, 3900, 3902, 3914, 3933, 3973, 3983, 4004, 4077, 4177, 4241, 4267], 'the': [15, 27, 47, 50, 55, 68, 74, 115, 155, 179, 185, 225, 237, 257, 260, 265, 278, 284, 325, 365, 389, 395, 422, 443, 625, 659, 670, 673, 677, 864, 882, 885, 927, 931, 1098, 1102, 1138, 1165, 1171, 1180, 1189, 1193, 1277, 1280, 1285, 1315, 1342, 1390, 1395, 1405, 1683, 1687, 1695, 1707, 1719, 1734, 1756, 1769, 1794, 1805, 1813, 1830, 1840, 1903, 1947, 1994, 2070, 2091, 2110, 2180, 2198, 2366, 2386, 2398, 2447, 2451, 2479, 2488, 2539, 2559, 2575, 2589, 2671, 2677, 2728, 2744, 2760, 2804, 2820, 2842, 2846, 2865, 2890, 2895, 2922, 2926, 2930, 2986, 2991, 3027, 3040, 3045, 3079, 3103, 3112, 3160, 3168, 3293, 3339, 3409, 3697, 3700, 3725, 3746, 3760, 3780, 3793, 3875, 3905, 3997, 4028, 4042, 4046, 4087, 4099, 4151, 4156, 4188, 4200, 4206, 4218, 4310], 'essential': [16, 226, 1177], 'glycolytic': [17, 227, 1103], 'enzyme': [18, 228, 1104, 1178, 4203], 'phosphoglycerate': [19, 229], 'mutase': [20, 230], '(Gpm).': [21, 231], 'apparently': [23, 233], 'discordant': [24, 234], 'observations': [25, 235], 'that': [26, 62, 104, 175, 193, 236, 272, 314, 385, 403, 434, 740, 760, 863, 1233, 1279, 1314, 1385, 1404, 1661, 1718, 1728, 1745, 2108, 3102, 3110, 3838], 'solute': [28, 238, 932, 941, 961], 'binding': [29, 36, 239, 246, 933, 942, 962, 1378, 1391, 1535, 1607, 1679, 2190, 2245], 'protein': [30, 240, 934, 2036, 2299, 2372], '(TroA)': [31, 241], 'binds': [32, 242, 4265], 'Zn2+,': [33, 123, 243, 333, 1722], 'whereas': [34, 244, 1242, 3649], 'DNA': [35, 245, 1377, 1606, 2685, 2815, 2863, 3032, 3054, 4192], 'by': [37, 247, 624, 661, 876, 881, 1680, 1768, 1786, 1829, 1855, 2156, 2163, 2201, 2249, 2350, 2359, 2376, 2640, 2657, 2738, 2789, 2801, 2946, 3929, 4035, 4054, 4070, 4227, 4248], 'TroR': [38, 129, 248, 339, 1373, 1393, 1738, 3893], 'in': [39, 119, 133, 170, 187, 249, 329, 343, 380, 397, 494, 497, 503, 633, 658, 1237, 1247, 1527, 1545, 1590, 1593, 1691, 1702, 1781, 1799, 1804, 1824, 1912, 1993, 2466, 2538, 2662, 2700, 2841, 2925, 2985, 3159, 3201, 3249, 3267, 3289, 3305, 3355, 3696, 3731, 3753, 3777, 4155, 4316], 'vitro': [40, 250, 634, 1528, 1591], 'is': [41, 177, 251, 387, 427, 618, 669, 879, 1184, 1196, 1730, 1809], 'Mn2+-dependent,': [42, 252], 'have': [43, 253, 614, 655, 1598], 'generated': [44, 254, 2800, 2938, 3173], 'uncertainty': [45, 255], 'regarding': [46, 256], 'identities': [48, 258], 'of': [49, 54, 71, 96, 140, 143, 154, 159, 165, 168, 184, 259, 264, 281, 306, 350, 353, 364, 369, 375, 378, 394, 425, 439, 468, 630, 639, 646, 672, 676, 884, 926, 977, 1063, 1168, 1175, 1188, 1199, 1226, 1284, 1392, 1596, 1604, 1668, 1686, 1698, 1709, 1737, 1747, 1755, 1763, 1778, 1793, 1832, 1842, 1858, 1949, 2019, 2025, 2095, 2119, 2167, 2243, 2325, 2440, 2541, 2569, 2572, 2676, 2727, 2734, 2845, 2851, 2898, 2999, 3039, 3082, 3111, 3185, 3195, 3273, 3287, 3346, 3361, 3407, 3607, 3647, 3699, 3708, 3727, 3749, 3782, 3996, 4011, 4049, 4067, 4091, 4230, 4314], 'ligand(s)': [51, 261, 1191], 'co-repressor(s)': [53, 263], 'permease.': [56, 266], 'Moreover,': [57, 267], 'this': [58, 268, 1176, 1800, 1825, 2457, 2467, 2663, 3783, 4306], 'operonic': [59, 269], 'structure': [60, 270], 'suggests': [61, 174, 271, 384], 'Gpm': [63, 194, 273, 404, 1141, 1713, 1748, 3065, 3181, 3872, 3887], 'expression,': [64, 274], 'hence': [66, 276], 'glycolysis,': [67, 277], 'sole': [69, 279, 1173], 'source': [70, 280], 'ATP': [72, 282], 'for': [73, 110, 283, 320, 488, 508, 557, 632, 1145, 1192, 1374, 1533, 1775, 1906, 1946, 2072, 2188, 2348, 2357, 2643, 2792, 2979, 3329, 3430, 3560, 3568, 3612, 3790, 4199, 4211, 4223, 4233, 4238, 4244, 4256], 'bacterium,': [75, 285], 'would': [76, 199, 286, 409, 1662], 'be': [77, 201, 287, 411, 1733], 'suspended': [78, 288, 3266], 'during': [79, 289], 'TroR-mediated': [80, 147, 290, 357, 1699, 1710], 'repression.': [81, 291], 'To': [82, 292, 2184, 2665, 3025, 3867], 'resolve': [83, 293], 'these': [84, 294, 3778], 'discrepancies,': [85, 295], 'we': [86, 296, 1312, 1675, 2486], 'devised': [87, 297, 1657], 'experimental': [89, 299, 1659], 'strategy': [90, 300, 1660], 'permitting': [91, 301], 'more': [93, 303, 555, 1665], 'direct': [94, 304, 1666], 'assessment': [95, 305, 1667], 'Tro': [97, 116, 307, 326, 1181, 1194, 1669, 1770], 'function': [99, 309, 1671], 'regulation.': [101, 311, 1673], 'We': [102, 312, 1655, 1742], 'report': [103, 313], '(i)': [105, 315, 1677, 4167], 'apo-TroA': [106, 316], 'has': [107, 317, 642, 1142, 2037, 3806], 'identical': [108, 318, 1602], 'affinities': [109, 319], 'Zn2+': [111, 141, 166, 176, 208, 321, 351, 376, 386, 418, 558, 1234, 1318, 1536, 1729, 2335, 2349, 3348, 3410, 3515, 4266, 4278, 4315], 'Mn2+;': [113, 144, 323, 354], '(ii)': [114, 324, 1682, 4172], 'expressed': [118, 328, 1544, 1690], 'Escherichia': [120, 330, 1230], 'coli': [121, 135, 331, 345, 1231, 1547, 1898, 2963, 3001, 3190, 3351, 3363, 3484, 3508, 3835, 3858, 3926, 4275, 4318], 'imports': [122, 332], 'Mn2+,': [124, 334, 1723], 'possibly': [126, 336, 1725], 'iron;': [127, 337], '(iii)': [128, 338, 1694, 4178], 'represses': [130, 340], 'expression': [132, 195, 342, 405, 1746, 3906], 'E.': [134, 344, 700, 1015, 1452, 1472, 1546, 1572, 1703, 1897, 1950, 2962, 3000, 3189, 3350, 3362, 3483, 3507, 3813, 3834, 3857, 3925, 3971, 4274, 4317], 'at': [136, 346, 764, 1812, 2365, 2743, 3148, 3302, 3332, 3419, 3589, 3722, 4253], 'significantly': [137, 347, 2462], 'lower': [138, 348], 'concentrations': [139, 164, 349, 374, 2373, 2390, 3406], 'than': [142, 158, 167, 352, 368, 377, 1538], '(iv)': [146, 356, 1706], 'repression': [148, 358, 1701, 1711, 4044], 'causes': [149, 359], 'disproportionately': [151, 361], 'greater': [152, 362], 'down-regulation': [153, 363], 'genes': [157, 367, 742, 1183, 2856, 2924, 2998, 3881], 'gpm.': [160, 370], 'much': [162, 372], 'higher': [163, 373], 'Mn2+': [169, 379, 560, 1387, 1408, 2333, 2358, 4268], 'human': [171, 381, 432], 'body': [172, 382], 'fluids': [173, 383], 'both': [178, 388, 1520, 2528], 'primary': [180, 390, 1540, 1735], 'substrate': [181, 391, 1541, 4032], 'co-repressor': [183, 393], 'permease': [186, 396, 1316, 1406, 1670, 1720, 1771], 'vivo.': [188, 398], 'Our': [189, 399, 1224, 1715], 'data': [190, 400], 'also': [191, 401, 1100, 1743, 2067], 'indicate': [192, 402, 1717], 'and,': [196, 406, 1785], 'therefore,': [197, 407], 'glycolysis': [198, 408], 'not': [200, 410, 1599, 1750, 2460], 'abrogated': [202, 412], 'when': [203, 413, 1542, 1689], 'T.': [204, 414, 481, 602, 640, 663, 718, 761, 777, 877, 1139, 1501, 1847, 2860, 3052, 3876, 3969, 4111], 'encounters': [206, 416], 'high': [207, 417, 4312], 'levels.': [209, 419], 'pallidum,': [421, 3970], 'causative': [423], 'agent': [424], 'syphilis,': [426], 'noncultivable': [429], 'sexually': [430], 'transmitted': [431], 'pathogen': [433], 'disseminates': [435], 'from': [436, 1229, 1519, 1526, 2197, 2463, 2493, 2584, 2634, 2682, 2783, 2811, 2859, 2939, 2961, 3035, 3051, 3591, 3644, 3658, 3736, 3862, 3923, 4273], 'site': [438, 1816, 2897], 'inoculation,': [440], 'usually': [441], 'within': [442], 'genital': [444], 'area,': [445], 'to': [446, 499, 872, 1054, 1081, 1179, 1394, 1402, 1732, 1753, 2098, 2271, 2318, 2394, 2444, 2450, 2754, 2953, 3044, 3109, 3270, 3284, 3573, 3594, 3640, 3654, 3738, 3744, 3792, 3849, 3935, 3985, 3993, 4037], 'diverse': [447], 'organs': [448], 'where': [449, 2407], 'it': [450], 'can': [451], 'establish': [452], 'persistent,': [453], 'even': [454], 'lifelong,': [455], 'infection': [456], '(1Tramont': [457], 'E.C.': [458], 'Mandell': [459], 'G.L.': [460], 'Bennett': [461], 'J.E.': [462, 1150, 1349], 'Dolin': [463], 'R.': [464, 604, 690, 696, 783, 787, 1288, 4117, 4121], 'Principles': [465], 'Practice': [467], 'Infectious': [469], 'Diseases.': [470], 'Churchill': [471], 'Livingtone,': [472], 'Inc.,': [473], 'New': [474, 1860], 'York2000:': [475], '2474-2490Google': [476], 'Scholar).': [477, 922, 1078, 1136, 1223, 1275, 1340, 1517, 1588, 1654, 1893, 2017, 2064, 2151, 2241, 2436, 2631, 2780, 3482, 3635, 3829, 3968, 4304], 'Like': [478], 'all': [479, 2298, 2571, 3245, 3513, 3650], 'pathogens,': [480], 'presumably': [483], 'requires': [484], 'sequestered': [485], 'transition': [486, 1064], 'metals': [487, 1146, 1244, 2194], 'vital': [489], 'structural': [490], 'catalytic': [492], 'functions': [493], 'proteins.': [495], 'Nevertheless,': [496], 'contrast': [498], 'other': [500, 1243, 1343], 'pathogenic': [501], 'bacteria': [502], 'which': [504, 662, 738, 1808, 2030, 2089, 2171, 2526, 2557, 2611, 3166, 3336, 3511, 3920], 'specialized': [505], 'uptake': [506, 1765, 3516], 'systems': [507], 'iron': [509, 1726], '(2Payne': [510], 'S.M.': [511, 1505], 'Trends': [512], 'Microbiol.': [513, 531, 545, 564, 1070, 1204, 1292, 1303, 1412, 1478, 1491, 1614, 2600, 2623, 2712, 2772, 3236, 3474, 3497], '1993;': [514, 1294, 1581], '1:': [515], '66-69Abstract': [516], 'Full': [517, 806, 808, 830, 832, 1645, 1647, 4140, 4142], 'Text': [518, 807, 809, 831, 833, 1646, 1648, 4141, 4143], 'PDF': [519, 810, 834, 1649, 4144], 'PubMed': [520, 535, 549, 568, 579, 592, 611, 733, 811, 835, 857, 918, 1007, 1029, 1048, 1074, 1132, 1160, 1208, 1219, 1273, 1297, 1307, 1336, 1366, 1416, 1427, 1440, 1460, 1482, 1495, 1513, 1564, 1584, 1618, 1632, 1650, 1889, 2013, 2062, 2149, 2237, 2434, 2507, 2604, 2627, 2716, 2776, 2836, 2913, 3096, 3219, 3240, 3381, 3460, 3478, 3501, 3535, 3631, 3827, 3964, 4145, 4302, 4327], 'Scopus': [521, 536, 550, 569, 580, 593, 734, 812, 836, 858, 919, 1008, 1049, 1075, 1133, 1161, 1209, 1220, 1308, 1337, 1367, 1417, 1428, 1441, 1461, 1483, 1496, 1514, 1565, 1585, 1619, 1651, 1890, 2014, 2238, 2508, 2605, 2628, 2717, 2777, 2837, 2914, 3097, 3220, 3241, 3382, 3461, 3479, 3502, 3536, 3632, 3965, 4146, 4328], '(169)': [522], 'Google': [523, 538, 552, 571, 582, 595, 612, 736, 814, 838, 860, 921, 1010, 1030, 1051, 1077, 1135, 1163, 1211, 1222, 1274, 1298, 1310, 1339, 1369, 1419, 1430, 1443, 1463, 1485, 1498, 1516, 1567, 1587, 1621, 1633, 1653, 1892, 2016, 2063, 2150, 2240, 2435, 2510, 2607, 2630, 2719, 2779, 2839, 2916, 3099, 3222, 3243, 3384, 3463, 3481, 3504, 3538, 3634, 3828, 3967, 4148, 4303, 4330], 'Scholar,': [524, 539, 572, 583, 596, 815, 839, 1011, 1031, 1212, 1299, 1420, 1431, 1444, 1464, 1486, 1499, 1568, 1622, 1634, 3464], '3Ratledge': [525], 'C.': [526, 724, 779, 785, 1019, 1609, 3375, 3529, 3817, 4113, 4119], 'Dover': [527, 1610], 'L.G.': [528, 1611], 'Annu.': [529, 1612], 'Rev.': [530, 1293, 1613], '2000;': [532, 803, 827, 1510, 1615, 1642, 3475, 3498, 4137], '54:': [533, 1616], '881-941Crossref': [534, 1617], '(1141)': [537, 1620], '4Vasil': [540], 'M.L.': [541, 1878, 2499, 3468, 3491, 3953], 'Ochsner': [542], 'U.A.': [543], 'Mol.': [544, 1477, 2599, 2622, 2711, 2771, 3235, 3473, 3496], '1999;': [546, 1045, 1270, 1333, 1363, 2059, 2146, 2431, 2601, 2624, 2773, 3237, 4299], '34:': [547], '399-413Crossref': [548], '(282)': [551], 'Scholar),': [553, 613, 737, 861, 1164, 1311, 1370, 2608, 2720, 2917, 3539], 'recently': [556], '(5Claverys': [561, 1067, 1201, 1409], 'J.-P.': [562, 1068, 1202, 1410], 'Res.': [563, 1069, 1203, 1302, 1411], '2001;': [565, 576, 589, 1071, 1157, 1205, 1216, 1304, 1413, 1424, 1437, 1457, 1492, 1886, 2010, 3216, 3961, 4324], '152:': [566, 1072, 1206, 1305, 1414], '231-243Crossref': [567, 1073, 1207, 1415], '(134)': [570, 1076, 1210, 1418], '6Hantke': [573, 1213, 1421], 'K.': [574, 1214, 1422, 1488, 1638], 'Biometals.': [575, 1215, 1423], '14:': [577, 1217, 1425], '239-249Crossref': [578, 1218, 1426], '(146)': [581, 1221, 1429], '7Jakubovics': [584, 1432], 'N.S.': [585, 1433], 'Jenkinson': [586, 1434], 'H.F.': [587, 1435], 'Microbiology.': [588, 1436], '147:': [590, 1438], '1709-1718Crossref': [591, 1439], '(199)': [594, 1442], '8Bhattacharyya-Pakrasi': [597], 'M.': [598, 692, 716, 817, 821], 'Pakrasi': [599], 'H.B.': [600], 'Ogawa': [601], 'Aurora': [603], 'Biochem.': [605, 1579], 'Soc.': [606], 'Trans.': [607], '2002;': [608, 854, 1479, 2234, 3378, 3457, 3532, 3628], '30:': [609], '768-770Crossref': [610], 'been': [615, 2038, 2114, 3807, 3840], 'identified,': [616], 'there': [617], 'little': [619], 'information': [620], 'about': [621], 'metal': [622, 650, 874, 1065, 1190, 1678, 1696, 1764, 1783, 1925, 2189, 2244, 2388, 4043], 'acquisition': [623], 'syphilis': [626, 1788], 'spirochete.': [627], 'lack': [629], 'techniques': [631], 'cultivation': [635], 'genetic': [637], 'manipulation': [638], 'greatly': [643], 'hindered': [644], 'investigation': [645], 'treponemal': [647, 1782, 2923], 'physiology,': [648], 'including': [649], 'metabolism.': [651], 'Two': [652], 'recent': [653], 'developments': [654], 'spawned': [656], 'interest': [657], 'processes': [660], 'acquires': [665], 'trace': [666, 1248], 'metals.': [667], 'One': [668], 'availability': [671], 'genomic': [674, 2496, 2976], 'sequence': [675, 2888, 2947], 'spirochete': [678], '(9Fraser': [679], 'C.M.': [680], 'Norris': [681, 911, 1000, 1125, 1352], 'S.J.': [682, 912, 1001, 1126, 1353, 1454, 1468, 1476], 'Weinstock': [683, 909, 998, 1123], 'G.M.': [684, 910, 999, 1124], 'White': [685, 1862], 'O.': [686], 'Sutton': [687], 'G.G.': [688], 'Dodson': [689], 'Gwinn': [691], 'Hickey': [693], 'E.K.': [694], 'Clayton': [695], 'Ketchum': [697], 'K.A.': [698], 'Sodergren': [699], 'Hardham': [701, 1350], 'J.M.': [702, 894, 983, 1108, 1351, 1578, 3452, 3623], 'McLeod': [703], 'M.P.': [704], 'Salzberg': [705], 'S.': [706, 1148, 1361, 1474, 1999, 2968, 3205], 'Peterson': [707], 'J.': [708, 800, 824, 1024, 1155, 1268, 1576, 1580, 1627, 1639, 1883, 2057, 2144, 2232, 2429, 2907, 2908, 3376, 3455, 3530, 3626, 3822, 3958, 4134, 4297], 'Khalak': [709], 'H.': [710, 1450], 'Richardson': [711], 'D.': [712, 819, 1017, 1259, 2048, 2135, 2420, 2903, 3815, 4288], 'Howell': [713, 903, 992, 1117], 'J.K.': [714, 904, 993, 1118], 'Chidambaram': [715], 'Utterback': [717], 'McDonald': [719], 'L.': [720], 'Artiach': [721], 'P.': [722], 'Bowman': [723], 'Cotton': [725], 'M.D.': [726, 3369, 3523], 'Venter': [727], 'J.C.': [728], 'Science.': [729, 4323], '1998;': [730, 1629], '281:': [731], '375-388Crossref': [732], '(829)': [735], 'reveals': [739], 'many': [741], 'encoding': [743, 1082], 'well': [744, 2534], 'characterized': [745], 'iron-containing': [746], 'proteins': [747, 2200], '(e.g.': [748], 'cytochromes,': [749], 'superoxide': [750, 771], 'dismutase,': [751], 'tricarboxylic': [753], 'acid': [754, 2169, 2378, 3931], 'metalloenzymes)': [755], 'are': [756, 1531, 1802, 2983, 2990], 'lacking.': [757], 'finding': [759], 'expresses': [763], 'least': [765], 'two': [766, 1086], 'experimentally': [767], 'confirmed': [768, 2107, 2375, 2656, 2945], 'iron-binding': [769], 'proteins,': [770, 3919], 'reductase': [772], '(neelaredoxin)': [773], 'rubredoxin,': [775], '(10Jovanovíc': [776, 4110], 'Ascenso': [778, 4112], 'Hazlett': [780, 2222, 4114], 'K.R.O.': [781, 841, 1870, 2223, 3945, 4115], 'Sikkink': [782, 844, 4116], 'Krebs': [784, 4118], 'Litwiller': [786, 4120], 'Benson': [788, 4122], 'L.M.': [789, 2901, 4123], 'Moura': [790, 792, 4124, 4126], 'I.': [791, 1574, 4125], 'J.J.G.': [793, 4127], 'Radolf': [794, 850, 907, 996, 1022, 1038, 1121, 1266, 1326, 1881, 2055, 2142, 2228, 2427, 3820, 3956, 4128, 4295], 'J.D.': [795, 851, 908, 997, 1023, 1039, 1122, 1267, 1327, 1626, 1882, 2005, 2056, 2143, 2229, 2428, 2594, 3211, 3821, 3957, 4129, 4296], 'Huynh': [796, 4130], 'B.H.': [797, 4131], 'Rusnak': [798, 848, 4132], 'F.': [799, 847, 849, 4133], 'Biol.': [801, 825, 1044, 1332, 1640, 2502, 4135], 'Chem.': [802, 826, 1641, 4136], '275:': [804, 828, 1643, 4138], '28439-28448Abstract': [805, 4139], '(94)': [813, 4147], '11Lombard': [816], 'Touati': [818], 'Fontecave': [820], 'Niviere': [822], 'V.': [823], '27021-27026Abstract': [829], '(131)': [837, 3462, 3633], '12Hazlett': [840], 'Cox': [842, 1020, 1469, 1875, 3818, 3950], 'D.L.': [843, 1021, 1876, 3819, 3951], 'R.A.': [845, 1558], "Auch'ere": [846], 'Methods': [852], 'Enzymol.': [853], '353:': [855], '140-156Crossref': [856], '(18)': [859], 'argues': [862], 'bacterium': [865], 'does': [866], 'require': [867], 'iron.': [868], 'second': [870, 2165], 'impetus': [871], 'study': [873], 'utilization': [875], 'provided': [880], 'discovery': [883], 'six': [886], 'gene': [887, 925, 2679, 2806], '(transport-related': [889], 'operon)': [890], '(Fig.': [891], '1)': [892], '(13Hardham': [893, 982, 1107], 'Stamm': [895, 984, 1109], 'L.V.': [896, 985, 1110], 'Porcella': [897, 986, 1111], 'S.F.': [898, 987, 1112], 'Frye': [899, 988, 1113], 'J.G.': [900, 989, 1114], 'Barnes': [901, 990, 1115], 'N.Y.': [902, 991, 1116], 'Mueller': [905, 994, 1119], 'S.L.': [906, 995, 1120], 'Gene': [913, 1002, 1127, 2831, 3091], '(Amst.).': [914, 1003, 1128, 2832, 3092], '1997;': [915, 1004, 1026, 1129, 2713, 3824], '197:': [916, 1005, 1130], '47-64Crossref': [917, 1006, 1131], '(62)': [920, 1009, 1134, 1891, 3966], 'first': [924], 'operon,': [928, 3030], 'troA,': [929], '(SBP)': [935], '1The': [936, 956], 'abbreviations': [937, 957], 'used': [938, 958, 1798, 2661, 2753, 2952, 3639, 3653, 3743, 3776, 3934], 'are:': [939, 959], 'SBP,': [940, 960], 'protein;': [943, 963], 'HRMM,': [944, 964, 3674], 'Hazlett-Radolf': [945, 965], 'minimal': [946, 966, 1938, 1941], 'medium;': [947, 967], 'PIPES,': [948, 968, 1985], 'piperazine-N,N′-bis(2-ethanesulfonic': [949, 969], 'acid);': [950, 970], 'RT,': [951, 971, 4187], 'reverse': [952, 972], 'transcription;': [953, 973], 'TPEN,': [954, 974], 'N,N,N′,N′-tetrakis(2-pyridyl-methyl)ethylenediamine.': [955, 975], 'component': [976], 'ATP-binding': [979], 'cassette': [980], '14Akins': [1012], 'D.R.': [1013, 3811], 'Robinson': [1014, 3812], 'Shevchenko': [1016, 1258, 2047, 2134, 2419, 3814, 4287], 'Elkins': [1018, 3816], 'Bacteriol.': [1025, 1156, 1269, 1628, 2058, 2145, 2233, 2430, 2909, 3377, 3456, 3531, 3627, 3823, 4298], '179:': [1027, 3825], '5076-5086Crossref': [1028, 3826], '15Lee': [1032], 'Y.H.': [1033, 1255, 1321, 2044, 2131, 2219, 2416, 4284], 'Deka': [1034, 1322, 2224], 'R.K.': [1035, 1253, 1323, 2042, 2129, 2225, 2414, 4282], 'Norgard': [1036, 1264, 1324, 2053, 2140, 2226, 2425, 4293], 'M.V.': [1037, 1265, 1325, 2054, 2141, 2227, 2426, 4294], 'Hasemann': [1040, 1262, 1328, 2051, 2138, 2230, 2423, 4291], 'C.A.': [1041, 1261, 1263, 1329, 2050, 2052, 2137, 2139, 2231, 2422, 2424, 4290, 4292], 'Nat.': [1042, 1330], 'Struct.': [1043, 1331], '6:': [1046, 1334], '628-633Crossref': [1047, 1335], '(139)': [1050, 1338], 'Scholar)': [1052, 2511, 2840, 3100, 3223, 3385, 3505, 4149, 4331], 'belonging': [1053], 'newly': [1056], 'described': [1057, 1823, 1867, 2039, 2127, 2942, 3229, 3446, 3616, 3809, 3943, 4109], 'cluster': [1058], '(cluster': [1059], 'nine': [1060], 'or': [1061, 1249, 1934, 2334, 2356, 3133, 3297, 3321, 3389, 3401, 3413, 3544, 3581, 3685, 3715, 4000], 'C9)': [1062], 'permeases': [1066, 1089], 'In': [1079, 4041, 4184], 'addition': [1080], 'ATPase': [1084], '(TroB),': [1085], 'cytoplasmic': [1087], 'membrane': [1088], '(TroC': [1090], 'TroD),': [1092], 'DtxR-like': [1095], 'metalloregulator': [1096], 'phosphoglyceromutase': [1105], '(Gpm)': [1106], 'Because': [1137], 'no': [1143], 'requirement': [1144], '(16Benoit': [1147], 'Posey': [1149, 1345], 'Chenoweth': [1151], 'M.R.': [1152, 2221, 2708], 'Gherardini': [1153, 1354], 'F.C.': [1154, 1355], '183:': [1158], '4702-4708Crossref': [1159], '(7)': [1162], 'physiological': [1166, 1740], 'benefit': [1167], 'transcriptionally': [1169], 'linking': [1170], "spirochete's": [1172], 'copy': [1174], 'unclear.': [1185], 'identity': [1187], 'subject': [1198], 'controversy': [1200], 'analysis': [1225, 1777, 2106, 3194, 3345], 'TroA': [1227, 2020, 2035, 2082, 2086, 2874, 3177, 3805], 'purified': [1228, 2199, 3922, 4272], 'showed': [1232], 'was': [1235, 1749, 1853, 1902, 1910, 1944, 2247, 2442, 2544, 2586, 2612, 2655, 2660, 2752, 2799, 2823, 2892, 2934, 2937, 3049, 3070, 3106, 3154, 3171, 3199, 3310, 3342, 3353, 3427, 3775, 3831, 3860, 4197, 4215, 4260], 'present': [1236, 1246], '1:1': [1239], 'molar': [1240], 'ratio,': [1241], 'were': [1245, 1827, 1991, 2123, 2154, 2195, 2269, 2316, 2346, 2374, 2383, 2638, 2680, 2787, 2857, 3003, 3123, 3247, 3265, 3282, 3325, 3392, 3417, 3442, 3540, 3548, 3597, 3638, 3652, 3665, 3694, 3729, 3742, 3757, 3769, 3882, 3921, 3977, 3989, 4025, 4052, 4072, 4089, 4096, 4165], 'undetectable': [1250], 'quantities': [1251], '(17Deka': [1252, 2041, 2128, 2413, 4281], 'Lee': [1254, 2043, 2130, 2415, 4283], 'Hagman': [1256, 2045, 2132, 2417, 4285], 'K.E.': [1257, 2046, 2133, 2418, 4286], 'Lingwood': [1260, 2049, 2136, 2421, 4289], '181:': [1271, 2060, 2147, 2432, 4300], '4420-4423Crossref': [1272, 2061, 2148, 2433, 4301], 'On': [1276, 1341], 'assumption': [1278], 'SBP': [1281], 'determines': [1282], 'specificity': [1283, 1697], '(18Tam': [1287], 'Saier': [1289], 'Jr.,': [1290, 1553], 'M.H.': [1291], '57:': [1295], '320-346Crossref': [1296], '19Higgins': [1300], 'C.F.': [1301], '205-210Crossref': [1306], '(464)': [1309], 'proposed': [1313], 'mediates': [1317], 'influx': [1319], '(15Lee': [1320], 'hand,': [1344], 'et': [1346], 'al.': [1347], '(20Posey': [1348], 'Proc.': [1356], 'Natl.': [1357], 'Acad.': [1358], 'Sci.': [1359], 'U.': [1360], 'A.': [1362, 1624, 3450, 3621], '96:': [1364], '10887-10892Crossref': [1365], '(91)': [1368], 'using': [1371, 2397, 2512, 2686, 2816, 2864, 3005, 3055, 3156, 3174, 3759, 3884, 4027, 4074, 4150, 4217], 'recombinant': [1372, 1907], 'mobility': [1375], 'shift': [1376], 'DNase': [1380, 4082], 'I': [1381], 'footprint': [1382], 'assays,': [1383, 3779], 'reported': [1384], 'only': [1386, 2109], 'could': [1388, 4308], 'promote': [1389], 'promoter,': [1397, 3115, 3170], 'leading': [1398], 'them': [1399], 'others': [1401], 'conclude': [1403], 'transports': [1407, 1721], '21Horsburgh': [1445], 'M.J.': [1446, 1466, 1880, 2905, 3955], 'Clements': [1447], 'M.O.': [1448], 'Crossley': [1449], 'Ingham': [1451, 1471], 'Foster': [1453, 1475], 'Infect.': [1455, 1508, 2008, 3214], 'Immun.': [1456, 1509, 2009, 3215], '69:': [1458, 2011, 3217], '3744-3754Crossref': [1459], '(260)': [1462], '22Horsburgh': [1465], 'Wharton': [1467], 'A.G.': [1470], 'Peacock': [1473], '44:': [1480], '1269-1286Crossref': [1481], '(190)': [1484], '23Hantke': [1487], 'Curr.': [1489], 'Opin.': [1490], '4:': [1493, 2505], '172-177Crossref': [1494], '(566)': [1497], '24Kitten': [1500], 'Munro': [1502], 'C.L.': [1503], 'Michalek': [1504], 'Macrina': [1506], 'F.L.': [1507], '68:': [1511], '4441-4451Crossref': [1512], '(73)': [1515], 'Conclusions': [1518], 'studies': [1521, 1595], 'are,': [1522], 'however,': [1523], 'essentially': [1524], 'extrapolations': [1525], 'data.': [1529], 'There': [1530], 'precedents': [1532], 'metalloproteins': [1534], 'rather': [1537], 'their': [1539], 'heterologously': [1543], '(25Eidsness': [1548], 'M.K.': [1549], "O'Dell": [1550], 'S.E.': [1551], 'Kurtz': [1552], 'D.M.': [1554], 'Robson': [1555], 'R.L.': [1556, 3373, 3527], 'Scott': [1557], 'Protein.': [1559], 'Eng.': [1560], '1992;': [1561], '5:': [1562], '367-371Crossref': [1563], '(56)': [1566], '26Petillot': [1569], 'Y.': [1570, 2469, 2480, 2615, 2683, 2756, 2764, 2812, 2965, 3186, 3196, 3256, 3974], 'Forest': [1571], 'Mathieu': [1573], 'Meyer': [1575], 'Moulis': [1577], '296:': [1582], '657-661Crossref': [1583], '(51)': [1586], 'Similarly,': [1589], 'vivo': [1594], 'metalloregulators': [1597], 'always': [1600], 'yielded': [1601], 'profiles': [1603], 'metal-dependent': [1605], '(3Ratledge': [1608], '27Gaballa': [1623], 'Helmann': [1625], '180:': [1630], '5815-5821Crossref': [1631], '28Patzer': [1635], 'S.I.': [1636], 'Hantke': [1637], '24321-24332Abstract': [1644], '(166)': [1652], 'therefore': [1656], 'permit': [1663], 'Here': [1674], 'examined': [1676], 'apo-TroA;': [1681], 'functional': [1684], 'capacity': [1685, 2246], 'surrogate': [1692], 'hosts;': [1693], 'coli;': [1704], 'effect': [1708], 'on': [1712, 3125, 3260, 3599, 3673, 3842, 3979], 'expression.': [1714], 'findings': [1716], 'but': [1727], 'likely': [1731, 3788], 'activator': [1736], 'under': [1739, 1839, 3785], 'conditions.': [1741], 'found': [1744], 'tightly': [1751], 'linked': [1752], 'transcription': [1754], 'genes.': [1759], 'This': [1760, 2065], 'revised': [1761], 'understanding': [1762], 'regulation': [1767], 'provides': [1772], 'foundation': [1774], 'further': [1776], 'its': [1779, 2808], 'role': [1780], 'metabolism': [1784], 'extension,': [1787], 'pathogenesis.': [1789], 'Primers': [1790], 'Plasmids—Descriptions': [1792], 'primers': [1795, 2081, 2872, 3006, 3060, 3157, 3175, 4153], 'plasmids': [1797, 3083, 3391, 3912], 'work': [1801, 1826], 'presented': [1803, 2465, 2984], 'supplemental': [1806, 2987, 3161, 4157], 'table,': [1807, 2988], 'available': [1810], 'online': [1811], 'JBC': [1814], 'web': [1815], '(www.jbc.org).': [1817], 'Bacterial': [1818], 'Strains': [1819], 'Media—Animal': [1821], 'protocols': [1822], 'approved': [1828], 'University': [1831], 'Connecticut': [1833], 'Health': [1834], 'Center': [1835], 'Animal': [1836, 1843], 'Care': [1837], 'Committee': [1838], 'auspices': [1841], 'Welfare': [1844], 'Assurance': [1845], 'A3471-01.': [1846], 'subspecies': [1849], '(Nichols-Farmington': [1851], 'strain)': [1852], 'propagated': [1854], 'intratesticular': [1856], 'inoculation': [1857], 'adult': [1859], 'Zealand': [1861], 'rabbits': [1863], 'harvested': [1865], 'as': [1866, 2069, 2125, 2279, 2529, 2533, 2535, 2929, 2941, 2989, 3228, 3444, 3615, 3941, 4107, 4262], 'previously': [1868, 2040, 2126, 3230, 3445, 3617, 3808, 3942, 4108], '(29Hazlett': [1869, 3944], 'Sellati': [1871, 3946], 'T.J.': [1872, 3947], 'Nguyen': [1873, 3948], 'T.T.': [1874, 3949], 'Clawson': [1877, 3952], 'Caimano': [1879, 3954], 'Exp.': [1884, 3959], 'Med.': [1885, 3960], '193:': [1887, 3962], '1015-1026Crossref': [1888, 3963], 'Unless': [1894], 'otherwise': [1895], 'indicated,': [1896], 'strain': [1899, 1905, 3352, 3485, 3509], 'TOP10F′': [1900], '(Invitrogen)': [1901, 2161, 4076], 'recipient': [1904], 'constructs': [1908], 'grown': [1911, 1992, 3146, 3276, 3559], 'LB': [1913], 'medium': [1914, 1929, 1939, 1942, 1953, 1996, 3269, 3396], 'with': [1915, 1923, 2080, 2158, 2308, 2332, 2546, 2551, 2562, 2693, 2807, 3072, 3254, 3296, 3422, 3542, 3564, 3604, 3705, 3833, 3898, 3991, 4007, 4079, 4098, 4169, 4174, 4180, 4269], 'appropriate': [1916, 3398, 3998], 'antibiotic': [1917], 'supplementation.': [1918], 'For': [1919, 3252, 4159], 'experiments': [1920, 3253, 3741], 'requiring': [1921], 'media': [1922], 'reduced': [1924], 'contents,': [1926], 'either': [1927, 3387, 3408, 3683], 'M9': [1928, 1952, 1956, 3126], '(Becton': [1930, 3692], 'Dickinson,': [1931], 'Sparks,': [1932], 'MD)': [1933], 'specially': [1936], 'formulated': [1937], '(Hazlett-Radolf': [1940], '(HRMM))': [1943], 'utilized': [1945], 'growth': [1948, 3337, 3426], 'coli.': [1951], 'contains': [1954, 1965, 4276], '1×': [1955], 'salts,': [1957], '0.2%': [1958, 1968, 1971, 1973, 3299, 3402, 3686], 'casamino': [1959, 1969], 'acids,': [1960, 1970], '0.4%': [1962, 1966, 3551], 'glucose.': [1963], 'HRMM': [1964, 3356, 3359, 3670], 'glucose,': [1967], 'ATP,': [1972], 'ammonium': [1974], 'sulfate,': [1975, 1979], '1': [1976, 2412, 3712, 3716], 'mm': [1977, 2214, 3139, 3566], 'magnesium': [1978], '9': [1980], 'μm': [1981, 2276, 3319, 3323], 'FeSO4,': [1982], '1.67%': [1984], 'pH': [1986], '6.5.': [1987], 'Yersinia': [1988], 'pestis': [1989, 2470, 2481, 2616, 2684, 2757, 2765, 2813, 2966, 3187, 3197, 3257, 3975], 'strains': [1990, 3198, 3258], 'defined': [1995], 'PMH2': [1997, 3202, 3268, 3291], '(30Gong': [1998, 3204], 'Bearden': [2000, 3206], 'S.W.': [2001, 2619, 2768, 3207, 3232], 'Geoffroy': [2002, 3208], 'V.A.': [2003, 3209], 'Fetherston': [2004, 3210], 'Perry': [2006, 2500, 2597, 2620, 2769, 3212, 3233], 'R.D.': [2007, 2501, 2598, 2621, 2770, 3213, 3234], '2829-2837Crossref': [2012, 3218], '(98)': [2015, 3221], 'Generation': [2018], 'apo': [2021], 'Proteins': [2022], 'Determination': [2024], 'Dissociation': [2026], 'Constants—The': [2027], 'plasmid': [2028, 2066, 2484, 2532, 2920], '(pHTa:TroA),': [2029], 'isopropyl-β-d-thiogalactopyranoside-inducible': [2033, 3917], 'polyhistidine-tagged': [2034, 3918], 'served': [2068, 2527], 'template': [2071, 4182], 'site-directed': [2073], 'mutagenesis': [2074], '(QuikChange,': [2075], 'Stratagene,': [2076], 'La': [2077], 'Jolla,': [2078], 'CA)': [2079], 'H199E': [2083, 2087], '5′': [2084, 2672, 2745, 2875, 3062, 3178, 3888, 3894], '3′,': [2088], 'changed': [2090], 'H': [2092], 'codon': [2093, 2101, 3043, 3048], '(CAT)': [2094], 'residue': [2096], '199': [2097], 'E': [2100], '(GAA),': [2102], 'generating': [2103, 2524, 2555, 2609, 3023], 'pHTa:TroA(H199E).': [2104], 'Sequence': [2105], 'desired': [2111], 'mutation': [2112], 'had': [2113, 3839], 'introduced.': [2115], 'Expression': [2116], 'purification': [2118], 'His-TroA': [2120], 'His-TroA(H199E)': [2122], 'performed': [2124, 4097, 4216, 4261], 'His': [2152, 2174], 'tags': [2153], 'removed': [2155, 2196], 'digestion': [2157], 'TEV': [2159, 2182], 'protease': [2160], 'followed': [2162, 4034, 4226, 4247], 'round': [2166], 'nickel-nitrilotriacetic': [2168, 3930], 'chromatography,': [2170], 'captures': [2172], 'free': [2173, 2387, 4090], 'tag,': [2175], 'uncleaved': [2176], 'fusion': [2177], 'protein,': [2178], 'His-tagged': [2181], 'protease.': [2183], 'generate': [2185, 2478, 2666], 'apo-proteins': [2186, 2268], 'suitable': [2187], 'studies,': [2191, 4045], 'endogenously': [2192], 'bound': [2193], 'overnight': [2202, 3147, 3720], 'dialysis': [2203, 2251, 2287, 2312], 'against': [2204, 2252, 2385, 3804, 3856, 3871], '0.1': [2205, 2253, 2260, 2326, 3138, 3274, 3288, 3679], 'm': [2206, 2254, 2261, 2327, 3710, 3713, 3717], 'sodium': [2207, 2255, 2262, 2328], 'acetate': [2208, 2256, 2263, 2329, 2336], 'buffer': [2209], '(pH': [2210, 2257, 2264, 2330], '4.6)': [2211, 2258], 'containing': [2212, 2314, 2322, 3128, 3292, 3317, 3397, 3678], '10': [2213, 3565, 3706, 4239], '1,10': [2215], 'o-phenanthroline': [2216], '(Sigma)': [2217], '(31Lee': [2218], 'Dorwart': [2220], '184:': [2235, 3379, 3458, 3533, 3629], '2300-2304Crossref': [2236], '(78)': [2239], 'Restoration': [2242], 'achieved': [2248], 'sequential': [2250], '6.5),': [2265], 'respectively.': [2266, 2749, 3121], 'adjusted': [2270], 'between': [2272], '25': [2273], '35': [2275], 'aliquoted': [2278], '1.1-ml': [2280], 'portions': [2281], 'into': [2282, 2522, 2588, 2614, 2702, 2825, 2883, 2894, 2981, 3020, 3078, 3338, 3394, 3550, 3668, 3904], '8000': [2283], 'molecular': [2284], 'weight': [2285], 'cut-off': [2286], 'bags': [2288, 2313], '(SPECTRA/POR,': [2289], 'Spectrum': [2290], 'Medical': [2291], 'Industries,': [2292], 'Inc,': [2293], 'Los': [2294], 'Angeles,': [2295], 'CA).': [2296, 4065], 'Thereafter,': [2297], 'manipulations': [2300, 3246], 'employed': [2301], 'acid-washed': [2302, 3250], 'plastic': [2303], 'containers': [2304], 'buffers': [2306], 'treated': [2307, 2550, 4078], 'Chelex-100': [2309], '(Bio-Rad).': [2310], 'apo-protein': [2315], 'transferred': [2317], '50-ml': [2319], 'conical': [2320], 'tubes': [2321], '45': [2323, 4245], 'ml': [2324, 3606], '6.5)': [2331], '(0–100': [2337], 'μm)': [2338], 'dialyzed': [2340], 'overnight.': [2341, 3277], 'Protein': [2342], 'dialysate': [2344], 'samples': [2345], 'analyzed': [2347], 'inductively': [2351], 'coupled': [2352], 'plasma': [2353], 'emission': [2354], 'spectroscopy': [2355, 2364], 'graphite': [2360], 'furnace': [2361], 'atomic': [2362], 'absorption': [2363], 'Mayo': [2367], 'Clinic': [2368], '(Rochester,': [2369], 'MN).': [2370], 'amino': [2377], 'analysis.': [2379, 2948], 'Metal-toproteins': [2380], 'ratios': [2381], '(θ)': [2382], 'plotted': [2384], '(Me2+)': [2389], '(μm)': [2391], 'fitted': [2393], 'sigmoidal': [2395, 2448], 'curves': [2396, 2449], 'equation': [2399], 'θ': [2400], '=': [2401, 2411], '((N[Me2+])/(Kd': [2402], '+': [2403, 2405], '[Me2+]))': [2404], '0.3,': [2406], 'N': [2408], '(Me2+-binding': [2409], 'sites)': [2410], 'correction': [2438, 2458], 'factor': [2439], '0.3': [2441], 'included': [2443], 'better': [2445], 'fit': [2446], 'data;': [2452], 'Kd': [2453], 'values': [2454, 3766], 'obtained': [2455], 'without': [2456, 3298, 4186], 'did': [2459], 'differ': [2461], 'those': [2464], 'work.': [2468, 2664], 'znu': [2471, 2482, 2491, 2560, 2650, 2997], 'mntH': [2473, 2668, 2678, 2729, 2805], 'Mutants': [2474], 'Complementation': [2476, 3184, 3193, 3344], 'Plasmids—To': [2477], 'knockout': [2483, 2669], 'pSucZnu3.5,': [2485, 2610], 'recovered': [2487], 'wild': [2489], 'type': [2490], 'locus': [2492, 2561], 'Sau3AI': [2495], 'library': [2497], '(32Pendrak': [2498], 'Met.': [2503], '1991;': [2504, 2833, 3093], '41-47Crossref': [2506], '(34)': [2509], 'znuA': [2514], 'probe': [2515], 'subcloned': [2517], '9.4-kb': [2519], 'BglII/Asp718': [2520], 'fragment': [2521, 2583, 2822, 3033], 'pWSK129,': [2523, 3022], 'pZnu2,': [2525], 'complementation': [2531], 'intermediate': [2537], 'construction': [2540], 'pSucZnu3.5.': [2542], 'pZnu2': [2543], 'digested': [2545, 2692, 3071, 3897], 'EcoRI': [2547, 2876, 2880, 2896, 3063, 3073, 3179, 3891], 'ApaI,': [2549], 'Klenow,': [2552], 'self-ligated,': [2554], 'pZnu3,': [2556], 'harbors': [2558], '1265-bp': [2564], 'deletion': [2565], 'covering': [2566], 'approximately': [2567], 'half': [2568], 'znuB,': [2570], 'znuC,': [2573], 'bi-directional': [2576], 'znuCB/znuA': [2577], 'promoter.': [2578], '7.5-kb': [2580], 'Δznu': [2581], 'XbaI': [2582], 'pZnu3': [2585], 'ligated': [2587], 'suicide': [2590, 2704], 'vector': [2591, 2705, 3907], 'pSuc1': [2592], '(33Fetherston': [2593], 'Bertolino': [2595], 'V.J.': [2596], '32:': [2602, 2625, 2774, 3238], '289-299Crossref': [2603], '(128)': [2606], 'electroporated': [2613], 'KIM6+': [2617], '(34Bearden': [2618, 2767, 3231], '403-414Crossref': [2626, 2775, 3239], '(212)': [2629, 2778, 3242], 'Clones': [2632, 2781], 'arising': [2633, 2782], 'double': [2635, 2784], 'cross-over': [2636, 2785], 'events': [2637, 2786], 'selected': [2639, 2788], 'successively': [2641, 2790], 'screening': [2642, 2791], 'ampicillin': [2644, 3136, 3681], 'sucrose': [2646, 2795], 'resistance.': [2647, 2796], 'A': [2648, 3163, 3851], 'Δ': [2649], 'isolate': [2651], '(KIM6–2077+)': [2652], 'whose': [2653], 'genotype': [2654], 'Southern': [2658], 'blot': [2659], 'plasmid,': [2670], '3′': [2674, 2747, 2879, 3009, 3014, 3066, 3182, 3890], 'regions': [2675], 'PCR-amplified': [2681, 2858, 3050], 'primer': [2687, 2817, 2950, 3885, 4161], 'pairs': [2688, 2951, 3886], 'YpMntH5′-SalI/YpMntH5′-SmaIR': [2689], 'YpMntH3′-SmaIF/YpMntH3′-SpeI,': [2691], 'SalI/SmaI': [2694], 'SmaI/SpeI,': [2696], 'respectively,': [2697, 2978], 'cloned': [2699, 2824, 2882, 3019, 3077, 3152, 3903], 'tandem': [2701], 'SalI/SpeI-digested': [2703], 'pKNG101': [2706], '(35Sarker': [2707], 'Cornelis': [2709], 'G.R.': [2710], '23:': [2714], '410-411Crossref': [2715], '(40)': [2718], 'creating': [2721, 3116], 'pΔMntH.': [2722], 'resulting': [2724, 2821, 2992, 3911], 'insert': [2725, 2891, 3153], 'consists': [2726], 'gene,': [2730], 'lacking': [2731], '600': [2732], 'bp': [2733, 2742, 3037], 'coding': [2735], 'sequence,': [2736], 'flanked': [2737], '300': [2739], '80': [2741], 'ends,': [2748], 'Plasmid': [2750, 2797], 'pΔMntH': [2751], 'transform': [2755], 'KIM6': [2758, 2814], 'isogenic': [2761], 'yfe': [2762], 'mutant': [2763], 'KIM6–2031.1': [2766], 'streptomycin': [2793], 'pMntH-Op': [2798], 'PCR': [2802, 2954, 4173, 4179, 4214], 'amplifying': [2803], 'native': [2809], 'promoter': [2810, 2932], 'pair': [2818], 'YpMntH5′-SalI/YpMntH3′-XbaI;': [2819], 'pWSK129': [2826, 3084], '(36Wang': [2827, 3087], 'R.F.': [2828, 3088], 'Kushner': [2829, 3089], 'S.R.': [2830, 3090], '100:': [2834, 3094], '195-199Crossref': [2835, 3095], '(991)': [2838, 3098], 'opposite': [2843], 'orientation': [2844, 2928], 'vector-encoded': [2847, 3113], 'lacZ': [2848, 3114], 'gene.': [2849], 'Construction': [2850], 'Permease': [2852], 'Plasmids—The': [2853], 'promoter-less': [2854], 'troABCD': [2855], 'chromosomal': [2862], 'proofreading': [2866], 'polymerase': [2867, 3057, 4193, 4202], 'Ex-Taq': [2868, 3056], '(Panvera,': [2869], 'Madison,': [2870, 4084], 'WI),': [2871], 'NoPro': [2873, 3176], 'TroD': [2878], 'TOPO': [2884], 'pCR2.1': [2885], '(Invitrogen).': [2886, 3909], 'Following': [2887, 3719, 4205], 'confirmation,': [2889], 'moved': [2893], 'pBAD18': [2899, 3543], '(37Guzman': [2900], 'Belin': [2902], 'Carson': [2904], 'Beckwith': [2906], '1995;': [2910], '177:': [2911], '4121-4130Crossref': [2912], '(3876)': [2915], 'harboring': [2921], 'same': [2927], 'pBAD': [2931], '(pTroA-D)': [2933], 'identified.': [2935], 'pTroA(H199E)-D': [2936], 'pTroA-D': [2940], 'above': [2943], 'amplify': [2955], 'mntH,': [2956], 'yfeABCD,': [2957], 'scaCBA,': [2958], 'adcCBA': [2960], 'K-12,': [2964], 'KIM6,': [2967], 'gordonii': [2969], 'DL1,': [2970], 'Streptococcus': [2972], 'pneumoniae': [2973], 'TIGR': [2974], '4': [2975], 'DNAs,': [2977], 'cloning': [2980], 'pBAD18,': [2982], 'plasmids.': [2993], 'divergently': [2995], 'transcribed': [2996], 'K12': [3002], 'amplified': [3004, 3883], 'Ec': [3007, 3012], 'ZnuA': [3008], 'KpnI': [3010], 'ZnuB': [3013], 'XbaI,': [3015], 'digested,': [3016], 'directly': [3018], 'KpnI/XbaI-digested': [3021], 'pEcZnu.': [3024], 'clone': [3026], 'complete': [3028], 'extending': [3034], '106': [3036], 'upstream': [3038], 'troA': [3041], 'start': [3042], 'gpm': [3046, 3878], 'stop': [3047], '(Panvera)': [3058], 'TroPro': [3061], 'SacII.': [3067, 3183], 'amplicon': [3069], 'SacII': [3075], 'EcoRI/SacII': [3080], 'sites': [3081], 'pWSK29': [3086], 'such': [3101], 'oriented': [3107], 'oppositely': [3108], 'pPro-Gpm': [3117, 3119], 'Amp,': [3120], 'Transformants': [3122], 'plated': [3124, 3672], 'agar': [3127, 3263], '50': [3129, 3582], 'μg/ml': [3130, 3135, 3142], 'kanamycin': [3131], '(pPro-Gpm)': [3132], '100': [3134], '(pPro-GpmAmp),': [3137], 'isopropyl-β-d-thiogalactopyranoside,': [3140], '20': [3141], '5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside': [3143], '(X-gal)': [3144], '30': [3149, 3303, 3333, 3592, 4212, 4228], '°C.': [3150], 'sequenced': [3155], 'listed': [3158, 4154], 'materials.': [3162], 'variant,': [3164], 'pNoPro-Gpm,': [3165], 'lacks': [3167, 3512], 'similarly': [3172], 'Transporter': [3191], 'Mutants—': [3192], 'conducted': [3200, 3248, 3354, 3443], 'broth': [3203, 3575], 'chelator': [3225, 3314, 3411], 'gradient': [3226, 3315], 'plates': [3227, 3316, 3677, 3702], 'Scholar);': [3244], 'glassware.': [3251], 'broth,': [3255, 3558], 'cultivated': [3259], 'tryptose': [3261], 'blood': [3262], 'slants': [3264], 'A620': [3272, 3286], 'following': [3279, 4219], 'morning': [3280], 'they': [3281], 'diluted': [3283, 3393, 3549, 3666], 'fresh': [3290, 3395, 3552, 3669], 'required': [3294], 'antibiotics': [3295], 'arabinose.': [3300], 'Growth': [3301], '°C': [3304, 3421, 4210, 4222, 4232, 4237, 4243, 4255], 'shaking': [3307], 'water': [3308], 'bath': [3309], 'spectrophotometrically': [3311, 3428], 'monitored.': [3312], 'Arabinose-containing': [3313], '0–1': [3318], 'TPEN': [3320, 3412], '0–5': [3322], 'conalbumin': [3324], 'inoculated': [3326], 'incubated': [3328, 3418, 3990], '3': [3330, 3561, 4257], 'days': [3331], '°C,': [3334, 3724], 'after': [3335], 'metaldepleted': [3340], 'zones': [3341, 3726, 3748], 'measured.': [3343], 'uptake-deficient': [3349], 'broth.': [3357], 'Overnight': [3358, 3546], 'cultures': [3360, 3416, 3547, 3664], 'GR354': [3364], '(Δznu/ΔzupT)': [3365], '(38Grass': [3366, 3520], 'G.': [3367, 3521], 'Wong': [3368, 3522], 'Rosen': [3370, 3524], 'B.P.': [3371, 3525], 'Smith': [3372, 3526], 'Rensing': [3374, 3528], '864-866Crossref': [3380, 3534], '(133)': [3383, 3537], 'bearing': [3386], 'control': [3388, 4181], 'antibiotics,': [3399], '0': [3400, 3684], 'arabinose,': [3403], 'various': [3405], 'ZnCl2.': [3414], '37': [3420, 3723], 'vigorous': [3423], 'aeration,': [3424], 'monitored': [3429], '12': [3431], 'h.': [3432], '65Zn': [3433, 3659], '54Mn': [3435], 'Transport': [3436], 'Assays—With': [3437], 'minor': [3438], 'variation,': [3439], 'transport': [3440], 'assays': [3441], '(39Kehres': [3447, 3618], 'D.G.': [3448, 3466, 3489, 3619], 'Janakiraman': [3449, 3620], 'Slauch': [3451, 3622], 'Maguire': [3453, 3471, 3494, 3624], 'M.E.': [3454, 3472, 3495, 3625], '3159-3166Crossref': [3459, 3630], '40Kehres': [3465], 'Zaharik': [3467, 3490], 'Finlay': [3469, 3492], 'B.B.': [3470, 3493], '36:': [3476, 3499], '1085-1100Crossref': [3477, 3500], '(228)': [3480, 3503], 'MM2115': [3486], '(ΔmntH)': [3487], '(40Kehres': [3488], 'GR362,': [3510], 'known': [3514], 'efflux': [3518], 'transporters': [3519], 'transformed': [3541], 'pTroA-D.': [3545], 'Chelex': [3553], '100-treated': [3554], 'tryptone,': [3555], '0.25%': [3556], 'NaCl': [3557], 'h,': [3562], 'induced': [3563, 3924], 'arabinose': [3567], '15–30': [3569], 'min,': [3570, 3596], 'added': [3572], 'isotope-containing': [3574], '(100': [3576], 'nm65Zn2+': [3577], '(Perkin-Elmer,': [3578], 'Boston,': [3579], 'MA)': [3580], 'nm': [3583], '54Mn2+': [3584], '(Perkin-Elmer)).': [3585], 'Culture': [3586], 'samples,': [3587], 'taken': [3588], 'intervals': [3590], 's': [3593, 4246], '5': [3595, 3605, 4234], 'collected': [3598], '0.45-μm': [3600], 'filters,': [3601], 'rinsed': [3602], 'twice': [3603], 'ice-cold': [3608], 'medium,': [3609], 'processed': [3611], 'scintillation': [3613], 'counting': [3614], 'Channels': [3636], '401–945': [3637], 'detect': [3641, 3655], 'photons': [3642, 3656], 'derived': [3643, 3657], 'γ': [3645], 'radiation': [3646], '54Mn,': [3648], 'channels': [3651], 'radiation.': [3660], 'Metal': [3661], 'Sensitivity': [3662], 'Assays—Overnight': [3663], '1:100': [3667], '1%': [3675], 'agarose': [3676], 'mg/ml': [3680], 'l-arabinose.': [3687], 'Sterile': [3688], '6-mm': [3689], 'paper': [3690], 'disks': [3691], 'Dickinson)': [3693], 'placed': [3695], 'center': [3698], 'air-dried': [3701], 'impregnated': [3704], 'μl': [3707], '0.5': [3709], 'ZnCl2,': [3711], 'MnCl2,': [3714], 'FeSO4.': [3718], 'incubation': [3721], 'inhibition': [3728], 'measured': [3730, 4053], 'blinded': [3732], 'fashion.': [3733], 'measurements': [3735], 'three': [3737, 4163], 'seven': [3739], 'independent': [3740], 'calculate': [3745], 'mean': [3747, 3754], 'inhibition.': [3750], 'differences': [3752], 'zone': [3755], 'sizes': [3756], 'compared': [3758], 'two-tailed': [3761], "Student's": [3762], 't': [3763], 'test;': [3764], 'p': [3765], '≤': [3767], '0.01': [3768], 'considered': [3770], 'significant.': [3771], 'Although': [3772], 'Fe2+': [3773], '(FeSO4)': [3774], 'lability': [3781], 'ion': [3784], 'aerobic': [3786], 'conditions': [3787], 'allowed': [3789], 'oxidation': [3791], 'Fe3+': [3794], 'ion.': [3795], 'Immunologic': [3796], 'Reagents': [3797], 'Immunoblot': [3799], 'Analysis—Polyclonal': [3800], 'rat': [3801], 'antiserum': [3802, 3999], 'directed': [3803, 3855, 3870], '(14Akins': [3810], 'Antiserum': [3830], 'adsorbed': [3832], '(pWSK129)': [3836], 'lysates': [3837, 3928, 3976], 'electrophoresed': [3841], '12.5%': [3844, 3980], 'SDS-polyacrylamide': [3845, 3981], 'gel': [3846], 'electrotransferred': [3848, 3984], 'nitrocellulose.': [3850, 3986], 'mouse': [3852], 'monoclonal': [3853, 4002], 'antibody': [3854, 4003], 'σ70': [3859], 'purchased': [3861], 'NeoClone': [3863], 'Biotechnology': [3864, 4019], '(Madison,': [3865], 'WI).': [3866], 'produce': [3868], 'antisera': [3869], 'TroR,': [3874], 'troR': [3880], 'NcoI/Gpm': [3889], 'NcoI/TroR': [3895], 'EcoRI,': [3896, 3901], 'NcoI': [3899], 'pProExHTa': [3908], '(pHTa:Gpm': [3913], 'pHTa:TroR)': [3915], 'encode': [3916], 'cell': [3927], 'chromatography': [3932], 'immunize': [3936], '4-week-old': [3937], 'male': [3938], 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'Affinity—TroA': [4271], '0.96': [4277], 'atoms/protein': [4279], 'molecule': [4280], 'However,': [4305], 'result': [4307], 'reflect': [4309], 'relatively': [4311], 'level': [4313], '(41Outten': [4319], 'C.E.': [4320], "O'Halloran": [4321], 'T.V.': [4322], '292:': [4325], '2488-2492Crossref': [4326], '(1122)': [4329]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2031989139', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 2}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 3}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 4}, {'year': 2012, 'cited_by_count': 2}], 'updated_date': '2025-01-05T22:39:35.895151', 'created_date': '2016-06-24'}