Characterization of a Thermophilic P-type Ag+/Cu+-ATPase from the ExtremophileArchaeoglobus fulgidus

Name: Work Video:
Duration: 3 min 30 s
A. Mandal; Win D. Cheung; José Argüello
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358, 376, 382, 430, 436, 497, 503, 3393, 3529, 3538, 3544, 3557, 3571, 4429], '103': [54, 331, 4504], 'kJ/mol)': [55, 332], 'inactive': [57, 334, 1960, 4491], '37': [59, 336, 2498, 3176, 3287, 4493], '°C.': [60, 337, 2536, 2645, 2697, 2869, 3062, 3288, 4494], 'It': [61, 338, 4173], 'activated': [63, 340], 'Ag+(ATPase': [65, 342], 'V': [66, 79, 343, 356, 3349, 3370, 3391, 3469, 3527, 3536, 3555, 3564], 'max': [67, 80, 344, 357, 3320, 3342, 3392, 3528, 3556], '14.82': [69, 346], 'μmol/mg/h)': [70, 347], 'to': [72, 142, 204, 235, 270, 349, 419, 481, 512, 547, 889, 896, 1398, 1559, 1563, 1600, 1656, 1660, 1945, 2591, 2732, 2786, 3261, 3317, 3339, 3389, 3722, 3784, 3846, 3894, 4044, 4170, 4184, 4328, 4391, 4421], 'lesser': [74, 351], 'extent': [75, 352], 'Cu+': [77, 85, 102, 113, 354, 362, 379, 390, 1152, 1561], '(ATPase': [78, 94, 217, 355, 371, 494], '3.66': [82, 359], 'μmol/mg/h).': [83, 360], 'However,': [84, 162, 361, 439, 1590, 2079, 4298], 'interacts': [86, 209, 363, 486], 'with': [87, 90, 138, 210, 213, 364, 367, 415, 487, 490, 1055, 1662, 2238, 2492, 2505, 2513, 2523, 2573, 2673, 2726, 2743, 2752, 2776, 2806, 2818, 2844, 2877, 2919, 2936, 3069, 3111, 3178, 3204, 3293, 4009, 4023, 4032, 4270, 4444], 'enzyme': [89, 212, 236, 366, 489, 513, 1527, 2593, 3233, 3312, 4045, 4332, 4472], 'higher': [91, 368, 4426], 'apparent': [92, 139, 215, 369, 416, 492, 1935], 'affinity': [93, 370, 2465, 4069], 'stimulation,': [95, 372], 'Ag+': [96, 111, 373, 388], 'K': [97, 103, 151, 157, 218, 224, 374, 380, 428, 434, 495, 501, 3472, 3542, 3569], '12': [98, 104, 152, 158, 375, 381, 429, 435, 3358, 3543, 3570], '29.4': [100, 377], 'μm;': [101, 155, 378, 432], '2.1': [106, 383], 'μm).': [107, 161, 240, 384, 438, 517], 'This': [108, 241, 385, 518, 857, 1404, 1941, 2286, 2455, 4374], 'activation': [109, 386, 1967, 4502], 'or': [112, 389, 1462, 1644, 2985, 3157, 3255, 3283, 3307, 3920, 4324, 4383, 4417], 'dependent': [115, 392], 'on': [116, 393, 861, 955, 1391, 2220, 2259, 2801, 2897, 3240, 3776, 4054, 4250, 4267, 4350, 4442, 4463], 'presence': [118, 126, 171, 231, 395, 403, 448, 508, 1453, 1625, 3999, 4310], 'millimolar': [120, 397], 'amounts': [121, 398, 4161], 'cysteine.': [123, 400], 'ATP,': [128, 405, 2963, 3308], 'metals': [130, 407, 1069, 1658], 'drive': [131, 408], 'formation': [133, 410, 1259], 'an': [135, 271, 412, 548, 4499], 'acid-stable': [136, 413], 'phosphoenzyme': [137, 166, 414, 443], 'affinities': [140, 216, 417, 493], 'similar': [141, 203, 418, 480, 3845, 3893, 4041, 4386], 'those': [143, 420, 3847], 'observed': [144, 421, 2914], 'in': [145, 169, 249, 422, 446, 526, 703, 722, 879, 915, 949, 1045, 1063, 1088, 1149, 1156, 1464, 1469, 1631, 1838, 1914, 1924, 2013, 2281, 2307, 2500, 2598, 2688, 2706, 2864, 2875, 2999, 3067, 3094, 3104, 3116, 3212, 3219, 3246, 3444, 3452, 3819, 3838, 3993, 4002, 4102, 4166, 4308, 4395], 'ATPase': [147, 424, 2295, 2945, 3053, 3303, 3332, 4290, 4348, 4414, 4462], 'activity': [148, 425, 2946, 3054, 3304, 3333, 3374, 3509, 3577, 4028, 4291, 4301, 4349, 4441, 4447, 4485], 'determinations': [149, 426, 2872], '(Ag+,': [150, 175, 427, 452], '23.0': [154, 431], 'Cu+,': [156, 178, 433, 455, 564, 3306], '3.9': [160, 437], 'comparable': [163, 440], 'levels': [164, 441], 'are': [167, 444, 907, 1151, 1159, 1487, 1750, 1909, 1959, 3432, 3476, 3885], 'reached': [168, 445, 4483], 'both': [173, 450], 'cations': [174, 187, 451, 464], '1.40': [176, 453], 'nmol/mg;': [177, 454], '1.08': [179, 456], 'nmol/mg).': [180, 457], 'stimulation': [182, 459], 'phosphorylation': [184, 223, 461, 500, 698, 1476, 3110, 3242, 3313], 'suggests': [188, 465, 1889], 'that': [189, 466, 912, 1519, 1746, 1890, 2068, 3363, 4088, 4176, 4192, 4306, 4467, 4481], 'drives': [191, 468], 'outward': [193, 470], 'movement': [194, 471], 'metal.': [197, 474], 'presents': [199, 476], 'additional': [200, 477], 'characteristics': [202, 479], 'other': [205, 482, 2005, 4033, 4106, 4315], 'P-type': [206, 483, 554, 893, 944, 979, 2294, 3601, 4110], 'ATP': [208, 485, 578, 919, 3112, 3258, 4507], 'm': [219, 225, 496, 502], '0.25': [221, 498], 'mm;': [222, 499], '4.81': [227, 504], 'μm),': [228, 505], 'vanadate': [233, 510, 3503], 'leads': [234, 511], 'inactivation': [237, 514, 4424], '(IC50=': [238, 515], '24': [239, 516, 3545], 'first': [244, 521, 2289], 'Ag+/Cu+-ATPase': [245, 522], 'expressed': [246, 523], 'purified': [248, 525, 2301, 2976, 3148, 4065, 4109, 4284], 'form.': [252, 529], 'Thus,': [253, 530, 1550, 1906, 2215], 'it': [254, 531, 1515, 2064, 4419, 4477], 'provides': [255, 532], 'model': [257, 534], 'structure-functional': [259, 536], 'studies': [260, 537, 1743, 3981], 'transporters.': [263, 276, 540, 553], 'Moreover,': [264, 541, 1603, 3880], 'its': [265, 542, 3820, 4058, 4268], 'will': [267, 544, 3720], 'also': [268, 545, 728], 'contribute': [269, 546], 'understanding': [272, 549, 2242], 'thermophilic': [274, 551, 2007, 2069, 2226, 2236, 2293], 'ion': [275, 552, 880, 903, 924, 971, 1057, 1594, 1608, 1754, 1938, 2251, 2261], 'ATPases': [555, 894, 945, 980, 1062, 1147, 1253, 1396, 2142, 2255, 3602, 3724, 3760, 3792, 4111], 'variety': [558], 'ions': [560], '(H+,': [561], 'Na+,': [562], 'K+,': [563], 'Ca2+,': [565], 'Mg2+,': [566], 'Cd2+,': [567, 1073, 4322], 'Zn2+,': [568, 1072, 4321], 'etc.)': [569], 'across': [570], 'cell': [571], 'membranes': [572, 2026, 2703, 2933, 4004], 'using': [573, 2429, 2835, 3019, 3423, 3494, 3526, 3554, 4071], 'energy': [575, 921, 3379, 4500], 'provided': [576, 858], 'hydrolysis': [579, 4508], '(1Axelsen': [580, 984, 1030], 'K.B.': [581, 985, 1031], 'Palmgren': [582, 986, 1029, 1032], 'M.G.': [583, 987, 1033, 2121], 'J.': [584, 765, 786, 791, 813, 988, 1016, 1034, 1235, 1279, 1280, 1300, 1317, 1337, 1380, 1429, 1679, 1705, 1772, 1816, 1817, 2049, 2127, 2151, 2154, 2182, 2317, 2331, 2343, 2367, 2375, 2377, 2413, 2557, 3613, 3627, 3639, 3663, 3671, 3673, 3709, 3799, 3862, 3934, 4132, 4223], 'Mol.': [585, 989, 1017, 1035, 1196, 1856, 1976, 2105, 2201], 'Evol.': [586, 990, 1018, 1036], '1998;': [587, 991, 1037, 1542, 1582, 2036, 4210], '46:': [588, 992, 1038], '84-101Crossref': [589, 993, 1039], 'PubMed': [590, 618, 629, 694, 756, 776, 802, 824, 852, 994, 1007, 1022, 1040, 1108, 1120, 1137, 1178, 1201, 1221, 1241, 1291, 1311, 1324, 1348, 1369, 1385, 1440, 1503, 1545, 1585, 1690, 1716, 1737, 1783, 1796, 1828, 1860, 1880, 1980, 2000, 2039, 2059, 2111, 2133, 2165, 2187, 2206, 2418, 2568, 2886, 2906, 3048, 3086, 3714, 3804, 3871, 3915, 3945, 3973, 4137, 4150, 4213, 4233], 'Scopus': [591, 630, 757, 777, 803, 825, 853, 995, 1008, 1023, 1041, 1109, 1121, 1138, 1179, 1202, 1222, 1242, 1292, 1312, 1325, 1349, 1370, 1386, 1441, 1504, 1546, 1586, 1691, 1717, 1738, 1784, 1797, 1829, 1861, 1981, 2040, 2060, 2134, 2166, 2207, 2419, 2569, 2887, 2907, 3049, 3087, 3715, 3916, 3946, 3974, 4138, 4151, 4214, 4234], '(769)': [592, 996, 1042], 'Google': [593, 619, 632, 695, 759, 779, 805, 827, 855, 997, 1010, 1025, 1043, 1111, 1123, 1140, 1181, 1204, 1224, 1244, 1294, 1314, 1327, 1351, 1372, 1388, 1443, 1506, 1548, 1588, 1693, 1719, 1740, 1786, 1799, 1831, 1863, 1881, 1983, 2001, 2042, 2062, 2112, 2136, 2168, 2188, 2209, 2421, 2571, 2889, 2909, 3051, 3089, 3717, 3805, 3872, 3918, 3948, 3976, 4140, 4153, 4216, 4236], 'Scholar,': [594, 609, 620, 685, 760, 780, 806, 998, 1011, 1112, 1124, 1182, 1205, 1225, 1295, 1315, 1328, 1352, 1373, 1694, 1720, 1787, 1800, 1864, 1984, 2043, 2113, 2169, 4126, 4141, 4217], '2Glynn': [595], 'I.': [596, 672, 1188, 1802, 3957, 4113], 'Martonosi': [597, 673, 4114], 'A.': [598, 674, 1299, 1365, 1422, 2051, 2200, 2309, 2335, 2369, 3589, 3631, 3665, 3855, 3954, 4115, 4225, 4281], 'Enzymes': [599, 675, 4116], 'Biological': [601, 677, 4118], 'Membranes.': [602, 678, 4119], '3.': [603, 679, 4120], 'Plenum': [604, 680, 4121], 'Press,': [605, 681, 4122], 'New': [606, 682, 3416, 4123], 'York1985:': [607, 683, 4124], '35-114Google': [608, 684, 4125], '3Inesi': [610, 686], 'G.': [611, 687, 749, 1426, 2099, 2119, 2147, 2347, 2405, 3643, 3701], 'Annu.': [612, 688], 'Rev.': [613, 689, 1854, 1974, 2107], 'Physiol.': [614, 690], '1985;': [615, 691, 4147], '47:': [616, 692], '573-601Crossref': [617, 693], '4Lutsenko': [621, 999], 'S.': [622, 1000, 1114, 1186, 1231, 1233, 1364, 1670, 1698, 1771, 1812, 2045, 2196, 2349, 2361, 2391, 2401, 3041, 3645, 3657, 3687, 3697, 4219], 'Kaplan': [623, 1001, 1191, 1677], 'J.H.': [624, 1002, 1192, 1678], 'Biochemistry.': [625, 1003, 1320, 1792, 3044], '1995;': [626, 768, 1004, 1303], '34:': [627, 1005], '15607-15613Crossref': [628, 1006], '(420)': [631, 1009], 'Scholar).': [633, 696, 828, 856, 1026, 1141, 1245, 1389, 1444, 1507, 1549, 1589, 1741, 1832, 1882, 2002, 2890, 2910, 3052, 3090, 3418, 3718, 3806, 3977, 4237], 'study': [635, 1884, 2234], 'key': [637], 'members': [638, 735, 1272], 'family,': [641, 738], 'like': [642], 'Na,K-ATPase': [644, 740], 'sarcoplasmic': [647, 926], 'reticulum': [648, 927], '(SR)': [649], '1SRsarcoplasmic': [650], 'reticulumDDMdodecyl': [651], 'β-d-maltosideDTTdithiothreitolN-MBDN-terminal': [652], 'binding': [654, 724, 933, 1458, 1629, 3817, 3836], 'domainTMtransmembrane': [655], 'segmentMES4-morpholineethanesulfonic': [656], 'acidCHES2-(cyclohexylamino)ethanesulfonic': [657], 'acid': [658, 938, 940, 1898, 2770, 3205, 3270, 4068], 'Ca-ATPase,': [659], 'has': [660, 727, 973, 1254, 1407, 1516, 1666, 1760, 3732, 3808, 3827], 'allowed': [661, 974], 'description': [663, 2290], 'significant': [665, 884, 1842, 4175], 'aspects': [666], 'their': [668, 1080, 1465, 1632, 1846, 1904], 'catalytic': [669, 2244], 'cycle': [670], '(2Glynn': [671, 4112], 'aspartyl': [701], 'group': [702], 'consensus': [705, 1642], 'sequence': [706, 1459, 1655, 1899, 2475, 2481, 3777, 3818, 3837], 'DKTGT': [707, 3771], 'fundamental': [710], 'characteristic': [711, 1620], 'within': [712, 3789], 'mechanism.': [714], 'participation': [716, 871], 'specific': [718], 'transmembrane': [719, 935, 1466], 'segments': [720], '(TMs)': [721], 'cation': [723], 'been': [729, 947, 1256, 1268, 1408, 1517, 1667, 1761, 1947, 2096, 2212, 3733], 'established': [730, 1668], 'better': [733], 'characterized': [734], 'SR': [742, 834], 'Ca-ATPase': [743, 835], '(5Clarke': [744], 'D.M.': [745], 'Loo': [746], 'T.W.': [747], 'Inesi': [748], 'MacLennan': [750], 'D.H.': [751], 'Nature.': [752, 848, 2414, 2902, 3710], '1989;': [753], '339:': [754], '476-478Crossref': [755], '(518)': [758], '6Argüello': [761], 'J.M.': [762, 782, 810, 1211], 'Lingrel': [763, 787, 811], 'J.B.': [764, 788], 'Biol.': [766, 792, 814, 1281, 1301, 1338, 1430, 1680, 1706, 1733, 1773, 1818, 2106, 2155, 2558, 3863, 3935], 'Chem.': [767, 793, 815, 1282, 1302, 1339, 1431, 1681, 1707, 1774, 1819, 2156, 2559, 3864, 3936], '270:': [769, 1304], '22764-22771Abstract': [770], 'Full': [771, 773, 797, 799, 819, 821, 1134, 1175, 1286, 1288, 1306, 1308, 1343, 1345, 1435, 1437, 1500, 1685, 1687, 1711, 1713, 1778, 1780, 1823, 1825, 2160, 2162, 2563, 2565, 3868, 3940, 3942, 3968, 3970], 'Text': [772, 774, 798, 800, 820, 822, 1135, 1176, 1287, 1289, 1307, 1309, 1344, 1346, 1436, 1438, 1501, 1686, 1688, 1712, 1714, 1779, 1781, 1824, 1826, 2161, 2163, 2564, 2566, 3869, 3941, 3943, 3969, 3971], 'PDF': [775, 801, 823, 1136, 1177, 1290, 1310, 1347, 1439, 1502, 1689, 1715, 1782, 1827, 2164, 2567, 3870, 3944, 3972], '(71)': [778], '7Argüello': [781], 'Peluffo': [783], 'R.D.': [784], 'Feng': [785], 'Berlin': [789], 'J.R.': [790, 1213, 1700], '1996;': [794, 816, 1117, 1283, 1432, 1497, 2184], '271:': [795, 817, 1284, 1433], '24610-24616Abstract': [796], '(42)': [804], '8Kuntzweiler': [807], 'T.A.': [808], 'Argüello': [809], 'J.B': [812], '29682-29687Abstract': [818], '(92)': [826], 'Recently,': [829], 'structure': [831, 1893, 2222, 4249], '(2.6-Å': [836], 'resolution)': [837], 'was': [838, 2423, 2447, 2476, 2511, 2539, 2638, 2658, 2671, 2741, 2757, 2784, 2799, 2816, 2833, 2842, 2895, 2925, 3008, 3017, 3055, 3065, 3100, 3113, 3163, 3210, 3225, 3259, 3382, 3421, 3492, 3510, 3578, 4021, 4029, 4063, 4182, 4257, 4302, 4356, 4478, 4490], 'reported': [839, 3465, 3480], '(9Toyoshima': [840], 'C.': [841, 1227, 1332, 1354, 1493, 2363, 2397, 2399, 2407, 2411, 2552, 3659, 3693, 3695, 3703, 3707, 3794], 'Nakasako': [842], 'M.': [843, 1277, 1297, 1491, 1538, 1578, 1696, 1806, 1810, 2101, 2177, 2194, 2327, 2357, 2383, 3623, 3653, 3679, 3796, 3861, 3907, 3961, 4143], 'Nomura': [844], 'H.': [845, 847, 1415, 1532, 1572, 1808, 2181, 2313, 2409, 3609, 3705, 3857], 'Ogawa': [846], '2000;': [849, 1105, 1340, 1734, 1877, 1997, 2056, 2560, 3965, 4230], '405:': [850], '647-655Crossref': [851], '(1636)': [854], 'important': [859, 886], 'information': [860, 2217, 2258], 'overall': [863], 'disposition': [864], 'TMs': [867, 874, 1401, 3763], 'confirmed': [869, 1410, 2477], 'three': [873, 3440], '(H4,': [875], 'H5,': [876], 'H6)': [878], 'binding.': [881], 'Despite': [882], 'advances,': [885], 'questions': [887], 'pertaining': [888], 'family': [891, 1275], 'remain': [895], 'be': [897, 1601, 1616, 2066, 4171], 'answered.': [898], 'For': [899, 1142, 2698, 3446], 'instance,': [900, 1143], 'how': [901], 'selectivity': [904, 2252], 'achieved?': [905], 'What': [906], 'particular': [909, 1056, 1919, 2014, 2021, 2074], 'molecular': [910], 'events': [911], 'take': [913], 'place': [914], 'protein': [917, 2699, 2815, 2841, 2871, 2924, 3992, 4056, 4062, 4096], 'during': [918, 4057], 'hydrolysis,': [920], 'transduction,': [922], 'transport?': [925], 'dodecyl': [928], 'β-d-maltoside': [929], 'dithiothreitol': [930, 2860], 'N-terminal': [931], 'domain': [934], 'segment': [936], '4-morpholineethanesulfonic': [937], '2-(cyclohexylamino)ethanesulfonic': [939], 'More': [941], 'than': [942, 3102, 3227], '250': [943], 'have': [946, 1267, 1399, 1744, 1840, 1946, 1965, 2015, 2073, 2095, 2211, 2231, 3753], 'identified': [948], 'all': [950, 3105], 'life': [951], 'kingdoms': [952], '(data': [953], 'available': [954, 2219], 'World': [957], 'Wide': [958], 'Web': [959], 'biobase.': [961], 'dk/∼axe/Patbase.html).': [962], 'Comparison': [963], 'primary': [965], 'sequences,': [966, 1514], 'proposed': [967], 'topological': [968, 3756], 'arrangements,': [969], 'specificity': [972, 1595], 'organization': [976], 'into': [981, 2449], 'various': [982], 'subfamilies': [983], '10Fagan': [1012], 'M.J.': [1013, 1015, 1674], 'Saier': [1014], '1994;': [1019, 1131, 1172, 1198], '38:': [1020, 1322], '57-99Crossref': [1021], '(149)': [1024], 'Axelsen': [1027], 'Scholar),': [1044, 2063, 2137, 3949, 4154], 'detailed': [1047, 1248, 4239], 'analysis': [1048, 3420, 3486, 4240], 'phylogenetic': [1050], 'trees,': [1051], 'described': [1052, 3250, 3299], 'five': [1053, 3202], 'groups': [1054], 'specificities': [1058], '(PI–PV).': [1059], 'Among': [1060], 'these,': [1061], 'PIB': [1065], 'subfamily': [1066], 'transition': [1068, 3098, 3223], '(Cu+,': [1070], 'Ag+,': [1071], 'Pb2+,': [1074], 'Co2+).': [1076], 'light': [1078], 'substrates,': [1081], 'enzymes': [1083, 1486, 1908, 1958], 'appear': [1084, 1397, 3781, 3844], 'as': [1085, 2435, 2997, 3249, 3298, 3309, 3311, 3516, 3725, 3736, 4415], 'central': [1086], 'elements': [1087], 'processes': [1090], 'biotolerance': [1093], 'bioaccumulation': [1095], '(11Williams': [1096], 'L.E.': [1097], 'Pittman': [1098], 'J.K.': [1099], 'Hall': [1100], 'J.L.': [1101, 2030, 4204], 'Biochim.': [1102, 4144], 'Biophys.': [1103, 4145], 'Acta.': [1104, 4146], '1465:': [1106], '104-126Crossref': [1107], '(497)': [1110], '12Silver': [1113], 'Gene': [1115], '(Amst.).': [1116], '179:': [1118], '9-19Crossref': [1119], '(510)': [1122], '13Bull': [1125], 'P.C.': [1126, 1167, 1207, 1874, 1994], 'Cox': [1127, 1168, 1214, 1701], 'D.W.': [1128, 1169, 1215, 1702], 'Trends': [1129, 1170, 1494, 3962], 'Genet.': [1130, 1171, 1197, 1217, 1237], '10:': [1132, 1173], '246-252Abstract': [1133, 1174], '(282)': [1139, 1180], 'present': [1148, 2268, 2280], 'humans': [1150], 'transporters,': [1153], 'mutations': [1155], 'proteins': [1158, 1450, 1888, 2009, 2071, 4340], 'responsible': [1160], "Menkes'": [1162], "Wilson's": [1164], 'disease': [1165], '(13Bull': [1166], '14Petrukhin': [1183], 'K.': [1184, 1418, 1672, 2171, 2321, 2323, 2355, 2395, 3617, 3619, 3651, 3691], 'Lutsenko': [1185, 1770], 'Chernov': [1187], 'Ross': [1189], 'B.M.': [1190], 'Gilliam': [1193, 1675], 'T.C.': [1194], 'Hum.': [1195], '3:': [1199, 1239], '1647-1656Crossref': [1200], '(312)': [1203], '15Bull': [1206], 'Thomas': [1208], 'G.R.': [1209], 'Rommens': [1210], 'Forbes': [1212, 1699], 'Nat.': [1216, 1236, 1731], '1993;': [1218, 1238, 3865], '5:': [1219, 1878, 1998, 2057, 3046, 4231], '327-337Crossref': [1220], '(1755)': [1223], '16Vulpe': [1226], 'Levinson': [1228], 'B.': [1229, 1336, 1356, 1704, 1769, 1789, 2117, 2353, 2359, 2389, 2556, 3649, 3655, 3685, 4128], 'Whitney': [1230], 'Packman': [1232], 'Gitschier': [1234], '7-13Crossref': [1240], '(1238)': [1243], 'Although': [1246, 1917, 4403], 'enzymatic': [1249], 'not': [1255, 1751, 3450, 4399, 4479], 'performed,': [1257], 'phosphorylated': [1262, 3248], 'intermediate': [1263], 'properties': [1266], 'explored': [1269], 'some': [1271, 2081], '(17Solioz': [1276], 'Camakaris': [1278, 1815], '24465-24470Abstract': [1285], '(66)': [1293, 2061, 4235], '18Solioz': [1296], 'Odermatt': [1298], '9217-9221Abstract': [1305], '(238)': [1313], '19Okkeri': [1316], 'Haltia': [1318], 'T.': [1319, 1420, 1530, 1534, 1570, 1574, 2381, 2385, 2403, 3677, 3681, 3699, 3905], '1999;': [1321, 1820, 2108, 2157, 3801, 3912], '14109-14116Crossref': [1323], '(38)': [1326], '20Sharma': [1329], 'R.': [1330, 1767, 1791, 1850, 1866, 1870, 1970, 1986, 1990, 2149, 2175, 2315, 2325, 2341, 2373, 2550, 3611, 3621, 3637, 3669, 3859], 'Rensing': [1331, 2551], 'Rosen': [1333, 1357, 2553, 3797], 'B.P.': [1334, 1358, 2554, 3798], 'Mitra': [1335, 1355, 2555], '275:': [1341, 2561], '3873-3878Abstract': [1342, 2562], '(191)': [1350, 2570], '21Rensing': [1353], 'Proc.': [1359], 'Natl.': [1360], 'Acad.': [1361], 'Sci.': [1362, 1496, 3964], 'U.': [1363], '1997;': [1366, 1682, 1708, 2415, 3711, 3937], '94:': [1367], '14326-14331Crossref': [1368], '(355)': [1371], '22Tsai': [1374], 'K.J.': [1375], 'Yoon': [1376], 'K.P.': [1377, 1428], 'Lynn': [1378], 'A.R.': [1379], 'Bacteriol.': [1381, 2183, 3800], '1992;': [1382, 2130], '174:': [1383], '116-121Crossref': [1384], '(89)': [1387], 'Based': [1390, 3775], 'hydrophobicity': [1392], 'analysis,': [1393, 3779], 'most': [1394, 1955], 'eight': [1400, 3762], '(Fig.': [1402, 1478, 3773, 4011, 4074, 4097, 4333, 4451], '1).': [1403, 1479, 3774], 'membrane': [1405, 2008, 2070, 2739, 4197, 4248], 'topology': [1406], 'experimentally': [1409], 'only': [1411, 2138, 3599, 4303], 'from': [1414, 1474, 1834, 2093, 2143, 3922, 4341], 'pylori': [1416], '(23Melchers': [1417], 'Weitzenegger': [1419], 'Buhmann': [1421], 'Steinhilber': [1423], 'W.': [1424, 1852, 1972, 2053, 2153, 2179, 2198, 3037, 4227], 'Sachs': [1425], 'Schofer': [1427], '446-457Abstract': [1434], '(96)': [1442], 'A': [1445, 2600, 2675, 2690, 4238], 'unique': [1446], 'feature': [1447], 'putative': [1456, 3890, 4271, 4337], '(CPC,': [1460], 'CPH,': [1461], 'SPC)': [1463], 'region,': [1467], 'specifically': [1468], 'TM': [1471], 'immediately': [1472], 'upstream': [1473], 'site': [1477], 'Because': [1480], 'signature': [1483], 'sequence,': [1484], 'termed': [1488], 'CPx-ATPases': [1489, 1622, 2237, 3984, 4275], '(24Solioz': [1490], 'Vulpe': [1492], 'Biochem.': [1495, 1541, 1581, 1855, 1975, 2882, 3082, 3963, 4133], '21:': [1498], '237-241Abstract': [1499], '(422)': [1505], 'Concerning': [1508], 'role': [1511, 1759], 'shown': [1518, 1745], 'second': [1521], 'Cys': [1522], '(CPC)': [1523], 'required': [1525, 1752, 4156], 'function': [1528, 2224], '(25Yoshimizu': [1529, 1569], 'Omote': [1531, 1571], 'Wakabayashi': [1533, 1573], 'Sambongi': [1535, 1575], 'Y.': [1536, 1576], 'Futai': [1537, 1577], 'Biosci.': [1539, 1579, 1876, 1996, 2055, 4229], 'Biotechnol.': [1540, 1580], '62:': [1543, 1583], '1258-1260Crossref': [1544, 1584], '(32)': [1547, 1587], 'mutated': [1552], '(CPC': [1553], '→': [1554], 'CPA)': [1555], 'gene': [1556, 1567, 2474], 'unable': [1558, 4327], 'confer': [1560], 'resistance': [1562], 'Δccc2': [1564], 'yeast': [1565, 3923], '(Cu-ATPase': [1566], 'disrupted)': [1568], 'relationship': [1592], 'between': [1593], 'CPx': [1597], 'sequences': [1598], 'remains': [1599], 'established.': [1602], 'present,': [1606], 'transported': [1609], 'direction': [1612], 'cannot': [1615], 'predicted.': [1617], 'Another': [1618], 'noticeable': [1619], '1–6': [1627], 'domains': [1630, 1651, 1765], 'N': [1633], 'terminus': [1634], '(N-MBD).': [1635], 'These': [1636, 3744, 3842], 'cytoplasmic': [1637, 3767], 'N-MBDs': [1638, 1749], 'include': [1639], 'either': [1640, 2979, 3151], 'CXXC': [1643, 1654, 1748], 'His-rich': [1646, 3829], 'sequence.': [1647], 'capacity': [1649], 'containing': [1652, 3119, 3769], 'bind': [1657], 'interact': [1661], 'soluble': [1663, 1887], 'chaperones': [1665], '(26Lutsenko': [1669], 'Petrukhin': [1671], 'Cooper': [1673], 'C.T.': [1676, 3909], '272:': [1683, 1709, 3938], '18939-18944Abstract': [1684], '(215)': [1692], '27DiDonato': [1695], 'Narindrasorasak': [1697], 'Sarkar': [1703], '33279-33282Abstract': [1710], '(140)': [1718], '28Wernimont': [1721], 'A.K.': [1722], 'Huffman': [1723], 'D.L.': [1724], 'Lamb': [1725], 'A.L.': [1726], "O'Halloran": [1727], 'T.V.': [1728], 'Rosenzweig': [1729], 'A.C.': [1730], 'Struct.': [1732], '7:': [1735], '766-771Crossref': [1736], '(362)': [1739], 'Recent': [1742], 'transport,': [1755], 'regulatory': [1758], 'suggested': [1762], '(29Tsivkovskii': [1766], 'MacArthurs': [1768], '2001;': [1775, 1793, 1857, 1977, 2203], '276:': [1776], '2234-2242Abstract': [1777], '(136)': [1785], '30Mitra': [1788], 'Sharma': [1790, 2546], '40:': [1794], '7694-7699Crossref': [1795], '(95)': [1798], '31Voskoboinik': [1801], 'Strausak': [1803], 'D.': [1804, 2333, 2337, 3629, 3633, 3959], 'Greenough': [1805], 'Brooks': [1807], 'Petris': [1809], 'Smith': [1811, 2408, 3704], 'Mercer': [1813], 'J.F.': [1814], '274:': [1821, 2158], '22008-22012Abstract': [1822], '(104)': [1830], 'Proteins': [1833, 2911], 'hyperthermophile': [1835, 2283, 4199], 'organisms,': [1836, 4343], 'archaea': [1837, 2025, 2094, 4200], 'particular,': [1839], 'received': [1841, 2016], 'attention': [1843], 'because': [1844], 'extraordinary': [1847], 'stability': [1848, 1943], '(32Sterner': [1849, 1969], 'Liebl': [1851, 1971], 'Crit.': [1853, 1973], '36:': [1858, 1978], '39-106Crossref': [1859, 1979], '(333)': [1862, 1982], '33Scandurra': [1865, 1985], 'Consalvi': [1867, 1987], 'V.': [1868, 1988, 2103], 'Chiaraluce': [1869, 1989], 'Politi': [1871, 1991], 'L.': [1872, 1992, 2173, 2365, 2371, 2379, 3661, 3667, 3675], 'Engel': [1873, 1993], 'Front.': [1875, 1995, 2054, 4228], 'D787-D795Crossref': [1879, 1999], 'thermotolerant,': [1886], 'intrinsic': [1892], 'determined': [1894, 3066], 'amino': [1897, 3750], 'determinant': [1902], 'stability.': [1905], 'thermostable': [1907, 2260], 'obtained': [1910], 'after': [1911, 3172, 3264, 4005], 'expression': [1913, 2510, 2892, 4008], 'mesophilic': [1915], 'organisms.': [1916], 'no': [1918, 2586, 4051], 'stabilizing': [1920, 4052], 'mechanism': [1921], 'obvious': [1923], 'proteins,': [1926], 'general': [1928], 'increment': [1929], 'small': [1931, 3745], 'strengthening': [1932], 'forces': [1933], '(hydrogen': [1936], 'bonds,': [1937], 'pairs,': [1939], 'etc.).': [1940], 'structural': [1942, 2077, 2246], 'appears': [1944, 4376], 'achieved': [1948], 'expense': [1951], 'flexibility,': [1953], 'since': [1954, 4381], 'under': [1961, 3518, 4025, 4468, 4511], '40': [1962], '°C': [1963, 2499, 2751, 3016, 3023, 3177, 4296, 4488], 'energies': [1968], 'On': [2003, 3823, 4313], 'hand,': [2006, 4316], 'solute': [2011, 2227], 'transporters': [2012], 'little': [2017, 2216], 'attention.': [2018], 'Considering': [2019, 4335, 4466], 'lipid': [2022, 4245], 'composition': [2023, 4246], '(34van': [2027, 4201], 'de': [2028, 2047, 2122, 4202, 4221], 'Vossenberg': [2029, 2048, 4203, 4222], 'Driessen': [2031, 2050, 2125, 2199, 4205, 4224], 'A.J.': [2032, 2126, 4206], 'Konings': [2033, 2052, 2197, 4207, 4226], 'W.N.': [2034, 2115, 4208], 'Extremophiles.': [2035, 4209], '2:': [2037, 4211], '163-170Crossref': [2038, 4212], '(174)': [2041, 4215], '35Albers': [2044, 4218], 'van': [2046, 4220], 'D813-D820Crossref': [2058, 4232], 'could': [2065, 4398], 'hypothesized': [2067], 'should': [2072], 'characteristics.': [2078], 'although': [2080], 'A1A0-ATPases': [2082], '(the': [2083], 'archaeal': [2084, 4255], 'homologs': [2085], 'F1F0-ATPases),': [2087], 'redox,': [2088], 'electron': [2090], 'components': [2092], 'studied': [2097], '(36Schafer': [2098], 'Engelhard': [2100], 'Muller': [2102], 'Microbiol.': [2104, 2202], '63:': [2109], '570-620Crossref': [2110], '37Konings': [2114], 'Tolner': [2116], 'Speelmans': [2118], 'Elferink': [2120], 'Wit': [2123], 'J.G.': [2124], 'Bioenerg.': [2128], 'Biomembr.': [2129], '24:': [2131], '601-609Crossref': [2132], '(20)': [2135], 'sugar': [2139], 'transporter': [2140, 4256], 'ABC': [2141], 'Thermococcus': [2144], 'litoralis': [2145], '(38Greller': [2146], 'Horlacher': [2148], 'DiRuggiero': [2150], 'Boos': [2152, 2178], '20259-20264Abstract': [2159], '(57)': [2167], '39Xavier': [2170], 'Martins': [2172], 'Peist': [2174], 'Kossmann': [2176], 'Santos': [2180], '178:': [2185], '4773-4777Crossref': [2186], 'Scholar)': [2189, 2210, 2422, 2572, 3873, 3919], 'Sulfolobus': [2191], 'acidocaldarius': [2192], '(40Elferink': [2193], 'Albers': [2195], '39:': [2204], '1494-1503Crossref': [2205], '(117)': [2208], 'initially': [2213], 'characterized.': [2214], 'proteins.': [2229, 2263], 'We': [2230, 3719, 3978], 'initiated': [2232, 3164, 3979], 'goal': [2240], 'mechanism,': [2245], 'features,': [2247], 'mechanisms': [2249], 'while': [2256], 'gaining': [2257], 'report,': [2266], 'CPx-ATPase': [2279], 'Archaeoglobus': [2284], 'fulgidus.': [2285], 'biochemical': [2297], 'Ag+/Cu+-ATPase.': [2302], 'cDNA': [2304, 2446, 3988], 'geneAF0473': [2306], 'genome': [2311, 3597], '(41Klenk': [2312, 3608], 'Clayton': [2314, 3610], 'Tomb': [2316, 3612], 'White': [2318, 3614], 'O.': [2319, 3615], 'Nelson': [2320, 3616], 'Ketchum': [2322, 3618], 'Dodson': [2324, 3620], 'Gwinn': [2326, 3622], 'Hickey': [2328, 3624], 'E.': [2329, 2351, 3625, 3647, 3994], 'Peterson': [2330, 2360, 3626, 3656], 'Richardson': [2332, 3628], 'Kerlavage': [2334, 3630], 'Graham': [2336, 3632], 'Kyrpides': [2338, 3634], 'N.': [2339, 2345, 3635, 3641], 'Fleischmann': [2340, 3636], 'Quackenbush': [2342, 3638], 'Lee': [2344, 3640], 'Sutton': [2346, 3642], 'Gill': [2348, 3644], 'Kirkness': [2350, 3646], 'Dougherty': [2352, 3648], 'McKenney': [2354, 3650], 'Adams': [2356, 3652], 'Loftus': [2358, 3654], 'Reich': [2362, 3658], 'McNeil': [2364, 3660], 'Badger': [2366, 3662], 'Glodek': [2368, 3664], 'Zhou': [2370, 3666], 'Overbeek': [2372, 3668], 'Gocayne': [2374, 3670], 'Weidman': [2376, 3672], 'McDonald': [2378, 3674], 'Utterback': [2380, 3676], 'Cotton': [2382, 3678], 'Spriggs': [2384, 3680], 'Artiach': [2386, 3682], 'P.': [2387, 2393, 3683, 3689, 3899], 'Kaine': [2388, 3684], 'Sykes': [2390, 3686], 'Sadow': [2392, 3688], "D'Andrea": [2394, 3690], 'Bowman': [2396, 3692], 'Fujii': [2398, 3694], 'Garland': [2400, 3696], 'Mason': [2402, 3698], 'Olsen': [2404, 3700], 'Fraser': [2406, 3702], 'Woese': [2410, 3706], 'Venter': [2412, 3708], '390:': [2416, 3712], '364-370Crossref': [2417, 3713], '(1215)': [2420, 3716], 'generated': [2424], 'polymerase': [2426], 'chain': [2427], 'reaction,': [2428], 'genomic': [2430], 'DNA': [2431, 2480], '(ATCC,': [2432], 'Manassas,': [2433], 'VA)': [2434], 'template': [2436], 'oligonucleotides': [2439], '5′-GTAAAGGATACTTATATCTCTTCAGCCAGC-3′': [2440], '5′-GCTTCTGAGCTTTGCCTGGTATCC-3′.': [2442], 'resulting': [2444], '2412-bp': [2445], 'subcloned': [2448], 'pBADTOPO/His': [2450], 'vector': [2451, 2456], '(Invitrogen,': [2452, 2487, 2941], 'Carlsbad,': [2453, 2488, 2942], 'CA).': [2454, 2943], 'introduces': [2457], 'carboxyl-terminal': [2459], 'hexahistidine': [2460], 'tag': [2461], 'suitable': [2462], 'Ni2+': [2464], 'purification': [2466, 2538, 2577, 2894], 'V5': [2469], 'epitope': [2470], 'immunodetection.': [2472], 'automated': [2479], 'analysis.': [2482], 'Escherichia': [2483], 'coli': [2484], 'Top10': [2485], 'cells': [2486, 2647], 'CA)': [2489, 2774], 'were': [2490, 2517, 2579, 2589, 2596, 2648, 2686, 2724, 2827, 2873, 2913, 2994, 3004, 3191, 3244, 3276, 3291, 3315, 3337, 3449, 3514, 3524, 3552, 4326], 'transformed': [2491], 'construct': [2494], 'grown': [2496], '2×': [2501], 'YT': [2502], 'medium': [2503, 3118, 3263, 4169, 4397], 'supplemented': [2504], '150': [2506], 'μg/ml': [2507], 'ampicillin,': [2508], 'induced': [2512], '0.002%': [2514], 'l-arabinose.': [2515], 'Cells': [2516, 2595], 'harvested': [2518], '3': [2519, 2959, 2961, 4077], 'h': [2520, 2748, 2793], 'postinduction;': [2521], 'washed': [2522, 2672, 2805, 3201], '25': [2524, 2709, 2845, 3130, 4430], 'mm': [2525, 2530, 2602, 2607, 2610, 2634, 2710, 2715, 2718, 2721, 2812, 2824, 2846, 2851, 2854, 2859, 2951, 2962, 2965, 2972, 2983, 3121, 3128, 3133, 3142, 3187, 3253, 3257, 4370], 'Tris,': [2526, 2603, 2711, 2847, 2952, 3122], 'pH': [2527, 2604, 2712, 2848, 2953, 3007, 3013, 3123, 4443, 4449], '7.0,': [2528, 2605], '100': [2529, 2606, 2714, 2850, 2980, 2986, 3152, 3158, 4317], 'KCl;': [2531], 'stored': [2533, 2694, 2863], '−70': [2535, 2696, 2868], 'Protein': [2537, 2891], 'carried': [2540, 2580, 3114], 'out': [2541, 2581, 3115], 'following': [2542, 3534, 3562], 'method': [2544], 'et': [2547, 3071], 'al.': [2548, 3072], '(20Sharma': [2549], 'minor': [2574], 'modifications.': [2575], 'All': [2576, 2870], 'procedures': [2578], '0–4': [2583], '°C,': [2584, 2796, 4476], 'special': [2587], 'precautions': [2588], 'taken': [2590], 'prevent': [2592], 'oxidation.': [2594], 'suspended': [2597], 'buffer': [2599, 2674, 2689, 2707, 2777, 2807, 2819, 2866], '(25': [2601], 'sucrose,': [2608, 2716, 2852], '1': [2609, 2666, 2683, 2720, 2747, 2766, 2792, 2858, 3127, 3186, 3252, 3256, 3539], 'phenylmethylsulfonyl': [2611, 2722], 'fluoride)': [2612, 2723], 'disrupted': [2614], 'passing': [2616], 'them': [2617], 'through': [2618, 3193], 'French': [2620], 'press': [2621], '20,000': [2623], 'p.s.i.': [2624], 'After': [2625, 2789], 'addition': [2627, 3167], '0.02': [2629], 'mg/ml': [2630, 2705, 2975, 3147], 'DNase': [2631], 'I,': [2632], '2': [2633, 3547], 'MgCl2,': [2635, 3129], 'homogenate': [2637], 'incubated': [2639, 2742, 3277], '30': [2641, 2654, 3173, 3265], 'min': [2642, 3059, 3371, 3537], '4': [2644, 2750, 2795, 3459, 4452, 4455], 'Lysed': [2646], 'centrifuged': [2649, 2660, 2677], '8000': [2651], '×g': [2652], 'min.': [2655], 'supernatant': [2657], 'then': [2659], '163,000': [2662], '×': [2663, 2680, 2763], 'g': [2664, 2681, 2764], 'h,': [2667, 2767], 'pellet': [2670], '229,000': [2679, 2762], 'h.': [2684], 'Membranes': [2685], 'resuspended': [2687], '(10–15': [2691], 'mg/ml)': [2692], 'solubilization': [2700], '(3': [2704], 'B:': [2708], '8.0,': [2713, 2849], '500': [2717], 'NaCl,': [2719, 2855, 2973, 3143], 'treated': [2725], 'dodecyl-β-d-maltoside': [2727], '(DDM)': [2728], '(Calbiochem),': [2729], 'added': [2730, 2785, 3260], 'dropwise': [2731], 'final': [2734], 'concentration': [2735, 3328, 3355, 3362, 4361, 4408], '0.75%.': [2737], 'preparation': [2740], 'detergent': [2745, 4165], 'mild': [2753], 'agitation.': [2754], 'suspension': [2756], 'cleared': [2758], 'centrifugation': [2760], 'Ni2+-nitrilotriacetic': [2769, 4067], 'resin': [2771, 2798], '(Qiagen,': [2772], 'Valencia,': [2773], 'pre-equilibrated': [2775], 'B': [2778], 'plus': [2779], '0.05%': [2780, 2809, 2821], 'DDM,': [2781, 2810, 2822, 2857, 2970, 3140], '5': [2782, 3179, 4013], 'mmimidazole': [2783], 'supernatant.': [2788], 'incubation': [2790], 'placed': [2800], 'column': [2803, 4070], 'B,': [2808, 2820], '20': [2811, 2964, 3135], 'imidazole.': [2813, 2825], 'eluted': [2817, 2843], '300': [2823], 'Fractions': [2826], 'concentrated': [2828], 'filtration,': [2830], 'imidazole': [2832], 'removed': [2834], 'Sephadex': [2837], 'G-25': [2838], 'column.': [2839], '50': [2853, 2950, 3120], '0.01%': [2856, 2967, 2969, 3137, 3139], '(DTT)': [2861], 'performed': [2874, 3443, 3493], 'accordance': [2876, 3068], 'Bradford': [2878], '(42Bradford': [2879], 'M.M.': [2880], 'Anal.': [2881, 3081], '1976;': [2883], '72:': [2884], '248-254Crossref': [2885], '(223660)': [2888], 'examined': [2896, 3245], '10%': [2898, 3183], 'SDS-PAGE': [2899, 4085], '(43Laemmli': [2900], 'U.K.': [2901], '1970;': [2903], '227:': [2904], '680-685Crossref': [2905], '(215642)': [2908], 'bands': [2912], 'staining': [2916], 'gels': [2918, 2930, 4086], 'Coomassie': [2920, 4082], 'Brilliant': [2921, 4083], 'Blue.': [2922], 'Heterologous': [2923], 'detected': [2926], 'electroblotting': [2928], 'onto': [2931], 'nitrocellulose': [2932, 3194], 'immunostaining': [2935], 'anti-V5-horseradish': [2937], 'peroxidase': [2938], 'monoclonal': [2939], 'antibodies': [2940], 'assay': [2947, 4168], 'mixture': [2948], 'contained': [2949], '6.1': [2954], '°C)': [2956], '(see': [2957], 'below),': [2958], 'mmMgCl2,': [2960], 'cysteine,': [2966], 'asolectin,': [2968, 3138], '400': [2971, 3141], '0.01': [2974], 'enzyme,': [2977, 3149], 'μmCu2SO4,': [2981], '2.5': [2982, 3155], 'DTT,': [2984], 'μm': [2987, 3153, 3159, 4318], 'AgNO3.': [2988, 3160], 'different': [2990], 'experiments,': [2991], 'reagents': [2993], 'independently': [2995], 'varied': [2996], 'indicated': [2998, 3517], 'corresponding': [3001], 'figures.': [3002], 'Buffers': [3003], 'prepared,': [3005], 'measured': [3009, 3056, 3093, 3211, 3218, 4307], 'room': [3011], 'temperature.': [3012, 4465], '75': [3015, 3061, 4295, 4487], 'calculated': [3018, 3383], 'pK': [3020], '/': [3022], 'conversion': [3024], 'factors': [3025], '(Tris,': [3026], '−0.031;': [3027], 'MES,': [3028], '−0.011;': [3029], 'CHES,': [3030], '−0.009)': [3031], '(44Good': [3032], 'N.E.': [3033], 'Winget': [3034], 'G.D.': [3035], 'Winter': [3036], 'Connolly': [3038], 'T.N.': [3039], 'Izawa': [3040], 'Singh': [3042], 'R.M.': [3043], '1966;': [3045], '467-477Crossref': [3047], '(2191)': [3050], '10': [3058, 4410], 'Released': [3063], 'Pi': [3064], 'Lanzetta': [3070], '(45Lanzetta': [3073], 'P.A.': [3074], 'Alvarez': [3075], 'L.J.': [3076], 'Reinach': [3077], 'P.S.': [3078], 'Candia': [3079], 'O.A.': [3080], '1979;': [3083], '100:': [3084], '95-97Crossref': [3085], '(1877)': [3088], 'Background': [3091, 3216], 'activity,': [3092], 'absence': [3096, 3221], 'metals,': [3099, 3224], 'less': [3101, 3226], '5%': [3103], 'our': [3107, 3980, 4512], 'determinations.': [3108], 'Enzyme': [3109, 3409, 4047], '7.5': [3124], '(20': [3125], '°C),': [3126], 'μm[γ-32P]ATP,': [3131], '0.04': [3132], 'EGTA,': [3134], 'mmcysteine,': [3136], '20%': [3144], 'Me2SO,': [3145], '0.05': [3146], 'Cu2SO4,': [3154], 'mmDTT,': [3156], 'reaction': [3162], '[γ-32P]ATP': [3169], 'stopped': [3171, 3292], 's': [3174, 3266, 3285], 'volumes': [3180], 'ice-cold': [3182], 'trichloroacetic': [3184], 'acid,': [3185, 3294], 'Pi.': [3188], 'samples': [3190, 3247, 3275], 'filtered': [3192], '0.45-μm': [3195], 'filters': [3196], '(Millipore': [3197], 'Corp.,': [3198], 'Bedford,': [3199], 'MA),': [3200], 'times': [3203], 'stopping': [3206, 3271], 'solution,': [3207, 3272], 'radioactivity': [3209], 'scintillation': [3214], 'counter.': [3215], 'phosphorylation,': [3217], '5%.': [3228], 'time': [3230], 'course': [3231], 'dephosphorylation': [3234], 'effect': [3237, 4053, 4375], 'ADP': [3239, 3254], 'level': [3243], 'above.': [3251, 3300], 'instead': [3267], 'further': [3280], '3,': [3281], '6,': [3282], '9': [3284], 'incubations': [3290], 'filtered,': [3295], 'counted': [3297], 'Curves': [3301], 'versusAg+,': [3305], 'well': [3310], 'curves,': [3314], 'fit': [3316, 3338], 'v': [3318, 3340], '=V': [3319], 'L/(L': [3321], '+K': [3322], '12),': [3323], 'where': [3324, 3351, 4027], 'L': [3325], 'variable': [3330], 'ligand.': [3331], 'versus': [3334], 'inhibitors': [3335], 'curves': [3336], '=(V': [3341], '−V': [3343], 'min)/(1': [3344], '+': [3345, 3348], '(I/K': [3346], '12))': [3347], 'min,': [3350], 'I': [3352], 'inhibitor,K': [3357], 'inhibitor': [3361], 'produces': [3364], 'half': [3365], 'inhibitory': [3367], 'effect,': [3368], 'maximum': [3376, 4299, 4446, 4484], 'inhibition.': [3377], 'Activation': [3378], '(E': [3380, 3394], 'a)': [3381], 'fitting': [3385, 3483], 'experimental': [3387, 4513], 'data': [3388, 3513, 3523, 3551], 'log': [3390], '/2.3R)/(1/T),': [3396], 'whereR': [3397], 'gas': [3400], 'constant': [3401], 'T': [3403], 'absolute': [3405], 'temperature': [3406, 4355], '(46Segel': [3407], 'I.H.': [3408], 'Kinetics.': [3410], 'John': [3411], 'Wiley': [3412], '&': [3413], 'Sons,': [3414], 'Inc.,': [3415], 'York1993Google': [3417], 'Data': [3419], 'done': [3422], 'KaleidaGraph': [3425], 'software': [3426, 3496], '(Synergy,': [3427], 'Reading,': [3428], 'PA).': [3429], 'Experimental': [3430], 'values': [3431, 3467, 3479], 'mean': [3434], '±': [3435, 3540, 3546, 3567, 3573], 'S.E.': [3436, 3466, 3478], 'least': [3439], 'independent': [3441, 4377], 'experiments': [3442], 'duplicate.': [3445], 'simplicity,': [3447], 'errors': [3448], 'included': [3451], 'Fig.4': [3453], 'A,': [3454], 'inset': [3456], 'Fig.': [3458, 3462, 4454], 'C,': [3460], '6.': [3463], 'max,K': [3470], 'm,': [3471], '12,': [3473], 'andE': [3474], 'asymptotic': [3477], 'program.': [3484], 'Sequence': [3485], 'percentage': [3488], 'identity': [3489], '(ClustalV': [3490], 'method)': [3491], 'LaserGene': [3495], '(DNASTAR,': [3497], 'Madison,': [3498], 'WI).Figure': [3499], '6CopA': [3500], 'inhibition': [3501], 'oligomycin.': [3505], 'Ag-ATPase': [3508, 4387], 'determined,': [3511], 'fitted': [3515, 3525, 3553], '"Experimental': [3519], 'Procedures."': [3520], 'Vanadate': [3521], '(●)': [3522], '100%': [3530, 3558, 3576], 'yielded': [3532, 3560, 4092, 4385], 'parameters:': [3535, 3563], '3%,': [3541], 'μm.': [3548, 3575], 'Oligomycin': [3549], '(○)': [3550], 'min=': [3565], '55': [3566], '2%,': [3568], '4.7': [3572], '0.7': [3574], '12–14.5': [3579], 'μmol/mg/h.View': [3580], 'Large': [3581], 'Image': [3582], 'Figure': [3583], 'ViewerDownload': [3584], 'Hi-res': [3585], 'image': [3586], 'Download': [3587], '(PPT)': [3588], 'hyperthermophylic': [3593], 'sulfur-metabolizing': [3594], 'organism.': [3595], 'Its': [3596], 'genes': [3604], 'AF0473': [3605], 'AF0152': [3607], 'refer': [3721], 'AAB90763)': [3730], '(this': [3731], 'previously': [3734], 'referred': [3735], 'PacS)': [3737], 'CopB': [3739, 3826, 3851], 'AAB91079).': [3743], '804': [3747], '690': [3749], 'acids,': [3751], 'respectively,': [3752], 'typical': [3755], 'pattern': [3757], '(i.e.': [3761], 'large': [3766], 'loop': [3768], 'sequence)': [3772], 'homology': [3778, 4269], 'they': [3780], 'closely': [3782], 'related': [3783], 'Cu+/Ag+': [3786], 'transporting': [3787], 'subgroup': [3788], '(47Rensing': [3793], 'Ghosh': [3795], '181:': [3802], '5891-5897Crossref': [3803], 'single': [3810], 'GMTCAMC': [3811], 'N-MBD': [3812, 3830], 'CPC': [3815], 'sixth': [3821, 3840], 'TM.': [3822, 3841], 'contrary,': [3825], '(1MHEHDSHGEAAHSNSEDMQMIHQHHEHHGHEEEHSAHHEKMKHSADHGDHHRM53)': [3831], 'CPH': [3834], 'structures': [3843], 'fromEnterococcus': [3852], 'hirae': [3853, 3897], '(48Odermatt': [3854], 'Suter': [3856], 'Krapf': [3858], 'Solioz': [3860, 3906], '268:': [3866], '12775-12779Abstract': [3867], '(percentage': [3874], 'identity,': [3875], '41': [3876], '40%,': [3878], 'respectively).': [3879], 'systems': [3884], 'probably': [3886], 'complemented': [3887], 'copper': [3891], 'chaperone,': [3892], 'CopZ': [3895], 'fromE.': [3896], '(49Cobine': [3898], 'Wickramasinghe': [3900], 'W.A.': [3901], 'Harrison': [3902], 'M.D.': [3903], 'Weber': [3904], 'Dameron': [3908], 'FEBS': [3910], 'Lett.': [3911], '445:': [3913], '27-30Crossref': [3914], '(139)': [3917], 'Atx1': [3921], '(50Lin': [3924], 'S.J.': [3925], 'Pufahl': [3926], 'R.A.': [3927], 'Dancis': [3928], 'A.,': [3929], 'TV,': [3930], 'O.H.': [3931], 'Culotta': [3932], 'V.C.': [3933], '9215-9220Abstract': [3939], '(359)': [3947], 'geneAF0346': [3952], '(51Jordan': [3956], 'Natale': [3958], 'Galperin': [3960], '25:': [3966], '480-481Abstract': [3967], '(14)': [3975], 'cloning': [3986], 'expressing': [3990], 'coli.': [3995], 'Fig.2': [3996], 'shows': [3997, 4087, 4457], 'isolated': [4003], 'induction': [4006], 'arabinose': [4010], '2,lanes': [4012], '6).': [4015, 4079], 'To': [4016], 'isolate': [4017], 'protein,': [4019], 'solubilized': [4022, 4061], 'DDM': [4024], 'conditions': [4026, 4470], 'retained.': [4030], 'Treatment': [4031], 'detergents': [4034], '(Triton': [4035], 'X-100,': [4036], 'C12E8,': [4037], 'Tween': [4038], '20)': [4039], 'concentrations': [4042, 4427], 'led': [4043, 4420], 'inactivation.': [4046], 'ligands': [4048], '(Mg-ATP)': [4049], 'had': [4050], 'solubilization.': [4059], 'subsequently': [4064], 'standard': [4072], 'protocols': [4073], '2,': [4075, 4098], 'lanes': [4076], 'Densitometry': [4080], 'Blue-stained': [4084], 'procedure': [4090], 'routinely': [4091], '>90%': [4094], 'pure': [4095], 'lane': [4099], '3).': [4100, 4334], 'As': [4101, 4264], 'case': [4104], 'detergent-solubilized': [4107], '52Vilsen': [4127], 'Andersen': [4129], 'J.P.': [4130], 'Eur.': [4131], '1987;': [4134], '170:': [4135], '421-429Crossref': [4136], '(21)': [4139], '53Esmann': [4142], '815:': [4148], '196-202Crossref': [4149], '(16)': [4152], 'inclusion': [4158], 'optimized': [4160], 'lipids': [4163], 'functional.': [4172], 'asolectine': [4177], '(phosphatidyl': [4178], 'choline-enriched': [4179], 'soybean': [4180], 'lipids)': [4181], 'able': [4183], 'replace': [4185], 'isoprenoid': [4187], 'dialkyl': [4188], 'diglycerol': [4189], 'cyclic': [4190], 'tetraethers': [4191], 'predominantly': [4193], 'constitute': [4194], 'monolayer': [4196], 'influence': [4243], 'kinetics': [4252], 'beyond': [4258], 'scope': [4260], 'experiments.': [4263], 'expected': [4265], 'based': [4266], 'monovalent': [4272], 'living': [4278], 'environment': [4279], 'fulgidus,': [4282], 'showed': [4286, 4435, 4498], 'Ag+-': [4287], 'Cu+-stimulated': [4289], 'when': [4292], 'assayed': [4293], '(Fig.3).': [4297], 'Cu-ATPase': [4300], '25%': [4304], 'Ag+.': [4312], 'Cu2+,': [4319], 'Hg2+,': [4320], 'Fe2+,': [4323], 'Co2+': [4325], 'significantly': [4329], 'activate': [4330], 'peculiarities': [4338], 'extremophile': [4342], 'dependence': [4345, 4439, 4459], 'silver-stimulated': [4347], 'ionic': [4351, 4393], 'strength,': [4352], 'pH,': [4353], 'analyzed': [4357], '(Fig.4).': [4358], 'High': [4359], 'salt': [4360, 4371], 'stimulated': [4362, 4412], 'ATPase,': [4364], 'reaching': [4365], 'plateau': [4367], '400–500': [4369], '(Fig.4': [4372], 'A).': [4373], 'cation,': [4380], 'NaCl': [4382, 4416], 'KCl': [4384], 'stimulation.': [4388], 'Experiments': [4389], 'usingN-methylglucamine': [4390], 'increase': [4392], 'strength': [4394], 'confirm': [4400], 'point.': [4402], 'amine': [4405], 'low': [4407], '(under': [4409], 'mm)': [4411, 4431], 'KCl,': [4418], 'sharp': [4423], '(IC50': [4428], '(not': [4432], 'shown).': [4433], 'typical,': [4437], 'bell-shaped,': [4438], '6.1–6.5': [4450], 'B).': [4453], 'C': [4456], 'physiological': [4469], 'works': [4473], '70–80': [4475], 'surprising': [4480], 'An': [4495], 'Arrhenius': [4496], 'plot': [4497], 'kJ/mol': [4505], 'conditio': [4514]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2030789678', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 6}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 7}, {'year': 2016, 'cited_by_count': 6}, {'year': 2015, 'cited_by_count': 6}, {'year': 2014, 'cited_by_count': 8}, {'year': 2013, 'cited_by_count': 7}, {'year': 2012, 'cited_by_count': 9}], 'updated_date': '2025-01-08T15:56:03.295131', 'created_date': '2016-06-24'}
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