Title: An in vivo effect of the metabolites l-alanine and glycyl-l-leucine on the properties of the lysyl-tRNA synthetase from Escherichia coli K-12 II. Kinetic evidence
Abstract: Wild-type Escherichia coli K-12 was grown in minimal medium alone or with the addition of 20 mM l-alanine or 3 mM glycyl-l-leucine. A lysyl-tRNA synthetase mutant strain was grown in minimal medium containing 20 mM l-alanine. The lysyl-tRNA synthetase from these strains was purified to 70–90% of homogeneity. Kinetic studies comparing the effect of thermal and urea inactivation on these different lysyl-tRNA synthetase preparations and measurement of the Michaelis constant for lysine and transfer RNA indicates that growth of Escherichia coli in the presence of alanine and glycyl-l-leucine induces an alteration in the properties of the synthetase. Measurement of the apparent Km for ATP at pH 7.25 indicates lysyl-tRNA synthetase has two binding sites for this substrate, and further studies indicated a dependence of the apparent Km for lysine on the ATP concentration.
Publication Year: 1976
Publication Date: 1976-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 12
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