Title: Accelerated chromatographic method for determination of hydroxyproline
Abstract: An automated method is described for the determination of urinary hydroxyproline. The method utilizes accelerated column chromatography on spherical sulfonated polystyrene resin, PA-35, and a Beckman/Spinco model 116 amino acid analyzer equipped with sample injection and high sensitivity recording. The effluent is monitored by ninhydrin analysis. The method requires only 30 min per analysis, and allows better than 98% reproducibility and recovery. Moreover, compared to other methods, less handling is required for each sample, and less time is required of the analyst. Eight normal subjects, whose sole source of collagen was derived from a controlled diet containing 10 oz of meat per day, excreted 38.71 mg of hydroxyproline per 24 hr. After correcting for the urinary hydroxyproline excretion arising from dietary collagen, the same urinary excretion of hydroxyproline was obtained as that reported by others for subjects on collagen-free diets.
Publication Year: 1972
Publication Date: 1972-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 6
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