Title: Stress-induced Ethylene Biosynthesis in Pine Needles: A Search for the putative 1 -aminocyclopropane-l-carboxylic Acid-Independent Pathway
Abstract: A possible involvement of an alternative, 1-aminocyclopropane-l-carboxylic acid (ACC) independent pathway of ethylene biosynthesis in ethylene evolution from stressed pine (Pinus sylvestris L.) needles was investigated. Inhibitors of ethylene biosynthesis (aminoethoxyvinylglycine and aminooxyacetic acid) and lipid peroxidation (selenomethionine) were applied to establish whether they can be used to distinguish between different pathways of ethylene production. Ethylene biosynthesis in detached pine needles was initiated by induction of ACC synthesis. Exogenous H202 and Na2S205 induced increases in ethylene production in detached needles in a concentration-dependent manner. Aminooxyacetic acid completely prevented an increase in ACC content in detachment-stressed needles and by 90% and 80% inhibited that in H202 and Na2S205-treated ones. However, a significant portion of ethylene produced by stressed needles was insensitive to the inhibitor. Cycloheximide completely inhibited H202-induced ethylene production. Based on aminooxyacetic acid and selenomethionine experiments, it is possible to exclude a participation of an ACC-independent pathway in ethylene production by stressed pine needles, with only the exception of that induced by high concentrations of Na2S205. The data collectively indicate that the residual rate of ethylene production from plant tissues in the presence of inhibitors does not reflect an ACC-independent pathway.
Publication Year: 1995
Publication Date: 1995-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 23
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