Publication Information

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'biblio': {'volume': '277', 'issue': '49', 'first_page': '46987', 'last_page': '46992'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10915', 'display_name': 'Trace Elements in Health', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2916', 'display_name': 'Nutrition and Dietetics'}, 'field': {'id': 'https://openalex.org/fields/29', 'display_name': 'Nursing'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10915', 'display_name': 'Trace Elements in Health', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2916', 'display_name': 'Nutrition and Dietetics'}, 'field': {'id': 'https://openalex.org/fields/29', 'display_name': 'Nursing'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10570', 'display_name': 'Drug Transport and Resistance Mechanisms', 'score': 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'display_name': 'Wild type', 'score': 0.41675237}], 'concepts': [{'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.76855683}, {'id': 'https://openalex.org/C2781116636', 'wikidata': 'https://www.wikidata.org/wiki/Q426228', 'display_name': 'Vanadate', 'level': 2, 'score': 0.72150433}, {'id': 'https://openalex.org/C23265538', 'wikidata': 'https://www.wikidata.org/wiki/Q300033', 'display_name': 'ATPase', 'level': 3, 'score': 0.69186425}, {'id': 'https://openalex.org/C87746105', 'wikidata': 'https://www.wikidata.org/wiki/Q11903647', 'display_name': 'P-type ATPase', 'level': 4, 'score': 0.6808927}, {'id': 'https://openalex.org/C2779201268', 'wikidata': 'https://www.wikidata.org/wiki/Q186474', 'display_name': 'Cysteine', 'level': 3, 'score': 0.6751619}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 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[2, 13, 70, 88, 196, 200, 211, 268, 286, 394, 398, 445, 642, 896, 988, 1002, 1014, 1118, 1124, 1139, 1809, 2557, 2785, 2911, 3112, 3208, 3268, 3336, 3348, 3354, 3415, 3419, 3453, 3492, 3509, 3538, 3565, 3636, 3693, 3827, 3853, 3958, 4174, 4218, 4285, 4442, 4470, 4602, 4762, 4882, 4891, 4974, 5011, 5102, 5209, 5240, 5305], 'is': [3, 65, 201, 263, 424, 648, 796, 882, 920, 962, 979, 992, 1393, 1562, 1867, 2726, 2912, 3032, 3182, 3209, 3234, 3269, 3317, 3394, 3399, 3609, 3834, 4040, 4050, 4057, 4171, 4205, 4247, 4367, 4425, 4542, 4603, 4709, 4821, 4856, 4997, 5017, 5092, 5098, 5234, 5241, 5259, 5276], 'a': [4, 15, 40, 98, 202, 213, 238, 296, 425, 448, 751, 945, 974, 1005, 1030, 1113, 1152, 1172, 1279, 1333, 1370, 1500, 1690, 1722, 1838, 1973, 2125, 2154, 2263, 2399, 2409, 2478, 2580, 2622, 2746, 2783, 2791, 2812, 2913, 2978, 3054, 3190, 3210, 3214, 3384, 3443, 3502, 3568, 3573, 3599, 3610, 3657, 3675, 3929, 3971, 4063, 4081, 4142, 4176, 4254, 4605, 4632, 4741, 4764, 4778, 4815, 5178, 5202, 5219], 'copper': [5, 11, 139, 170, 203, 209, 337, 368, 399, 645, 651, 924, 953, 1102, 1143, 2914, 2926, 2932, 2960, 3273, 3285, 3579, 3703, 3763, 3786, 3823, 3844, 3975, 4088, 4370, 4452, 4458, 4472, 4579, 4682, 4707, 4785, 4800, 4831, 4842, 4852, 4912], 'ion-translocating': [6, 204, 5022, 5269], 'P-type': [7, 63, 205, 261, 432, 581, 684, 971, 1052, 2724, 2887, 3219, 3227, 4239, 4281, 4612, 4913, 5270], 'ATPase': [8, 64, 78, 206, 262, 276, 1053, 2617, 3847, 4028, 4055, 4069, 4097, 4169, 4185, 4214, 4282, 4613], 'that': [9, 57, 179, 207, 255, 377, 450, 597, 693, 782, 909, 958, 978, 1049, 1109, 1406, 1817, 2542, 2727, 2963, 2977, 3116, 3180, 3207, 3223, 3254, 3370, 3381, 3398, 4052, 4084, 4166, 4178, 4241, 4369, 4427, 4530, 4540, 4797, 4822, 4844, 4999, 5016, 5096, 5235, 5247, 5300], 'confers': [10, 208], 'resistance.': [12, 210], 'formed': [14, 134, 212, 332, 3530], 'phosphorylated': [16, 214, 980, 1006, 2787, 3569, 3600, 4742, 4759, 5167], 'intermediate': [17, 215, 1007, 2749, 2824, 3412, 3533, 3760], 'with': [18, 32, 80, 121, 216, 230, 278, 319, 1008, 1019, 1024, 1127, 1225, 1235, 1381, 1441, 1474, 1532, 1564, 1646, 1755, 1999, 2018, 2251, 2259, 2323, 2338, 2353, 2394, 2408, 2485, 2495, 2520, 2584, 2592, 2606, 2715, 2788, 2794, 2988, 3047, 3483, 3493, 3495, 3501, 3511, 3524, 3584, 3761, 3933, 3954, 4006, 4100, 4136, 4147, 4358, 4671, 4763, 5218, 5249, 5261], '[γ-32P]ATP.': [19, 217, 1009, 4761, 5169], 'Phosphorylation': [20, 38, 218, 236, 3417, 4350, 4436, 4464], 'was': [21, 111, 219, 309, 1015, 1125, 1140, 1238, 1344, 1366, 1403, 1458, 1483, 1517, 1537, 1753, 1814, 1831, 1841, 1850, 1946, 2105, 2122, 2133, 2144, 2182, 2195, 2248, 2277, 2296, 2406, 2448, 2461, 2475, 2493, 2518, 2574, 2619, 2702, 2786, 2825, 2891, 2900, 2937, 2981, 3058, 3087, 3130, 3138, 3154, 3420, 3678, 3765, 3828, 3875, 3959, 3980, 3993, 4013, 4031, 4074, 4109, 4128, 4351, 4476, 4482, 4493, 4548, 4589, 4758, 4772, 4777, 5166], 'inhibited': [22, 220, 1148, 2902, 4032, 4042, 4075], 'by': [23, 113, 221, 311, 981, 1131, 1142, 1149, 1209, 1351, 1378, 1408, 1460, 1519, 1554, 1732, 1746, 1822, 1847, 1869, 1948, 2011, 2146, 2206, 2236, 2298, 2314, 2463, 2504, 2558, 2576, 2621, 2853, 2903, 2925, 3799, 3961, 4034, 4043, 4062, 4079, 4113, 4173, 4484, 4550, 4621, 4760, 5168], 'vanadate': [24, 222], 'and': [25, 29, 67, 95, 97, 118, 173, 223, 227, 265, 293, 295, 316, 371, 491, 547, 594, 620, 647, 653, 750, 903, 955, 1057, 1105, 1129, 1147, 1197, 1227, 1261, 1268, 1296, 1360, 1383, 1385, 1411, 1413, 1452, 1470, 1488, 1512, 1526, 1542, 1571, 1605, 1630, 1706, 1710, 1744, 1770, 1837, 1940, 1959, 1990, 2007, 2061, 2070, 2079, 2091, 2102, 2141, 2233, 2257, 2286, 2341, 2438, 2457, 2539, 2548, 2560, 2571, 2830, 3066, 3076, 3122, 3199, 3264, 3287, 3325, 3541, 3639, 3649, 3710, 3726, 3776, 3880, 3940, 3957, 3978, 3987, 4154, 4156, 4355, 4376, 4403, 4434, 4439, 4467, 4532, 4539, 4558, 4631, 4646, 4698, 4720, 4737, 5138, 5215, 5246, 5317], 'sensitive': [26, 224, 1016, 2826, 3351, 4352, 5150, 5212], 'to': [27, 147, 225, 345, 447, 592, 600, 688, 884, 886, 1003, 1017, 1336, 1447, 1507, 1632, 1634, 1757, 1816, 2116, 2124, 2184, 2213, 2294, 2711, 2827, 2837, 2922, 3042, 3101, 3104, 3107, 3185, 3340, 3369, 3427, 3535, 3558, 3566, 3647, 3690, 3724, 3737, 3782, 3810, 3836, 3908, 3982, 4008, 4059, 4076, 4130, 4207, 4353, 4400, 4451, 4462, 4508, 4517, 4574, 4687, 4739, 4801, 4832, 4890, 4982, 5027, 5094, 5111, 5142, 5151, 5213], 'KOH': [28, 226, 2285], 'hydroxylamine,': [30, 228, 1022, 4356], 'consistent': [31, 229, 1023, 4357, 5260], 'acylphosphate': [33, 135, 231, 333, 1025, 2748, 2846, 2917, 3128, 3532, 3759, 4252, 4359, 5226, 5279], 'formation': [34, 232, 1028, 2764, 2899, 2918, 3129, 3137, 3172, 3413, 4039, 4249, 4274, 4360, 4547, 5280], 'on': [35, 233, 1013, 1748, 1772, 2162, 2208, 2226, 2288, 2371, 2579, 2782, 3127, 3702, 4253, 4361, 4935], 'conserved': [36, 234, 975, 2729, 3612, 4255, 4363, 5283], 'Asp-523.': [37, 235], 'required': [39, 194, 237, 392, 649, 921, 963, 1029, 2982, 3034, 3270, 4786, 4880, 5277, 5307], 'monovalent': [41, 239, 486, 1031, 3211], 'cation,': [42, 240, 1034], 'either': [43, 241, 923, 1035, 3158, 3272, 4180, 5147], 'Cu(I)': [44, 66, 154, 242, 264, 352, 490, 1036, 1056, 1153, 2692, 2970, 3105, 3161, 3198, 4082, 4114, 4181, 4375, 4433, 4531, 4557, 5244, 5311, 5320], 'or': [45, 51, 127, 164, 243, 249, 325, 362, 926, 1021, 1037, 1041, 1144, 2089, 2098, 2604, 2689, 2971, 3070, 3106, 3113, 3148, 3151, 3160, 3239, 3275, 3402, 3447, 3477, 3518, 3523, 3750, 3900, 4117, 4182, 4487, 4503, 4597, 4674, 4696, 4836, 4920, 5252], 'Ag(I).': [46, 244, 1038, 4183], 'Divalent': [47, 245, 1039], 'cations': [48, 246, 453, 487, 541, 697, 3146, 4406], 'Cu(II),': [49, 247, 2969, 3201, 4378, 4486], 'Zn(II),': [50, 248, 544], 'Co(II)': [52, 250, 548], 'could': [53, 251, 1043, 2850, 3099, 4430, 4447], 'not': [54, 68, 188, 252, 266, 386, 1044, 2810, 2901, 3152, 3183, 3200, 3213, 3556, 3597, 4041, 4377, 4381, 4444, 4477, 4533, 4543, 4604, 4849, 5006, 5223], 'substitute,': [55, 253], 'signifying': [56, 254], 'the': [58, 114, 124, 130, 138, 144, 180, 184, 189, 256, 312, 322, 328, 336, 342, 378, 382, 387, 428, 452, 483, 601, 679, 755, 787, 797, 803, 912, 917, 966, 984, 989, 999, 1062, 1067, 1073, 1082, 1088, 1093, 1121, 1222, 1241, 1250, 1255, 1323, 1327, 1330, 1341, 1396, 1454, 1493, 1527, 1556, 1565, 1568, 1578, 1758, 1803, 1806, 1819, 1844, 1853, 1912, 1995, 2003, 2008, 2019, 2103, 2142, 2172, 2186, 2200, 2203, 2221, 2274, 2300, 2315, 2390, 2458, 2500, 2509, 2521, 2543, 2572, 2713, 2723, 2728, 2733, 2737, 2742, 2816, 2842, 2862, 2866, 2869, 2877, 2883, 2889, 2895, 2929, 2953, 2957, 2984, 3043, 3048, 3051, 3062, 3109, 3118, 3141, 3144, 3164, 3175, 3187, 3235, 3246, 3257, 3262, 3321, 3353, 3361, 3372, 3387, 3392, 3425, 3486, 3515, 3547, 3551, 3560, 3563, 3585, 3593, 3617, 3652, 3700, 3719, 3747, 3758, 3772, 3777, 3811, 3843, 3850, 3872, 3881, 3895, 3934, 3937, 3947, 4001, 4009, 4018, 4024, 4053, 4101, 4104, 4134, 4167, 4208, 4212, 4217, 4243, 4248, 4259, 4362, 4389, 4393, 4455, 4480, 4496, 4506, 4522, 4553, 4564, 4569, 4593, 4622, 4647, 4650, 4655, 4659, 4666, 4688, 4699, 4702, 4731, 4753, 4767, 4782, 4802, 4806, 4809, 4825, 4833, 4838, 4859, 4868, 4875, 4885, 4907, 4923, 4927, 4965, 4969, 4979, 4985, 5000, 5003, 5013, 5115, 5131, 5134, 5159, 5171, 5182, 5207, 5216, 5236, 5262, 5282, 5301, 5313], 'substrate': [59, 257, 2958], 'of': [60, 85, 107, 116, 123, 129, 141, 169, 183, 258, 283, 305, 314, 321, 327, 339, 367, 381, 427, 430, 454, 479, 482, 678, 786, 911, 947, 1001, 1061, 1081, 1101, 1137, 1151, 1156, 1207, 1257, 1291, 1340, 1443, 1463, 1465, 1623, 1638, 1693, 1802, 1808, 1818, 1829, 1855, 1860, 1934, 1950, 1976, 2002, 2036, 2045, 2050, 2077, 2084, 2094, 2128, 2148, 2151, 2157, 2177, 2202, 2218, 2254, 2262, 2265, 2282, 2306, 2317, 2320, 2346, 2357, 2366, 2423, 2499, 2508, 2529, 2587, 2655, 2669, 2674, 2722, 2774, 2844, 2865, 2871, 2886, 2907, 2931, 2952, 2956, 2959, 2986, 3050, 3064, 3120, 3143, 3166, 3177, 3237, 3256, 3267, 3279, 3371, 3386, 3391, 3418, 3435, 3440, 3491, 3546, 3562, 3587, 3605, 3699, 3718, 3757, 3785, 3824, 3839, 3846, 3849, 3871, 3887, 3894, 3902, 3946, 3970, 3974, 4021, 4071, 4106, 4193, 4202, 4238, 4250, 4284, 4348, 4392, 4437, 4465, 4505, 4537, 4555, 4566, 4578, 4584, 4595, 4624, 4643, 4649, 4658, 4681, 4701, 4733, 4752, 4766, 4784, 4799, 4808, 4830, 4841, 4867, 4874, 4922, 4926, 4964, 4968, 4973, 4978, 4993, 5002, 5101, 5114, 5133, 5231, 5239, 5274, 5304, 5310], 'this': [61, 259, 881, 995, 1050, 3307, 3576, 4342, 4379, 4727, 4792], 'copper-translocating': [62, 260, 1051, 4280], 'Cu(II).': [69, 267, 1058, 3916, 4435], 'purified': [71, 269, 1130, 1138, 2169, 3960, 4025], 'from': [72, 270, 401, 589, 1240, 1405, 1843, 1852, 2027, 2739, 2772, 2804, 3229, 3408, 3438, 3730, 3792, 3921, 4090, 4616, 4684, 5174], 'dodecylmaltoside-solubilized': [73, 271], 'membranes': [74, 272, 1830, 4695], 'similarly': [75, 273, 4771], 'exhibited': [76, 274, 3883, 4027, 4141, 4186], 'Cu(I)/Ag(I)-stimulated': [77, 275], 'activity,': [79, 277, 4029], 'aK': [81, 279], 'm': [82, 280, 1701, 1704, 1924, 1982, 1985, 2059, 2086, 2284, 2308, 2526, 3821, 3838, 3870, 3886, 4190], 'for': [83, 153, 195, 281, 351, 393, 459, 650, 802, 922, 1076, 1229, 1266, 1326, 1567, 1649, 1738, 1798, 2110, 2138, 2168, 2171, 2210, 2228, 2242, 2290, 2454, 2469, 2556, 2707, 2841, 2983, 3035, 3097, 3271, 3400, 3806, 3822, 3842, 3915, 3936, 4191, 4211, 4216, 4278, 4374, 4571, 4582, 4787, 4811, 4814, 4881, 5243, 5266, 5278, 5308, 5319], 'ATP': [84, 282, 982, 2194, 2740, 2857, 4192], '0.5': [86, 284, 1932, 2252, 2260, 2283, 2530, 3831, 4194], 'mm.': [87, 285], 'has': [89, 287, 890, 1499, 2947, 3328, 3656, 4275, 4380, 4397, 5024, 5108, 5177], 'two': [90, 288, 898, 918, 1068, 1089, 3309, 3642, 4754, 4970], 'N-terminal': [91, 125, 190, 289, 323, 388, 690, 899, 1069, 1122, 3248, 3897, 3905, 4386, 4625, 4667, 4734, 4755, 4803, 4877], 'Cys(X)2Cys': [92, 290, 4769], 'sequences,': [93, 291, 901], 'Gly-Leu-Ser-Cys14-Gly-His-Cys17,': [94, 292], 'Gly-Met-Ser-Cys110-Ala-Ser-Cys113,': [96, 294], 'Cys479-Pro-Cys481': [99, 297], 'motif': [100, 133, 186, 298, 331, 384, 753, 1095, 1298, 2735, 3554, 3614, 3634, 3899, 4770, 5015, 5107, 5180, 5221, 5238], 'in': [101, 162, 167, 299, 360, 365, 585, 644, 698, 754, 794, 916, 1066, 1087, 1092, 1096, 1099, 1117, 1161, 1216, 1221, 1254, 1322, 1329, 1390, 1620, 1689, 1734, 1800, 1810, 1972, 2075, 2279, 2303, 2343, 2419, 2732, 2765, 2790, 2882, 3061, 3092, 3111, 3140, 3163, 3174, 3189, 3245, 3261, 3342, 3366, 3414, 3424, 3497, 3514, 3581, 3589, 3615, 3635, 3687, 3705, 3713, 3746, 3913, 3984, 4103, 4175, 4454, 4495, 4521, 4552, 4563, 4576, 4592, 4638, 4654, 4678, 4705, 4781, 4823, 4843, 4860, 4870, 4910, 4917, 4991, 5019, 5030, 5099, 5158, 5161, 5181, 5312], 'membrane-spanning': [102, 300], 'segment': [103, 301, 758], 'six.': [104, 302], 'The': [105, 303, 396, 1010, 1135, 1289, 1374, 1481, 1534, 1602, 1684, 1944, 1968, 2015, 2131, 2245, 2332, 2446, 2472, 2491, 2516, 2552, 2635, 2699, 2823, 2848, 3095, 3632, 3641, 3697, 3790, 3801, 3818, 3867, 3950, 3968, 4067, 4138, 4198, 4235, 4524, 4609, 4640, 4846, 5105], 'requirement': [106, 304, 3096, 4570, 5132], 'these': [108, 306, 1110, 2966, 3409, 4428, 4644, 5232], 'cysteine': [109, 181, 191, 307, 379, 389, 914, 1064, 1090, 3071, 3259, 4519, 4586, 4634, 4652, 4735, 4750, 4966, 5135], 'residues': [110, 182, 308, 380, 915, 1065, 1091, 1450, 1510, 3260, 4257, 4653, 4751, 4967, 5303], 'investigated': [112, 310, 998, 3766, 4730], 'effect': [115, 313, 3119, 3698, 4934], 'mutations': [117, 315, 1060, 1321, 1551, 3701, 4847], 'deletions.': [119, 317], 'Mutants': [120, 318], 'substitutions': [122, 161, 320, 359, 3513], 'cysteines': [126, 324, 967, 1111, 1328, 3110, 3527, 3643, 3906, 4878], 'deletion': [128, 326, 946, 1080, 1293, 1442, 1501, 2806, 3278, 3355, 3545, 4765, 4921, 4977], 'first': [131, 329, 1083, 1455, 3322, 3516, 3552, 3896, 4768, 4924, 4986], 'Cys-(X)2-Cys': [132, 330, 691, 804, 900, 1070, 1084, 1297, 1456, 1515, 3241, 3323, 3330, 3521, 3553, 3898, 4390, 4703, 4756, 4826, 4908, 4929, 4971], 'intermediates.': [136, 334], 'From': [137, 335], 'dependence': [140, 338, 3845], 'phosphoenzyme': [142, 174, 340, 372, 1106, 2763, 2898, 3036, 3136, 3171, 3742, 4038, 4546, 4789], 'formation,': [143, 341, 1107, 3037], 'mutants': [145, 343, 1299, 3510, 4736, 5248], 'appear': [146, 344, 3557], 'have': [148, 346, 906, 973, 3251, 4448, 4514, 4723, 4903], '2–3': [149, 347], 'fold': [150, 348, 5007], 'higher': [151, 349, 4132], 'affinity': [152, 350, 1075, 2481, 3914, 3964, 4807], 'than': [155, 353, 1120, 3081, 4133, 4581], 'wild': [156, 354, 1603, 1820, 2779, 3373, 3451, 3536, 3691, 3825], 'type': [157, 355, 1604, 1821, 2780, 3374, 3452, 3537, 3692, 3770, 3826], 'CopA.': [158, 356, 3989, 4022, 4349], 'In': [159, 357, 639, 987, 994, 1559, 2868, 2928, 3045, 3156, 4123, 4341, 4489, 4664, 4712, 4726, 4774, 5009], 'contrast,': [160, 358, 3157], 'Cys479': [163, 361, 3638, 5137], 'Cys481': [165, 363, 5139], 'resulted': [166, 364, 1098, 4990], 'loss': [168, 366, 1100, 3586, 4992], 'resistance,': [171, 369, 1103, 4853], 'transport': [172, 370, 927, 2968, 2985, 3052, 3288, 3712], 'formation.': [175, 373, 1026, 4790], 'These': [176, 374, 3204, 3378, 4163], 'results': [177, 375, 3049, 3205, 3379, 4164, 4862, 5233], 'imply': [178, 376, 4796], 'Cys-Pro-Cys': [185, 383, 752, 1094, 1331, 3613, 3633, 5014, 5106, 5237, 5302], '(but': [187, 385, 3212], 'residues)': [192, 390], 'are': [193, 391, 583, 598, 686, 968, 1576, 2835, 4372, 4614, 4661, 4716, 4879, 4975, 5306], 'function.': [197, 395], '834-residue': [397], 'pump': [400, 4431, 4810, 4869], 'coli(1Rensing': [403], 'C.': [404, 563, 605, 656, 933, 1308, 1418, 1582, 1664, 1889, 2992, 3017, 3294, 3624, 5121], 'Fan': [405, 657, 1419, 1583, 1665, 1890, 2993], 'B.': [406, 410, 468, 565, 607, 658, 662, 720, 744, 929, 1304, 1420, 1424, 1584, 1588, 1666, 1670, 1891, 1895, 2994, 2998, 3013, 3290, 4416], 'Sharma': [407, 659, 1421, 1585, 1667, 1892, 2995], 'R.': [408, 526, 660, 1422, 1586, 1668, 1893, 2629, 2996, 3665, 4315, 5190], 'Mitra': [409, 467, 564, 661, 1423, 1587, 1669, 1894, 2997], 'Rosen': [411, 469, 566, 663, 934, 1309, 1425, 1589, 1671, 1787, 1896, 2999, 3018, 3295, 5081], 'B.P.': [412, 470, 567, 664, 935, 1310, 1426, 1590, 1672, 1788, 1897, 3000, 3019, 3296, 5082], 'Proc.': [413, 568, 665, 1427, 1591, 1673, 1898, 3001], 'Natl.': [414, 569, 666, 1428, 1592, 1674, 1899, 3002], 'Acad.': [415, 570, 667, 1429, 1593, 1675, 1900, 3003], 'Sci.': [416, 571, 668, 1430, 1594, 1676, 1901, 2756, 3004, 3627, 4268, 5124], 'U.': [417, 572, 669, 1431, 1595, 1677, 1902, 3005], 'S.': [418, 514, 573, 609, 611, 670, 701, 739, 828, 855, 1432, 1596, 1678, 1782, 1903, 3006, 4321, 4410, 4946, 5043], 'A.': [419, 508, 522, 574, 671, 1433, 1597, 1679, 1904, 3007, 3661, 5035, 5037, 5057, 5186], '2000;': [420, 474, 672, 746, 1434, 1598, 1680, 1905, 3008], '97:': [421, 673, 1435, 1599, 1681, 1906, 3009], '652-656Google': [422, 674, 1436, 1600, 1682, 1907, 3010], 'Scholar)': [423, 619, 637, 1181], 'member': [426], 'superfamily': [429, 4244], 'cation-transporting': [431], 'ATPases': [433, 582, 685, 972, 2725, 2967, 3228, 3608, 4240, 4660, 5023], '(2Axelsen': [434], 'K.B.': [435], 'Palmgren': [436], 'M.G.': [437], 'J.': [438, 471, 494, 529, 555, 613, 710, 718, 817, 819, 830, 837, 859, 860, 873, 1165, 1272, 2380, 2381, 3668, 3860, 4228, 4292, 4302, 4306, 4307, 4322, 4417, 4948, 4955, 5064, 5083, 5193, 5290], 'Mol.': [439], 'Evol.': [440], '1998;': [441, 730], '46:': [442], '84-101Google': [443], 'Scholar).': [444, 477, 535, 578, 675, 943, 1288, 1318, 1437, 1601, 1683, 1795, 1908, 2387, 2634, 2760, 3027, 3304, 3631, 3866, 4234, 4272, 4340, 4961, 5128, 5296], 'belongs': [446], 'subfamily': [449, 484], 'transports': [451, 485, 539], 'soft': [455, 462, 680, 695, 1032, 3124, 3216, 3225, 3606, 4610, 5020], 'Lewis': [456], 'acids': [457], '(or,': [458], 'simplicity,': [460], 'just': [461, 3571, 4983], 'metal': [463, 681, 696, 1033, 1132, 1210, 2095, 3125, 3217, 3226, 3232, 3242, 3607, 3962, 4611, 4619, 4626, 4672, 5021, 5268, 5275], 'cations)': [464], '(3Gatti': [465], 'D.': [466, 722, 825, 847, 1784, 4304, 4943, 5041], 'Biol.': [472, 530, 556, 711, 729, 838, 861, 874, 3669, 3861, 4229, 4293, 4308, 4323, 4418, 4956, 5065, 5194], 'Chem.': [473, 531, 557, 712, 839, 862, 875, 3670, 3862, 4230, 4294, 4309, 4324, 4419, 4957, 5066, 5195], '275:': [475], '34009-34012Google': [476], 'Members': [478], 'one': [480, 687, 3238], 'branch': [481, 538], 'such': [488, 542, 2541], 'as': [489, 543, 1078, 1202, 1531, 1660, 1778, 1885, 3090, 3350, 3352, 3422, 3572, 5103], 'Ag(I)': [492, 3159, 4559], '(4Camakaris': [493], 'Voskoboinik': [495], 'I.': [496, 845, 4300], 'Mercer': [497, 808, 835, 856, 4953], 'J.F.': [498, 512, 809, 836, 857, 4317, 4954], 'Biochem.': [499, 936, 1311, 1790, 2755, 3020, 3297, 3626, 4267, 4333, 5123], 'Biophys.': [500, 937, 1312, 1791, 3021, 3298, 4334], 'Res.': [501, 938, 1313, 3022, 3299, 4335, 5046], 'Commun.': [502, 939, 1314, 3023, 3300, 4336], '1999;': [503, 517, 558, 840, 863, 876, 4958, 5048], '261:': [504], '225-232Google': [505], 'Scholar,': [506, 520, 561, 716, 733, 823, 843, 866, 3011, 4298, 4313, 4328, 5051, 5070], '5Gupta': [507], 'Matsui': [509], 'K.': [510, 703, 5033, 5053], 'Lo': [511], 'Silver': [513], 'Nat.': [515, 614, 632, 727], 'Med.': [516], '5:': [518, 635, 731], '183-188Google': [519], '6Odermatt': [521], 'Suter': [523, 3662, 5187], 'H.': [524, 851, 3663, 4330, 5188], 'Krapf': [525, 3664, 5189], 'Solioz': [527, 3666, 4331, 5191], 'M.': [528, 735, 849, 853, 3622, 3667, 4332, 4408, 5045, 5119, 5192], '1993;': [532, 616, 634, 3671, 5196], '268:': [533, 3672, 5197], '12775-12779Google': [534, 3673, 5198], 'A': [536, 1438, 2719, 3602, 3990, 4744, 5228], 'second': [537, 3519, 4633], 'divalent': [540, 3123, 3145, 4405, 4599, 4606], 'Cd(II),': [545], 'Pb(II),': [546], '(7Rutherford': [549], 'J.C.': [550], 'Cavet': [551], 'J.S.': [552], 'Robinson': [553], 'N.J.': [554], '274:': [559, 841, 864, 877, 4959], '25827-25832Google': [560], '8Rensing': [562], '1997;': [575, 713, 4420], '94:': [576], '14326-14331Google': [577], 'Copper': [579], 'translocating': [580, 683], 'found': [584, 3981, 4461, 5157], 'virtually': [586], 'every': [587], 'organism,': [588], 'E.': [590, 640, 887, 894, 1157, 2657, 2753, 4265, 4440, 4713, 5175], 'humans': [593], 'include': [595], 'homologues': [596, 3233, 4283, 4620, 5012], 'related': [599], 'human': [602], 'Menkes': [603, 775], '(9Vulpe': [604], 'Levinson': [606], 'Whitney': [608], 'Packman': [610], 'Gitschier': [612], 'Genetics.': [615, 633], '3:': [617], '7-13Google': [618], 'Wilson': [621, 779, 4394], '(10Bull': [622], 'P.C.': [623], 'Thomas': [624], 'G.R.': [625], 'Rommens': [626], 'J.M.': [627, 3859, 4227, 4291], 'Forbes': [628, 4411], 'J.R.': [629, 868, 4412], 'Cox': [630, 871, 4413], 'D.W.': [631, 872, 4414], '327-337Google': [636], 'diseases.': [638], 'participates': [643], 'homeostasis': [646], 'efflux': [652], 'resistance': [654, 925, 954, 3274, 3286, 3339, 3580, 3588, 3704, 3723], '(1Rensing': [655, 1417, 1581, 1663, 1888, 2991], 'Distinctive': [676], 'features': [677], 'ion': [682, 2096, 2915, 2961, 3218, 5314], 'six': [689, 1572, 3941, 4928], 'sequences': [692, 805, 919, 1071, 3266], 'bind': [694, 4401, 5256], 'vitro': [699, 3714, 4824, 4861], '(11Lutsenko': [700], 'Petrukhin': [702], 'Cooper': [704], 'M.J.': [705, 807], 'Gilliam': [706], 'C.T.': [707], 'Kaplan': [708, 4318], 'J.H.': [709, 4319], '272:': [714, 4421], '18939-18944Google': [715], '12Gitschier': [717], 'Moffat': [719], 'Reilly': [721], 'Wood': [723], 'W.I.': [724], 'Fairbrother': [725], 'W.J.': [726], 'Struct.': [728, 2383], '47-54Google': [732], '13DiDonato': [734], 'Hsu': [736], 'H.F.': [737], 'Narindrasorasak': [738, 4409], 'Que': [740], 'Jr.,': [741], 'L.': [742], 'Sarkar': [743, 4415], 'Biochemistry.': [745, 2630], '39:': [747], '1890-1896Google': [748], 'Scholar),': [749, 879, 3674, 4423, 5089, 5199], 'sixth': [756], 'transmembrane': [757, 765, 4988], '(TM)': [759], '1The': [760], 'abbreviations': [761], 'used': [762, 1265, 2074], 'are:': [763], 'TM,': [764], 'segment;': [766], 'DTT,': [767, 2087, 2980, 3068, 3178, 4556], 'dithiothreitol;': [768], 'BCDS,': [769], 'bathocuproindisulfonate;': [770], 'DDM,': [771, 2652], 'dodecyl': [772, 2443], 'maltoside,': [773], 'MNK,': [774], 'disease-related': [776, 780], 'protein;': [777], 'WND,': [778], 'protein': [781, 2247, 2517, 3393, 3437, 4004, 4026, 4396, 4585, 5004, 5217], 'may': [783, 4085, 4669, 4675, 4893], 'be': [784, 2838, 2851, 2920, 2923, 3100, 3406, 4575, 4676, 4894, 5028, 5112], 'part': [785, 5113], 'translocation': [788, 3619, 4834, 5116, 5315], 'pathway.': [789], 'Thus': [790, 4568], 'far': [791], 'intracellular': [792, 892, 4685, 4694, 4718], 'trafficking': [793, 4680], 'eukaryotes': [795, 4665], 'only': [798, 3060, 4896, 4939], 'physiological': [799, 4641, 4899], 'role': [800, 1116, 4642], 'identified': [801, 4940], '(14Petris': [806], 'Culvenor': [810], 'J.G.': [811], 'Lockhart': [812, 833, 4951], 'P.': [813], 'Gleeson': [814], 'P.A.': [815], 'Camakaris': [816, 858, 4305], 'EMBO': [818], '1996;': [820, 3628, 5067, 5125], '15:': [821, 2632], '6084-6095Google': [822], '15Strausak': [824], 'La': [826, 4944], 'Fontaine': [827, 4945], 'Hill': [829, 4947], 'Firth': [831, 4949], 'S.D.': [832, 4950], 'P.J.': [834, 4952], '11170-11177Google': [842, 4960], '16Voskoboinik': [844], 'Strausak': [846, 4303], 'Greenough': [848], 'Brooks': [850], 'Petris': [852], 'Smith': [854], '22008-22012Google': [865], '17Forbes': [867], 'Hsi': [869], 'G.': [870, 931, 1306, 2378, 2627, 3015, 3292, 5061, 5287], '12408-12413Google': [878], 'but': [880, 4474, 4510, 4995, 5164], 'unlikely': [883], 'apply': [885], 'coli,': [888], 'which': [889, 1391, 1498, 1575, 1866, 2834, 2875, 3196, 3316, 3327, 3833, 3927, 4499], 'no': [891, 2934, 2949, 4590, 4717, 4721, 4932, 5254], 'membranes.': [893], 'contains': [897], 'Gly-Leu-Ser-Cys14-Gly-His-Cys17': [902, 3263], 'Gly-Met-Ser-Cys110-Ala-Ser-Cys113.': [904], 'We': [905, 3250], 'previously': [907, 1302, 1662, 1780, 1887, 3252], 'shown': [908, 3253, 4277, 4399, 5026], 'none': [910, 3255, 4873, 4963], 'four': [913, 1063, 1834, 3258, 3526, 3904, 4749, 4876, 4925], '(18Fan': [928, 1303, 3289], 'Grass': [930, 1305, 3014, 3291], 'Rensing': [932, 1307, 3016, 3293], '2001;': [940, 1315, 3024, 3301, 4310, 4337], '286:': [941, 1316, 3025, 3302], '414-418Google': [942, 1317, 3026, 3303], 'However,': [944], 'codons': [948, 1444, 1503, 3280, 3943], '8–150': [949, 3281], '(ΔNCopA)': [950, 3282], 'lost': [951, 3283], 'both': [952, 1514, 3284, 3329, 4432, 4490, 4775], 'transport,': [956, 1104, 3276], 'suggesting': [957], 'an': [959, 1635, 1763, 2046, 2229, 2324, 2354, 2372, 2845, 2905, 3531, 3884, 3910, 4110, 4187, 4251, 4385, 4864, 5225], 'N': [960, 3389, 3548, 4019, 4980], 'terminus': [961, 3390, 3549, 4020, 4981], 'even': [964, 3173, 3359], 'though': [965, 3360], 'not.': [969], 'All': [970, 1550, 2598, 2610, 3681], 'aspartate': [976, 2730, 4256, 5284], 'residue': [977, 991, 2731, 5285], 'during': [983, 2741, 3976, 4258], 'catalytic': [985, 2743, 4260, 5264], 'cycle.': [986], 'corresponding': [990, 1335, 2817, 5183], 'Asp523.': [993, 4364], 'report': [996, 4343, 4728], 'we': [997, 4344, 4729, 5200, 5298], 'ability': [1000, 3561, 4732], 'form': [1004, 3165, 3567, 3598, 4740, 5224], '32P': [1011], 'label': [1012, 2849], 'treatment': [1018], 'alkali': [1020, 2272, 4354], 'Phosphoenzyme': [1027, 4273], 'Cu(II)': [1040, 3065, 3103, 4093, 4118, 4402, 4507, 4534, 4551, 4596], 'Zn(II)': [1042, 3147, 4095], 'substitute.': [1045], 'This': [1046, 4446, 5258], 'clearly': [1047, 4528], 'demonstrates': [1048], 'distinguishes': [1054], 'between': [1055], 'Paradoxically,': [1059], 'increased': [1072, 4865], 'apparent': [1074, 3819, 3868, 3911, 4188, 4895, 4933], 'copper,': [1077, 3725, 4812, 5214], 'did': [1079, 2809, 3555, 3596, 4848, 5222], 'sequence.': [1085], 'Mutations': [1086], 'TM6': [1097, 5031, 5100, 5184], 'indicating': [1108, 2861], 'play': [1112], 'more': [1114, 3079, 3240, 3985], 'critical': [1115], 'function': [1119, 4648, 4700, 4892, 4941], 'cysteines.': [1123], 'solubilized': [1126, 2418, 3953], 'dodecylmaltoside': [1128], 'chelate': [1133, 3963], 'chromatography.': [1134], 'activity': [1136, 2618, 3401, 3848, 4056, 4070, 4127, 4140, 4150, 4170, 4481, 4883], 'stimulated': [1141, 2924, 3170, 4096, 4483, 4549], 'silver': [1145], 'ions': [1146, 3126], 'addition': [1150, 1949, 2147, 2217, 2316], 'chelator.': [1154], 'Cells': [1155, 1613, 1643, 3333, 5145, 5205], 'were': [1159, 1200, 1213, 1264, 1300, 1376, 1552, 1608, 1614, 1644, 1658, 1687, 1719, 1730, 1880, 1957, 1970, 1997, 2025, 2040, 2073, 2100, 2224, 2335, 2369, 2392, 2417, 2532, 2554, 2564, 2600, 2612, 2697, 2770, 3040, 3078, 3313, 3349, 3364, 3644, 3685, 3715, 3735, 3744, 3797, 3803, 3808, 3919, 3952, 4443, 4460, 4560, 5140, 5156, 5210], 'grown': [1160, 1214, 1615], 'Luria-Bertani': [1162, 1217], 'medium': [1163, 1224, 1629], '(19Sambrook': [1164, 1271], 'Fritsch': [1166, 1273], 'E.F.': [1167, 1274], 'Maniatis': [1168, 1275], 'T.': [1169, 1276, 5039, 5055], 'Molecular': [1170, 1277], 'cloning,': [1171, 1278], 'laboratory': [1173, 1280], 'manual.': [1174, 1281], 'Cold': [1175, 1282], 'Spring': [1176, 1283], 'Harbor': [1177, 1284], 'Laboratory,': [1178, 1285], 'New': [1179, 1286], 'York.1989Google': [1180, 1287], 'at': [1182, 1232, 1243, 1577, 1617, 1640, 1652, 1712, 1741, 1775, 1833, 1882, 2107, 2135, 2153, 2165, 2238, 2329, 2451, 2465, 2614, 2704, 2815, 3382, 3813, 4017, 4151, 4160, 5281], '37': [1183, 1233, 1618, 2705], '°C.': [1184, 1654, 2616], 'Ampicillin': [1185], '(100': [1186], 'μg/ml),': [1187, 1190], 'chloramphenicol': [1188], '(30': [1189], 'isopropyl-β-d-thiogalactopyranoside': [1191], '(0.1': [1192], 'mm),': [1193], '5-bromo-4-chloro-3-indolyl-β-d-galactosidase': [1194], '(80': [1195], 'μg/ml)': [1196], 'l(+)-arabinose': [1198], '(0.0002%)': [1199], 'added': [1201, 2123, 2183, 2196, 3041, 3089], 'required.': [1203, 4544], 'To': [1204, 1319, 1796, 2113, 2270, 2761, 2944, 3028, 3305, 5129], 'assay': [1205, 1766, 2020, 2047, 2625, 2714], 'inhibition': [1206], 'growth': [1208], 'salts,': [1211], 'cells': [1212, 2773, 3345, 3439, 3590, 3731, 3923], 'overnight': [1215, 1616], 'medium,': [1218], 'diluted': [1219, 1625, 2041, 2352], '1:100': [1220], 'same': [1223, 1251, 1528, 2004, 2501, 2510, 2522], 'CuSO4,': [1226], 'incubated': [1228, 2106, 2134, 2703], '6': [1230, 3998], 'h': [1231, 1651], '°C': [1234, 1619, 1714, 2137, 2453, 2706], 'shaking.': [1236], 'Growth': [1237], 'monitored': [1239], 'absorbance': [1242], '600': [1244, 1641], 'nm.': [1245, 1642], 'Each': [1246, 1827, 3430], 'strain': [1247, 1267, 1294, 1611, 2775, 2807, 3356, 3574], 'achieved': [1248], 'approximately': [1249, 4148], 'final': [1252, 2126, 2155, 2544], 'density': [1253, 1637], 'absence': [1256, 2930, 3176, 4565], 'copper.': [1258, 5152], 'Standard': [1259], 'molecular': [1260], 'genetic': [1262], 'techniques': [1263], 'plasmid': [1269, 1347, 1388, 1407, 1478, 1494, 1545, 1548, 3444, 3925], 'construction': [1270], 'construct': [1290], 'copA': [1292, 1342, 1392, 1439, 1464, 1561, 2781, 3930], 'DC194': [1295, 2776, 2808, 3441], 'described': [1301, 1661, 1779, 1886, 3423], 'introduce': [1320], 'coding': [1324], 'sequence': [1325, 1334, 1566, 3659, 3935], 'motif,': [1332, 3324, 4635], 'base': [1337], 'pairs': [1338], '892–1854': [1339], 'gene': [1343, 1402, 1440, 3931], 'cloned': [1345], 'into': [1346, 1387, 1395, 1415, 1477, 1490, 1544, 1610, 1627, 2042, 2534], 'pGEM-T': [1348, 1479], 'Easy': [1349], '(Promega)': [1350], 'polymerase': [1352], 'chain': [1353], 'reaction': [1354, 1913, 1945, 2143, 2636, 2700, 2890, 4177], '(PCR)': [1355], 'using': [1356, 1369, 1466, 1521, 1721, 1762, 2398, 2567], 'forward': [1357, 1467, 1522], 'primer': [1358, 1362, 1468, 1472, 1523, 1530], '5′-CTCGGCCATATGCTGGAAGCG-3′': [1359], 'reverse': [1361, 1471, 1529], '5′-GTAATCGCCGCGGTTTCAATG-3′.': [1363], 'Site-directed': [1364], 'mutagenesis': [1365], 'carried': [1367], 'out': [1368], 'QuickChange': [1371], 'method': [1372, 1725], '(Stratagene).': [1373], 'inserts': [1375], 'excised': [1377, 1404, 1484, 1538], 'double': [1379, 1486, 1540], 'digestion': [1380, 1409, 1487, 1541], 'NdeI': [1382], 'SacII': [1384, 1412], 'ligated': [1386, 1414], 'pGEXCopA2': [1389], 'inserted': [1394], 'pGEX-6p-2': [1397], 'vector': [1398], '(Amersham': [1399], 'Biosciences).': [1400], 'ThecopA': [1401], 'withNcoI': [1410], 'pCopA2': [1416, 1546, 1560], '7–54': [1445], '(corresponding': [1446, 1506], 'amino': [1448, 1508], 'acid': [1449, 1509, 1724, 2181, 2340], '3–18': [1451], 'including': [1453], 'sequence)': [1457], 'generated': [1459, 1518], 'PCR': [1461, 1520, 1535], 'amplification': [1462], '5′-GTTCCATGGCAAAACGCGTGAAAGAAAG-3′': [1469], '5′-ATGGCCGCCGGCGAAAACCATCACTGC-3′,': [1473], 'subsequent': [1475, 3093], 'cloning': [1476], 'Easy.': [1480], 'insertion': [1482], 'byNcoI/NaeI': [1485, 1539], 'ligation': [1489, 1543], 'pCopA2,': [1491, 3926], 'generating': [1492, 1547], 'pCopA2ΔN1.': [1495, 1533], 'Plasmid': [1496], 'pCopA2ΔN2,': [1497, 3326, 3592], 'ofcopA': [1502, 1580], '7–342': [1504], 'bp': [1505], '3–114': [1511], 'includes': [1513, 3637], 'sequences)': [1516], '5′-': [1524], 'GTTCCAATGGCAACCCGCGTACAAAATGCG-3′': [1525], 'product': [1536], 'pCopA2ΔN2.': [1549], 'verified': [1553], 'sequencing': [1555], 'entire': [1557], 'insert.': [1558], 'in-frame': [1563, 3932], 'Myc': [1569, 3938], 'epitope': [1570, 3939], 'histidine': [1573, 1920, 2053, 3942, 4010], 'codons,': [1574], '3′-end': [1579], 'mutant': [1606, 1804, 3577, 3677, 3683, 3720, 3779, 3874, 4746, 5148, 5154, 5208], 'pCopA2plasmid': [1607], 'introduced': [1609], 'DC194.': [1612], '5': [1621, 1941, 1951, 2000, 2062, 2071, 2111, 2118, 2139, 2291, 2435, 2496, 2505, 2677, 2708, 2716, 3788], 'ml': [1622, 2001, 2253, 2261, 2281, 2305, 2319, 2531, 2586], 'LB,': [1624], '100-fold': [1626], 'prewarmed': [1628], 'allowed': [1631], 'grow': [1633], 'optical': [1636], '0.7': [1639], 'induced': [1645], '0.0002%': [1647], 'arabinose': [1648, 3948], '2.5': [1650, 2149], '30': [1653, 1739, 2197, 2214, 2344, 2798], 'Everted': [1655, 2031], 'membrane': [1656, 1685, 1812, 1935, 2023, 2032, 2222, 2768, 2989, 3436, 3728, 5160], 'vesicles': [1657, 1686, 1813, 2024, 2033, 2223, 2414, 2769, 2990, 3729, 3918, 3951], 'prepared': [1659, 1731, 2771, 3920], 'suspended': [1688, 2278], 'buffer': [1691, 1737, 1974, 2048, 2351, 2420, 2486, 2511, 2523, 2593], 'consisting': [1692, 1975, 2049, 2422], '25': [1694, 2424], 'mm': [1695, 1708, 1919, 1929, 1942, 1988, 2052, 2119, 2192, 2425, 2430, 2436, 2514, 2642, 2647, 2663, 2678, 2685, 2717, 2855, 4195], 'Tris-HCl,': [1696, 2426], 'pH': [1697, 1979, 2268, 2311, 2360, 2427, 2644, 2829, 4145, 4152, 4161], '7.0,': [1698, 2428], 'containing': [1699, 2066, 2362, 2441, 2488, 2512, 2524, 2536, 2595, 4388], '0.25': [1700, 1984, 2058, 2662, 2684], 'sucrose,': [1702, 1927, 1986, 2060], '0.2': [1703, 1923, 1981, 2056, 2280, 2304, 2318, 3890], 'KCl,': [1705, 1925, 1983, 2431, 2648], '0.05': [1707], 'EDTA': [1709, 2540], 'stored': [1711], '−70': [1713], 'until': [1715], 'use.': [1716, 2609], 'Protein': [1717], 'concentrations': [1718, 2545, 3784], 'determined': [1720], 'bicinchoninic': [1723], '(Sigma': [1726], 'Chemical': [1727], 'Co.).': [1728], 'Samples': [1729, 2365], 'incubation': [1733, 2161, 2207, 2328], 'SDS': [1735, 2349], 'sample': [1736, 2350], 'min': [1740, 2232, 2327, 2709], 'room': [1742, 1776, 1883, 2108, 2166, 2330], 'temperature': [1743, 1884, 2109, 2167], 'separated': [1745], 'SDS-PAGE': [1747, 2559], '8%': [1749, 2374], 'polyacrylamide': [1750, 2375], 'gels.': [1751], 'Immunoblotting': [1752], 'performed': [1754, 1881, 2613], 'antibody': [1756, 3484, 4007], 'His6': [1759, 3487], 'tag': [1760], '(Clontech)': [1761], 'enhanced': [1764], 'chemiluminescence': [1765], '(PerkinElmer': [1767], 'Life': [1768], 'Sciences)': [1769], 'exposed': [1771], 'x-ray': [1773], 'film': [1774], 'temperature,': [1777], '(20Dey': [1781], 'Dou': [1783], 'Tisa': [1785], 'L.S.': [1786], 'Arch.': [1789], '1994;': [1792], '311:': [1793], '418-424Google': [1794], 'correct': [1797], 'differences': [1799], 'expression': [1801], 'proteins,': [1805], 'amount': [1807, 3973], 'everted': [1811, 2767, 3727], 'normalized': [1815, 3809], 'quantitative': [1823], 'Western': [1824], 'blot': [1825], 'analysis.': [1826], 'set': [1828], 'analyzed': [1832, 2407, 2555], 'different': [1835, 4898], 'concentrations,': [1836], 'standard': [1839], 'curve': [1840], 'constructed': [1842, 5201], 'values': [1845, 2016, 3802], 'obtained': [1846, 1851, 2017], 'densitometry.': [1848, 3800], '64Cu': [1849, 2987], 'Division': [1854], 'Radiological': [1856], 'Science,': [1857], 'Mallinckrodt': [1858], 'Institute': [1859], 'Radiology,': [1861], 'Washington': [1862], 'University': [1863], 'Medical': [1864], 'School,': [1865], 'supported': [1868], 'NCI': [1870], 'Research': [1871], 'Resource': [1872], 'Grant': [1873], 'NIH': [1874], '1': [1875, 1928, 2037, 2191, 2432, 2455, 2470, 2547, 2682, 2801, 2854, 2940, 3073, 3133, 4047], 'R24': [1876], 'CA86307.': [1877], 'Transport': [1878], 'assays': [1879, 2065], 'Unless': [1909], 'otherwise': [1910], 'noted,': [1911], 'mixture': [1914, 2021, 2104, 2132, 2447, 2637, 2701], '(1': [1915], 'ml)': [1916, 2639], 'contained': [1917, 2640, 3432], '40': [1918, 1977, 2051, 2175, 2513, 2641], '(pH': [1921, 2054], '6.8),': [1922, 2055], '0.25m': [1926, 2321], 'dithiothreitol': [1930], '(DTT),': [1931], 'mg': [1933, 2654], 'protein,': [1936], '10': [1937, 1987, 2231, 2243, 2326, 2367, 2672, 2687, 2690], 'μm64CuCl2(0.5–10': [1938], 'μCi/ml),': [1939], 'Na2ATP.': [1943], 'initiated': [1947, 2145], 'mmMgCl2.': [1952, 2063], 'At': [1953], 'intervals,': [1954], '0.1-ml': [1955], 'samples': [1956, 2334, 2553], 'withdrawn': [1958], 'filtered': [1960], 'through': [1961, 3550], 'nitrocellulose': [1962], 'filters': [1963, 1969, 1996], '(0.22-μm': [1964], 'pore': [1965], 'size,': [1966], 'Whatman).': [1967], 'presoaked': [1971], 'mmhistidine,': [1978], '6.8,': [1980, 2645], 'MgSO4,': [1989], '20': [1991, 3433], 'mmCuCl2.': [1992], 'Following': [1993, 2216, 2388], 'filtration,': [1994], 'washed': [1998, 2249, 2494], 'buffer,': [2005, 2502], 'dried,': [2006], 'radioactivity': [2009, 3496], 'quantified': [2010, 3798], 'liquid': [2012], 'scintillation': [2013], 'counting.': [2014], 'without': [2022, 3442], 'subtracted': [2026], 'all': [2028, 3525, 3903, 4504, 4748], 'time': [2029], 'points.': [2030], '(20': [2034], 'μl': [2035, 2044, 2083, 2093, 2176, 2345, 2368], 'mg/ml': [2038], 'protein)': [2039], '140': [2043], 'mKCl,': [2057], 'For': [2064, 2188, 3083, 3115, 4915], 'AgNO3,': [2067], '0.2m': [2068], 'KNO3,': [2069], 'mmMg(NO3)2': [2072], 'place': [2076], 'KCl': [2078], 'MgCl2.': [2080, 2718], 'Reductant': [2081], '(1.5': [2082], '0.1': [2085, 2307, 2525, 2549, 3878], 'GSH,': [2088, 3069], 'cysteine)': [2090], '1.5': [2092, 3829], 'solutions': [2097], 'water': [2099, 2256], 'added,': [2101], 'min.': [2112, 2244, 2292], 'measure': [2114], 'sensitivity': [2115], 'vanadate,': [2117], 'sodium': [2120, 2309, 4035, 4044], 'orthovanadate': [2121], 'concentration': [2127, 2156, 3764, 4783, 4840], '50': [2129, 2158, 2266, 2358, 2429, 2646, 2872, 3814], 'μm.': [2130, 2159, 3891], '4': [2136, 2452, 2615, 3708], 'min,': [2140], 'μCi': [2150], '[γ-32P]ATP': [2152, 2789], 'After': [2160], 'ice': [2163, 2209, 2227, 2289], '(or': [2164], 'CopA)': [2170], 'indicated': [2173, 2681], 'times,': [2174], 'ice-cold': [2178], '50%': [2179], 'tricholoracetic': [2180, 2219, 2339], 'terminate': [2185], 'reaction.': [2187], 'pulse-chase': [2189], 'analysis,': [2190], 'cold': [2193], 's': [2198, 2799], 'after': [2199, 3320], 'start': [2201], 'reaction,': [2204], 'followed': [2205, 2313, 2503], 'another': [2211], '15': [2212, 2859], 's.': [2215], 'acid,': [2220], 'kept': [2225, 2287], 'additional': [2230, 2325], 'then': [2234], 'harvested': [2235], 'centrifugation': [2237, 2464], '15,000': [2239], '×': [2240, 2467], 'g': [2241, 2468], 'pellet': [2246], 'once': [2250, 2258], 'distilled': [2255], 'solution': [2264], 'mmH3PO4/NaOH,': [2267, 2359], '2.4.': [2269], 'examine': [2271, 5130], 'lability,': [2273], 'pelleted': [2275, 2301], 'material': [2276, 2302], 'Sensitivity': [2293], 'hydroxylamine': [2295, 2831], 'examined': [2297, 3131], 'suspending': [2299], 'acetate,': [2310], '5.6,': [2312], 'NH2OH,': [2322], 'temperature.': [2331], 'treated': [2333], 'again': [2336], 'precipitated': [2337], 'dissolved': [2342], '2×': [2347], 'concentrated': [2348, 2537, 2565], 'equal': [2355, 4149], 'volume': [2356], '2.4,': [2361], '5%': [2363], 'SDS.': [2364], 'loaded': [2370, 2476], 'acidic': [2373], 'gel': [2376, 2577], '(21Fairbanks': [2377], 'Avruch': [2379], 'Supramol.': [2382], '1972;': [2384], '1:': [2385], '66-75Google': [2386], 'electrophoresis,': [2389], 'gels': [2391, 3796], 'stained': [2393], 'Coomassie': [2395], 'Blue,': [2396], 'dried': [2397], 'DryEase': [2400], 'mini-gel': [2401], 'drying': [2402], 'system': [2403], '(Novex).': [2404], 'Radioactivity': [2405], 'PhosphorImager': [2410], '(Molecular': [2411], 'Dynamics).': [2412], 'Membrane': [2413, 3917], '(5': [2415], 'mg/ml)': [2416], 'D,': [2421], 'μm': [2433, 2691, 3815, 3841], 'CuCl2,': [2434], 'MgCl2,': [2437], '10%': [2439, 2649], 'glycerol,': [2440, 2650], '2%': [2442, 3955], 'maltoside': [2444], '(DDM).': [2445], 'gently': [2449], 'shaken': [2450], 'h,': [2456], 'insoluble': [2459], 'fraction': [2460, 2474], 'removed': [2462, 2575], '250,000': [2466], 'h.': [2471], 'soluble': [2473], 'onto': [2477], 'Probond': [2479], 'Ni2+': [2480], 'column': [2482, 2492, 2582, 2590], '(Invitrogen)': [2483], 'pre-equilibrated': [2484, 2591], 'D': [2487, 2594], '0.1%': [2489, 2596, 2651], 'DDM.': [2490, 2597], 'bed': [2497, 2506], 'volumes': [2498, 2507], 'imidazole.': [2515, 2527], 'eluted': [2519], 'Fractions': [2528], 'collected': [2533], 'tubes': [2535], 'DTT': [2538, 3075, 3086, 3153, 3181, 4120, 4492, 4511, 4541, 4572], 'became': [2546, 5149], 'mm,': [2550], 'respectively.': [2551], 'immunoblotting.': [2561], 'CopA-containing': [2562], 'fractions': [2563], '10-fold': [2566], 'Centricon': [2568], 'concentrators': [2569], '(Millipore),': [2570], 'imidazole': [2573], 'filtration': [2578], '5-cm': [2581], 'filled': [2583], '3': [2585, 3376, 3542, 3695, 3754], 'Sephadex': [2588], 'G-25': [2589], 'buffers': [2599], 'degassed': [2601], 'under': [2602, 3944, 4884, 4897], 'vacuum': [2603], 'bubbled': [2605], 'Argon': [2607], 'before': [2608, 4984], 'steps': [2611], 'estimated': [2620], 'coupled': [2623], 'spectrometric': [2624], '(22Vogel': [2626], 'Steinhart': [2628], '1976;': [2631], '208-216Google': [2633], '(0.4': [2638], 'histidine,': [2643], '0.4': [2653], 'total': [2656], 'lipids': [2659], '(AVANTI': [2660], 'polar-lipids),': [2661], 'NADH,': [2664], '1.25': [2665], 'mmphosphoenolpyruvate,': [2666], '7': [2667, 4153], 'units': [2668, 2673], 'pyruvate': [2670], 'kinase,': [2671], 'lactate': [2675], 'dehydrogenase,': [2676], 'ATP.': [2679], 'Where': [2680], 'mmNaN3,': [2683], 'BCS,': [2686, 4080, 4107], 'μmCuCl2': [2688], '(acetonitrile': [2693], 'copper(I)': [2694, 3168], 'hexafluorophosphate,': [2695], 'Sigma)': [2696], 'added.': [2698], 'prior': [2710], 'initiating': [2712], 'signature': [2720], 'property': [2721], 'DKTG': [2734], 'accepts': [2736], 'γ-phosphate': [2738], 'cycle,': [2744], 'forming': [2745], 'covalent': [2747], '(23Pedersen': [2750, 4262], 'P.L.': [2751, 4263], 'Carafoli': [2752, 4264], 'Trends': [2754, 3625, 4266, 5122], '1987;': [2757, 4269], '12:': [2758, 4270], '146-150Google': [2759, 4271], 'investigate': [2762, 3029], 'CopA,': [2766, 3771, 4538], '(ΔcopA)': [2777], 'expressing': [2778, 3334, 3346, 3450, 3575, 3732, 5146, 5206], 'plasmid.': [2784], 'time-dependent': [2792], 'manner,': [2793], 'maximum': [2795], 'labeling': [2796, 3490], 'within': [2797, 2858], '(Fig.': [2800, 2819, 2939, 3072, 3132, 3357, 3375, 3694, 3707, 3739, 3753, 3767, 3787, 3997, 4046], 'A).': [2802, 3377, 3696, 3789, 3967], 'Vesicles': [2803], 'thecopA': [2805], 'exhibit': [2811], 'radioactive': [2813, 2935, 3056], 'band': [2814, 2936, 3057, 3992], 'position': [2818], '1,': [2820], 'lane': [2821, 2942, 3431, 3499], '1).': [2822, 2943], 'basic': [2828], '(Fig.1': [2832], 'B),': [2833, 4048], 'considered': [2836], 'reliable': [2839], 'criteria': [2840], 'presence': [2843, 2870, 3063, 3142, 3236, 3969, 4105, 4554, 4594, 4623], 'bond.': [2847], 'chased': [2852], 'unlabeled': [2856], 's,': [2860], 'transient': [2863], 'nature': [2864], 'intermediate.': [2867, 3601, 4743, 5227], 'μmsodium': [2873], 'orthovanadate,': [2874], 'mimics': [2876], 'transition': [2878], 'state': [2879, 2955], 'when': [2880], 'bound': [2881], 'active': [2884, 3986, 4065], 'site': [2885], 'ATPases,': [2888, 5271], 'entirely': [2892], 'inhibited.': [2893], 'On': [2894], 'other': [2896, 4124, 4404, 4598, 4911], 'hand,': [2897], 'azide,': [2904], 'inhibitor': [2906], 'many': [2908], 'ATPases.': [2909, 4914], 'Since': [2910, 3651, 4000, 4037, 4384, 5170], 'pump,': [2916], 'would': [2919, 4500], 'expected': [2921], 'ion.': [2927], 'ion,': [2933], 'observed': [2938, 3059, 3139, 3994], 'C,': [2941], 'date': [2945], 'there': [2946, 4012, 4108, 4715, 4776], 'been': [2948, 4276, 4382, 4398, 4449, 4515, 4724, 4904, 5025, 5109], 'clear': [2950, 4478], 'determination': [2951], 'redox': [2954], 'pumps,': [2962], 'is,': [2964], 'whether': [2965, 3030, 3150, 3397, 4479, 4858], 'both.': [2972, 3114, 4488], 'Our': [2973], 'previous': [2974], 'work': [2975], 'showed': [2976, 4471], 'reductant,': [2979], '18Fan': [3012], 'reductant': [3031, 3091, 3098], 'also': [3033, 3679, 4513, 5211], 'several': [3038, 4279], 'reductants': [3039], 'assay.': [3044], 'agreement': [3046], 'assays,': [3053, 4498], 'significant': [3055], 'reductant:': [3067], 'C).': [3074, 3134, 4197], 'GSH': [3077], 'effective': [3080, 4562], 'cysteine.': [3082], 'those': [3084, 3733], 'reasons': [3085], 'usually': [3088, 4629, 4636], 'assays.': [3094], 'reduce': [3102, 3108, 4501, 4518, 4828, 4837], 'reason': [3117], 'mono-': [3121], 'No': [3135, 3741], 'Co(II),': [3149], 'present.': [3155], '(added': [3162], 'acetonitrile': [3167], 'hexafluorophosphate)': [3169], 'showing': [3179], 'necessary': [3184, 4516], 'maintain': [3186], 'enzyme': [3188, 3595], 'reduced': [3191], 'state.': [3192], 'Moreover,': [3193], 'bathocuproindisulfonate': [3194], '(BCDS),': [3195], 'chelates': [3197], 'prevented': [3202], 'labeling.': [3203], 'demonstrate': [3206, 4345], 'divalent)': [3215], 'ATPase.': [3220, 4066], 'One': [3221], 'characteristic': [3222], 'differentiates': [3224], 'their': [3230, 4617, 4706, 4888, 4938], 'hard': [3231, 4618, 5267], 'binding': [3243, 4627, 4798, 5273, 5309], 'domains': [3244], 'cytosolic': [3247, 3388], 'region.': [3249, 4845], 'Gly-Met-Ser-Cys110-Ala-Ser-Cys113': [3265], 'although': [3277, 3396], 'extend': [3306], 'observation,': [3308], 'N-terminally': [3310], 'truncated': [3311, 3318, 3362], 'CopAs': [3312], 'constructed:': [3314], 'pCopA2ΔN1,': [3315], 'immediately': [3319], 'motifs': [3331, 3522, 4391, 4645, 4668, 4704, 4757, 4804, 4827, 4909, 4930, 4972], 'deleted.': [3332], 'ΔN1': [3335, 3465, 3564, 3778, 3882], 'retained': [3337], 'partial': [3338], 'CuSO4': [3341], 'vivo,': [3343], 'while': [3344], 'ΔN2': [3347, 3468, 3594], '2),': [3358, 3455], 'proteins': [3363, 3684, 3752, 4429, 5155], 'made': [3365, 4905], 'amounts': [3367, 3688], 'comparable': [3368, 3689], 'suggest': [3380], 'least': [3383], 'portion': [3385], 'necessary,': [3395], 'proper': [3403], 'folding': [3404], 'cannot': [3405], 'deduced': [3407], 'results.Figure': [3410], '3Acylphosphate': [3411], 'mutants.': [3416], 'assayed': [3421], 'legend': [3426], 'Fig.': [3428], '1.': [3429], 'μg': [3434], '(lane': [3445, 3454, 3457, 3460, 3463, 3466, 3469, 3472, 3475, 3479], '1)': [3446], 'bearing': [3448, 3591, 3924], 'plasmids': [3449], 'C14A/C17A': [3456], '3),': [3458], 'C110A/C113A': [3459], '4),': [3461], 'C14A/C17A/C110A/C113A': [3462, 3773], '5),': [3464], '6),': [3467], '7),': [3470], 'C479A': [3471, 3648], '8),': [3473], 'C481A': [3474], '9),': [3476], 'C481H': [3478, 3676, 3751, 5203], '10).': [3480], 'A,': [3481], 'immunoblotting': [3482, 3996], 'against': [3485], 'tag.': [3488], 'B,': [3489], '[γ-32P]ATP,': [3494], 'each': [3498, 3780], 'imaged': [3500], 'phosphorimager.View': [3503], 'Large': [3504], 'Image': [3505], 'Figure': [3506], 'ViewerDownload': [3507], '(PPT)': [3508], 'alanine': [3512, 5143], '(C14A/C17A)': [3517], '(C110A/113A)': [3520], 'substituted': [3528], '(C14A/C17A/C110A/C113A)': [3529], 'equivalent': [3534], '(Figs.1': [3539], 'B': [3540], 'B).': [3543, 3755, 3817, 3999], 'Indeed,': [3544], 'affect': [3559], 'intermediate,': [3570], 'copAconferred': [3578], 'vivo.': [3582, 4871], 'Consistent': [3583], 'distinctive': [3603, 4236, 5018], 'feature': [3604, 4237], 'highly': [3611, 4064], 'TM6,': [3616], 'putative': [3618, 4987], 'domain': [3620, 5117], '(24Solioz': [3621, 5118], 'Vulpe': [3623, 5120], '21:': [3629, 5126], '237-241Google': [3630, 5127], 'Cys481.': [3640], 'individually': [3645], 'mutated': [3646], 'C481A.': [3650], 'Enterococcus': [3653], 'hirae': [3654, 4441, 5176], 'CopB': [3655, 5172], 'Cys-Pro-His': [3658, 5179, 5220], '(6Odermatt': [3660, 5185], 'constructed.': [3680], 'three': [3682, 3793, 5010], 'expressed': [3686], 'vivo': [3706], 'A)': [3709], 'ATP-driven64Cu': [3711], 'examined.': [3716], 'None': [3717], 'genes': [3721, 3734], 'conferred': [3722], 'unable': [3736], 'accumulate64Cu': [3738], '4).': [3740], 'intermediates': [3743], 'detected': [3745], 'C479A,': [3748], 'C481A,': [3749], 'Formation': [3756], 'increasing': [3762, 3783], '5).': [3768], 'Wild': [3769], 'quadruple': [3774, 3873, 4745], 'mutant,': [3775], 'responded': [3781], 'bands': [3791], 'separate': [3794], '32P-labeled': [3795], 'averaged': [3804], 'and,': [3805], 'clarity,': [3807], 'value': [3812], '(Fig.5': [3816], 'K': [3820, 3869, 4189], '±': [3830, 3877, 3889], 'μm,': [3832, 3879], 'similar': [3835, 4206], 'theK': [3837], '3.9': [3840], 'Achaeoglobius': [3851], 'fulgidus': [3852, 4221, 4469], '(25Mandal': [3854, 4222, 4286], 'A.K.': [3855, 4223, 4287], 'Cheung': [3856, 4224, 4288], 'W.D.': [3857, 4225, 4289], 'Arguello': [3858, 4226, 4290], '2002;': [3863, 4231, 4295, 4325, 5086], '277:': [3864, 4232, 4296, 4326], '7201-7208Google': [3865, 4233, 4297], '0.45': [3876], 'apparentK': [3885], '0.84': [3888], 'Thus,': [3892, 5297], 'removal': [3893], 'modification': [3901], 'seemed': [3907], 'produce': [3909, 4850], 'increase': [3912], 'arabinose-induced': [3922], 'carries': [3928], 'control': [3945], 'promoter.': [3949], 'dodecylmaltoside,': [3956], 'chromatography': [3965], '(Fig.6': [3966, 4196], 'small': [3972], 'solubilization': [3977], 'purification': [3979], 'result': [3983, 4795], 'stable': [3988], 'minor': [3991, 4060], 'upon': [3995], 'slightly': [4002], 'smaller': [4003], 'reacted': [4005], 'tag,': [4011], 'probably': [4014], 'some': [4015, 4502], 'degradation': [4016], 'Although': [4023, 4962], 'hydrolysis': [4030], '80–90%': [4033], 'azide.': [4036], 'azide': [4045], 'it': [4049, 4366, 4424, 4475, 4855, 4996, 5091, 5097], 'likely': [4051], 'azide-sensitive': [4054], 'due': [4058, 4450], 'contamination': [4061, 4453], 'azide-insensitive': [4068, 4168], '130': [4072], 'nmol/mg/min': [4073, 4078], '65': [4077], 'chelator,': [4083], 'reflect': [4086, 4863], 'residual': [4087], 'remaining': [4089], 'purification.': [4091], 'Neither': [4092], 'nor': [4094], 'activity.': [4098], 'Compared': [4099], 'rate': [4102, 4135], '∼4-fold': [4111], 'activation': [4112], '(280': [4115], 'nmol/mg/min)': [4116], 'plus': [4119], '(295': [4121], 'nmol/mg/min).': [4122], 'experiments,': [4125], 'Ag(I)-stimulated': [4126, 4139, 4213], '4-': [4129], '5-fold': [4131], 'BCS.': [4137], 'relatively': [4143], 'broad': [4144], 'profile,': [4146], '8,': [4155], 'about': [4157, 4906], 'one-third': [4158], 'lower': [4159], '6.': [4162], 'indicate': [4165], 'catalyzed': [4172], 'requires': [4179], 'Cu(I)-stimulated': [4184, 4199], 'V': [4200, 4209], 'max': [4201, 4210], '0.19': [4203], 'μmol/mg/min': [4204], 'reported': [4215, 4526], 'homologue': [4219, 5173], 'fromA.': [4220], 'gave': [4242], 'its': [4245], 'name': [4246], 'cycle': [4261, 5265], '26Voskoboinik': [4299], 'Mar': [4301], '276:': [4311], '28620-28627Google': [4312], '27Tsivkovskii': [4314], 'Eisses': [4316], 'Lutsenko': [4320], '976-983Google': [4327], '28Wunderli-Ye': [4329], '280:': [4338], '713-719Google': [4339], 'Cu(I)-dependent': [4346], 'phosphorylation': [4347, 4497, 4536, 4591], 'Whereas': [4365], 'presumed': [4368], 'pumps': [4371, 4683, 4708], 'specific': [4373], 'proven.': [4383], 'peptide': [4387], 'disease': [4395], '(29DiDonato': [4407], '33279-33282Google': 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