Title: Physiology and Molecular Biology of a Family of Pathogenesis-Related PR-10 Proteins in Conifers
Abstract: Summary PR-10 is a group of pathognesis-related proteins that are induced by biotic and abiotic stresses. In conifers, PR-10 proteins and their genes were isolated and characterized. In some species, there is a multi-gene family encoding PR-10 proteins. These PR-10 proteins were shown to be up-regulated by pathogens in two conifer pathosystems and were associated with overwintering of conifers. In western white pine, the level of PR-10 protein is associated with frost hardines of white pine needles. Ultrastructural localization revealed that western white pine PR-10 protein is an intracellular protein and is found in chloroplasts of mesophyll and endodermis, and in the stroma. In infected pine needles, the PR-10 protein was bound to the cell surface of the blister rust pathogen. PR-10 genes are organized along two exons intervened by an intron. Homology of PR-10 proteins is higher among five-needle pines than in either Douglas-fir or white spruce. Cloning, sequencing, and functional analysis of PR-10 promoters are underway to identify cis-acting regulatory elements responsive to fungal infection and cold stress. Future research and possible applications are discussed.
Publication Year: 2004
Publication Date: 2004-05-24
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 20
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