Title: Calculation of hydrodynamic parameters of random coil polymers from size exclusion chromatography and comparison with parameters by conventional methods
Abstract: A new method of treating data from size exclusion chromatography, originally evaluated for native proteins, has been extended to random coil polymers using data from dextran and polyethylene glycol as specific examples. Columns are calibrated with globular proteins of known molecular weight. Multiplication by a constant transforms the abscissa to molecular radii. The constants in the equation r = gMZ are then evaluated directly from the size exclusion chromatographic data and compared with the corresponding constants obtained from viscometry, sedimentation velocity and light scattering, relating Stokes radius, root mean square end-to-end distance and radius of gyration to molecular weight.
Publication Year: 1981
Publication Date: 1981-06-01
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 53
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