Title: Age-related changes in the distribution pattern of glycosaminoglycans synthesized by cultured human diploid fibroblasts (WI-38)
Abstract: A gradual decline in the synthesis of glycosaminoglycans, as evidenced by reduced rates of incorporation of [35S]sulfate and [14C]glucosamine into cellular and medium glycosaminoglycans, was observed during the last (about 5) population doublings before phase-out. The decline was accompanied by a change in the distribution pattern of individual glycosaminoglycans with a relative decrease in the incorporation rate of [14C]glucosamine into cellular and medium hyaluronic acid. The incorporation rate of [14C]glucosamine and [35S]sulfate into cellular and medium heparan sulfate continually increased during the last population doublings ("senescence"). The possibility of a coupling between cell growth and hyaluronic acid synthesis or an involvement of hyaluronic acid in the adhesion of cells (among one another or/and to the substratum), and the functional significance of heparan sulfate as a growth inhibitor were discussed.
Publication Year: 1981
Publication Date: 1981-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 42
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