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'highly': [101, 687], 'divergent,': [102], 'GyV-related': [103], 'sequences': [104, 120, 275, 314, 337, 530, 540, 646, 669], 'human': [106, 124, 129, 316, 677, 693, 718, 758, 812], 'chicken': [108, 126, 697], 'biological': [109, 511], 'samples.': [110], 'Human': [111], 'GyV': [112, 116, 689], '1': [113, 387, 499, 552], '(HGyV1),': [114], 'avian': [115, 702], '2,': [117], 'GyV3': [119], 'identified': [122, 172, 456, 675], 'from': [123, 176, 190, 319, 498, 601, 607], 'skin,': [125], 'blood,': [127], 'feces,': [130], 'respectively': [131], '(2–4).': [132], 'These': [133], 'genomes': [134], 'harbor': [135], 'organization': [138], 'similar': [139], 'CAV,': [141], 'despite': [142], 'high': [144], 'divergence': [146], '(49%–65%).\r\n\r\nOne': [147], 'study': [148, 600, 681], 'detection': [151, 250, 450, 638, 747], 'GyVs': [153], 'HIV-positive': [155], 'patients': [156], 'kidney': [158], 'transplant': [159], 'recipients': [160], '(0.7%': [161], '6%,': [163], 'respectively)': [164], '(5),': [165], 'but': [166], 'these': [167, 526, 737], 'had': [169], 'not': [170, 611, 793], 'been': [171], 'blood': [174, 188, 192, 321, 495, 518, 577, 606], 'samples': [175, 189, 206, 436, 488, 496, 626, 695], 'healthy': [177, 191, 320, 579, 608], 'persons.': [178, 580], 'We': [179], 'investigated': [180], 'presence': [182, 784], 'HGyV': [184, 228, 274, 406, 481, 529], '352': [187, 434], 'donors': [193, 609], 'France': [195, 522], '(mean': [196], 'age': [197], '39': [198], 'years;': [199], '185': [200], 'men;': [201], 'M:F': [202], 'ratio': [203], '1:1.11).': [204], '\r\n\r\nPlasma': [205], 'prepared': [208], 'as': [209, 700], '(6),': [211], '1-mL': [213], 'aliquots': [214], 'used': [216], 'nucleic': [218], 'acids': [219], 'extraction': [220], '(MagNA': [221], 'pure': [222], 'LC;': [223], 'Roche': [224], 'Diagnostics,': [225], 'Meylan,': [226], 'France).': [227, 247], 'detected': [231], 'by': [232, 348, 381, 408, 445, 457], 'using': [233, 349, 409, 446, 458], '2': [234, 504], 'systems': [235, 748], 'separate': [237], 'real-time': [238, 430, 449], 'TaqMan': [239, 356, 397], 'amplification': [240, 416], 'assays': [241, 374, 398], '(StepOne': [242], 'Plus;': [243], 'Applied': [244], 'Biosystems,': [245], 'Courtaboeuf,': [246], 'The': [248, 264, 394, 581], 'first': [249], 'assay': [251, 266, 289], '(VP1': [252], 'gene)': [253], 'previously': [256], '(HGyV-rtFP/HGyV-rtRP': [257], 'primers,': [258], 'HGyV-rtP': [259], 'probe,': [260], '72': [261], 'nt)': [262], '(5).': [263, 615], 'second': [265], 'designed': [268, 640], 'following': [269], 'analysis': [271], 'available': [273], '(Figure):': [276], 'sense': [277], 'primer': [278, 282], 'HGyVsPBs': [279], '5′-GCTAAGACTGTRACATGGC-3′,': [280], 'reverse': [281], 'HGyVsPBr': [283], '5′-CTCCGGGAATAGCGTCTTC-3′,': [284], 'probe': [285], 'HGyVsPBp': [286], '5′-FAM-TGGCACTGGAGACACAGACTGCG-TAMRA-3′.': [287], 'This': [288, 616], 'targets': [290], 'VP2': [292, 655], 'gene': [293], 'genome,': [297], 'with': [298, 354, 525, 543, 701, 771, 778, 798], 'expected': [300], 'length': [301], '118–115': [303], 'bp,': [304], 'depending': [305], 'on': [306, 641], 'reference': [308], 'sequence': [309, 690], 'considered.': [310], '\r\n\r\n\r\n\r\nFigure\r\n\r\nAlignment': [311], 'partial': [313], 'gyroviruses': [317], '(HGyVs)': [318], 'donors,': [322], 'France.': [323, 598], 'Point': [324], 'mutation': [325, 554], 'corresponding': [326], 'nonsynonymous': [329, 558], 'substitution': [330], 'is': [331, 590, 665, 708, 721, 775], 'boldface': [333], '(13F1': [334], 'isolate).': [335], 'Reference': [336], 'GenBank': [339], 'accession': [340], 'nos.:': [341], 'HGyV1-915,': [342], '{type:entrez-nucleotide,attrs:{text:FR823283,term_id:334880274,term_text:FR823283}}': [343], '...\r\n\r\n\r\n\r\nAmplification': [344], 'reactions': [345], 'performed': [347], '10%': [350], 'extracted': [352], 'material': [353], 'Fast': [357], 'Universal': [358], 'PCR': [359, 431], 'Kit': [360], '(Applied': [361], 'Biosystems)': [362], 'final': [365], 'volume': [366], '20': [368, 378, 392], 'μL.': [369], 'Cycling': [370], 'conditions': [371], 'both': [373], '95°C': [376, 385], 's,': [379], 'followed': [380], '50': [382], 'cycles': [383], 's': [388], '60°C': [390], 's.': [393], 'sensitivity': [395], 'estimated': [400], '10': [403], 'copies': [404], 'dilutions': [410], 'synthetic': [413], 'template.': [414], 'Each': [415], 'product': [417], 'subjected': [419], 'additional': [421, 473], 'agarose': [422, 478], 'gel': [423, 479], 'electrophoresis': [424], 'help': [426], 'eliminate': [427], 'potential': [428, 760], 'false-negative': [429], 'results.\r\n\r\nAmong': [432], 'plasma': [435], 'tested,': [437], '(0.85%)': [439, 584], 'resulted': [440], 'positive': [443, 453, 487], 'signal': [444, 454], 'our': [447], 'in-house': [448], 'assay;': [451], 'no': [452], 'other': [460], 'system': [461, 639], 'tested.': [462], 'When': [463], 'tests': [465], 'repeated,': [467], 'identical': [468], 'results': [469, 568], 'obtained.': [471], 'No': [472, 510, 563], 'amplicons': [474], 'identifiable': [476], 'after': [477], 'analysis.': [480], 'titers': [483], '(<500': [491], 'copies/mL': [492], 'plasma).': [493], 'Positive': [494], 'originated': [497], 'woman': [500], '(age': [501], '44)': [502], 'men': [505], '(ages': [506], '29': [507], '39).': [509], 'or': [512, 551, 631, 720, 789], 'serologic': [513], 'marker': [514], 'evaluated': [515], 'routine': [517], 'donor': [519], 'screening': [520], 'associated': [524, 777], 'donations.': [527], '\r\n\r\nPartial': [528], 'obtained': [531], 'cloned': [533], 'sequenced': [535], '(7': [536], 'clones': [537], 'each).': [538], 'All': [539], 'characterized': [541], 'clustered': [542], 'HGyV1': [544, 645], 'sequences,': [545], 'exhibiting': [546], 'either': [547], '100%': [548], 'nucleotide': [549], 'identity': [550], 'point': [553], '(corresponding': [555], 'substitution,': [559], 'ala': [560], '→thr)': [561], '(Figure).': [562], 'intragenetic': [564], 'identified.\r\n\r\nOur': [567], 'demonstrate': [569], 'recently': [571], 'discovered': [572, 739], 'HGyVs': [573, 604, 671, 764, 786, 810], 'detectable': [575], 'prevalence': [583], 'suggests,': [585], 'infrequently': [591], 'found': [592], 'general': [595], 'population': [596], 'A': [599, 679, 729], 'Italy': [602], 'did': [610], 'detect': [612], 'such': [613], 'result': [617], 'may': [618], 'linked': [620], 'small': [623], 'number': [624], 'tested': [627], '(n': [628], '=': [629], '50)': [630], 'use': [634, 752], 'nonoptimized': [637], 'basis': [643], 'only;': [647], 'moreover,': [648], 'possibility': [650], 'VP1': [653], 'amplified': [656], 'regions': [657], 'would': [658], 'conserved': [660], 'differently': [661], 'must': [662], 'considered.\r\n\r\nIt': [664], 'probable': [666], 'subsequent': [668, 751], 'remain': [672], 'blood.': [678], 'recent': [680], 'reported': [682], 'divergent': [688], '(GyV4)': [691], 'fecal': [694], 'meat': [698], '(7);': [699], 'GyV2': [703], 'GyV3,': [705], 'further': [706], 'research': [707], 'needed': [709], 'determine': [711], 'whether': [712], 'variant': [714], 'replicates': [715], 'body': [719], 'solely': [722], 'ingested': [723], 'food': [725], 'passively': [727], 'excreted.': [728], 'better': [730], 'knowledge': [731], 'newly': [738], 'will': [741], 'enable': [742], 'development': [743], 'improved': [745], 'their': [750], 'epidemiologic': [754], 'studies': [755, 802], 'involving': [756], 'diverse': [757], 'cohorts.\r\n\r\nThe': [759], 'clinical': [761, 779], 'importance': [762], 'remains': [765], 'clarified.': [768], 'Although': [769], 'infection': [770], 'CAV': [772], 'birds': [774], 'frequently': [776], 'signs': [780], 'disease,': [782], 'immunocompromised': [788], 'immunocompetent': [790], 'humans': [791], 'does': [792], 'appear': [794], 'correlated': [797], 'visible': [799], 'symptoms.': [800], 'Further': [801], 'natural': [805], 'history': [806], 'distribution': [808], 'hosts': [813], 'needed.': [815]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2022109651', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 5}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 5}], 'updated_date': '2025-01-16T18:19:26.941362', 'created_date': '2016-06-24'}