Title: High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose
Abstract: An engineered strain MITXM-61 of Rhodococcus opacus PD630 capable of utilizing xylose was used to produce biofuel precursor-triacylglycerols (TAG) from mixed glucose–xylose substrates. Optimal nitrogen source and carbon source concentrations were investigated for cell growth and microbial lipid production by MITXM-61 in flask cultures. In a two-stage batch culture, the maximum lipid yield (0.152 g TAG per g consumed carbon source) was achieved by utilizing a mixture of glucose and xylose. The fed-batch culture featured intermittent feeding of refined xylose solution during the lipid accumulation stage provided 45 g L−1 of cell dry weight, 54% (g TAG per g cell dry weight) of lipid content, 0.179 g g−1 (g TAG per g consumed carbon source) of lipid yield, and a 6.9 g L−1 day−1 of lipid productivity. Fatty acids extracted from microbial lipids produced by MITXM-61 were predominately palmitic and oleic acids, which are the major components in TAG-derived biofuels.
Publication Year: 2015
Publication Date: 2015-01-17
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 37
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