Title: Erwinia amylovora strains from outbreaks of fire blight in Spain: phenotypic characteristics
Abstract: Annals of Applied BiologyVolume 146, Issue 1 p. 105-114 Erwinia amylovora strains from outbreaks of fire blight in Spain: phenotypic characteristics VICTORIA DONAT, VICTORIA DONAT Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, SpainSearch for more papers by this authorELENA G BIOSCA, ELENA G BIOSCA Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, Spain Current address: Departmento de Microbiología y Ecología, Universidad de Valencia, 46100 Burjassot, Valencia, SpainSearch for more papers by this authorARANTZA RICO, ARANTZA RICO Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorJAVIER PEÑALVER, JAVIER PEÑALVER Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, SpainSearch for more papers by this authorMARISA BORRUEL, MARISA BORRUEL Laboratorio Agrario, 31610 Villava, Navarra, SpainSearch for more papers by this authorDIONISIO BERRA, DIONISIO BERRA Laboratorio Agrario Fraisoro, 20159 Zizurkil, Guipúzcoa, SpainSearch for more papers by this authorTXARAN BASTERRETXEA, TXARAN BASTERRETXEA Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorJESÚS MURILLO, JESÚS MURILLO Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorMARÍA M LÓPEZ, Corresponding Author MARÍA M LÓPEZ Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, Spain *Corresponding Author E-mail: [email protected]Search for more papers by this author VICTORIA DONAT, VICTORIA DONAT Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, SpainSearch for more papers by this authorELENA G BIOSCA, ELENA G BIOSCA Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, Spain Current address: Departmento de Microbiología y Ecología, Universidad de Valencia, 46100 Burjassot, Valencia, SpainSearch for more papers by this authorARANTZA RICO, ARANTZA RICO Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorJAVIER PEÑALVER, JAVIER PEÑALVER Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, SpainSearch for more papers by this authorMARISA BORRUEL, MARISA BORRUEL Laboratorio Agrario, 31610 Villava, Navarra, SpainSearch for more papers by this authorDIONISIO BERRA, DIONISIO BERRA Laboratorio Agrario Fraisoro, 20159 Zizurkil, Guipúzcoa, SpainSearch for more papers by this authorTXARAN BASTERRETXEA, TXARAN BASTERRETXEA Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorJESÚS MURILLO, JESÚS MURILLO Universidad Pública de Navarra, 31006 Pamplona, Navarra, SpainSearch for more papers by this authorMARÍA M LÓPEZ, Corresponding Author MARÍA M LÓPEZ Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera, Km. 4,5, 46113 Moncada, Valencia, Spain *Corresponding Author E-mail: [email protected]Search for more papers by this author First published: 09 February 2005 https://doi.org/10.1111/j.1744-7348.2005.04079.xCitations: 13 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary One hundred and thirty strains of Erwinia amylovora recovered from Spanish foci of fire blight from 1995 to 2000 were characterised and compared to reference strains from different sources and origins. Their rapid identification was performed by double antibody sandwich indirect (DASI) ELISA, using specific monoclonal antibodies against E. amylovora, and molecular confirmation by PCR using primers specific to the native plasmid pEA29. The Spanish strains of E. amylovora grew on different general and selective media producing typical colonies, except one of them that was deficient in levan production, whereas none of them grew on minimal agar medium with copper sulphate and low content of asparagine. All of them were susceptible to tetracycline, streptomycin, kasugamycin and oxolinic acid. Biochemical characterisation of selected strains by API 20E system revealed a great homogeneity, with 80% of the Spanish strains showing one of the two majority API 20E profiles described for E. amylovora, and the remaining strains showing minor differences. Pathogenicity on pear fruits and hypersensitivity reaction was confirmed, but a delayed reaction was observed for two Spanish strains. This is the first characterisation of a large collection of Spanish strains of E. amylovora. References Anon. 2000. Council Directive 2000/29/EC of 8 May 2000 on protective measures against the introduction into the Community of organisms harmful to plants or plant products and against their spread within the Community. Official Journal of the European Communities, L169, 10 July 2000 43: 1– 112. Barny M A. 1995. Erwinia amylovora hrpN mutants, blocked in harpin synthesis, express a reduced virulence on host plants and elicit variable hypersensitive reactions on tobacco. 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Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis. Applied and Environmental Microbiology 63: 4421– 4426. Citing Literature Volume146, Issue1January 2005Pages 105-114 ReferencesRelatedInformation
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 26
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