Molecular and Biochemical Characterization of Two Plant Inositol Polyphosphate 6-/3-/5-Kinases

Name: Work Video:
Duration: 3 min 30 s
Jill Stevenson-Paulik; Audrey R. Odom John; John D. York
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'molecular': [23, 230, 285, 492], 'level': [24, 286, 3831], 'about': [25, 287, 1102], 'pathway(s)': [27, 289], 'involved': [28, 290], 'its': [30, 221, 292, 483, 604, 1037], 'production.': [31, 293], 'In': [32, 294, 705, 780, 910, 3767], 'budding': [33, 295, 1307], 'yeast,': [34, 296, 1448, 3867], 'IP6': [35, 297, 707, 953, 1219, 1300, 1322, 1449, 1522, 2969, 4013], 'synthesis': [36, 197, 234, 298, 459, 496, 633, 716, 1301], 'occurs': [37, 299], 'through': [38, 241, 300, 503, 575, 1110, 1311, 2466, 3977], 'sequential': [40, 302], 'phosphorylation': [41, 303, 2800, 4296], 'I(1,4,5)P3': [43, 134, 305, 396, 2331, 2359, 2799, 3959, 4126, 4196, 4364], 'by': [44, 83, 257, 306, 345, 519, 596, 957, 1323, 1555, 1710, 1750, 1887, 1967, 1983, 2007, 2228, 2413, 2434, 2463, 2621, 2680, 2772, 2786, 2843, 2882, 2912, 2999, 3040, 3238, 3474, 3787, 3950, 4178], 'two': [45, 69, 192, 307, 331, 454, 1327, 1443, 1499, 3794, 3813, 3957], 'gene': [46, 260, 308, 522, 1275], 'products,': [47, 309], 'Ipk2': [48, 172, 310, 434, 1368, 2070, 3496, 3851, 4003, 4080, 4227, 4253, 4287], 'and': [49, 55, 66, 78, 89, 102, 110, 117, 126, 170, 180, 214, 219, 244, 311, 317, 328, 340, 351, 364, 372, 379, 388, 432, 442, 476, 481, 506, 547, 651, 673, 728, 733, 820, 876, 951, 1036, 1043, 1060, 1138, 1148, 1273, 1330, 1414, 1504, 1542, 1701, 1705, 1741, 1775, 1803, 1841, 1857, 1872, 1944, 1978, 2023, 2025, 2095, 2112, 2149, 2183, 2224, 2244, 2291, 2296, 2298, 2317, 2325, 2327, 2397, 2439, 2461, 2483, 2494, 2549, 2573, 2608, 2659, 2707, 2784, 2809, 2834, 2852, 2880, 2896, 2937, 2951, 2980, 3020, 3024, 3066, 3071, 3083, 3128, 3228, 3263, 3279, 3296, 3318, 3338, 3379, 3407, 3435, 3469, 3499, 3800, 3806, 3823, 3840, 3845, 3852, 3868, 3896, 3932, 3960, 3971, 4004, 4029, 4033, 4078, 4125, 4132, 4138, 4202, 4279, 4284, 4361, 4369, 4371, 4397, 4408, 4416, 4420, 4427, 4436], 'Ipk1,': [50, 312], 'a': [51, 145, 157, 174, 229, 313, 407, 419, 436, 491, 541, 563, 571, 590, 629, 712, 809, 1297, 1312, 1370, 1690, 1915, 1958, 1989, 2046, 2247, 2276, 2280, 2385, 2467, 2522, 2885, 2897, 2940, 3013, 3205, 3343, 3452, 3480, 3483, 4084, 4316, 4336], 'IP3/IP4': [52, 314, 1372, 3862, 3996], 'dual-specificity': [53, 315, 1371, 3861, 3993], '6-/3-kinase': [54, 129, 316, 391, 1373], 'an': [56, 140, 207, 318, 402, 469, 555, 1020, 1417, 2132, 2403, 2699, 3292, 3978], '1,3,4,5,6-pentakisphosphate': [58, 320], '2-kinase,': [59, 321], 'respectively.': [60, 322, 2496, 3808, 4035], 'Here': [61, 323, 1490], 'we': [62, 165, 324, 427, 1491, 2312, 3773, 3924, 4025, 4088, 4106, 4272, 4314], 'report': [63, 166, 325, 428, 1492, 4250], 'identification': [65, 327], 'characterization': [67, 329, 1505], 'polyphosphate': [71, 333, 533, 2664, 2867, 3457, 3855, 4112], 'kinases': [72, 334, 3673, 3816, 3944, 3989, 4402], 'from': [73, 115, 335, 377, 611, 1001, 1228, 1266, 1377, 1882, 2258, 2556, 2585, 2595, 2601, 2900, 3122, 3359, 4123], 'Arabidopsis': [74, 336, 1712, 2018, 3777], 'thaliana,': [75, 337], 'designated': [76, 338, 2021, 2748, 4027], 'AtIpk2α': [77, 101, 125, 339, 363, 387, 2316, 2936, 2950, 4032, 4131, 4180, 4283, 4360, 4419], 'AtIpk2β': [79, 103, 127, 341, 365, 389, 2318, 2938, 2953, 4133, 4362, 4421], 'that': [80, 90, 131, 167, 246, 342, 352, 393, 429, 508, 565, 1009, 1025, 1218, 1374, 1493, 1501, 1511, 2240, 2251, 2256, 2305, 2826, 3674, 3818, 3883, 3986, 4075], 'are': [81, 91, 104, 343, 353, 366, 540, 739, 786, 949, 3820, 3842, 3859, 3871, 3884, 3948], 'encoded': [82, 256, 344, 518], 'distinct': [84, 346], 'genes': [85, 347, 1210, 1444, 1500], 'on': [86, 348, 2459, 2924, 4429], 'chromosome': [87, 349], '5': [88, 350, 2464, 3021, 3179], 'ubiquitously': [92, 354], 'expressed': [93, 355, 2313, 3934], 'mature': [95, 357], 'tissue.': [96, 358], 'The': [97, 359, 1028, 1693, 1768, 1782, 1819, 1855, 1907, 1972, 2088, 2135, 2167, 2212, 2270, 2284, 2364, 2423, 2451, 2497, 2513, 2536, 2676, 2876, 2993, 3079, 3234, 3247, 3284, 3324, 3363, 3391, 3419, 3446, 3835, 3888, 3939, 4183, 4324, 4399], 'primary': [98, 360, 3833], 'structures': [99, 361], '70%': [105, 367, 3821], 'identical': [106, 112, 368, 374, 2114, 3822, 3844], 'to': [107, 113, 120, 135, 155, 187, 369, 375, 382, 397, 417, 449, 551, 569, 606, 631, 725, 989, 1013, 1018, 1022, 1039, 1045, 1058, 1321, 1516, 1545, 1874, 1900, 2028, 2044, 2064, 2104, 2106, 2109, 2115, 2131, 2203, 2357, 2371, 2402, 2488, 2501, 2903, 2926, 3012, 3075, 3093, 3142, 3160, 3172, 3184, 3329, 3345, 3422, 3466, 3478, 3769, 3826, 3848, 4000, 4100, 4117, 4198, 4211, 4334, 4365, 4392, 4424, 4433], 'each': [108, 370, 1756, 2235, 2369, 3267, 3827, 4385], 'other': [109, 371, 803, 2854, 3828, 3874], '12–18%': [111, 373, 3843], 'Ipk2s': [114, 376], 'yeast': [116, 121, 171, 210, 378, 383, 433, 472, 1308, 1991, 2013, 4079, 4226, 4252, 4286], 'mammals.': [118, 380], 'Similar': [119, 381], 'Ipk2,': [122, 384], 'purified': [123, 385, 2243, 2326, 2350, 2729, 2862, 3930], 'recombinant': [124, 386, 2352, 2625, 2730, 2863, 2948, 3964, 4017, 4159, 4274, 4325], 'have': [128, 173, 390, 435, 656, 1142, 1211, 1216, 1513, 2061, 4026, 4073, 4303], 'activities': [130, 249, 392, 511, 1141], 'sequentially': [132, 394, 1319], 'phosphorylate': [133, 395, 2358, 3675, 4335, 4425], 'generate': [136, 398, 2045, 4081, 4212, 4434], 'I(1,3,4,5,6)P5': [137, 399, 1139, 1418, 3976, 4393, 4435], 'predominantly': [138, 400], 'via': [139, 401], 'I(1,4,5,6)P4': [141, 403, 4289, 4405], 'intermediate.': [142, 404], 'While': [143, 405], 'I(1,3,4,5)P4is': [144, 406], 'substrate': [146, 408, 1508, 3927, 4197, 4301], 'for': [147, 232, 409, 494, 603, 714, 1299, 1548, 1695, 1785, 1821, 1948, 2091, 2119, 2169, 2234, 2300, 2330, 2337, 2427, 2476, 2507, 2528, 2590, 2606, 2670, 2686, 2747, 2819, 2869, 2914, 2987, 3033, 3117, 3178, 3187, 3201, 3244, 3266, 3291, 3310, 3315, 3321, 3347, 3365, 3393, 3779, 4009, 4225, 4306, 4331, 4451], 'Ipk2s,': [150, 412], 'it': [151, 413, 1017, 2130, 3471, 4191, 4207], 'does': [152, 414, 4172], 'not': [153, 415, 2362, 2835, 3669, 4128, 4173, 4298, 4447], 'appear': [154, 416], 'be': [156, 255, 418, 517, 1019, 1222, 2110, 3479], 'detectable': [158, 420, 4020], 'product': [159, 421, 591, 1276, 2286, 2797, 2831, 3974, 4293, 4391], 'IP3': [162, 424, 625, 1378, 3467, 3489, 4099, 4137, 4295], 'reaction.': [163, 425, 4102], 'Additionally,': [164, 426], 'novel': [175, 437], '5-kinase': [176, 438, 4444], 'activity': [177, 439, 1279, 2068, 2333, 3998, 4011, 4349, 4445], 'toward': [178, 440], 'I(1,3,4,6)P4': [179, 441, 4426], 'I(1,2,3,4,6)P5,': [181, 443], 'which': [182, 444, 588, 655, 738, 785, 1317, 2592, 2610, 3858, 4114, 4157], 'would': [183, 445], 'allow': [184, 446], 'these': [185, 447, 1083, 1442, 4188, 4277], 'proteins': [186, 448, 2322, 2353, 2553, 4326], 'participate': [188, 450, 1517], 'least': [191, 453], 'proposed': [193, 455], 'pathways': [194, 456, 1116, 1226, 1520], 'IP6.': [199, 461, 3961], 'Heterologous': [200, 462], 'expression': [201, 463, 1955, 2014], 'either': [203, 465, 1111, 1440, 2347, 2997, 3049, 3963, 4179], 'isoform': [205, 467], 'ipk2': [208, 470], 'mutant': [209, 471], 'strain': [211, 473], 'restores': [212, 474], 'IP4': [213, 475, 2700, 2796, 2833, 3979, 4094, 4142, 4175, 4366, 4390], 'IP5': [215, 477, 1376, 4101], 'production': [216, 478, 1092, 1107], 'vivo': [218, 480], 'rescues': [220, 482], 'temperature-sensitive': [222, 484], 'growth': [223, 485], 'defects.': [224, 486], 'Collectively': [225, 487], 'our': [226, 488, 4441], 'results': [227, 489, 4221], 'provide': [228, 490], 'basis': [231, 493], 'higher': [236, 498, 640, 782], 'polyphosphates': [238, 500, 723], 'plants': [240, 502, 1109, 3134], 'multiple': [242, 504, 1519], 'routes': [243, 505], 'indicate': [245, 507], '6-/3-/5-kinase': [248, 510], 'found': [250, 512, 3670, 3793, 4280], 'extracts': [253, 515], 'may': [254, 516, 1220], 'IPK2': [259, 521, 1329], 'class.': [261, 523], '1,4,5-trisphosphate': [525, 586], 'performance': [527], 'liquid': [528], 'chromatography': [529], 'glutathione': [530, 1949, 2566], 'S-transferase': [531, 1950], '5-phosphatase': [534, 2665, 2868, 4162, 4170, 4184], 'catalytically': [535], 'inactive': [536], 'kinase': [537, 1140, 1265, 2034, 2067, 2332, 3878, 3997, 4010, 4311], 'Phosphorylation': [538], 'events': [539], 'vital': [542], 'part': [543], 'intracellular': [545, 583, 612], 'communication': [546], 'enable': [548], 'specific': [549], 'responses': [550], 'various': [552, 867, 2030], 'stimuli.': [553], 'As': [554, 3342, 4165, 4358], 'asymmetric': [556], 'cyclitol': [557], 'harboring': [558, 3802, 3995], 'six': [559], 'hydroxyls,': [560], 'myo-inositol': [561], 'scaffold': [564], 'has': [566, 599, 708, 1270, 1277, 1302, 1498, 4446], 'potential': [568, 1515], 'transduce': [570], 'wealth': [572], 'information': [574], 'combinatorial': [577], 'addition': [578, 2415, 2774, 2845, 3001, 4374], 'phosphates.': [580], 'One': [581, 2378], 'such': [582, 643, 664, 869], 'messenger,': [584], '(IP3),1': [587], 'phosphatidylinositol': [593], '4,5-bisphosphate': [594], 'hydrolysis': [595], 'phospholipase': [597, 1313], 'C,': [598], 'been': [600, 658, 709, 1143, 1212, 1271, 1303, 2062, 4304, 4448], 'extensively': [601], 'characterized': [602, 3860], 'ability': [605, 1038, 4333], 'specifically': [607], 'stimulate': [608], 'calcium': [609], 'release': [610], 'stores': [613], '(1Berridge': [614], 'M.J.': [615, 695, 3549], 'Nature.': [616, 1237], '1993;': [617], '361:': [618], '315-325Crossref': [619], 'PubMed': [620, 687, 751, 775, 832, 887, 905, 968, 1077, 1130, 1171, 1191, 1241, 1255, 1291, 1346, 1363, 1391, 1409, 1434, 1468, 1485, 1573, 1736, 1941, 2083, 3111, 3526, 3543, 3565, 3590, 3619, 3644, 3660, 3698, 3722, 3742, 3762, 3918, 4050, 4068, 4242, 4267], 'Scopus': [621, 688, 701, 752, 776, 833, 846, 888, 906, 969, 982, 1078, 1131, 1172, 1201, 1242, 1256, 1292, 1347, 1364, 1392, 1410, 1435, 1469, 1486, 1737, 2084, 3112, 3527, 3544, 3566, 3591, 3620, 3645, 3661, 3699, 3723, 3743, 3763, 3919, 4051, 4069, 4243, 4268], '(6188)': [622], 'Google': [623, 690, 703, 754, 778, 835, 848, 890, 908, 971, 984, 1080, 1133, 1174, 1192, 1203, 1244, 1258, 1294, 1349, 1366, 1394, 1412, 1437, 1471, 1488, 1574, 1739, 1942, 2086, 3114, 3529, 3546, 3568, 3593, 3622, 3647, 3663, 3701, 3725, 3745, 3765, 3921, 4053, 4071, 4245, 4270], 'Scholar).': [624, 704, 779, 849, 909, 941, 1081, 1134, 1259, 1295, 1367, 1438, 1489, 2087, 3115, 3664, 3766, 3922, 4246], 'serves': [627], 'precursor': [630], 'over': [635, 2884, 2908], '15': [636, 2508, 2529], 'water-soluble': [637], 'IPs': [638, 790, 2582, 2598, 2920, 3089, 4320], 'including': [639], 'phosphorylated': [641, 1320, 2620, 2939, 4363, 4388, 4404], 'species': [642, 1269, 4342], 'tetrakisphosphate': [646], '(IP4),': [647], 'pentakisphosphate': [649], '(IP5),': [650], 'recently': [657], 'implicated': [659], 'messengers': [661], 'regulating': [662], 'processes': [663], 'mRNA': [666], 'export,': [667], 'transcription,': [668], 'DNA': [669, 3305], 'repair,': [670], 'channel': [671], 'activity,': [672], 'membrane': [674], 'trafficking': [675], '(reviewed': [676], 'Refs.': [678], '2Majerus': [679], 'P.W.': [680, 1283, 3734], 'Annu.': [681], 'Rev.': [682], 'Biochem.': [683, 828, 883, 964, 1250, 1284, 3735, 3913, 4063], '1992;': [684, 1168], '61:': [685], '225-250Crossref': [686], '(349)': [689], 'Scholarand': [691], '3Irvine': [692], 'R.V.': [693], 'Schell': [694], 'Nature': [696], 'Reviews.': [697], '2001;': [698, 897, 1069, 1122, 3616], '2:': [699], '327-338Crossref': [700], '(542)': [702], 'addition,': [706], 'suggested': [710, 2227], 'reservoir': [713], 'diphosphoryl': [719, 3500, 4021], 'class': [720, 3994], '(referred': [724], 'PP-IPs': [727], 'some': [730], 'cases': [731], 'IP7': [732], 'IP8)': [734], 'functions': [736], 'currently': [740], 'under': [741, 2714, 4345], 'investigation': [742], '(4Shears': [743], 'S.B.': [744, 763, 1403, 3514, 4044, 4261], 'Biochim.': [745], 'Biophys.': [746, 1285, 3736], 'Acta.': [747], '1998;': [748, 1198, 1728, 1933], '1436:': [749], '49-67Crossref': [750], '(153)': [753], 'Scholar,': [755, 836, 891, 972, 1175, 1193, 1245, 1350, 1395, 1472, 3530, 3547, 3569, 3594, 3623, 3648, 3702, 3746, 4054], '5Saiardi': [756], 'A.': [757, 1354, 1382, 1397, 1476, 1496, 1567, 2074, 2705, 3132, 3508, 3534, 3571, 3596, 3602, 3615, 4038, 4255], 'Caffrey': [758, 1398, 3509, 4039, 4256], 'J.J.': [759, 1399, 3510, 4040, 4257], 'Snyder': [760, 1400, 3511, 3578, 3607, 4041, 4258], 'S.H.': [761, 1401, 3512, 3579, 3608, 4042, 4259], 'Shears': [762, 1402, 3513, 4043, 4249, 4260], 'J.': [764, 1182, 1251, 1568, 1725, 2708, 3515, 3555, 3706, 3711, 3751, 3914, 4064], 'Biol.': [765, 1183, 1726, 1932, 3516, 3581, 3712, 3752], 'Chem.': [766, 1184, 1727, 3517, 3713, 3753], '2000;': [767, 843, 979, 1360, 1388, 1406, 1482, 2080, 3518, 3540, 3714, 4047, 4264], '275:': [768, 3519, 3715], '24686-24692Abstract': [769, 3520], 'Full': [770, 772, 900, 902, 1072, 1074, 1125, 1127, 1188, 1731, 1733, 1936, 1938, 3521, 3523, 3585, 3587, 3717, 3719, 3757, 3759], 'Text': [771, 773, 901, 903, 1073, 1075, 1126, 1128, 1189, 1732, 1734, 1937, 1939, 3522, 3524, 3586, 3588, 3718, 3720, 3758, 3760], 'PDF': [774, 904, 1076, 1129, 1190, 1735, 1940, 3525, 3589, 3721, 3761], '(155)': [777, 3528], 'plants,': [781], 'polyphosphates,': [784], 'generally': [787], 'defined': [788], 'with': [791, 1758, 1914, 1945, 1988, 2000, 2199, 2279, 2289, 2339, 2384, 2518, 2534, 2542, 2559, 2695, 2737, 2813, 2858, 2917, 2957, 3157, 3181, 3873, 3969, 4136, 4372, 4382], 'four': [792], 'more': [794, 916], 'phosphates,': [795], 'likely': [796], 'share': [797], 'similar': [798, 3825, 3847, 4220], 'signaling': [799], 'roles': [800], 'eukaryotes,': [804], 'but': [805, 1205, 4127], 'they': [806, 1512, 3819], 'serve': [808], 'specialized': [810], 'function': [811], 'seeds': [813, 924], 'storage': [815, 860, 946], 'pools': [816], 'inositol,': [818], 'phosphate,': [819], 'mineral': [821, 1041, 1049], 'nutrients': [822, 988], '(6Raboy': [823, 878, 959], 'V.': [824, 879, 893, 926, 960, 1065, 1118, 3652], 'Gerbasi': [825, 880, 961], 'P.': [826, 881, 962, 3577, 3750], 'Subcell.': [827, 882, 963], '1996;': [829, 884, 965, 1252, 3754], '26:': [830, 885, 966], '257-285Crossref': [831, 886, 967], '(85)': [834, 889, 970], '7Loewus': [837, 973], 'F.A.': [838, 932, 974], 'Murthy': [839, 975], 'P.P.N.': [840, 976], 'Plant': [841, 895, 977, 1067, 1120, 1196, 3149], 'Science.': [842, 978, 1342, 1359, 1387, 1430, 1464, 1481, 2079, 3107, 3539, 3640, 3694, 4238], '150:': [844, 980], '1-19Crossref': [845, 981], '(521)': [847, 983], 'During': [850, 942], 'seed': [851], 'development,': [852], 'IP6,': [853, 4437], 'phytate,': [857], 'accumulates': [858], 'protein': [861, 947, 3484, 3966, 4018], 'bodies': [862, 948], 'mixed': [864], 'salts': [865], 'minerals': [868], 'iron,': [871], 'calcium,': [872], 'magnesium,': [873], 'potassium,': [874], 'manganese,': [875], 'zinc': [877], '8Raboy': [892], 'Trends': [894, 1066, 1119], 'Sci.': [896, 1068, 1121, 3612], '6:': [898, 1070, 1123], '458-462Abstract': [899, 1071, 1124], '(277)': [907, 1079, 1132], 'this': [911, 1580, 2059, 2273, 3666, 3780, 3946, 4223, 4443], 'form': [912], 'often': [914], 'represents': [915], 'than': [917], '1%': [918], 'dry': [921], 'weight': [922], '(9Raboy': [925], 'Morre': [927], 'D.J.': [928], 'Boss': [929, 1723], 'W.F.': [930, 1724], 'Loewus': [931], 'Inositol': [933], 'Metabolism': [934], 'Plants.': [936], 'Wiley-Liss,': [937], 'Inc.,': [938], '1990:': [939], '55-76Google': [940], 'germination,': [943], 'phytate-containing': [945], 'disassembled,': [950], 'rapidly': [955], 'hydrolyzed': [956, 4209], 'phytases': [958], 'Scholar)': [985, 1943, 4072], 'releasing': [986], 'stored': [987, 2574], 'developing': [991], 'seedling': [992], 'before': [993], 'capable': [997], 'absorbing': [999], 'them': [1000, 2329], 'immediate': [1003], 'environment.': [1004], 'Ironically,': [1005], 'very': [1007, 1098], 'properties': [1008], 'make': [1010], 'beneficial': [1012], 'cause': [1016], 'antinutrient': [1021], 'animal': [1024, 1046, 1054], 'consumes': [1026], 'it.': [1027], 'concentration': [1030, 3015], 'food': [1034], 'grains': [1035], 'chelate': [1040], 'cations': [1042], 'resistance': [1044], 'digestion': [1047], 'compromises': [1048], 'absorption.': [1050], 'Undigested': [1051], 'waste': [1055], 'contributes': [1057], 'eutrophication': [1059], 'environmental': [1061], 'phosphorus': [1062], 'pollution': [1063], '(8Raboy': [1064, 1117], 'For': [1082, 2721], 'reasons,': [1084], 'there': [1085], 'significant': [1087, 1095], 'interest': [1088], 'altering': [1090], 'agriculturally': [1094], 'plants.': [1096], 'Currently,': [1097], 'genetics': [1104], 'lipid': [1112], 'dependent': [1113], 'independent': [1115, 2232], 'Biochemically,': [1135], 'I(1,4,5)P3,': [1136, 2960, 4353], 'I(1,3,4,5)P4,': [1137, 2964, 4355], 'described': [1144, 1718, 2210, 2335, 2719, 2817, 3043, 3356, 3463, 4305, 4344, 4449], 'pea': [1146], 'roots': [1147], 'immature': [1149], 'soybean': [1150], 'seeds,': [1151], 'respectively,': [1152, 1866], '(10Chattaway': [1153], 'J.A.': [1154], 'Drobak': [1155], 'B.K.': [1156], 'Watkins': [1157], 'P.A.C.': [1158], 'Dawson': [1159], 'A.P.': [1160], 'Letcher': [1161, 3550], 'A.J.': [1162, 3551], 'Stephens': [1163], 'L.R.': [1164, 1234], 'Irvine': [1165, 1235, 3558], 'R.F.': [1166, 1236, 3559], 'Planta.': [1167], '187:': [1169], '542-545Crossref': [1170], '(22)': [1173], '11Phillippy': [1176], 'B.Q.': [1177, 1195], 'Ullah': [1178], 'A.H.': [1179], 'Ehrlich': [1180], 'K.C.': [1181], '1994;': [1185], '269:': [1186], '28393-28399Abstract': [1187], '12Phillippy': [1194], 'Physiol.': [1197], '116:': [1199], '291-297Crossref': [1200], '(25)': [1202], 'Scholar),': [1204, 1413, 1740, 3726, 4271], 'none': [1206], 'corresponding': [1209], 'identified.': [1213], 'Several': [1214], 'studies': [1215], 'indicated': [1217, 3985], 'synthesized': [1223, 1453, 1715], 'lipid-independent': [1225], 'directly': [1227, 3358], 'and/or': [1230], '3-phosphate': [1232], '(13Stephens': [1233], '1990;': [1238, 3641], '346:': [1239], '580-583Crossref': [1240], '(131)': [1243], '14Brearley': [1246], 'C.A.': [1247, 3553], 'Hanke': [1248], 'D.E.': [1249], '314:': [1253], '227-233Crossref': [1254], '(88)': [1257], 'this,': [1261, 4105], 'only': [1262, 2253], 'one': [1263], 'IP': [1264, 2033, 2055, 2066, 2931, 3501, 3672, 3815, 3900, 3943, 4022, 4310, 4401], 'any': [1267, 3162, 4452], 'cloned': [1272], 'thisArabidopsis': [1274], 'I(1,3,4)P35/6-kinase': [1278], '(15Wilson': [1280, 3731], 'M.P.': [1281, 3732, 3748], 'Majerus': [1282, 3733, 3749], 'Res.': [1286, 3737], 'Commun.': [1287, 3738], '1997;': [1288, 3739, 3915], '232:': [1289, 3740], '678-681Crossref': [1290, 3741], '(55)': [1293, 3744], 'Recently': [1296], 'pathway': [1298, 1315], 'delineated': [1304], 'Saccharomyces': [1309], 'cerevisiae': [1310, 2032, 3046, 3850, 4002], 'C-dependent': [1314], 'IP3is': [1318], 'products': [1325, 1861, 2239, 3955], 'genes,': [1328], 'IPK1': [1331], '(16York': [1332, 1420, 1454, 3097, 3684, 4228], 'J.D.': [1333, 1358, 1386, 1421, 1455, 1480, 2078, 3098, 3538, 3685, 3710, 4229], 'Odom': [1334, 1422, 1456, 3099, 3686, 4230], 'A.R.': [1335, 1352, 1380, 1423, 1457, 1474, 2072, 3100, 3532, 3687, 4231], 'Murphy': [1336, 1424, 1458, 3101, 3688, 4232], 'R.': [1337, 1425, 1459, 2704, 3102, 3689, 4233], 'Ives': [1338, 1426, 1460, 3103, 3690, 4234], 'E.B.': [1339, 1427, 1461, 3104, 3691, 3704, 4235], 'Wente': [1340, 1355, 1383, 1428, 1462, 1477, 2075, 3105, 3535, 3692, 3707, 4236], 'S.R.': [1341, 1356, 1384, 1429, 1463, 1478, 2076, 3106, 3536, 3693, 3708, 4237], '1999;': [1343, 1431, 1465, 3108, 3562, 3582, 3695, 4239], '285:': [1344, 1432, 1466, 3109, 3696, 4240], '96-100Crossref': [1345, 1433, 1467, 3110, 3697, 4241], '(448)': [1348, 1436, 1470, 3113, 3700, 4244], '17Odom': [1351, 1473, 3531], 'Stahlberg': [1353, 1381, 1475, 2073, 3533], 'York': [1357, 1385, 1479, 2077, 3094, 3537, 3709], '287:': [1361, 1389, 1483, 2081, 3541], '2026-2029Crossref': [1362, 1390, 1484, 2082, 3542], '(346)': [1365, 1393, 1487, 2085, 3545], 'generates': [1375], '(17Odom': [1379, 2071], '18Saiardi': [1396], 'FEBS': [1404, 3560, 4045, 4262], 'Lett.': [1405, 3561, 4046, 4263], '468:': [1407, 4048, 4265], '28-32Crossref': [1408, 4049, 4266], '(119)': [1411, 4052, 4269], 'Ipk1': [1415], '2-kinase': [1419, 2701], 'When': [1439, 3864, 3962, 4007], 'dysfunctional': [1446], 'no': [1451], 'longer': [1452], 'thaliana': [1497, 3133], 'encode': [1502], 'IP3/IP4kinases,': [1503], 'their': [1507], 'specificities': [1509, 4302], 'suggests': [1510], 'synthesis.': [1523], 'Yeast': [1524, 1551], 'strains': [1525, 1577], '(described': [1526], 'TableI)': [1528], 'were': [1529, 1553, 1708, 1748, 1867, 1885, 1909, 1981, 2020, 2026, 2102, 2113, 2138, 2171, 2195, 2215, 2241, 2354, 2368, 2425, 2453, 2486, 2554, 2583, 2599, 2611, 2734, 2770, 2811, 2878, 2921, 2954, 3038, 3055, 3081, 3155, 3236, 3286, 3327, 3353, 3367, 3395, 3424, 4134, 4327, 4422], 'grown': [1530, 2398, 2426, 3056], 'complete': [1532, 3061], 'minimal': [1533, 3062], 'medium': [1534, 3063], 'containing': [1535, 2448, 2631, 2755, 3067, 3087, 3208], 'appropriate': [1537], 'amino': [1538], 'acids,': [1539], '2%': [1540], 'glucose,': [1541], 'lacking': [1543, 3064], 'uracil': [1544, 3065], 'maintain': [1546], 'selection': [1547], 'URA3': [1549, 1998], 'plasmids.': [1550], 'transformations': [1552], 'performed': [1554, 2216, 3354], 'lithium': [1557], 'acetate': [1558], 'method': [1559, 4224], '(19Ito': [1560], 'H.': [1561, 3557, 3573], 'Fukuda': [1562], 'Y.': [1563], 'Murata': [1564], 'K.': [1565, 3906], 'Kimura': [1566], 'Bacteriol.': [1569], '1983;': [1570], '153:': [1571], '163-168Crossref': [1572], 'Scholar).Table': [1575], 'IYeast': [1576], 'used': [1578, 1784, 1873, 2027, 2043, 2196, 2202, 2245, 2370, 3326, 3421, 4107], 'studyStrainGenotypeW303aMATα': [1581], 'ade2–1': [1582, 1588, 1595, 1603, 1610, 1618, 1626, 1634, 1642, 1651, 1660, 1669, 1678], 'ura3–1': [1583, 1589, 1596, 1604, 1611, 1619, 1627, 1635, 1643, 1652, 1661, 1670, 1679], 'his3–11,15': [1584, 1590, 1597, 1605, 1612, 1620, 1628, 1636, 1644, 1653, 1662, 1671, 1680], 'trp1–1': [1585, 1591, 1598, 1606, 1613, 1621, 1629, 1637, 1645, 1654, 1663, 1672, 1681], 'leu2–3,112': [1586, 1592, 1599, 1607, 1614, 1622, 1630, 1638, 1646, 1655, 1664, 1673, 1682], 'can-100AOY138MATα': [1587], 'can-100': [1593, 1600, 1608, 1615, 1623, 1631, 1639, 1647, 1656, 1665, 1674, 1683], 'ipk2∷kanRAOY108MATα': [1594], 'ipk1∷kanR': [1601, 1648, 1657, 1666, 1675, 1684], 'ipk2∷HIS3AOY60MATα': [1602], 'ipk1∷kanRkcs1∷HIS3JSY30MATα': [1609], 'ipk2∷kanR': [1616, 1624, 1632], 'pRS316(URA3)JSY31MATα': [1617], 'pRSAtIPK2α/URA3JSY32MATα': [1625], 'pRSAtIPK2β/URA3JSY36MATα': [1633], 'ipk1∷kanRipk2∷HIS3': [1640], 'pRS316(URA3)JSY37MATα': [1641], 'ipk2∷HIS3': [1649, 1658], 'pRSAtIPK2α/URA3JSY38MATα': [1650], 'pRSAtIPK2β/URA3JSY39MATα': [1659], 'kcs1∷HIS3': [1667, 1676, 1685], 'pRS316(URA3)JSY40MATα': [1668], 'pRSAtIPK2α/URA3JSY41MATα': [1677], 'pRSAtIPK2β/URA3': [1686], 'Open': [1687], 'table': [1688], 'new': [1691], 'tab': [1692], 'cDNAs': [1694, 1908, 1980, 2019], 'coding': [1697, 1743], 'regions': [1698], 'AtIPK2α': [1700, 1746, 1786, 1863, 1977, 2003, 2092, 3366], 'AtIPK2β': [1702, 1824, 1979, 2005, 2170, 3394], '(GenBankTMaccession': [1703, 3340], 'AJ404678': [1704, 3799, 3839, 4028], 'AJ245521,': [1706], 'respectively)': [1707], 'generated': [1709, 2237, 2612, 4177], 'RT-PCR.': [1711], 'cDNA': [1713, 2117, 2278], 'was': [1714, 2042, 2093, 2097, 2275, 2287, 2382, 2411, 2499, 2515, 2538, 2593, 2619, 2678, 2693, 2801, 2832, 2841, 2856, 2996, 3010, 3027, 3090, 3120, 3139, 3170, 3461, 3472, 3967, 3975, 4144, 4163, 4185, 4350], 'previously': [1717, 4218, 4450], '(20Stevenson': [1719], 'J.M.': [1720], 'Perera': [1721], 'I.Y.': [1722], '273:': [1729], '22761-22767Abstract': [1730], '(128)': [1738], 'sequences': [1744, 3837, 3870], 'andAtIPK2β': [1747, 1864], 'amplified': [1749, 2257], 'PCR': [1751, 1763, 1860, 2040, 2100, 2213, 2238, 2249, 2285, 3249, 3351], 'using': [1752, 2217, 2723, 3146, 3782, 3956, 4012, 4222, 4352], '25': [1753, 2532, 2915], 'pmol': [1754], 'primer': [1757, 3168], 'Expand': [1760], 'High': [1761], 'Fidelity': [1762], 'System': [1764], '(Roche': [1765, 3273], 'Molecular': [1766, 3274], 'Biochemicals).': [1767], 'sense': [1769, 3262], 'primers': [1770, 1778, 1783, 1820, 2101, 2136, 2168, 2194, 2201, 2255, 3265, 3325, 3364, 3392, 3420], 'contained': [1771, 1779, 2252, 3251], '5′-NcoI': [1772], 'restriction': [1773], 'sites': [1774], 'antisense': [1777, 3264], '5′-SalI': [1780], 'sites.': [1781], 'were:': [1787, 1825], 'sense,': [1788, 1826, 2141, 2174], '5′-AA': [1789, 1805, 1827, 1843], 'ACC': [1790, 1828, 3402], 'ATG': [1791, 1793, 1829, 1831, 2144, 2177], 'GCG': [1792, 1830], 'CAG': [1794, 1801, 1812], 'CTC': [1795, 1832, 3386, 3409], 'AAA': [1796], 'GTC': [1797, 1834], 'CCT': [1798, 1808, 1835, 1846, 3443], 'GAA': [1799, 1836, 3437], 'CAT': [1800, 1814, 2152, 2186], 'G-3′': [1802, 1840, 2182], 'antisense,': [1804, 1842, 2150, 2184], 'AGT': [1806, 1844, 3413], 'CGA': [1807, 1845, 2154, 2188, 3438], 'AAG': [1809, 1833, 2147, 2180, 3371, 3403, 3410], 'AAT': [1810], 'CTG': [1811, 1815, 3373, 3400, 3440, 3442], 'ACT': [1813], 'GGC': [1816], 'TCT': [1817, 1850, 3384], 'G-3′.': [1818], 'amplification': [1822, 2274], 'CAC': [1837], 'CAA': [1838, 1851, 2155, 2189, 3432], 'GTT': [1839, 2146, 2179], 'AGC': [1847, 3369], 'GCC': [1848], 'CGT': [1849], 'GTA': [1852, 2143, 2176, 3387], 'GGG': [1853, 3385], 'AAG-3′.': [1854], '861': [1856], '903': [1858], 'bp': [1859], 'representing': [1862], 'cDNA,': [1865, 3257], 'subcloned': [1868], 'into': [1869, 2294, 3991], 'pCR-Blunt': [1870], '(Invitrogen)': [1871], 'transform': [1875, 2029, 2372], 'competent': [1876, 2373], 'TOP10': [1877], 'Escherichia': [1878], 'coli.': [1879], 'Purified': [1880], 'plasmids': [1881, 1999, 2367], 'positive': [1883], 'transformants': [1884], 'sequenced': [1886], 'ABI': [1889], 'Prism': [1890], 'dGTP': [1891], 'Big': [1892], 'Dye': [1893], 'Terminator': [1894], 'Ready': [1895], 'Reaction': [1896], 'kit': [1897], '(PE': [1898], 'BioSystems)': [1899], 'verify': [1901, 2304], 'integrity': [1903], 'cDNA.': [1906, 2269], 'then': [1910, 2516, 3297], 'individualy': [1911], 'ligated': [1912, 2293], 'in-frame': [1913], 'Lox': [1916], 'recombination': [1917, 1987], 'site': [1918, 2090, 3902], 'pUNI-10': [1920, 1973, 2295], '(21Liu': [1921], 'Q.,': [1922], 'Li,': [1923], 'M.Z.': [1924], 'Leibham': [1925], 'D.': [1926, 1928, 2709, 3650], 'Cortez': [1927], 'Elledge': [1929], 'S.J.': [1930], 'Curr.': [1931, 3580], '8:': [1934], '1300-1309Abstract': [1935], 'sequence': [1947, 3804, 3895], '(GST)': [1951], 'bacterial': [1954], 'vector,': [1956], 'pGEX-KG,': [1957, 2297], 'modified': [1959], 'version': [1960], 'pGEX-2T': [1962], '(Amersham': [1963, 2526], 'Biosciences),': [1964], 'kindly': [1965], 'provided': [1966, 3786], 'Danny': [1968], 'Lew,': [1969], 'Duke': [1970], 'University.': [1971], 'vectors': [1974, 2015], 'carrying': [1975, 2016, 2392, 3048], 'recombined': [1982], 'vitro': [1985, 4330], 'site-specific': [1986], 'pLox/MYC3/URA3/CEN': [1990], 'host': [1992], 'vector': [1993], '(pRS316),': [1994], 'resulting': [1995], 'centromeric': [1997], 'amino-terminal': [2001], 'MYC-tagged': [2002], 'driven': [2006], 'CUP1': [2009], 'copper-inducible': [2010], 'promoter.': [2011], 'These': [2012, 2193], 'pRSAtIPK2α': [2022], 'pRSAtIPK2β': [2024, 3054], 'S.': [2031, 3045, 3614, 3849, 3904], 'mutants': [2035], 'identified': [2036, 2922], 'Table': [2038], 'I.': [2039], 'mutagenesis': [2041], 'point': [2047, 2165, 2282, 4158], 'mutation': [2048, 2089, 2236], 'conserved': [2051, 3450, 3886], 'DXKXG': [2052], 'motif': [2053, 3447, 3460, 3667, 3781, 3890], 'kinases.': [2056, 3458, 3863], 'Mutations': [2057], 'region': [2060, 2108], 'shown': [2063, 3473, 4151], 'ablate': [2065], 'D98A': [2094], 'forAtIPK2β': [2096], 'D100A.': [2098], 'Complementary': [2099], 'designed': [2103, 3328], 'anneal': [2105], 'mutated': [2111, 2315, 2351], 'except': [2118, 2589, 2605], 'single': [2121, 2281], 'nucleotide': [2122, 2160], 'change': [2123], 'aspartic': [2126], 'acid': [2127], 'codon': [2128], 'changing': [2129], 'alanine': [2133], 'codon.': [2134], 'forAtIPK2α': [2137], 'follows:': [2140, 2173], '5′-TCA': [2142], 'GCT': [2145, 2178, 3426, 3428], 'ATG-3′': [2148], '5′-AGT': [2151], 'TAC': [2153, 2187], 'TTC': [2156, 2190, 3416, 3439], 'TAC-3′': [2157], 'where': [2158], 'italics': [2162], 'identifies': [2163], 'mutation.': [2166, 2283], '5′-CG': [2175], 'ATT': [2181, 3431], '5′-GC': [2185], 'TAA': [2191], 'C-3′.': [2192], 'combination': [2198], 'amplify': [2204, 3330], 'full': [2206], 'open': [2207, 2264, 3333], 'reading': [2208, 2265, 3334], 'frames': [2209, 3335], 'above.': [2211, 2720, 3044], 'reactions': [2214, 2233, 2769, 2877, 3037, 3235, 3250, 3285, 3352, 4189], 'Turbo': [2218], 'Pfu': [2219], 'fidelity': [2221], 'polymerase': [2222, 3270], '(Stratagene)': [2223], 'conditions': [2226, 2717, 3302], 'manufacturer.': [2230], 'Two': [2231], 'gel': [2242], 'subsequent': [2248, 3182, 3248], 'reaction': [2250, 2677, 2779, 2840, 2995, 3026, 3206, 3218, 3271, 3954, 4085, 4206], 'ends': [2260], 'full-length': [2263, 2277], 'frame': [2266], 'result': [2271, 3984], 'digested': [2288], 'NcoI': [2290], 'SalI,': [2292], 'analyzed': [2299, 2785, 2881, 3039, 3925], 'correct': [2301], 'sequence.': [2302], 'To': [2303, 2615, 2689, 2792, 2933, 4103, 4440], 'mutations': [2307], 'rendered': [2308], 'enzyme': [2310, 4386], 'inactive,': [2311], 'GST': [2320, 2551, 3936], 'fusion': [2321, 2552, 3937, 3965], 'bacteria': [2324], 'assayed': [2328], 'below': [2336], 'assays': [2338, 2722], '[3H]I(1,4,5)P3': [2340, 3970], 'substrate.': [2343], '100': [2344, 2622, 2684, 2696, 2805, 3068], 'ng': [2345, 2623, 2661, 2697, 2727, 2806, 2860, 3224], 'completely': [2355, 4192], 'unable': [2356], 'vitro(data': [2361], 'shown).': [2363, 4299], 'AtIPK2α-': [2365], 'andAtIPK2β-pGEX-KG': [2366], 'DH5α': [2374, 2390], 'E.': [2375, 3598], 'coli': [2376], 'cells.': [2377], 'liter': [2379], 'LB': [2381], 'inoculated': [2383], '20-ml': [2386], 'overnight': [2387], 'culture': [2388], 'cells': [2391, 2424, 2452, 3047, 3080], 'AtIPK2α-pGEX-KG': [2394], 'orAtIPK2β-pGEX-KG': [2395], 'plasmid': [2396], '37': [2400, 2431, 2674, 2751, 2823, 2874, 2991, 3031, 3199], '°C': [2401, 2533, 2577, 2685, 2752, 2824, 3032, 3177, 3200, 3243, 3290, 3309, 3314, 3320], 'absorbance': [2404], '0.6': [2406, 3276], '600': [2408], 'nm.': [2409], 'Expression': [2410], 'induced': [2412], '0.5': [2417, 2649], 'mm': [2418, 2493, 2564, 2569, 2633, 2638, 2641, 2644, 2647, 2757, 2764, 2902, 2976, 3006, 3018, 3022, 3221, 3277], 'isopropyl-β-d-thiogalactopyranoside': [2419], '(IPTG)': [2420], '(final': [2421], 'concentration).': [2422], '4': [2428, 2437, 2511, 3294], 'h': [2429, 3203], '°C,': [2432, 2438, 2457], 'harvested': [2433, 3082, 3121], 'centrifugation': [2435, 2502], 'resuspended': [2440], '20': [2442], 'ml': [2443, 2520, 2544, 2561, 3059], 'ice-cold': [2445, 2546], 'phosphate-buffered': [2446, 2547], 'saline': [2447], 'protease': [2449], 'inhibitors.': [2450], 'frozen': [2454], '−20': [2456], 'thawed': [2458], 'ice,': [2460], 'lysed': [2462], 'passages': [2465], 'cell': [2468, 2477], 'cracker': [2469], '(a': [2470], 'shear': [2472], 'fluid': [2473], 'processing': [2474], 'system': [2475], 'rupture,': [2478], 'Microfluidics': [2479], 'Corp.).': [2480], 'Phenylmethylsulfonyl': [2481], 'fluoride': [2482], 'Triton': [2484], 'X-100': [2485], 'added': [2487, 3011], 'final': [2489, 3014], 'concentrations': [2490, 2982], '1': [2492, 2519, 2560, 2687, 2756, 3017, 3058, 3193, 3202, 3220, 3322], '1%,': [2495], 'lysate': [2498, 3086], 'subjected': [2500], '10,000': [2504], '×': [2505, 2893], 'g': [2506], 'min': [2509, 2530, 2672, 2821, 2872, 2916, 2989, 3180, 3295, 4149], '°C.': [2512, 2675, 2875, 2992], 'supernatant': [2514], 'incubated': [2517, 2694, 2735, 2812, 2857, 2956, 3029, 3287, 3968, 4135], '50%': [2523], 'glutathione-Sepharose': [2524], 'slurry': [2525], 'Biosciences)': [2527], 'shaking.': [2535], 'Sepharose': [2537, 2558], 'washed': [2539], 'three': [2540, 3940], 'times': [2541, 2749, 2777, 3300], '50': [2543, 2632, 2637, 2759, 2763, 2859, 2971, 2975], 'saline,': [2548], 'eluted': [2555], '10': [2563, 2643, 2766, 2782, 2814, 2820, 2850, 2871, 2901, 2958, 2978, 3005, 3245, 3311, 4148], 'reduced': [2565], '30': [2568, 2776, 2988, 3034, 3188, 3299, 3316], 'Tris,': [2570], 'pH': [2571, 2635, 2761, 2973], '7.5': [2572], '−80': [2576], 'aliquots.': [2579], 'All': [2580], 'unlabeled': [2581, 4376], 'purchased': [2584, 2594, 2600], 'Cell': [2586, 3655], 'Signals,': [2587], 'Inc.': [2588], 'I(1,3,4,6)P4,': [2591, 2966, 4356], 'Inositols.com.': [2596], 'Tritiated': [2597], 'PerkinElmer': [2602], 'Life': [2603], 'Sciences': [2604], '[3H]I(1,3,4,6)P4': [2607, 2692, 2746], '[3H]I(1,2,3,4,6)P5,': [2609, 2691], 'follows.': [2614], 'synthesize': [2616, 2690], '[3H]I(1,3,4,6)P4,': [2617], '[3H]I(1,3,4)P3': [2618], 'mammalian': [2626, 4077], 'I(1,3,4)P3': [2627, 3729, 4213], '5/6-kinase': [2628], 'buffer': [2630, 2754, 3272], 'Hepes,': [2634], '7.5,': [2636, 2762, 2974], 'KCl,': [2639], '2': [2640, 4154], 'ATP,': [2642, 2758, 3972], 'MgCl2,': [2645], '14': [2646], 'phosphocreatine,': [2648], 'units': [2650, 3210, 3230, 3281], 'phosphocreatine': [2652], 'kinase,': [2653], '0.1': [2654], 'mg/ml': [2655], 'bovine': [2656], 'serum': [2657], 'albumin,': [2658], '150': [2660], '(to': [2666], 'avoid': [2667], 'I(1,3,4,5)P4': [2668, 2802, 4082, 4124], 'synthesis)': [2669], '60': [2671, 3313], 'terminated': [2679, 2842, 2879], 'heat': [2681, 3239], 'inactivation': [2682, 3240], 'min.': [2688, 3035, 3189, 3246, 3323], '2J.': [2702], 'Stevenson-Paulik,': [2703], 'Frye,': [2706], 'York,': [2710], 'manuscript': [2711], 'preparation.': [2713], 'same': [2716], 'tritiated': [2724], 'substrates,': [2725], '50–100': [2726], 'GST-AtIpk2α': [2731, 2808], 'GST-AtIpk2β': [2733, 2810], 'independently': [2736], '0.25–20': [2738], 'μm': [2739, 2959, 3069, 3259], '[3H]inositol,': [2740], '[3H]I(1)P,': [2741], '[3H]I(1,4)P2,': [2742], '[3H]I(1,4,5)P3,': [2743], '[3H]I(1,3,4,5)P4,': [2744], 'mmHepes': [2760, 2972], 'NaCl,': [2765, 2977], 'mmMgCl2.': [2767], '10-μl': [2768, 2994], 'stopped': [2771, 3237, 4139], 'volume': [2780], 'mmNH4H2PO4': [2783], 'Partisphere': [2787, 2886], 'strong': [2788, 2888], 'anion': [2789, 2889], 'exchange': [2790, 2890], 'HPLC.': [2791], 'determine': [2793], 'whether': [2794], 'I(1,4,5,6)P4,': [2804, 2963, 4354], 'μm[3H]I(1,4,5)P3': [2815], 'above': [2818, 3357], 'so': [2825], 'majority': [2828], 'IP5.': [2836], 'Half': [2837], '300': [2847, 3003], 'μl': [2848, 3004], 'mmNH4H2PO4,': [2851], 'half': [2855], 'type': [2864, 4109, 4160], 'I': [2865, 3159, 4110, 4161], 'another': [2870], 'HPLC': [2883, 3041], 'Sax': [2887], 'column': [2891], '(4.6': [2892], '125': [2894], 'mm)': [2895], 'linear': [2898], 'gradient': [2899], '1.7': [2904, 2918], 'mNH4H2PO4': [2905], '(pH': [2906], '3.5)': [2907], '12': [2909], 'min,': [2910], 'followed': [2911], 'elution': [2913, 2928], 'mNH4H2PO4.': [2919], 'based': [2923], 'comparison': [2925, 3810], 'species.': [2932], 'test': [2934, 3346, 4104], 'if': [2935], 'range': [2941], 'different': [2943, 4340], 'IPs,': [2944], '1.4': [2945], 'μg': [2946, 3194], 'GST-': [2949, 2952], 'individually': [2955, 4328], 'I(1,3,4)P3,': [2961], 'I(1,2,6)P3,': [2962], 'I(3,4,5,6)P4,': [2965], 'I(1,3,4,5,6)P5,': [2967], 'mmMgCl2,': [2979], 'trace': [2981, 4383], '[γ-32P]ATP': [2984], '(200,000': [2985], 'cpm)': [2986], 'quenched': [2998], 'NH4H2PO4,': [3007], 'MgATP': [3009], 'ATP': [3019, 4377], 'MgCl2': [3023], 'again': [3028], 'Quenched': [3036], 'pRS316': [3050], 'alone,': [3051], 'pRSAtIPK2α,': [3052], 'CuSO4': [3070], '40': [3072, 3229], 'μCi': [3073], '[3H]inositol': [3074], 'late': [3076], 'logarithmic': [3077], 'phase.': [3078], 'soluble': [3085], 'isolated': [3091, 3140], 'according': [3092, 3141], 'et': [3095], 'al.': [3096], 'Tissue': [3116], 'RNA': [3118, 3138, 3153, 3174, 3196, 3361], 'isolation': [3119], 'leaves,': [3124], 'stems,': [3125], 'roots,': [3126], 'siliques,': [3127], 'flowers': [3129], '3–4-week-old': [3131], '(ecotype': [3135], 'Columbia).': [3136], 'Total': [3137], "manufacturer's": [3144], 'recommendations': [3145], 'RNeasy': [3148], 'Mini': [3150], 'Kit': [3151], '(Qiagen).': [3152], 'samples': [3154], 'treated': [3156], 'DNase': [3158], 'remove': [3161, 4118], 'contaminating': [3163, 3348], 'genomic': [3164, 3349], 'DNA.': [3165], 'An': [3166], 'oligo(dT)': [3167, 3226], '(5′-T27VN-3′)': [3169], 'annealed': [3171], '70': [3176, 3242], 'cooling': [3183], 'room': [3185], 'temperature': [3186], 'Reverse': [3190], 'transcription': [3191], 'occurred': [3197], 'mix': [3207], '200': [3209], 'MMLV': [3212, 3217], 'reverse': [3213], 'transcriptase': [3214], '(Promega),': [3215], '1×': [3216], 'buffer,': [3219], 'dNTPs,': [3222, 3278], '500': [3223], 'primer,': [3227], 'RNase': [3232], 'inhibitor.': [3233], 'one-fourth': [3252], 'first': [3255], 'strand': [3256], '12.5': [3258], 'gene,': [3268], '1×Taq': [3269], 'Biochemicals),': [3275], '0.25': [3280], 'ofTaq': [3282], 'polymerase.': [3283], '94': [3289, 3308], 'initial': [3293, 4380], 'cycled': [3298], '(non-saturating': [3301], 'target': [3304], 'amplification)': [3306], 's,': [3312, 3317], '72': [3319], 'entire': [3332], 'AtIPK2α,AtIPK2β,': [3337], 'ACT2': [3339], 'U41998).': [3341], 'control': [3344, 4205], 'DNA,': [3350], 'samples.': [3362], '5′-TGC': [3368, 3396], 'TCA': [3370, 3383, 3397], 'TCC': [3372, 3399], 'AAC': [3374, 3401], 'ATC': [3375, 3414], 'AGG': [3376, 3398], 'TT-3′': [3377], '(sense)': [3378, 3406, 3434], '5′-TCT': [3380], 'GCA': [3381], 'GAC': [3382, 3441], 'AGA': [3388], 'AT-3′': [3389, 3417], '(antisense).': [3390, 3418, 3445], 'TTG': [3404], 'CT-3′': [3405], '5′-GTT': [3408], 'TAG': [3411], 'GGA': [3412], 'GGT': [3415], 'amplifyACT2': [3423], '5′-ATG': [3425], 'GAG': [3427], 'GAT': [3429, 3430], 'C-3′': [3433, 3444], '5′-TGT': [3436], 'PxxxDxKxG': [3448, 3894], 'among': [3451], 'growing': [3453], 'family': [3454, 3947], 'This': [3459, 3983], 'originally': [3462], 'common': [3465], '3-kinases,': [3468, 3490], 'subsequently': [3470], 'several': [3475], 'research': [3476], 'groups': [3477], 'hallmark': [3481], 'family,': [3485, 3879, 4312], 'whose': [3486], 'members': [3487, 3875, 4307], 'include': [3488], 'IP3/IP4dual-specificity': [3491], '6-/3-kinases': [3492], '(also': [3493, 3503], 'Ipmk),': [3498], 'synthases': [3502], 'IP6K)': [3506], '(5Saiardi': [3507], '22Schell': [3548], 'Brearley': [3552], 'Biber': [3554], 'Murer': [3556], '461:': [3563], '169-172Crossref': [3564], '(74)': [3567, 3764], '23Saiardi': [3570], 'Erdjument-Bromage': [3572], 'Snowman': [3574, 3605], 'A.M.': [3575, 3606], 'Tempst': [3576], '9:': [3583], '1323-1326Abstract': [3584], '(310)': [3592], '24Saiardi': [3595], 'Nagata': [3597], 'Luo': [3599, 3603], 'H.R.': [3600], 'Sawa': [3601], 'X.': [3604], 'Proc.': [3609], 'Natl.': [3610], 'Acad.': [3611], 'U.': [3613], '98:': [3617], '2306-2311Crossref': [3618], '(95)': [3621], '25Choi': [3624], 'K.Y.': [3625], 'Kim': [3626, 3634], 'H.K.': [3627, 3637], 'Lee': [3628], 'S.Y.': [3629], 'Moon': [3630], 'K.H.': [3631], 'Sim': [3632], 'S.S.': [3633], 'J.W.': [3635], 'Chung': [3636], 'Rhee': [3638], 'S.G.': [3639], '248:': [3642], '64-66Crossref': [3643], '(116)': [3646], '26Communi': [3649], 'Vanweyenberg': [3651], 'Erneux': [3653, 3909], 'C.': [3654, 3910, 4058], 'Signal.': [3656], '1995;': [3657], '7:': [3658], '643-650Crossref': [3659], '(40)': [3662], 'Interestingly,': [3665], 'axial': [3677], 'D-2': [3678], 'position': [3679, 4121, 4432], 'ring': [3683], '27Ives': [3703], 'Nichols': [3705], '36575-36583Abstract': [3716], '(69)': [3724], 'nor': [3727], '5/6-kinases': [3730], '28Wilson': [3747], '271:': [3755], '11904-11910Abstract': [3756], 'order': [3768], 'identify': [3770], 'orthologs,': [3772], 'pattern': [3774], 'searched': [3775], 'genome': [3778], 'PatMatch': [3784], 'program': [3785], 'TAIRArabidopsis': [3789], 'data': [3790], 'base.': [3791], 'We': [3792, 4217], 'candidate': [3795], 'sequences,': [3796], 'GenBankTM': [3797], 'accession': [3798], 'AJ245521': [3801, 3841, 3933, 4030], 'PsvmDvKmG': [3805], 'PsvmDvKiG': [3807], 'Pair-wise': [3809], 'putative': [3814, 3899], 'reveals': [3817], '78%': [3824], 'structure.': [3834], 'predicted': [3836], '25–28%': [3846], 'rat': [3853, 3869, 4005], 'multikinase': [3856], '(rIpmk),': [3857], 'plant,': [3866], 'aligned': [3872], 'five': [3880], 'motifs': [3881], 'emerge': [3882], 'highly': [3885], '(Fig.1).': [3887], 'second': [3889], 'contains': [3891], 'canonical': [3893], 'binding': [3901], '(29Togashi': [3903], 'Takazawa': [3905], 'Endo': [3907], 'T.': [3908, 3912], 'Onaya': [3911], '326:': [3916], '221-225Crossref': [3917], '(31)': [3920], 'Next': [3923], 'selectivity': [3928], 'recombinantAJ404678': [3931], 'proteins.': [3938], 'types': [3941], 'distinguishable': [3949], 'analysis': [3951], 'substrates': [3958], 'intermediate': [3980, 4095, 4143], '(Fig.': [3981, 4214, 4394, 4406], '2).': [3982], 'fall': [3990], 'analogous': [3999], 'theS.': [4001], 'Ipmk.': [4006], 'tested': [4008, 4315], '(or': [4014], 'IP5)': [4015], 'neither': [4016], 'produced': [4019], 'products.': [4023], 'Therefore,': [4024], 'AtIpk2β,': [4034, 4285], 'Others': [4036], '(18Saiardi': [4037, 4254], '30Nalaskowski': [4055], 'M.M.': [4056], 'Deschermeier': [4057], 'Fanick': [4059], 'W.': [4060], 'Mayr': [4061], 'G.W.': [4062], '2002;': [4065], '366:': [4066], '549-556Crossref': [4067], '(83)': [4070], 'reported': [4074], 'product.': [4086], 'Thus,': [4087], 'determined': [4089], 'nature': [4091], '5-phosphatase,': [4113], 'D-5': [4120, 4431], 'phosphate': [4122], 'I(1,4,5,6)P4.': [4129], 'Recombinant': [4130], 'when': [4140], 'maximal': [4145], '(time': [4146], 'Fig.': [4153], 'B)': [4155], 'added.': [4164], 'seen': [4166], 'Fig.3': [4168], 'A,': [4169], 'treatment': [4171], 'hydrolyze': [4174], 'isomer(s)': [4176], 'AtIpk2β.': [4182], 'active': [4186], 'because': [4190], 'dephosphorylated': [4193], 'remaining': [4195], 'I(1,4)P2': [4199], '(Fig.3': [4200], 'A),': [4201], 'readily': [4208], '[3H]I(1,3,4,5)P4': [4210], '3': [4215], 'B).': [4216], 'published': [4219], 'Given': [4247], 'retested': [4273], 'GST-Ipk2': [4275], 'experiments': [4278], 'that,': [4281], 'like': [4282], 'synthesizes': [4288], 'major': [4292], '(data': [4297], 'Multiple': [4300], 'therefore,': [4313], 'panel': [4317, 4337], 'available': [4319], 'possible': [4322], 'substrates.': [4323], 'testedin': [4329], 'fourteen': [4339], '"Experimental': [4346], 'Procedures."': [4347], 'Detectable': [4348], 'observed': [4351], 'I(1,2,3,4,6)P5.': [4357], 'expected,': [4359], '(Fig.4,': [4367], 'A': [4368], 'C)': [4370], 'after': [4378], 'incubation': [4381], '[γ-32P]ATP,': [4384], 'additionally': [4387], '4,': [4395, 4414], 'B': [4396], 'D).': [4398], '4,E': [4407], 'G)': [4409], 'well': [4411], 'I(1,3,4,5)P4(Fig.': [4413], 'F': [4415], 'H).': [4417], 'Surprisingly,': [4418], 'able': [4423], '(1,2,3,4,6)P5': [4428], 'respectively': [4438], '(Fig.5).': [4439], 'knowledge,': [4442], 'mem': [4453]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2021198651', 'counts_by_year': [{'year': 2024, 'cited_by_count': 7}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 7}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 8}, {'year': 2017, 'cited_by_count': 9}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 5}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 5}, {'year': 2012, 'cited_by_count': 7}], 'updated_date': '2025-01-02T02:24:12.696830', 'created_date': '2016-06-24'}
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