Title: Effect of chloramphenicol on its biosynthesis by <i>Streptomyces</i> species 3022<i>a</i>
Abstract: Measurements of ( 3 H) chloramphenicol production in cultures of Streptomyces species 3022a grown on a medium containing (6- 3 H) D-glucose and (3- 14 C) chloramphenicol showed that chloramphenicol inhibits its own biosynthesis. Similar results were obtained in cultures supplemented with the antibacterial p-methylthio- analogue of chloramphenicol. Here synthesis of the antibiotic was completely suppressed until the concentration of analogue had been reduced by inactivating enzymes. In contrast, the L-threo- and p-methylsulfonyl- analogues did not delay growth of the organism and had little effect on chloramphenicol biosynthesis. However, like chloramphenicol and its p-methylthio- analogue, the L-threo and p-methylsulfonyl compounds were degraded. Degradation of chloramphenicol and the p-methylsulfonyl-analogue ceased when endogenously produced antibiotic reached a concentration of 10–30 mg/liter, suggesting that changes in cell permeability are associated with the onset of chloramphenicol synthesis.
Publication Year: 1972
Publication Date: 1972-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 31
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