Title: Functional estrogen receptors in adult articular cartilage: Estrogen replacement therapy increases chondrocyte synthesis of proteoglycans and insulin‐like growth factor binding protein 2
Abstract: Arthritis & RheumatismVolume 43, Issue 9 p. 2081-2090 Basic ScienceFree to Read Functional estrogen receptors in adult articular cartilage: Estrogen replacement therapy increases chondrocyte synthesis of proteoglycans and insulin-like growth factor binding protein 2 Renee S. Richmond, Renee S. Richmond The Wake Forest University School of Medicine, Winston-Salem, North CarolinaSearch for more papers by this authorCathy S. Carlson, Cathy S. Carlson University of Minnesota, St. PaulSearch for more papers by this authorThomas C. Register, Thomas C. Register The Wake Forest University School of Medicine, Winston-Salem, North CarolinaSearch for more papers by this authorGouri Shanker, Gouri Shanker Rush Medical College of Rush–Presbyterian–St. Luke's Medical Center, Chicago, IllinoisSearch for more papers by this authorRichard F. Loeser, Corresponding Author Richard F. Loeser Rush Medical College of Rush–Presbyterian–St. Luke's Medical Center, Chicago, IllinoisRheumatology, Rush–Presbyterian–St. Luke's Medical Center, 1725 West Harrison Street, Suite 1017, Chicago, IL 60612-3824Search for more papers by this author Renee S. Richmond, Renee S. Richmond The Wake Forest University School of Medicine, Winston-Salem, North CarolinaSearch for more papers by this authorCathy S. Carlson, Cathy S. Carlson University of Minnesota, St. PaulSearch for more papers by this authorThomas C. Register, Thomas C. Register The Wake Forest University School of Medicine, Winston-Salem, North CarolinaSearch for more papers by this authorGouri Shanker, Gouri Shanker Rush Medical College of Rush–Presbyterian–St. Luke's Medical Center, Chicago, IllinoisSearch for more papers by this authorRichard F. Loeser, Corresponding Author Richard F. Loeser Rush Medical College of Rush–Presbyterian–St. Luke's Medical Center, Chicago, IllinoisRheumatology, Rush–Presbyterian–St. Luke's Medical Center, 1725 West Harrison Street, Suite 1017, Chicago, IL 60612-3824Search for more papers by this author First published: 26 March 2001 https://doi.org/10.1002/1529-0131(200009)43:9<2081::AID-ANR20>3.0.CO;2-ICitations: 86AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Objective Epidemiologic studies suggest a protective effect of estrogen replacement therapy (ERT) against the development of knee and hip osteoarthritis, but a potential mechanism for this effect is not known. The present study was done to determine if functional estrogen receptors (ERs) are present in adult articular cartilage and to determine if ERT in vivo affects the production of insulin-like growth factor binding proteins (IGFBPs). Methods Reverse transcription–polymerase chain reaction, immunoblotting, and immunohistochemistry were used to measure messenger RNA (mRNA) and protein for ERs in adult monkey articular cartilage. Cultured chondrocytes transfected with a reporter construct containing the estrogen response element (ERE/luciferase) were stimulated with estrogen in vitro to determine functional activity of the ERs. IGFBP production was measured by ligand and immunoblotting of conditioned media of cells cultured from control and estrogen-treated surgically menopausal monkeys. Proteoglycan (PG) synthesis was estimated by measurement of 35SO4 incorporation. Results ERα and ERβ mRNA were present in adult monkey articular cartilage, and ER protein was demonstrated by immunoblotting and immunohistochemistry. Estrogen treatment in vitro of cells transfected with the ERE/luciferase construct resulted in a 2.87-fold increase (P = 0.0163) in reporter production over that of untreated cells. Compared with untreated controls, IGFBP-2 production was significantly increased (P < 0.008) in conditioned media of chondrocytes cultured from monkeys that had received ERT in vivo. Increased IGFBP-2 in these cultures was associated with a 1.41-fold increase (P = 0.02) in the level of sulfate incorporation. Conclusion Transcriptionally functional ER are present in adult articular cartilage, and ERT increases the production of IGFBP-2 and the synthesis of PGs by chondrocytes from surgically menopausal monkeys. These results indicate that estrogen can have a direct effect on adult articular cartilage. Citing Literature Volume43, Issue9September 2000Pages 2081-2090 RelatedInformation
Publication Year: 2000
Publication Date: 2000-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 143
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot