Publication Information

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469, 594, 3242, 3316], 'protein,': [24, 208], 'extracellular': [26, 111, 143, 210, 295, 327, 472, 604, 630, 687, 3411], 'concentration': [27, 113, 145, 211, 297, 329, 592, 666, 2611, 3027, 3208, 3606, 3778, 3798, 4023, 4396], 'Aβ': [29, 71, 112, 123, 144, 213, 255, 296, 307, 328, 447, 506, 547, 549, 569, 595, 618, 651, 665, 688, 897, 2487, 2505, 2508, 2558, 2610, 3985, 4022, 4035, 4058, 4076, 4100, 4409], 'determined': [31, 215, 2613, 3029, 3696, 4265, 4297, 4398, 4431, 4462], 'not': [32, 216, 510, 1236, 2055, 2113, 2457, 2471, 3603, 4064], 'only': [33, 217, 511, 1325], 'by': [34, 39, 146, 157, 218, 223, 330, 341, 431, 600, 603, 607, 613, 667, 1072, 1332, 1714, 2081, 2511, 2614, 2635, 2644, 2794, 2797, 2969, 2996, 3030, 3127, 3140, 3235, 3244, 3267, 3322, 3335, 3360, 3408, 3452, 3477, 3480, 3495, 3595, 3613, 3627, 3672, 3685, 3730, 3739, 3781, 3809, 3915, 4015, 4077, 4106, 4252, 4316, 4379, 4387, 4399, 4450], 'its': [35, 40, 219, 224, 513, 522, 669, 1832], 'production': [36, 220, 514, 539], 'but': [37, 221, 515, 1503], 'also': [38, 222, 516, 889, 1141, 1633, 1893], 'catabolism.': [41, 225, 548, 2506], 'A': [42, 226, 1223, 3348, 3487], 'major': [43, 227], 'focus': [44, 228], 'research': [47, 231], 'has': [48, 232, 527, 1051, 1235, 1287, 1938, 2075, 2188, 2404, 2456, 2470, 4060], 'been': [49, 233, 528, 890, 1052, 1122, 1237, 1288, 1634, 1940, 2076, 2114, 2406, 2459, 2472, 4061], 'elucidation': [51, 235], 'mechanisms': [54, 68, 238, 252, 519], 'responsible': [55, 69, 239, 253, 520, 1830, 2452], 'for': [56, 70, 240, 254, 521, 589, 642, 676, 1003, 1831, 2416, 2453, 2500, 2618, 2623, 2762, 3074, 3175, 3231, 3241, 3450, 3523, 3735, 3828, 3859, 4006, 4164, 4332, 4440, 4446, 4456], 'generation': [58, 242, 570], 'Aβ.': [60, 244], 'Much': [61, 245], 'less,': [62, 246], 'however,': [63, 247, 1241], 'known': [65, 249, 545], 'about': [66, 250], 'removal': [72, 256], 'brain.': [75, 259], 'In': [76, 260, 969, 1043, 1283, 1701, 1835, 2008, 2296, 4305], 'this': [77, 152, 261, 336, 635, 970, 1046, 1752, 2177, 2454, 2501, 3625, 3782, 3785, 3974, 4068], 'report,': [78, 262, 971], 'we': [79, 126, 165, 263, 310, 349, 972, 2476, 4318], 'describe': [80, 264, 973], 'identification': [82, 266, 646, 975], 'endothelin-converting': [84, 268, 371, 977, 1119], 'enzyme-1': [85, 269, 978], '(ECE-1)': [86, 270, 979], 'as': [87, 271, 980, 1852, 1854, 2514, 2994, 3238, 3593, 3697, 3845, 4083, 4157], 'a': [88, 107, 272, 291, 639, 674, 894, 981, 990, 1008, 1020, 1333, 1819, 2062, 2082, 2216, 2497, 2645, 2913, 3351, 3440, 3541, 3816, 3983, 4028, 4093, 4110, 4115, 4146, 4188, 4204, 4210, 4267, 4422], 'novel': [89, 273, 660, 982], 'Aβ-degrading': [90, 274, 983], 'enzyme.': [91, 275, 984], 'We': [92, 276, 4429], 'show': [93, 127, 166, 277, 311, 350], 'that': [94, 114, 128, 151, 167, 278, 298, 312, 335, 351, 649, 1763, 1818, 1995, 2053, 2070, 2155, 2256, 2463, 2483, 3358, 3792, 3804, 3815, 4059, 4445], 'treatment': [95, 158, 279, 342, 3156], 'endogenous': [97, 139, 281, 323, 1837, 2211], 'ECE-expressing': [98, 282], 'cell': [99, 283, 394, 1256, 1841, 1857, 2654, 2807, 2817, 2843, 3491], 'lines': [100, 284, 2818, 2966], 'metalloprotease': [103, 287], 'inhibitor': [104, 288, 3868], 'phosphoramidon': [105, 289, 3121, 3185, 3927, 4380], 'causes': [106, 290], '2–3-fold': [108, 292], 'elevation': [109, 293], 'appears': [115, 299, 1705], 'to': [116, 119, 148, 300, 303, 332, 537, 553, 657, 892, 1006, 1023, 1045, 1054, 1571, 1636, 1640, 1706, 1889, 1933, 1942, 1946, 1992, 2069, 2174, 2551, 2748, 2903, 2910, 2959, 3155, 3265, 3285, 3296, 3314, 3345, 3357, 3465, 3558, 3589, 3769, 3872, 3942, 3978, 4041, 4308, 4418, 4444], 'be': [117, 301, 597, 638, 1637, 1707, 1943, 1990, 2911, 3590, 3979, 4065], 'due': [118, 302, 1991], 'inhibition': [120, 304], 'intracellular': [122, 306, 560, 1646, 1770, 1833, 1849, 2228], 'degradation.': [124, 308, 615], 'Furthermore,': [125, 309], 'overexpression': [129, 313], 'ECE-1': [131, 170, 315, 354, 396, 1050, 1244, 1285, 1323, 1844, 2086, 2484, 2674, 2679, 2685, 3355], 'Chinese': [133, 317, 373], 'hamster': [134, 318, 374], 'ovary': [135, 319, 375], 'cells,': [136, 320, 3270], 'which': [137, 321, 1887, 2745, 2906, 3259, 3438, 4033], 'lack': [138, 322], 'ECE': [140, 324, 1228, 2401, 2464, 2907, 3064, 3867], 'activity,': [141, 325, 3065], 'reduces': [142, 326], 'up': [147, 331], '90%': [149, 333], 'effect': [153, 337], 'completely': [155, 339], 'reversed': [156, 340], 'cells': [161, 345, 1135, 1146, 1292, 1747, 1778, 2562, 2693, 2855, 2877, 2901, 2949, 2972, 2982, 3199, 3275, 3305, 3454, 3501, 3511, 3842, 3995], 'phosphoramidon.': [163, 347], 'Finally,': [164, 348], 'recombinant': [168, 352], 'soluble': [169, 353, 395, 3352], 'capable': [172, 356], 'hydrolyzing': [174, 358], 'synthetic': [175, 359, 2624, 3945, 4160, 4325], 'Aβ40': [176, 360, 2553, 2625, 3232, 3946, 4086, 4161, 4314, 4326, 4395, 4441, 4472], 'Aβ42': [178, 362, 2627, 3234, 3948], 'vitro': [180, 364, 2490], 'at': [181, 365, 530, 556, 565, 620, 663, 1019, 1855, 2773, 3077, 3160, 3194, 3222, 3461, 3640, 3862, 3921, 4003, 4050, 4167, 4241, 4337, 4463], 'multiple': [182, 366, 557], 'sites.': [183, 367, 2786], 'disease': [369, 415, 2469], 'enzyme': [372, 398, 679, 2064, 2451, 2502, 3773, 3777, 4080, 4321, 4371], 'amyloid': [376, 383], 'precursor': [377, 380, 384, 454], 'protein': [378, 381, 385, 455, 3449, 3570, 3797], 'neprilysin': [386, 681], 'endothelin': [387, 986], 'trans-Golgi': [388, 1756], 'network': [389, 1757], 'human': [390, 1233, 1243, 1322, 1703, 1744, 1764, 1890, 1934, 1985, 2058, 2109, 2151, 2168, 2181, 2650], 'umbilical': [391, 2651], 'vein': [392, 2652], 'endothelial': [393, 1134, 2653, 2842], 'angiotensin-converting': [397], 'enzyme-linked': [399], 'immunosorbent': [400], 'assay': [401, 3032], 'phenylmethylsulfonyl': [402], 'fluoride': [403, 3103], 'N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine': [404], '4-morpholineethanesulfonic': [405], 'acid': [406, 4129, 4137], 'high': [407], 'pressure': [408], 'liquid': [409], 'chromatography': [410], '3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,': [411], 'inner': [412], 'salt': [413, 3170, 3518], '(AD)1': [416], 'most': [419, 2259, 2349], 'common': [420, 2747], 'cause': [421], 'dementia': [423], 'elderly': [426], 'characterized': [429], 'pathologically': [430], 'accumulation': [433, 507, 628], 'form': [443, 1024, 3353], 'senile': [445], 'plaques.': [446], 'normally': [449], 'produced': [450, 1331], 'from': [451, 2085, 2408, 2560, 2649, 2691, 2812, 2821, 3573, 3702, 3795, 3839, 3992, 4095], '(βAPP)': [456], 'through': [457, 1341, 3540], 'combined': [459], 'proteolytic': [460], 'actions': [461], 'β-': [463], 'γ-secretase': [465], 'then': [468, 3173, 3521, 3728, 4025], 'into': [470, 2779, 3149, 3435, 3499], 'milieu': [473], '(1Checler': [474], 'F.': [475, 1211, 1800, 3395], 'J.': [476, 712, 723, 755, 790, 802, 868, 907, 925, 953, 1086, 1101, 1196, 1212, 1275, 1309, 1379, 1458, 1484, 1530, 1556, 1592, 1618, 1666, 1691, 1734, 1786, 1808, 1876, 1907, 1914, 1956, 1963, 2020, 2027, 2091, 2197, 2285, 2332, 2373, 2386, 2437, 3753], 'Neurochem.': [477, 771, 1310], '1995;': [478, 806, 1382, 1405, 2094, 2200, 2288, 2389, 2602], '65:': [479], '1431-1444Crossref': [480], 'PubMed': [481, 498, 698, 734, 760, 776, 794, 809, 841, 876, 912, 936, 964, 1038, 1090, 1112, 1178, 1218, 1314, 1368, 1390, 1408, 1436, 1462, 1489, 1534, 1561, 1596, 1623, 1670, 1696, 1738, 1790, 1812, 1880, 1925, 1974, 2038, 2097, 2139, 2203, 2244, 2291, 2338, 2377, 2392, 2445, 2538, 2605, 2943, 3021, 3375, 3401, 3764, 3910], 'Scopus': [482, 499, 699, 735, 777, 795, 810, 842, 877, 937, 965, 1039, 1091, 1113, 1179, 1200, 1219, 1279, 1315, 1369, 1391, 1409, 1437, 1463, 1490, 1535, 1562, 1597, 1624, 1671, 1697, 1739, 1791, 1813, 1881, 1926, 1975, 2039, 2098, 2140, 2204, 2245, 2292, 2339, 2378, 2393, 2446, 2539, 2606, 2944, 3022, 3376, 3402, 3765, 3911], '(422)': [483], 'Google': [484, 501, 701, 737, 761, 779, 797, 812, 844, 879, 913, 939, 967, 1041, 1093, 1115, 1181, 1202, 1221, 1281, 1317, 1371, 1393, 1411, 1439, 1465, 1492, 1537, 1564, 1599, 1626, 1673, 1699, 1741, 1793, 1815, 1883, 1928, 1977, 2041, 2100, 2142, 2206, 2247, 2294, 2341, 2380, 2395, 2448, 2541, 2608, 2946, 3024, 3378, 3404, 3767, 3913], 'Scholar,': [485, 702, 738, 762, 780, 798, 813, 845, 914, 940, 1094, 1182, 1203, 1372, 1394, 1412, 1440, 1466, 1538, 1600, 2381], '2Golde': [486], 'T.E.': [487, 2531], 'Eckman': [488, 2528], 'C.B.': [489], 'Younkin': [490, 2532], 'S.G.': [491, 2533], 'Biochim.': [492], 'Biophys.': [493, 3371, 3904], 'Acta.': [494], '2000;': [495, 757, 773, 791, 838, 873, 2335], '1502:': [496], '172-187Crossref': [497], '(259)': [500], 'Scholar).': [502, 880, 968, 1042, 1116, 1222, 1282, 1318, 1493, 1565, 1627, 1700, 1884, 1929, 1978, 2101, 2207, 2248, 2295, 2342, 2396, 2449, 2609, 2947, 3025], 'The': [503, 591, 985, 1124, 1494, 2102, 2450, 2461, 2572, 2716, 2728, 2740, 2765, 2787, 3425, 3470, 3584, 3605, 3668, 3691, 3775, 3968, 4170, 4199, 4231, 4258, 4288, 4383, 4406, 4452], 'degree': [504], 'dependent': [509], 'on': [512, 517, 1074, 1337, 1743, 1896, 2167, 3273, 3287, 3303, 4109, 4175], 'removal.': [523, 670], 'While': [524], 'considerable': [525], 'effort': [526], 'directed': [529], 'elucidating': [531], 'enzymes': [533, 648, 988, 1120], 'pathways': [535], 'contributing': [536], 'Aβ,': [541, 631], 'much': [542], 'less': [543], 'regarding': [546], 'catabolism': [550, 636], 'likely': [552], 'involve': [554], 'proteases': [555, 605], 'sites,': [558], 'both': [559, 677, 1227, 2478, 2488, 2749], 'extracellular.': [562], 'Proteases': [563], 'acting': [564], 'site': [567, 1000, 2776], 'and/or': [571], 'within': [572, 1248], 'secretory': [574, 2233], 'pathway': [575, 2234], 'may': [576, 596, 637, 655, 1989, 2465, 2584], 'degrade': [577, 650], 'peptide': [579, 587, 3137, 3682, 4101, 4295], 'intracellularly,': [580], 'thus': [581, 2583], 'limiting': [582], 'amount': [584, 3969], 'available': [588], 'secretion.': [590], 'further': [598, 3611, 4071], 'regulated': [599], 'direct': [601], 'degradation': [602, 685, 898, 4074], 'receptor-mediated': [608], 'endocytosis': [609], 'or': [610, 2588, 3049, 3191, 3814, 3926, 3930, 3947, 3996, 4054, 4334, 4372], 'phagocytosis': [611], 'followed': [612, 3334], 'lysosomal': [614], 'Catabolism': [616], 'each': [621, 4294, 4459], 'these': [623, 4412], 'steps': [624], 'would': [625], 'limit': [626], 'disruption': [633], 'risk': [640], 'factor': [641], 'AD.': [643], 'Additionally,': [644], 'intracellularly': [652, 2186], 'extracellularly': [654], 'lead': [656], 'development': [658], 'therapeutics': [661], 'aimed': [662], 'reducing': [664], 'enhancing': [668], 'Recent': [671], 'reports': [672], 'suggest': [673], 'role': [675, 895, 2462, 2499], 'insulin-degrading': [678], '(NEP)': [682], '(3Kurochkin': [689], 'I.V.': [690], 'Goto': [691], 'S.': [692, 800, 817, 851, 853, 855, 1166, 1356, 2327, 2433, 2435, 2931, 3009, 3888, 3898], 'FEBS': [693, 1403, 1431, 2440, 3396], 'Lett.': [694, 1404, 1432, 2441, 3397], '1994;': [695, 1172, 1362, 1433, 2535, 2937, 3015], '345:': [696], '33-37Crossref': [697], '(346)': [700], '4Qiu': [703], 'W.Q.': [704, 744, 942], 'Walsh': [705, 745], 'D.M.': [706], 'Ye': [707, 741, 945], 'Z.': [708, 742, 946], 'Vekrellis': [709, 783], 'K.': [710, 740, 784, 821, 865, 1100, 1302, 1396, 1402, 2123, 2425, 3364, 3752, 3876], 'Zhang': [711], 'Podlisny': [713], 'M.B.': [714], 'Rosner': [715, 751, 785], 'M.R.': [716, 752, 786], 'Safavi': [717], 'A.': [718, 764, 1162, 1352, 1442, 1474, 1476, 1514, 1546, 1548, 1576, 1608, 1610, 1650, 1681, 1683, 1718, 1780, 1860, 2127, 2357, 2524, 2592, 2927, 3005], 'Hersh': [719], 'L.B.': [720], 'Selkoe': [721, 753, 787, 951], 'D.J.': [722, 754, 788, 952], 'Biol.': [724, 926, 954, 1102, 1380, 1915, 1964, 2028, 2092, 2198, 2286, 2387, 3754], 'Chem.': [725, 927, 955, 1103, 1381, 1916, 1965, 2029, 2093, 2199, 2287, 2388, 3755], '1998;': [726, 1197, 1276, 1787, 2136, 2442, 3372, 3907], '273:': [727], '32730-32738Abstract': [728], 'Full': [729, 731, 931, 933, 959, 961, 1107, 1109, 1175, 1365, 1385, 1387, 1920, 1922, 1969, 1971, 2033, 2035, 2940, 3018, 3759, 3761], 'Text': [730, 732, 932, 934, 960, 962, 1108, 1110, 1176, 1366, 1386, 1388, 1921, 1923, 1970, 1972, 2034, 2036, 2941, 3019, 3760, 3762], 'PDF': [733, 935, 963, 1111, 1177, 1367, 1389, 1924, 1973, 2037, 2942, 3020, 3763], '(708)': [736], '5Vekrellis': [739], 'Qiu': [743], 'D.': [746, 748, 1158, 1168, 1348, 1358, 1470, 1542, 1604, 1677, 2923, 2933, 3001, 3011, 3884], 'Hartley': [747], 'Chesneau': [749], 'V.': [750, 782], 'Neurosci.': [756, 908], '20:': [758], '1657-1665Crossref': [759], '6Perez': [763], 'Morelli': [765], 'L.': [766, 2527, 4098], 'Cresto': [767], 'J.C.': [768], 'Castano': [769], 'E.M.': [770], 'Res.': [772, 2135, 2240, 3905], '25:': [774], '247-255Crossref': [775], '(212)': [778], '7Chesneau': [781], 'Biochem.': [789, 869, 1033, 1085, 1457, 1485, 1529, 1557, 1591, 1619, 1665, 1692, 1733, 1785, 1807, 1875, 2372, 3370, 3903], '351:': [792], '509-516Crossref': [793], '(84)': [796], '8Howell': [799], 'Nalbantoglu': [801], 'Crine': [803, 1783, 1805], 'P.': [804, 1205, 1209, 1446, 1518, 1580, 1654, 1722, 1784, 1806, 1864, 2361, 3385, 3393], 'Peptides.': [805], '16:': [807, 910], '647-652Crossref': [808], '(186)': [811], '9Iwata': [814], 'N.': [815, 849, 1160, 1350, 1901, 1950, 2014, 2088, 2125, 2131, 2194, 2282, 2323, 2383, 2518, 2600, 2925, 3003, 3900], 'Tsubuki': [816, 850], 'Takaki': [818], 'Y.': [819, 833, 847, 1903, 1952, 2016, 2431, 2594], 'Watanabe': [820], 'Sekiguchi': [822], 'M.': [823, 827, 1170, 1360, 1398, 1400, 1414, 1911, 1913, 1960, 1962, 2024, 2026, 2090, 2196, 2284, 2331, 2385, 2935, 3013, 3902], 'Hosoki': [824], 'E.': [825, 831, 1376, 1418], 'Kawashima-Morishima': [826], 'Lee': [828, 862, 3885], 'H.J.': [829, 863], 'Hama': [830], 'Sekine-Aizawa': [832], 'Saido': [834, 866], 'T.C.': [835, 867, 1306], 'Nat.': [836], 'Med.': [837], '6:': [839], '143-150Crossref': [840], '(0)': [843], '10Takaki': [846], 'Iwata': [848], 'Taniguchi': [852], 'Toyoshima': [854], 'Lu': [856], 'B.': [857, 1416], 'Gerard': [858, 860], 'N.P.': [859], 'C.': [861, 948, 1164, 1188, 1267, 1354, 1480, 1552, 1614, 1687, 2529, 2598, 2929, 3007, 3389, 3886], 'Shirotani': [864], '(': [870], 'Tokyo': [871], ').': [872], '128:': [874], '897-902Crossref': [875], '(85)': [878], 'Matrix': [881], 'metalloproteinase-9,': [882], 'EC': [883], '3.4.24.15,': [884], 'α2-macroglobulin': [886], 'complexes': [887], 'have': [888, 1121, 1632, 2112], 'reported': [891, 1053, 1635, 2145], 'play': [893, 2466], '(11Backstrom': [899], 'J.R.': [900], 'Lim': [901], 'G.P.': [902], 'Cullen': [903], 'M.J.': [904], 'Tokes': [905], 'Z.A.': [906], '1996;': [909, 956, 1035], '7910-7919Crossref': [911], '12Yamin': [915], 'R.': [916, 1424, 1478, 1550, 1612, 1685], 'Malgeri': [917], 'E.G.': [918], 'Sloane': [919], 'J.A.': [920, 2321], 'McGraw': [921], 'W.T.': [922], 'Abraham': [923], 'C.R.': [924], '1999;': [928, 1104, 1215, 1486, 1558, 1620, 1693, 1809, 1917, 1966, 2030, 2241, 3756], '274:': [929, 1105, 1918, 1967, 2031, 3757], '18777-18784Abstract': [930], '(88)': [938], '13Qiu': [941], 'Borth': [943], 'W.': [944], 'Haass': [947], 'Teplow': [949], 'D.B.': [950], '271:': [957], '8443-8451Abstract': [958], '(93)': [966], 'converting': [987], 'are': [989, 1330, 2054], 'class': [991], 'type': [993], 'II': [994, 2706], 'integral': [995], 'membrane': [996, 1574, 1643, 1899, 2990, 3066], 'zinc': [997], 'metalloproteases': [998], '(active': [999], 'luminal)': [1001], 'named': [1002], 'their': [1004, 1507], 'ability': [1005], 'hydrolyze': [1007, 1055, 2486], 'family': [1009, 2503], 'biologically': [1011, 1057], 'inactive': [1012, 3824], 'intermediates,': [1013], 'big': [1014, 1498, 2347, 2354, 2420, 3083, 3620, 3634, 3736, 4447, 4476], 'endothelins': [1015, 1028], '(big': [1016], 'ETs),': [1017], 'exclusively': [1018, 1709], 'Trp21–Val/Ile22': [1021], 'bond': [1022], 'potent': [1026], 'vasoconstrictors': [1027], '(14Turner': [1029], 'A.J.': [1030, 1084, 1308], 'Murphy': [1031], 'L.J.': [1032], 'Pharmacol.': [1034], '51:': [1036], '91-102Crossref': [1037], '(223)': [1040], 'addition': [1044, 3129, 3674, 4017, 4389], 'specific': [1047, 2415, 4030], 'cleavage': [1048], 'event,': [1049], 'several': [1056], 'active': [1058, 3269, 3608, 3772, 3776], 'vitro,': [1061], 'including': [1062, 1149, 2265], 'bradykinin,': [1063], 'neurotensin,': [1064], 'substance': [1065], 'P,': [1066], 'oxidized': [1068], 'insulin': [1069], 'B': [1070, 3039, 3055, 4120, 4132, 4208, 4221], 'chain': [1071, 2638], 'cleaving': [1073], 'amino': [1076, 2579, 4291], 'side': [1077], 'hydrophobic': [1079], 'residues': [1080], '(15Hoang': [1081], 'M.V.': [1082], 'Turner': [1083, 1307], '1997;': [1087, 1311, 1459, 1531, 1593, 1667, 1735, 1877, 2374, 3398], '327:': [1088], '23-26Crossref': [1089], '(99)': [1092], '16Johnson': [1095], 'G.D.': [1096, 3748], 'Stevenson': [1097, 3749], 'T.': [1098, 1422, 1426, 1909, 1958, 2022, 2121, 2427, 2429, 2439, 2596, 3750, 4097], 'Ahn': [1099, 3751], '4053-4058Abstract': [1106, 3758], '(132)': [1114, 3766], 'Two': [1117], 'different': [1118], 'cloned.': [1123, 2460], 'first': [1125, 4289], 'identified,': [1126], 'ECE-1,': [1127, 2344, 2744], 'abundantly': [1129, 2260], 'expressed': [1130, 1143, 1773, 2261, 2301], 'vascular': [1133], 'organs': [1138], 'widely': [1142], 'nonvascular': [1145], 'tissues': [1148, 2254, 2264, 2280], 'lung,': [1150], 'pancreas,': [1151], 'testis,': [1152], 'ovary,': [1153], 'adrenal': [1155, 2270], 'gland': [1156], '(17Xu': [1157, 1347, 2922, 3000], 'Emoto': [1159, 1349, 2010, 2322, 2924, 3002, 3899], 'Giaid': [1161, 1351, 2926, 3004], 'Slaughter': [1163, 1353, 2928, 3006], 'Kaw': [1165, 1355, 2930, 3008, 3897], 'deWit': [1167, 1357, 2932, 3010], 'Yanagisawa': [1169, 1359, 1910, 1959, 2023, 2089, 2195, 2283, 2330, 2384, 2934, 3012, 3901], 'Cell.': [1171, 1361, 2936, 3014], '78:': [1173, 1363, 2938, 3016], '473-485Abstract': [1174, 1364, 2939, 3017], '(862)': [1180, 1370, 2945, 3023], '18Davenport': [1183], 'A.P.': [1184, 1263, 2238], 'Kuc': [1185, 1264], 'R.E.': [1186, 1265, 2319], 'Plumpton': [1187, 1266], 'Mockridge': [1189, 1268], 'J.W.': [1190, 1269], 'Barker': [1191, 1270], 'P.J.': [1192, 1271], 'Huskisson': [1193, 1272], 'N.S.': [1194, 1273], 'Histochem.': [1195, 1213, 1274], '30:': [1198, 1277], '359-374Crossref': [1199, 1278], '(31)': [1201, 1280], '19Korth': [1204], 'Bohle': [1206], 'R.M.': [1207], 'Corvol': [1208, 3392], 'Pinet': [1210, 3394], 'Cytochem.': [1214], '47:': [1216], '447-462Crossref': [1217], '(78)': [1220], 'comprehensive': [1224], 'analysis': [1225, 2209, 2251], 'examining': [1226], 'activity': [1229, 2067, 2455, 2908], 'expression': [1231, 1647, 2223, 2275], 'reported.': [1238, 2115], 'Studies': [1239], 'have,': [1240], 'detected': [1242, 1289, 1648, 1847, 2276, 3321, 4066], 'immunoreactivity': [1245, 1286, 1845], 'fibers': [1247], 'glial': [1250], 'limitans': [1251], 'neuronal': [1253], 'processes': [1254], 'bodies': [1257], 'cerebral': [1260, 2266], 'cortex': [1261], '(18Davenport': [1262], 'rats,': [1284], 'pyramidal': [1291], 'hippocampus': [1295], 'cultured': [1298, 2825, 2857, 2882, 3522], 'primary': [1299], 'astrocytes': [1300], '(20Barnes': [1301], 'Walkden': [1303], 'B.J.': [1304], 'Wilkinson': [1305], '68:': [1312], '570-577Crossref': [1313], '(37)': [1316, 2246], 'Four': [1319], 'isoforms': [1320, 1496], 'differing': [1324], 'cytoplasmic': [1328], 'tail': [1329, 1825, 2049], 'single': [1334], 'gene': [1335, 2084, 2111, 2163], 'located': [1336, 1767, 2166], 'chromosome': [1338, 2169], '1': [1339, 2725, 2977, 3101, 3152, 3179, 3532, 3659, 3960, 4352], '(1p36)': [1340], 'use': [1343], 'alternate': [1345], 'promoters': [1346], '21Valdenaire': [1373], 'O.': [1374, 1444, 1468, 1516, 1540, 1578, 1602, 1652, 1675, 1720, 1862, 2133, 2359], 'Rohrbacher': [1375], 'Mattei': [1377], 'M.G.': [1378], '270:': [1383, 2095, 2201, 2289, 2390], '29794-29798Abstract': [1384], '(208)': [1392], '22Shimada': [1395], 'Takahashi': [1397], 'Ikeda': [1399], 'Tanzawa': [1401], '371:': [1406], '140-144Crossref': [1407], '(116)': [1410], '23Schmidt': [1413], 'Kroger': [1415], 'Jacob': [1417], 'Seulberger': [1419], 'H.': [1420, 1430, 1905, 1954, 2018, 2129, 2317, 2423], 'Subkowski': [1421], 'Otter': [1423], 'Meyer': [1425], 'Schmalzing': [1427], 'G.': [1428, 1472, 1544, 1606, 1679, 1782, 1804, 3387], 'Hillen': [1429], '356:': [1434], '238-243Crossref': [1435], '(184)': [1438], '24Schweizer': [1441], 'Valdenaire': [1443, 1515, 1577, 1651, 1719, 1861, 2358], 'Nelbock': [1445, 1517, 1579, 1653, 1721, 1863, 2360], 'Deuschle': [1447, 1519, 1581, 1655, 1723, 1865, 2362], 'U.': [1448, 1520, 1582, 1656, 1724, 1866, 2363], 'Dumas': [1449, 1521, 1583, 1657, 1725, 1867, 2364], 'Milne': [1450, 1522, 1584, 1658, 1726, 1868, 2365], 'Edwards': [1451, 1523, 1585, 1659, 1727, 1869, 2366], 'J.B.': [1452, 1482, 1524, 1554, 1586, 1616, 1660, 1689, 1728, 1870, 2367], 'Stumpf': [1453, 1525, 1587, 1661, 1729, 1871, 2368], 'J.G.': [1454, 1526, 1588, 1662, 1730, 1872, 2369], 'Loffler': [1455, 1527, 1589, 1663, 1731, 1873, 2370], 'B.M.': [1456, 1528, 1590, 1664, 1732, 1874, 2371], '328:': [1460, 1532, 1594, 1668, 1736, 1878, 2375], '871-877Crossref': [1461, 1533, 1595, 1669, 1737, 1879, 2376], '(192)': [1464, 1536, 1598, 1672, 1740, 1882, 2379], '25Valdenaire': [1467, 1539, 1601], 'Lepailleur-Enouf': [1469, 1541, 1603, 1676], 'Egidy': [1471, 1543, 1605, 1678, 3386], 'Thouard': [1473, 1545, 1607, 1680], 'Barret': [1475, 1547, 1609, 1682], 'Vranckx': [1477, 1549, 1611, 1684], 'Tougard': [1479, 1551, 1613, 1686], 'Michel': [1481, 1553, 1615, 1688], 'Eur.': [1483, 1555, 1617, 1690], '264:': [1487, 1559, 1621, 1694, 2536], '341-349Crossref': [1488, 1560, 1622, 1695], '(148)': [1491, 1563, 1625, 1698], 'four': [1495], 'cleave': [1497], 'ETs': [1499, 2355], 'equal': [1501, 3207, 3932], 'efficiency': [1502], 'differ': [1504], 'primarily': [1505], 'subcellular': [1508], 'localization': [1509, 1983], 'tissue': [1511], 'distribution': [1512], '(24Schweizer': [1513, 1575, 1649, 1717, 1859, 2356], 'Human': [1566, 1628, 2507], 'ECE-1a': [1567, 1937, 1988, 2001, 2052, 2630, 2666, 2720, 3414], 'localized': [1569, 1638, 1708, 1894, 2185], 'predominantly': [1570, 1639, 1895], 'plasma': [1573, 1642, 1898], 'ECE-1c': [1629], 'ECE-1d': [1631], 'additional': [1645], 'Scholar,25Valdenaire': [1674], 'contrast,': [1702], 'ECE-1b': [1704, 1765, 1827, 2632, 2670, 2732], 'intracellularly.': [1710], 'Co-immunolocalization': [1711], 'studies': [1712], 'performed': [1713, 3272, 3307], 'Schweizeret': [1715], 'al.': [1716, 1760, 1798, 2012, 2119, 2999, 3362, 3383], 'Scholar)': [1742, 1816, 2042, 2143, 3379, 3405, 3768, 3914], 'ECE-1b-transfected': [1745], 'CHO': [1746, 2561, 2854, 2948, 3304, 3500, 3834, 3841, 3994], 'indicate': [1748, 2496], 'presence': [1750, 3119, 3830, 4375], 'isoform': [1753], '(TGN).': [1758], 'Azaraniet': [1759], 'similarly': [1761], 'demonstrated': [1762, 1817], 'was': [1766, 1828, 1846, 2509, 2563, 2576, 2612, 2689, 2701, 2721, 2733, 2751, 2760, 2909, 2920, 3028, 3138, 3218, 3229, 3283, 3293, 3320, 3406, 3428, 3475, 3493, 3538, 3571, 3586, 3610, 3638, 3683, 3695, 3727, 3779, 3788, 3812, 3821, 3843, 3857, 3976, 3986, 4024, 4081, 4155, 4234, 4239, 4397, 4414, 4461], 'compartment': [1771], 'when': [1772], 'Madin-Darby': [1775], 'canine': [1776], 'kidney': [1777], '(26Azarani': [1779], 'Boileau': [1781, 1803], '333:': [1788], '439-448Crossref': [1789], '(38)': [1792], 'Scholar),': [1794, 2542], 'Cailler': [1796], 'et': [1797, 2011, 2118, 2998, 3382], '(27Cailler': [1799], 'Zappulla': [1801], 'J.P.': [1802], '341:': [1810], '119-126Crossref': [1811], '(13)': [1814], 'dileucine': [1820], 'motif': [1821], 'cytosolic': [1824], 'probably': [1829, 2179], 'localization.': [1834], 'ECE-1b-': [1838], 'ECE-1c-expressing': [1840], 'line,': [1842], 'ECV304,': [1843], 'Golgi-like': [1850], 'structures': [1851], 'well': [1853, 4466], 'surface': [1858], 'Bovine': [1885, 2073], 'ECE-1b,': [1886], 'corresponds': [1888], 'ECE-1c,': [1891], '(28Emoto': [1900, 1949, 2013], 'Nurhantari': [1902, 1951, 2015], 'Alimsardjono': [1904, 1953, 2017], 'Xie': [1906, 1955, 2019], 'Yamada': [1908, 1957, 2021], 'Matsuo': [1912, 1961, 2025], '1509-1518Abstract': [1919, 1968, 2032], '(44)': [1927, 1976, 2040], 'However,': [1930, 2116], 'contrast': [1932], 'ECE-1a,': [1935], 'bovine': [1936, 1987, 2051, 2161, 2175, 2253, 2409, 2835, 2889, 3656, 4349], 'convincingly': [1939], 'shown': [1941], 'constitutively': [1944], 'targeted': [1945], 'lysosome': [1948], 'This': [1979], 'difference': [1980], 'between': [1981, 2005], 'fact': [1994], 'isoform-specific': [1997], 'N-terminal': [1998, 2048], 'region': [1999], 'poorly': [2003], 'conserved': [2004, 2056], 'species.': [2007], 'fact,': [2009], 'identified': [2043], 'lysosome-targeting': [2044], 'signals': [2045], 'ECE-1a.': [2059], 'ECE-2': [2060, 2074, 2110, 2162, 2183, 2212, 2225, 2257, 2299, 2345], 'homologous': [2063], 'catalytic': [2066], 'similar': [2068, 3356], 'ECE-1.': [2072], 'cloned': [2077, 2634], 'encoded': [2080], 'separate': [2083], '(29Emoto': [2087, 2193, 2281], '15262-15268Crossref': [2096, 2202, 2290, 2391], '(434)': [2099, 2205, 2293, 2394], 'sequence': [2103, 3442, 3471], 'chromosomal': [2105], 'location': [2106], 'Nagase': [2117], '(30Nagase': [2120], 'Ishikawa': [2122], 'Miyajima': [2124], 'Tanaka': [2126], 'Kotani': [2128], 'Nomura': [2130], 'Ohara': [2132], 'DNA': [2134], '5:': [2137], '31-39Crossref': [2138], '(179)': [2141], 'recently': [2144, 2405], 'cloning': [2147], 'unidentified': [2150], 'cDNA,': [2153], 'KIAA0604,': [2154], 'shares': [2156], '93%': [2157], 'identity': [2158], '3.': [2170, 2727, 2739], 'Given': [2171], 'similarity': [2173], 'ECE-2,': [2176], 'cDNA': [2178], 'represents': [2180], 'ECE-2.': [2182], 'acidic': [2190, 2227], 'pH': [2191, 3043, 3553, 3649, 3745, 3955, 4345], 'optimum': [2192], 'Immunocytochemical': [2208], 'HUVECs': [2214, 2823], 'revealed': [2215, 2255], 'punctate': [2217], 'pattern': [2218], 'staining': [2220, 3601, 3811], 'consistent': [2221], 'vesicles': [2229], 'constitutive': [2232], '(31Russell': [2235], 'F.D.': [2236], 'Davenport': [2237], 'Circ.': [2239], '84:': [2242], '891-896Crossref': [2243], 'Northern': [2249], 'blot': [2250], 'neural': [2263], 'cortex,': [2267], 'cerebellum,': [2268], 'medulla,': [2271], 'low': [2273], 'level': [2274], 'many': [2278], 'other': [2279], 'mouse': [2297], 'brain,': [2298], 'heterogeneous': [2303], 'populations': [2304], 'neurons': [2306], 'thalamus,': [2309], 'hypothalamus,': [2310], 'amygdala,': [2311], 'dentate': [2312], 'gyrus,': [2313], 'CA3': [2315], '(32Yanagisawa': [2316], 'Hammer': [2318], 'Richardson': [2320], 'Williams': [2324], 'S.C.': [2325], 'Takeda': [2326], 'Clouthier': [2328], 'D.E.': [2329], 'Clin.': [2333], 'Invest.': [2334], '105:': [2336], '1373-1382Crossref': [2337], '(167)': [2340], 'Like': [2343], 'cleaves': [2346], 'ET-1': [2348, 3084, 3621, 3635, 3681, 3737, 4448, 4477], 'efficiently': [2350], 'among': [2351], 'three': [2353], '29Emoto': [2382], 'Another': [2397], 'member': [2398], 'family,': [2402], 'ECE-3,': [2403], 'purified': [2407, 2915, 3844], 'iris': [2410], 'microsomes': [2411], 'highly': [2414, 2914, 4029], 'conversion': [2418, 3262], 'ET-3': [2421], '(33Hasegawa': [2422], 'Hiki': [2424], 'Sawamura': [2426], 'Aoyama': [2428], 'Okamoto': [2430], 'Miwa': [2432], 'Shimohama': [2434], 'Kimura': [2436], 'Masaki': [2438], '428:': [2443], '304-308Crossref': [2444], '(63)': [2447], 'yet': [2458], 'previously': [2473, 2515], 'explored.': [2474], 'Here': [2475], 'present': [2477], 'pharmacological': [2479], 'biochemical': [2481], 'evidence': [2482], 'can': [2485], 'vivo.': [2493], 'These': [2494], 'data': [2495], 'potential': [2498], 'measured': [2510, 2564, 3684], 'sandwich': [2512, 3141, 3236, 3686, 4031, 4400], 'ELISA': [2513, 3142, 3237, 3687, 4401], 'described': [2516, 2995, 3239, 3359], '(34Suzuki': [2517], 'Cheung': [2519], 'T.T.': [2520], 'Cai': [2521], 'X.D.': [2522], 'Odaka': [2523], 'Otvos': [2525], 'Jr.,': [2526], 'Golde': [2530], 'Science.': [2534], '1336-1340Crossref': [2537], '(1354)': [2540], 'using': [2543, 2565, 2658, 2694, 2704, 2723, 2735, 2753, 2782, 3308, 3415, 3430, 3565, 4027, 4114, 4187, 4285, 4298, 4402], 'BAN50/BA27': [2545, 4404], 'BAN50/BC05': [2547], 'antibody': [2548, 2570, 2574, 3310, 3319, 3330, 4039], 'systems': [2549], '(Takeda)': [2550, 2575], 'detect': [2552, 3315], 'Aβ42,': [2555], 'respectively.': [2556], 'Hamster': [2557], 'derived': [2559], 'BNT77/BA27': [2567], 'BNT77/BC05': [2569], 'systems.': [2571], 'BNT77': [2573], 'raised': [2577], 'against': [2578, 3546], 'acids': [2580, 4292], '11–28': [2581], 'recognize': [2585], 'amino-terminally': [2586], 'modified': [2587], 'truncated': [2589], '(35Asami-Odaka': [2591], 'Ishibashi': [2593], 'Kikuchi': [2595], 'Kitada': [2597], 'Suzuki': [2599], 'Biochemistry.': [2601], '34:': [2603], '10272-10278Crossref': [2604], '(150)': [2607], 'comparing': [2615], 'values': [2616], 'obtained': [2617, 2622], 'samples': [2619, 4172], 'those': [2621], 'standards': [2628], '(Bachem).': [2629], 'were': [2633, 2770, 2792, 2810, 2819, 2824, 2856, 2881, 2950, 2967, 2992, 3072, 3114, 3125, 3147, 3158, 3164, 3200, 3271, 3306, 3512, 3670, 3851, 4013, 4104, 4141, 4173, 4201, 4246, 4250, 4264, 4296, 4364, 4385, 4480], 'reverse': [2636, 2702, 2707], 'transcription-polymerase': [2637], 'reaction': [2639, 3975, 3998], 'two': [2641, 4290], 'fragments': [2642, 2769, 4103], '(joined': [2643], 'unique': [2646], 'PvuII': [2647, 2675, 2680, 2775], 'site)': [2648], '(HUVEC,': [2655], 'ATCC)': [2656], 'RNA': [2657, 2688], 'following': [2660, 3417], 'primers': [2661, 2724, 2736, 2754], '(restriction': [2662], 'sites': [2663, 3434], 'underlined):': [2664], '1)': [2665], 'forward,': [2667, 2671, 2681, 3420], '5′-CAGGAATTCGCCACCATGCCTCTCCAGGGCCTGGGCCTGC-3′;': [2668], '2)': [2669], '5′-CAGGAATTCGCCACCATGCGGGGCGTGTGGCCGCCC-3′;': [2672], '3)': [2673], 'reverse,': [2676, 2686, 3423], '5′-CAGCAGCTTCCCCAGCTGGACC-3′;': [2677], '4)': [2678], '5′-GGTCCAGCTGGGGAAGCTGCTG-3′;': [2682], '5)': [2684], '5′-GCTCTAGATTACCAGACTTCGCACTTGTGAGGCGGG-3′.': [2687], 'prepared': [2690, 2993], 'HUVEC': [2692], 'Qiagen': [2696], 'RNeasy': [2697], 'miniprep': [2698], 'kit': [2699], 'transcribed': [2703], 'Superscript': [2705], 'transcriptase': [2708], 'oligo(dT)': [2711], 'primer': [2712], '(Roche': [2713, 2955, 3186, 3311], 'Molecular': [2714, 2800, 2956, 3187, 3312, 3483], 'Biochemicals).': [2715], '5′': [2717, 2729, 2766], 'fragment': [2718, 2730, 2742], 'amplified': [2722, 2734, 2752], '2': [2737, 4089], '3′': [2741, 2768], 'isoforms,': [2750], '4': [2755], '5.Pfu': [2757], 'polymerase': [2758], '(Stratagene)': [2759], 'used': [2761, 2921, 3852, 3972], 'amplifications.': [2764], 'ligated': [2771], 'together': [2772], 'subcloned': [2778, 3429], 'pcDNA3': [2780], '(Invitrogen)': [2781], 'primer-encodedEcoRI': [2783, 3431], 'XbaI': [2785], 'sequences': [2788], 'constructs': [2791], 'confirmed': [2793, 3476], 'dideoxy': [2795, 3478], 'sequencing': [2796, 3479], 'Mayo': [2799, 3482, 3923], 'Biology': [2801, 3484], 'Core': [2802, 3485, 3919], 'Facility.': [2803, 3486], 'Unless': [2804], 'otherwise': [2805], 'noted,': [2806], 'culture': [2808, 3216], 'reagents': [2809, 3339], 'purchased': [2811, 2820], 'Life': [2813], 'Technologies,': [2814], 'Inc.,': [2815], 'ATCC.': [2822], "Kaighn's": [2827], 'F12K': [2828], 'medium': [2829, 2861, 3183, 3204, 3217, 3282, 3528, 3537, 3838], '(ATCC)': [2830], 'supplemented': [2831, 2863, 2885], '10%': [2833, 2865, 2887], 'fetal': [2834, 2888], 'serum,': [2836, 2868, 2890], '0.1': [2837, 3081, 3090], 'mg/ml': [2838, 2841, 2978, 2986], 'heparin,': [2839], '0.03': [2840], 'growth': [2844, 3182, 3203], 'supplement': [2845], '(Sigma),': [2846, 3190], '100': [2847, 2851, 2869, 2873, 2891, 2895, 3105, 3581, 3652, 3662, 3962, 4355], 'units/ml': [2848, 2870, 2892], 'penicillin,': [2849, 2871, 2893], 'μg/ml': [2852, 2874, 2896], 'streptomycin.': [2853, 2875, 2897], "Ham's": [2859], 'F-12': [2860], '(BioWhittaker)': [2862], 'newborn': [2866], 'calf': [2867], 'H4': [2876], '(human': [2878], 'neuroglioma': [2879], 'origin)': [2880], 'Opti-MEM': [2884], 'For': [2898, 3061], 'passaging': [2899], 'prior': [2902, 3154, 3557, 3941], 'experiments': [2904], 'measured,': [2912], 'trypsin': [2916], '(Sigma': [2917], 'T-7418)': [2918], 'solution': [2919, 3171, 3519], 'transfected': [2951], 'FuGENE': [2953], '6': [2954, 4335], 'Biochemicals)': [2957, 3313], 'according': [2958, 3871], 'directions': [2961], 'manufacturer.': [2964], 'Stable': [2965], 'generated': [2968, 3407, 3494], 'selecting': [2970, 3503], 'pcDNA3-transfected': [2971], '(ECE-1a': [2973], 'ECE-1b)': [2975], 'Geneticin': [2979], 'pSecTag-transfected': [2981], '(solECE-1)': [2983], '0.8': [2985], 'Zeocin': [2987, 3505], '(Invitrogen).': [2988], 'Cell': [2989], 'fractions': [2991, 3067, 4245], 'Xu': [2997], 'Protein': [3026], 'BCA': [3031], '(Pierce)': [3033], 'membranes': [3035, 3051], 'resuspended': [3036], 'buffer': [3038, 3054, 3094, 3579, 3999, 4125, 4133, 4216, 4222], '(20': [3040], 'mm': [3041, 3047, 3533, 3582, 3647, 3653, 3660, 3953, 4020, 4342, 4353], 'Tris-HCl,': [3042], '7.4,': [3044], 'containing': [3045, 3056, 3097, 3184, 3205, 3531, 3580, 3651, 3957, 4347], '250': [3046], 'sucrose)': [3048], 'solubilized': [3052], '2.5%': [3057], 'C12E10(polyoxyethylene-10-lauryl': [3058], 'ether,': [3059], 'Sigma).': [3060], 'measurement': [3062], '(10–50': [3068], 'μg': [3069], 'protein)': [3071], 'incubated': [3073, 3174, 3201, 3987, 4082, 4156, 4319, 4481], '30': [3075], 'min': [3076, 3861, 4123, 4214], '37': [3078, 3641, 4004, 4168, 4338], '°C': [3079, 3642, 4005, 4339], 'μm': [3082, 3106, 3110, 3666, 3963, 4356, 4360], '(1–38)': [3085], '(American': [3086], 'Peptide': [3087], 'Co.)': [3088], 'm': [3091, 3099, 3550, 3631, 3721, 3734, 4312, 4426, 4438, 4469], 'sodium': [3092, 3534, 3551], 'phosphate': [3093], '(pH': [3095], '6.8)': [3096], '0.5': [3098], 'NaCl,': [3100, 3556, 3654], 'mmphenylmethylsulfonyl': [3102], '(PMSF),': [3104], 'leupeptin,': [3107, 3964, 4357], '20': [3109, 3665, 3966, 4330, 4359], 'pepstatin.': [3111, 3667, 4361], 'Duplicate': [3112], 'reactions': [3113, 3669, 4012, 4363, 4384], 'carried': [3115, 4365], 'out': [3116, 4366], '(100': [3122, 4162, 4381], 'μm).': [3123, 4382], 'Reactions': [3124], 'stopped': [3126, 3671, 4014, 4386], 'EDTA': [3131, 3676, 4391], '(5': [3132, 3677, 4392], 'mm),': [3133, 3678, 4393], 'mature': [3135, 3680], 'ET-1-(1–21)': [3136], 'quantitated': [3139], '(Amersham': [3143, 3331, 3340, 3688], 'Pharmacia': [3144, 3332, 3341, 3689], 'Biotech).': [3145, 3690], 'Cells': [3146], 'passaged': [3148], 'six-well': [3150], 'plates': [3151], 'day': [3153], 'utilized': [3159], 'confluence.': [3161], 'Triplicate': [3162], 'wells': [3163], 'washed': [3165, 3513], 'twice': [3166, 3514], "Hanks'": [3168, 3516], 'balanced': [3169, 3517], '17–24': [3176], 'h': [3177, 3525, 4166, 4336], 'ml': [3180], 'Biochemicals),': [3188], 'thiorphan': [3189], 'captopril': [3192], '(Sigma)': [3193], 'indicated': [3196, 3278, 4008], 'concentrations.': [3197], 'Control': [3198, 4362], 'vehicle': [3210, 3935], '(phosphate-buffered': [3211], 'saline).': [3212], 'After': [3213], 'treatment,': [3214], 'harvested': [3219], 'spun': [3221], '14,000': [3223], '×': [3224, 3722, 3742, 4090, 4196], 'g,': [3225], 'supernatant': [3228], 'analyzed': [3230, 4026, 4251], 'APP': [3243], 'Western': [3245, 3301, 3337], 'blot.': [3246], 'To': [3247, 3826, 4070], 'assess': [3248], 'cellular': [3249], 'toxicity': [3250], 'compounds,': [3253], 'MTS': [3254, 3264], 'assays': [3255, 4413], '(CellTiter': [3256], '96®,': [3257], 'Promega),': [3258], 'measure': [3260], 'formazan': [3266], 'metabolically': [3268], 'after': [3276], 'time': [3279, 4009], 'points.': [3280], 'Culture': [3281], 'subjected': [3284], 'electrophoresis': [3286, 3598], '10–20%': [3288], 'Tricine': [3289], 'gels': [3290], '(Novex)': [3291], 'subsequently': [3294], 'transferred': [3295], 'Immobilon': [3297], 'P': [3298], '(Millipore': [3299], 'Corp.).': [3300], 'blots': [3302], '22C11': [3309], 'APP.': [3317], 'Bound': [3318, 3569], 'incubation': [3323, 3943, 4002], 'appropriate': [3326], 'horseradish': [3327], 'peroxidase-linked': [3328], 'secondary': [3329], 'Biotech)': [3333, 3342], 'ECL': [3336], 'blotting': [3338], 'exposure': [3344], 'x-ray': [3346], 'film.': [3347], 'construct': [3349, 3427, 3474, 3498], 'encoding': [3350], 'Ahnet': [3361], '(36Ahn': [3363], 'Herman': [3365, 3879], 'S.B.': [3366, 3880], 'Fahnoe': [3367], 'D.C.': [3368], 'Arch.': [3369], '359:': [3373], '258-268Crossref': [3374], '(36)': [3377], 'Korth': [3381], '(37Korth': [3384], 'Parnot': [3388], 'LeMoullec': [3390], 'J.M.': [3391], '417:': [3399], '365-370Crossref': [3400], '(34)': [3403], 'amplifying': [3409], 'domain': [3412], 'primers:': [3418], 'SolECE-1': [3419, 3422, 3856], '5′-GAGAGAATTCTCAGTACCAGACAAGATCCCC-3′;': [3421], '5′-CGTTTTCCTTTTGCGGCCGCCCAGACTTCGCACTTGTGAGG-3′.': [3424], 'solECE-1': [3426, 3489, 3585, 3609, 3644, 3740, 3820, 4152, 4377], 'NotI': [3433], 'pSecTag2B': [3436], '(Invitrogen),': [3437], 'incorporates': [3439], 'leader': [3441], 'onto': [3443], 'N': [3445, 4043], 'terminus': [3446, 3464, 4044], 'secretion': [3451], 'mammalian': [3453], 'sequential': [3456], 'c-Myc': [3457], 'His6': [3459, 3563], 'tags': [3460], 'C': [3463], 'facilitate': [3466], 'detection': [3467], 'purification.': [3469], 'stable': [3488], 'secreting': [3490], 'line': [3492], 'transfecting': [3496], '(0.8': [3506], 'mg/ml).': [3507], 'At': [3508], 'confluence,': [3509], '48': [3524], 'serum-free': [3527, 3837], '(CHO-S-SFM': [3529], 'II)': [3530], 'butyrate.': [3535], 'Conditioned': [3536], 'filtered': [3539], '0.2-μm': [3542], 'filter': [3543], 'dialyzed': [3545], 'binding': [3547, 3578, 4037], 'buffer,': [3548, 4344], '0.05': [3549], 'phosphate,': [3552], '8.0,': [3554], '0.3m': [3555], 'purification': [3559], 'via': [3560, 4036], 'C-terminal': [3562], 'tag': [3564], 'Ni2+-nitrilotriacetic': [3566, 3575, 3989], 'acid-agarose': [3567, 3576], '(Qiagen).': [3568], 'eluted': [3572, 3849, 4202], 'imidazole.': [3583], 'initially': [3587], 'estimated': [3588, 3612, 3780, 3794, 3977], '≤25%': [3591], 'pure,': [3592], 'assessed': [3594], 'SDS-polyacrylamide': [3596], 'gel': [3597], 'Coomassie': [3600, 3810], '(data': [3602], 'shown).': [3604], 'determining': [3614], 'second': [3616, 3692, 4433, 4453], 'order': [3617, 3693, 4434, 4454], 'rate': [3618, 3626, 3694, 3700, 4233, 4407, 4435, 4455], 'hydrolysis': [3622, 3738, 4315, 4410, 4442, 4449, 4457], 'dividing': [3624], 'publishedk': [3629], 'cat/K': [3630, 3720, 3733, 4437], '.': [3632, 4470], 'Specifically,': [3633, 4471], '(0.1': [3636, 4478], 'μm)': [3637, 3929, 3950, 4163, 4331, 4474, 4479], 'reacted': [3639], '50': [3646, 3952, 4341], 'MES,': [3648, 3954], '6.5,': [3650, 3956, 4346], '0.05%': [3655, 4348], 'serum': [3657, 4350], 'albumin,': [3658, 4351], 'PMSF,': [3661, 4354], 'μmleupeptin,': [3663], 'apparent': [3699], 'constantk': [3701], 'Equation': [3703], '1,': [3704], 'y=1−e−ktwhere': [3705], 'y': [3706], 'fraction': [3709], 'substrate': [3711, 4419, 4460, 4464], 'hydrolyzed': [3712], 'andt': [3713], 'time.': [3716], 'Since': [3717], 'k': [3718, 3726], '=k': [3719], '[E],': [3723], 'calculated': [3725], 'divided': [3729], 'reportedk': [3732], '(2.52': [3741], '104m−1': [3743], 's−1at': [3744], '6.5)': [3746], '(16Johnson': [3747], 'determine': [3770, 4309], 'concentration.': [3774], 'method': [3783], 'throughout': [3784], 'report': [3786], '∼30-fold': [3789], 'lower': [3790], 'than': [3791], 'originally': [3793], 'Coomassie-stained': [3800], 'gel,': [3801], 'suggesting': [3802], 'either': [3803], 'our': [3805], 'estimation': [3806], 'purity': [3808], 'incorrect': [3813], 'majority': [3817], 'form.': [3825], 'control': [3827, 3854], 'co-purifying': [3832], 'native': [3833], 'proteins,': [3835], 'conditioned': [3836], 'nontransfected': [3840, 3993], 'above,': [3846], 'proteins': [3850, 3991], 'experiments.': [3855], 'preincubated': [3858], '15': [3860], 'room': [3863], 'temperature': [3864], 'PD069185': [3869], '(synthesized': [3870], 'published': [3873], 'methods': [3874], '(38Ahn': [3875], 'Sisneros': [3877], 'A.M.': [3878, 3892], 'Pan': [3881], 'S.M.': [3882], 'Hupe': [3883], 'Nikam': [3887], 'Cheng': [3889], 'X.M.': [3890], 'Doherty': [3891], 'Schroeder': [3893], 'R.L.': [3894], 'Haleen': [3895], 'S.J.': [3896], 'Commun.': [3906], '243:': [3908], '184-190Crossref': [3909], '(51)': [3912], 'Organic': [3917], 'Chemistry': [3918], 'Facility': [3920], 'Clinic': [3924], 'Jacksonville)': [3925], '(150': [3928], 'volume': [3933], '(Me2SO': [3936], 'phosphate-buffered': [3938], 'saline,': [3939], 'respectively),': [3940], '(0.01': [3949], '0.01%': [3958], 'C12E10,': [3959], 'mmPMSF,': [3961], 'μmpepstatin.': [3967], 'solECE': [3971], '∼6': [3980], 'nm.': [3981, 4243], 'control,': [3984], 'acid-purified': [3990], 'alone.': [4000], 'Following': [4001], 'points,': [4010], '5': [4019], 'EDTA.': [4021], 'ELISA,': [4032], 'captures': [4034], 'BAN50': [4040], 'detects': [4046], 'full-length': [4047], 'ending': [4049], 'position': [4051], '40': [4052], '(BA27)': [4053], '42': [4055], '(BC05).': [4056], 'Thus,': [4057], 'cleaved': [4062], 'will': [4063], 'assay.': [4069], 'analyze': [4072], 'solECE-1,': [4078, 4317], 'above': [4084, 4158], 'with3H-radiomethylated': [4085], '(0.56': [4087], 'μm,': [4088], '104': [4091], 'dpm),': [4092], 'gift': [4094], 'Dr.': [4096], 'Rosenberry.': [4099], 'resolved': [4105], 'reversed-phase': [4107, 4190], 'HPLC': [4108], 'Vydac': [4111], 'C4': [4112, 4189], 'column': [4113, 4191], 'linear': [4116, 4415], 'gradient': [4117, 4205], '0–100%': [4119], '60': [4122], '(A': [4124, 4215], '=': [4126, 4134, 4217, 4223], '0.1%': [4127, 4135, 4218], 'trifluoroacetic': [4128, 4136, 4219, 4229], 'water;': [4131], 'acetonitrile).': [4139], 'Fractions': [4140], 'collected': [4142, 4262], 'counted': [4144], 'Beckman': [4147], 'LS': [4148], '6500': [4149], 'scintillation': [4150], 'counter.': [4151], '(∼20': [4153], 'nm)': [4154, 4323], '17': [4165], '°C.': [4169], 'digest': [4171], 'run': [4174], 'Applied': [4177, 4300], 'Biosystems': [4178, 4269, 4301], '130A': [4179], 'Separation': [4180], 'System': [4181], '(Applied': [4182], 'Biosystems,': [4183], 'Foster': [4184], 'City,': [4185], 'CA)': [4186], '(Brownlee': [4192], 'Aquapore': [4193], 'BU-300;': [4194], '2.1': [4195], '220': [4197], 'mm).': [4198], '5–80%': [4207], 'over': [4209], 'period': [4211], '69': [4213], 'acid;': [4220], '80%': [4224], 'acetonitrile,': [4225], '20%': [4226], 'water,': [4227], '0.09%': [4228], 'acid).': [4230], 'flow': [4232], '200': [4235], 'μl/min,': [4236], 'absorbance': [4238], 'monitored': [4240], '215': [4242], 'Half-minute': [4244], 'collected,': [4247], 'peaks': [4249], 'mass': [4253], 'spectrometry': [4254], 'Edman': [4256], 'sequencing.': [4257], 'masses': [4259], 'PerSeptive': [4268], 'MALDI-TOF': [4270], 'Voyager': [4271], 'DE-RP': [4272], 'Mass': [4273], 'Spectrometer': [4274], '(Framingham,': [4275], 'MA)': [4276], 'operated': [4277], 'delayed': [4280], 'extraction': [4281], 'reflector': [4283], 'mode': [4284], 'α-cyano-4-hydroxycinnamic': [4286], 'acid.': [4287], 'Procise': [4302], '492': [4303], 'sequencer.': [4304], 'attempt': [4307], 'K': [4311, 4425, 4468], '(∼17': [4322], '(3,': [4327], '10,': [4328], '0': [4333], 'MES': [4343], 'absence': [4369], 'inhibited': [4378], 'system.': [4405], 'respect': [4417], 'concentration,': [4420], 'precluding': [4421], 'determination': [4423], 'andV': [4427], 'max.': [4428], 'next': [4430], 'constant,k': [4436], ',': [4439], 'relative': [4443], 'solECE-1.': [4451], 'concentrations': [4465], 'below': [4467], '(2.5': [4473], 'triplicate': [4483], 'al': [4484]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2019042091', 'counts_by_year': [{'year': 2024, 'cited_by_count': 9}, {'year': 2023, 'cited_by_count': 8}, {'year': 2022, 'cited_by_count': 9}, {'year': 2021, 'cited_by_count': 10}, {'year': 2020, 'cited_by_count': 11}, {'year': 2019, 'cited_by_count': 8}, {'year': 2018, 'cited_by_count': 10}, {'year': 2017, 'cited_by_count': 13}, {'year': 2016, 'cited_by_count': 16}, {'year': 2015, 'cited_by_count': 13}, {'year': 2014, 'cited_by_count': 16}, {'year': 2013, 'cited_by_count': 15}, {'year': 2012, 'cited_by_count': 13}], 'updated_date': '2025-01-09T09:49:28.195358', 'created_date': '2016-06-24'}