Title: Cell density and exogenous CNTF affect CNTF mRNA levels in glial cell cultures
Abstract: REGULATION of CNTF mRNA was investigated in primary cortical astrocytes cultured from newborn rats and in C6 glioma cells. Northern blot analysis indicated that semi-confluent astrocyte cell cultures contained 8% of the CNTF mRNA of confluent cultures. CNTF added to confluent astrocyte cultures down-regulated CNTF message in a dose-dependent fashion. In contrast, when CNTF was given to semi-confluent astrocyte cultures the level of CNTF mRNA was up-regulated. C6 glioma cells also showed a cell density-dependent expression of CNTF: cells from confluent cultures expressed detectable amounts of CNTF mRNA whereas those from semi-confluent cultures did not. Thus, CNTF mRNA expression by astrogtial and glioma cells is regulated by cell contact and exogenous CNTF in vitro.
Publication Year: 1994
Publication Date: 1994-10-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 12
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