Title: Production and characterization of a C15-surfactin-O-methyl ester by a lipopeptide producing strain Bacillus subtilis HSO121
Abstract: A surfactin mono-methyl ester was isolated from the cell broth of Bacillus subtilis HSO121 by acid precipitation, methanol extraction and two-step chromatographic methods. The structure was analyzed by GC/MS, HPLC, ESI Q-TOF MS/MS and NMR. It indicates that this ester is a C15-surfactin-O-methyl ester of which the fatty acid portions are composed of iso-C15 and anteiso-C15 β-hydroxy fatty acids. Based on the critical micelle concentration (CMC) in phosphate buffered saline (PBS, pH 8.0) and the IC50 value on Hela cell lines, the C15-surfactin-O-methyl ester has a stronger surface activity and lower antitumoral effect than C15-surfactin does. It is found that the Glu residues of surfactin-like lipopeptides play a role in their anti-proliferative effects on Hela cells. The possible antitumoral activity of surfactin is discussed in relation to their structures.
Publication Year: 2009
Publication Date: 2009-06-28
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 61
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