Publication Information

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'https://openalex.org/W2050795937', 'https://openalex.org/W2017222444', 'https://openalex.org/W2017141212', 'https://openalex.org/W1986597572', 'https://openalex.org/W1978828438'], 'abstract_inverted_index': {'The': [0, 53, 112, 143, 240, 293, 352, 383, 480, 631, 739, 782, 947, 1260, 1364, 1696, 1768, 2028, 2190, 2221, 2335, 2384, 2460, 2498, 2507, 2522, 2535, 2569, 2642, 2719, 2757, 2770, 2816, 2878, 2901, 2917, 2994, 3005, 3018, 3111, 3133, 3155, 3244, 3265, 3310, 3335, 3591, 3634, 3683, 3739, 3775, 3810, 3840, 3877, 4163, 4204, 4363, 4398, 4443, 4499, 4546, 4574], 'Klenow': [1, 81, 241, 321, 515, 524, 564, 573], 'fragment': [2, 242, 516, 525, 565, 574], 'of': [3, 29, 49, 56, 60, 75, 115, 120, 141, 167, 233, 243, 269, 289, 296, 300, 315, 355, 360, 381, 407, 473, 483, 517, 527, 566, 576, 596, 633, 650, 660, 694, 706, 719, 726, 742, 752, 775, 785, 814, 824, 831, 861, 898, 928, 950, 975, 983, 996, 1006, 1017, 1055, 1063, 1173, 1183, 1219, 1262, 1275, 1278, 1289, 1302, 1330, 1336, 1341, 1353, 1367, 1373, 1384, 1426, 1473, 1475, 1495, 1499, 1521, 1586, 1591, 1602, 1623, 1633, 1683, 1698, 1705, 1713, 1720, 1732, 1738, 1751, 1793, 1800, 1812, 1817, 1827, 1842, 1864, 1900, 1909, 1915, 1923, 1934, 1941, 2030, 2038, 2067, 2094, 2108, 2127, 2245, 2249, 2329, 2420, 2428, 2455, 2509, 2517, 2540, 2577, 2628, 2709, 2726, 2737, 2761, 2772, 2774, 2809, 2828, 2845, 2856, 2881, 2903, 2909, 2924, 2933, 2964, 3002, 3032, 3100, 3106, 3140, 3178, 3184, 3206, 3262, 3293, 3312, 3355, 3362, 3368, 3439, 3463, 3486, 3492, 3523, 3551, 3569, 3593, 3599, 3637, 3685, 3693, 3703, 3710, 3719, 3765, 3784, 3788, 3802, 3813, 3831, 3854, 3884, 3905, 3916, 3943, 3956, 3963, 3969, 3976, 3982, 3995, 4005, 4018, 4028, 4037, 4053, 4060, 4066, 4077, 4087, 4097, 4107, 4116, 4144, 4165, 4208, 4225, 4239, 4246, 4252, 4261, 4264, 4281, 4285, 4295, 4297, 4343, 4400, 4467, 4477, 4481, 4501, 4523, 4533, 4583, 4597], 'Escherichia': [4, 244], 'coli': [5, 245, 487, 500, 519, 549, 568, 3625], 'DNA': [6, 61, 101, 246, 301, 341, 484, 488, 501, 520, 530, 550, 569, 579, 620, 644, 674, 753, 757, 780, 786, 801, 832, 871, 930, 1044, 1368, 1459, 1594, 1618, 1783, 1829, 1867, 2032, 2040, 2050, 2219, 2336, 2947, 2956, 3369, 3425, 3441, 3488], 'polymerase': [7, 14, 40, 65, 127, 202, 247, 254, 280, 305, 367, 442, 489, 502, 511, 521, 531, 551, 560, 570, 580, 652, 680, 700, 754, 762, 777, 790, 862, 1024, 1229, 1390, 1715, 1745, 1868, 1871, 1972, 1985, 2041, 2181, 2327, 2337, 2388, 3188, 3316, 3442, 3717, 3752, 3829, 3873, 3903, 3924, 3951, 3966, 4390], 'I': [8, 248, 490, 1343, 1450, 1869, 2097, 3348, 3571, 4287], 'houses': [9, 249], 'catalytic': [10, 250], 'centers': [11, 251], 'for': [12, 47, 215, 219, 252, 287, 455, 459, 653, 735, 1598, 1730, 1982, 2074, 2224, 2387, 2519, 2549, 2613, 2920, 2944, 2953, 3075, 3130, 3152, 3260, 3500, 3879, 3922, 4273, 4425], 'both': [13, 253, 678, 800, 1294, 1983, 3261], 'and': [15, 66, 145, 218, 255, 306, 385, 458, 604, 625, 646, 681, 723, 802, 868, 910, 954, 966, 1031, 1042, 1061, 1070, 1190, 1231, 1299, 1333, 1371, 1464, 1665, 1716, 1759, 1772, 1802, 1986, 2046, 2064, 2106, 2112, 2137, 2182, 2212, 2243, 2248, 2256, 2332, 2372, 2377, 2434, 2447, 2474, 2485, 2585, 2595, 2615, 2712, 2732, 2759, 2836, 2841, 2847, 2871, 2894, 2906, 2927, 2946, 2955, 3045, 3103, 3168, 3187, 3202, 3246, 3302, 3315, 3329, 3461, 3563, 3575, 3644, 3674, 3722, 3729, 3736, 3794, 3819, 3837, 3845, 3861, 3870, 4041, 4120, 4147, 4159, 4178, 4182, 4190, 4214, 4441, 4459, 4469, 4484, 4506, 4550, 4562], '3′–5′': [16, 131, 256, 371, 613, 1071, 1296, 2148, 2609], 'exonuclease': [17, 44, 68, 132, 205, 238, 257, 284, 308, 372, 445, 478, 506, 555, 614, 665, 683, 765, 1234, 1297, 1718, 1749, 1807, 1987, 2149, 2610, 2643, 2853, 2995, 3186, 3314, 3482, 3708, 3993, 4075, 4142, 4188, 4195, 4257, 4262, 4341, 4361, 4369, 4396, 4402, 4453, 4528], 'activities': [18, 258, 684, 1719, 3189, 3317, 3955], 'that': [19, 190, 259, 430, 676, 795, 810, 991, 1168, 1287, 1448, 1494, 1510, 1552, 1650, 1726, 1893, 2086, 3462, 3522, 3622, 3692, 3853, 3913, 4382, 4423, 4513], 'are': [20, 95, 194, 260, 335, 434, 745, 1347, 1980, 2089, 2930, 3022, 3435, 3919, 4044, 4123, 4517], 'separated': [21, 261], 'by': [22, 172, 262, 412, 485, 593, 673, 989, 1004, 1058, 1224, 1703, 1709, 1822, 2124, 2168, 2218, 2261, 2392, 2425, 2452, 2491, 2503, 2556, 2723, 2734, 2780, 2826, 3043, 3137, 3147, 3171, 3217, 3290, 3655, 3780, 3816, 3948, 3979, 4002, 4025, 4063, 4084, 4104, 4418, 4437, 4456, 4504, 4536, 4590], 'about': [23, 263, 1513, 4223, 4584, 4591], '35': [24, 264], 'Å.': [25, 265], 'Upon': [26, 266], 'the': [27, 33, 39, 50, 57, 64, 67, 76, 87, 98, 109, 126, 130, 139, 163, 182, 191, 201, 204, 208, 234, 237, 267, 273, 279, 290, 297, 304, 307, 316, 327, 338, 349, 366, 370, 379, 403, 422, 431, 441, 444, 448, 474, 477, 648, 651, 654, 661, 664, 679, 682, 688, 695, 699, 707, 711, 714, 720, 727, 732, 750, 761, 764, 771, 776, 789, 803, 811, 821, 825, 828, 834, 859, 866, 869, 896, 926, 960, 984, 994, 997, 1000, 1007, 1015, 1023, 1028, 1037, 1053, 1059, 1064, 1068, 1169, 1174, 1181, 1184, 1188, 1220, 1279, 1303, 1308, 1311, 1328, 1334, 1351, 1354, 1374, 1378, 1382, 1389, 1424, 1427, 1431, 1436, 1471, 1491, 1496, 1500, 1519, 1526, 1529, 1553, 1556, 1592, 1599, 1603, 1607, 1615, 1621, 1624, 1634, 1638, 1646, 1654, 1678, 1693, 1710, 1714, 1717, 1727, 1733, 1744, 1748, 1778, 1791, 1794, 1798, 1806, 1810, 1815, 1824, 1828, 1843, 1847, 1852, 1861, 1880, 1895, 1904, 1907, 1910, 1921, 1924, 1932, 1935, 1939, 1942, 1970, 1984, 2034, 2049, 2052, 2060, 2082, 2095, 2147, 2164, 2199, 2213, 2227, 2233, 2246, 2325, 2330, 2426, 2448, 2453, 2478, 2486, 2493, 2538, 2547, 2557, 2578, 2582, 2586, 2635, 2707, 2710, 2724, 2735, 2802, 2806, 2921, 2925, 2934, 3068, 3138, 3179, 3182, 3185, 3263, 3291, 3299, 3313, 3351, 3360, 3375, 3380, 3424, 3427, 3436, 3481, 3490, 3524, 3549, 3552, 3556, 3583, 3587, 3597, 3600, 3618, 3631, 3675, 3686, 3694, 3701, 3707, 3751, 3763, 3781, 3789, 3817, 3828, 3832, 3849, 3855, 3880, 3902, 3914, 3923, 3928, 3932, 3940, 3944, 3970, 4054, 4145, 4191, 4240, 4244, 4247, 4256, 4259, 4265, 4277, 4292, 4301, 4340, 4380, 4383, 4389, 4395, 4401, 4452, 4457, 4478, 4482, 4521, 4527, 4530, 4537, 4595], 'incorporation': [28, 268, 632], 'a': [30, 72, 159, 212, 225, 270, 312, 399, 452, 465, 594, 634, 638, 793, 855, 915, 973, 1225, 1300, 1522, 1671, 1687, 1764, 1839, 1898, 1913, 2071, 2139, 2144, 2169, 2342, 2394, 2418, 2466, 2483, 2515, 2527, 2575, 2624, 2648, 2750, 2822, 3000, 3010, 3082, 3123, 3199, 3256, 3268, 3321, 3343, 3502, 3611, 3648, 4134, 4173, 4237, 4271, 4356, 4411, 4581], 'mismatched': [31, 51, 187, 198, 271, 291, 427, 438, 609, 635, 708, 870, 929, 1008, 1766], 'nucleotide,': [32, 272, 722], 'primer': [34, 199, 274, 439, 640, 662, 709, 729, 998, 1029, 1065, 1185, 1304, 1331, 1432, 1497, 1503, 1523, 1604, 1635, 1848, 1896, 2411, 2682, 2811, 3525, 3588, 3740, 3803, 4253], 'terminus': [35, 275, 730, 1605, 4254], 'is': [36, 276, 591, 922, 1361, 1433, 1439, 1445, 1481, 1487, 1550, 1560, 1849, 3377, 3519, 3805, 4392, 4494], 'transferred': [37, 277], 'from': [38, 181, 200, 278, 421, 440, 710, 1307, 1525, 2119, 2186, 2208, 2239, 2564, 2936, 2962, 2973, 3024], 'site': [41, 45, 140, 281, 285, 380, 667, 701, 734, 784, 1313, 1376, 1438, 1531, 2073, 2229, 3382, 3429, 4294, 4387], 'to': [42, 158, 203, 282, 398, 443, 663, 713, 731, 770, 863, 969, 999, 1051, 1265, 1270, 1291, 1305, 1310, 1349, 1435, 1489, 1518, 1528, 1630, 1637, 1644, 1690, 1776, 1837, 1903, 2159, 2197, 2323, 2482, 2526, 2545, 2566, 2607, 2689, 2821, 2975, 3009, 3015, 3034, 3073, 3117, 3340, 3511, 3580, 3681, 3699, 3749, 3891, 3938, 4172, 4255, 4349], 'an': [43, 283, 1272, 1642, 2153, 2931, 3162], 'designed': [46, 286], 'excision': [48, 288, 718, 1005, 3589], 'nucleotides.': [52, 292], 'structural': [54, 294, 773], 'comparison': [55, 295, 4200, 4372], 'binary': [58, 298], 'complexes': [59, 299, 1989, 3070, 3158], 'polymerases': [62, 302, 675, 787, 990, 1060, 3370], 'in': [63, 80, 86, 97, 162, 196, 224, 303, 320, 326, 337, 402, 436, 464, 658, 759, 820, 858, 908, 912, 925, 993, 1177, 1180, 1344, 1797, 1938, 2033, 2051, 2146, 2173, 2341, 2417, 2475, 2647, 2837, 3081, 3122, 3181, 3267, 3346, 3350, 3379, 3623, 3697, 3706, 3807, 3827, 3901, 3931, 3984, 4007, 4030, 4068, 4089, 4109, 4133, 4199, 4243, 4276, 4300, 4359, 4371, 4388, 4394, 4414, 4451, 4496, 4520, 4556, 4569, 4586, 4594], 'modes,': [69, 309], 'together': [70, 310, 3297, 3646], 'with': [71, 153, 186, 311, 393, 426, 611, 756, 799, 833, 1369, 1692, 1757, 1763, 1781, 2195, 2409, 2560, 2593, 2860, 2886, 2957, 3241, 3298, 3555, 3582, 3647, 3691, 3769, 3991, 4014, 4197, 4201, 4212, 4268, 4373, 4422, 4489, 4580], 'molecular': [73, 313, 1663, 3561], 'modeling': [74, 314, 1664, 1833, 3562], 'template': [77, 222, 317, 462, 1355, 1385, 1428, 1502, 1625, 1655, 1694, 1803, 1844, 1911, 1925, 1936, 2075, 2396, 2691, 3357, 3493, 3515, 3553, 3584, 4248, 4298, 4384], 'strand': [78, 318, 641, 1030, 1356, 1498, 1636, 1656, 1845, 1937, 3526, 3554, 3585], 'overhang': [79, 319, 1386, 1429, 1804, 1926, 2076, 3358, 3494, 4299, 4385], 'fragment,': [82, 322], 'indicated': [83, 323], 'its': [84, 324, 612, 1034, 1478, 3512, 4360], 'binding': [85, 165, 213, 229, 325, 405, 453, 469, 830, 1387, 1474, 1799, 1811, 2077, 2951, 3352, 4245, 4278, 4386], 'region': [88, 107, 328, 347, 3345, 3505, 3609], 'spanning': [89, 329], 'residues': [90, 94, 119, 330, 334, 359, 1021, 1172, 1649, 1737, 2066, 2088, 3567, 3595, 3915, 4275], '821–824.': [91, 331], 'Since': [92, 332, 3897, 4233], 'these': [93, 168, 173, 333, 408, 413, 743, 951, 1787, 2087, 3594, 3885, 4274, 4514], 'conserved': [96, 336, 1672, 2091], '"A"': [99, 339], 'family': [100, 340, 1975, 2098, 3603], 'polymerases,': [102, 342], 'we': [103, 343, 1660, 1789, 1858, 1968, 3558, 3614, 4234, 4338], 'have': [104, 344, 1048, 1165, 1380, 3371, 3430, 3615], 'designated': [105, 345, 1676], 'this': [106, 121, 346, 361, 1345, 1359, 1699, 1856, 1966, 3608, 3628, 3704, 3755, 4352], 'as': [108, 348, 1493, 1620, 1677, 1686, 2069, 2514, 2574, 2767, 2778, 2914, 2978, 2999, 3041, 3215, 3332, 3610, 3617, 3658, 3852, 3946, 3961, 4291], 'RRRY': [110, 209, 350, 449, 1679, 1739, 1795, 1818, 3619, 3917, 4241], 'motif.': [111, 351, 3620], 'alanine': [113, 353, 1728, 3635, 3929], 'substitution': [114, 354, 1218, 3930], 'individual': [116, 356, 2550, 3638, 3770, 4538], 'amino': [117, 357, 816, 1019, 1170, 1337, 1647, 1673, 1735, 3639], 'acid': [118, 358, 817, 1020, 1171, 1338, 1648, 1674, 1736], 'motif': [122, 210, 362, 450, 1680, 1700, 1740, 1796, 3629, 3705, 3918, 4242], 'did': [123, 363, 1741], 'not': [124, 364, 746, 1440, 1483, 1596, 1742, 1928, 3507, 3920, 3936, 4049, 4129], 'change': [125, 365, 3826, 3900], 'activity;': [128, 368], 'however,': [129, 369, 921], 'activity': [133, 373, 615, 1230, 1235, 1298, 1750, 2328, 2338, 2389, 2611, 2644, 2923, 3718, 3830, 3851, 3874, 3904, 3968, 3990, 3994, 4013, 4017, 4039, 4052, 4076, 4096, 4118, 4143, 4164, 4196, 4209, 4228, 4263, 4342, 4403, 4596], 'was': [134, 175, 374, 415, 1267, 1285, 1701, 1724, 1754, 1760, 1834, 1961, 2157, 2193, 2258, 2321, 2339, 2390, 2480, 2512, 2640, 2645, 2764, 2812, 2858, 2884, 3038, 3190, 3212, 3338, 3731, 3747, 3767, 3778, 3888, 3908, 3911, 4150, 4169, 4210, 4435, 4540, 4577], 'reduced': [135, 375, 1228, 1756], '2–29-fold,': [136, 376], 'depending': [137, 377], 'upon': [138, 378], 'mutation.': [142, 382], 'R821A': [144, 384, 1771, 4213, 4460, 4505, 4563, 4575], 'R822A/Y824A': [146, 386, 1773, 2257, 3862, 4507], 'mutant': [147, 192, 387, 432, 1721, 1752, 1769, 2115, 2250, 2333, 2382, 2638, 2717, 2876, 2899, 2928, 3109, 3247, 3650, 3687, 3723, 3820, 3833, 3842, 3863, 3886, 3906, 3933, 3958, 4148, 4167, 4184, 4216, 4266, 4345, 4353, 4365, 4419, 4438, 4445, 4485, 4508, 4515, 4547, 4564], 'enzymes': [148, 174, 193, 388, 414, 433, 1753, 1770, 2116, 2251, 2639, 2929, 3688, 3724, 3771, 3821, 3834, 3864, 3887, 3907, 3934, 3959, 4149, 4168, 4185, 4267, 4439, 4461, 4486, 4509, 4516, 4565], 'showed': [149, 389, 1892, 2085, 3865, 4186, 4193, 4367, 4409, 4566], 'maximum': [150, 390, 4449], 'cleavage': [151, 232, 391, 472, 1480, 2773, 2879, 2905, 2922, 4416, 4480, 4503, 4570, 4587], 'defect': [152, 392], 'single-stranded': [154, 221, 394, 461, 537, 586, 3356], 'DNA,': [155, 395, 967], 'mainly': [156, 396], 'due': [157, 397, 3510], 'large': [160, 400], 'decrease': [161, 401, 4450], 'ssDNA': [164, 217, 404, 457, 1452, 1476, 1758, 1801, 1813, 2594, 2614, 2629, 2672, 2775, 2840, 2857, 2904, 2945, 2954, 4230, 4269, 4348, 4464, 4491, 4502], 'affinity': [166, 406, 649, 3878], 'enzymes.': [169, 409, 1722, 2334, 2383, 2877, 3110, 4218], 'Mismatch': [170, 410, 2599], 'removal': [171, 411, 2804, 2908, 4433], 'only': [176, 416, 3420, 4222, 4410], 'moderately': [177, 417, 1761, 4553], 'affected.': [178, 418], 'However,': [179, 419, 749, 1423, 1470, 1747, 1920, 3499], 'data': [180, 420, 2523, 2817, 3006], 'exonuclease-polymerase': [183, 423], 'balance': [184, 424, 3183], 'assays': [185, 425], 'template-primer': [188, 428, 826, 2385, 2890, 3197, 3376, 3735, 3760], 'suggest': [189, 429], 'defective': [195, 435], 'switching': [197, 437, 1062], 'site.': [206, 239, 446, 479, 1609, 1640, 1854], 'Thus,': [207, 447, 670, 1010, 1217, 1610], 'provides': [211, 451, 767], 'track': [214, 454], 'substrate': [216, 235, 456, 475, 3881], 'nonsubstrate': [220, 460], 'overhang,': [223, 463, 4249], 'polarity-dependent': [226, 466], 'manner.': [227, 467], 'This': [228, 468, 656, 919, 978, 1890, 2133, 3210], 'then': [230, 470, 2572, 3115, 3653], 'facilitates': [231, 471], 'at': [236, 476, 686, 698, 1606, 1851, 2226, 2436, 2443, 2706, 2742, 2937, 2968, 3025, 3078, 3127, 3149, 3252, 3306, 3426, 3762, 3772, 3987, 4010, 4033, 4071, 4092, 4112], 'faithful': [481], 'synthesis': [482, 645], 'E.': [486, 499, 518, 548, 567, 3624], '(pol': [491, 702], 'I)': [492, 1685], '2The': [493, 542], 'abbreviations': [494, 543], 'used': [495, 544, 1661, 1969, 2322, 2386, 2606, 3559, 3748, 4393], 'are:': [496, 545], 'pol': [497, 509, 546, 558, 712, 733, 1069, 1189, 1312, 1342, 1449, 1527, 1684, 2096, 3347, 3381, 3570, 3601, 3626, 4286], 'I,': [498, 547, 3627], 'I;': [503, 522, 532, 552, 571, 581], 'exo': [504, 553, 715, 1001, 1072, 1191, 1309, 1437, 1530, 1608, 1639, 1853, 1905, 3428], 'site,': [505, 510, 554, 559, 716, 1002, 1906, 4258], 'active': [507, 512, 556, 561, 666, 783, 1558], 'site;': [508, 513, 557, 562], 'KF,': [514, 563, 3501], 'Klentaq,': [523, 572], 'equivalent': [526, 575], 'T.': [528, 577, 619, 837, 934, 1458, 3392], 'aquaticus': [529, 578, 1866], 'WT,': [533, 582], 'wild': [534, 583], 'type;': [535, 584], 'ssDNA,': [536, 585, 4198, 4558], 'DNA;': [538, 587], 'dsDNA,': [539, 588], 'double-stranded': [540, 589, 2031], 'DNA.': [541, 590], 'accomplished': [592], 'combination': [595], 'two': [597, 902, 952, 1038, 1557, 2939, 3027, 3207, 3300, 3421, 3744, 3791, 3841], 'processes:': [598], '(a)': [599, 692], 'correctly': [600], 'incorporating': [601], 'matched': [602, 867, 899], 'nucleotides': [603, 610, 3746, 4000, 4023, 4082, 4102, 4534], '(b)': [605, 704], 'removing': [606], 'incorrectly': [607], 'incorporated': [608, 2511, 3815, 3978, 4062], '(1Kornberg': [616, 1455], 'A.': [617, 879, 886, 1131, 1393, 1456, 2279, 4307], 'Baker': [618, 1457], 'Replication.': [621, 1460], 'W.': [622, 1461], 'H.': [623, 1462], 'Freeman': [624, 1463], 'Co.,': [626, 1465], 'New': [627, 1466], 'York1992:': [628, 1467], '130Google': [629, 1468], 'Scholar).': [630, 849, 894, 946, 1163, 1216, 1259, 1326, 1422, 1469, 1546, 1582, 2027, 2312, 2799, 2993, 3066, 3417, 3479, 4336], 'nucleotide': [636, 697, 737, 2911], 'on': [637, 1786, 2048, 2163, 2465, 2749, 3161, 3320, 4229, 4347, 4404, 4431], 'growing': [639], 'stalls': [642], 'further': [643, 736, 3606], 'decreases': [647], 'template-primer.': [655, 1767], 'results': [657], 'shuttling': [659, 995, 1332, 1632, 4251], '(exo': [668], 'site).': [669], 'error': [671], 'correction': [672], 'contain': [677], 'involves': [685], 'least': [687, 2938, 3026], 'following': [689], 'four': [690, 1734], 'steps:': [691], 'sensing': [693, 1057], 'misincorporated': [696], 'site),': [703], 'transfer': [705, 725, 1306, 1520], '(c)': [717], 'incorrect': [721], '(d)': [724], 'corrected': [728], 'incorporation.': [738, 2542], 'mechanistic': [740], 'details': [741], 'steps': [744], 'fully': [747], 'understood.': [748], 'availability': [751], 'structures,': [755, 1978], 'bound': [758, 1434, 1850, 3378], 'either': [760], 'or': [763, 972, 2381, 2630, 2637, 2679, 2716, 2875, 2898, 3108, 3824, 4428], 'mode,': [766], 'some': [768], 'clues': [769], 'possible': [772], 'alterations': [774], 'protein': [778, 835, 963, 1379, 1616, 3141], 'during': [779, 1358, 1388, 1477, 1657, 1805, 3359, 3586], 'synthesis.': [781], 'catalyzing': [788], 'reaction': [791, 1391, 2344, 2650, 3084, 3112, 3259, 3777], 'has': [792, 807, 1506, 3630, 4288], 'configuration': [794], 'allows': [796], 'non-sequence-specific': [797], 'contacts': [798], 'incoming': [804, 2375, 3208, 3745, 3792], 'dNTP.': [805], 'It': [806, 1505, 1723], 'been': [808, 1049, 1508, 3372, 3431, 4289], 'suggested': [809], 'hydrogen-bonding': [812], 'interactions': [813, 851, 961, 3491, 3550], 'certain': [815], 'side': [818, 964, 1281, 1339], 'chains': [819, 965, 1340], 'minor': [822], 'groove': [823], 'govern': [827, 1033], 'sequence-independent': [829], '(2Brown': [836, 933], 'Kennard': [838, 935], 'O.': [839, 936], 'Curr.': [840, 937], 'Biol.': [841, 938, 1077, 1093, 1112, 1149, 1202, 1245, 2298, 3052, 3230], '1992;': [842, 939], '2:': [843, 940], '299Abstract': [844, 941], 'Full': [845, 942, 1082, 1098, 1154, 1156, 1207, 1209, 1250, 1252, 2000, 2002, 2018, 2020, 2303, 2305, 3057, 3059, 3235, 3451, 3453], 'Text': [846, 943, 1083, 1099, 1155, 1157, 1208, 1210, 1251, 1253, 2001, 2003, 2019, 2021, 2304, 2306, 3058, 3060, 3236, 3452, 3454], 'PDF': [847, 944, 1084, 1100, 1158, 1211, 1254, 2004, 2022, 2307, 3061, 3237, 3455], 'Google': [848, 893, 945, 1086, 1102, 1119, 1138, 1162, 1215, 1258, 1325, 1405, 1421, 1545, 1581, 1959, 2008, 2026, 2276, 2291, 2311, 2798, 2992, 3065, 3239, 3400, 3416, 3459, 3478, 3540, 3672, 4319, 4335], 'These': [850, 3433, 4219], 'most': [852, 957, 1779, 1820, 4205], 'likely': [853, 958, 1821, 3579], 'play': [854], 'primary': [856], 'role': [857, 1697, 3702, 4238, 4272], 'ability': [860], 'distinguish': [864], 'between': [865, 962, 1036, 1067, 1187, 1555], 'base': [872, 900, 931, 1515, 1589], 'pairs': [873, 932, 1516, 1590], '(3Seeman': [874], 'N.C.': [875], 'Rosenberg': [876], 'J.M.': [877], 'Rich': [878], 'Proc.': [880, 1125], 'Natl.': [881, 1126], 'Acad.': [882, 1127], 'Sci.': [883, 1128], 'U.': [884, 1129], 'S.': [885, 1130, 1141, 1194, 1237, 3384, 3386, 3405], '1976;': [887], '73:': [888], '804-808Crossref': [889], 'PubMed': [890, 1085, 1101, 1116, 1135, 1159, 1212, 1255, 1322, 1402, 1418, 1542, 1578, 1956, 2005, 2023, 2273, 2288, 2308, 2795, 2989, 3062, 3238, 3397, 3413, 3456, 3475, 3537, 3669, 4316, 4332], 'Scopus': [891, 1117, 1136, 1160, 1213, 1256, 1323, 1403, 1419, 1543, 1579, 1957, 2006, 2024, 2274, 2289, 2309, 2796, 2990, 3063, 3398, 3414, 3457, 3476, 3538, 3670, 4317, 4333], '(938)': [892], 'In': [895, 1442, 1547, 1641, 2080, 2101, 2543, 3418, 3480, 3754, 3926], 'case': [897, 927], 'pairs,': [901], 'hydrogen': [903, 976], 'bond': [904], 'accepting': [905], 'groups': [906], '(N3': [907, 953], 'purines': [909], 'O2': [911], 'pyrimidines)': [913], 'occupy': [914], 'topologically': [916], 'similar': [917, 3503, 4357], 'position.': [918], 'position,': [920], 'clearly': [923], 'different': [924, 2969], 'altered': [948, 1276], 'position': [949, 1383, 1425, 1841, 1922], 'O2)': [955], 'atoms': [956], 'perturbs': [959], 'leading': [968], 'steric': [970], 'hindrance': [971], 'loss': [974, 4207, 4260], 'bond(s).': [977], 'mechanism': [979, 1329], 'may': [980, 1013, 1595, 1627], 'be': [981, 1271, 1490, 1628, 1777, 1929, 3496, 3508, 3892], 'one': [982, 2704, 2882, 2958, 3980, 4064, 4407], 'several': [985, 1046], 'mispair-sensing': [986], 'mechanisms': [987, 1054], 'utilized': [988], 'result': [992], 'followed': [1003, 1708, 3146, 4455, 4589], 'nucleotide.': [1009], 'efficient': [1011], 'proofreading': [1012], 'require': [1014], 'presence': [1016], 'specific': [1018, 2326, 3344, 3954], 'within': [1022, 1377, 1614], 'domain,': [1025], 'which': [1026, 1979, 2142, 3577, 3613], 'bind': [1027, 1651], 'also': [1032, 1482, 1507, 2237, 3889], 'partitioning': [1035, 1182], 'sites.': [1039], 'Using': [1040], 'KF': [1041, 1370, 1511, 1881, 1943, 2053, 2068, 2136, 3464, 3721, 4227], 'T4': [1043], 'polymerase,': [1045, 3489], 'attempts': [1047], 'made': [1050], 'understand': [1052], 'mismatch': [1056, 1658, 2705, 2803, 2883, 2893, 2907, 2959, 3201, 3363, 4408, 4415, 4432], 'moiety': [1066, 1186, 1524], 'sites': [1073, 1192, 1559], '(4Joyce': [1074], 'C.M.': [1075, 1413, 2789, 4327], 'J.': [1076, 1092, 1110, 1148, 1201, 1244, 1993, 2297, 3051, 3222, 3226, 3229, 3409], 'Chem.': [1078, 1094, 1150, 1203, 1246, 2299, 3053, 3231], '1989;': [1079], '264:': [1080], '10858-10866Abstract': [1081], 'Scholar,': [1087, 1103, 1120, 1139, 1406, 2009, 2277, 2292, 3401, 4320], '5Spacciapoli': [1088], 'P.': [1089, 1567], 'Nossal': [1090], 'N.G.': [1091, 1571], '1994;': [1095, 3232], '269:': [1096, 3233], '438-446Abstract': [1097], '6Stocki': [1104], 'S.A.': [1105], 'Nonay': [1106], 'R.L.': [1107], 'Reha-Krantz': [1108, 1123], 'L.J.': [1109, 1124], 'Mol.': [1111], '1995;': [1113, 2792], '254:': [1114], '15-28Crossref': [1115], '(81)': [1118], '7Baker': [1121], 'R.P.': [1122], '1998;': [1132, 3394, 3410], '95:': [1133], '3507-3512Crossref': [1134], '(49)': [1137], '8Tuske': [1140], 'Singh': [1142, 1195, 1238, 1394, 2280, 3044, 4308], 'K.': [1143, 1196, 1239, 1315, 1395, 2266, 2281, 2294, 2982, 3048, 3662, 4309], 'Kaushik': [1144, 1197, 1240], 'N.': [1145, 1198, 1241], 'Modak': [1146, 1199, 1242, 1316, 1396, 2267, 2282, 2295, 2983, 3046, 3049, 3663, 4310], 'M.J.': [1147, 1200, 1243, 1317, 1397, 2268, 2283, 2296, 2984, 3050, 3664, 4311], '2000;': [1151, 1204, 1247], '275:': [1152, 1205, 1248], '23759-23768Abstract': [1153, 1206, 1249], '(17)': [1161, 1214, 1257], 'We': [1164, 1669, 2058], 'previously': [1166, 2264, 3660], 'demonstrated': [1167], 'J-helix': [1175], '(Gln677': [1176], 'particular)': [1178], 'participate': [1179], '(8Tuske': [1193, 1236], 'C-terminal': [1221], 'proline': [1222], '(Pro680)': [1223], 'glycine': [1226], 'significantly': [1227, 1755], 'concurrently': [1232], 'increased': [1233, 1295, 3867], 'effect': [1261, 1274, 4430], 'mutating': [1263], 'Pro680': [1264], 'Gly': [1266], 'eventually': [1268], 'interpreted': [1269], 'indirect': [1273], 'conformation': [1277], 'Gln677': [1280, 1290], 'chain': [1282], 'when': [1283, 1430, 1846], 'it': [1284, 1444, 1486, 1549, 3910], 'found': [1286, 1775, 3890], 'mutation': [1288, 2225], 'Ala': [1292], 'caused': [1293], 'failure': [1301], '(9Singh': [1314, 2265, 2981, 3661], 'Biochemistry.': [1318, 1398, 1414, 1538, 2269, 2284, 2985, 3665, 4312, 4328], '2005;': [1319, 2270, 2986, 3666], '44:': [1320, 2271, 2987, 3667], '8101-8110Crossref': [1321, 2272, 2988, 3668], '(6)': [1324, 1404, 2275, 2290, 2991, 3671, 4318], 'Although': [1327], 'involvement': [1335, 1792], 'process': [1346, 1360, 3361], 'beginning': [1348], 'unfold,': [1350], 'status': [1352], 'stabilization': [1357, 3354], 'still': [1362], 'unclear.': [1363], 'UV-mediated': [1365, 3030, 3036], 'cross-linking': [1366, 1375, 3037], 'identification': [1372], 'revealed': [1381], '(10Srivastava': [1392, 4306], '2003;': [1399, 1415, 2285, 2300, 3054, 4313, 4329], '42:': [1400, 1416, 2286, 4314, 4330], '3645-3654Crossref': [1401, 2287, 4315], '11Turner': [1407, 4321], 'Jr.,': [1408, 4322], 'R.M.': [1409, 4323], 'Grindley': [1410, 4324], 'N.D.': [1411, 4325], 'Joyce': [1412, 2126, 2788, 4326], '2373-2385Crossref': [1417, 4331], '(43)': [1420, 4334], 'known.': [1441], 'addition,': [1443, 1548, 2081, 3927], 'well': [1446], 'established': [1447, 1509], 'cleaves': [1451], 'quite': [1453, 4518], 'efficiently': [1454], 'mode': [1472, 1872, 1883, 1988, 2036, 3483, 4391], 'exonucleolytic': [1479, 2965, 4479], 'known,': [1484], 'although': [1485, 1894], 'presumed': [1488], 'same': [1492, 3850], 'mismatch-containing': [1501, 2632], 'duplex.': [1504], 'melts': [1512], '4': [1514, 1587, 3079], 'prior': [1517], '(12Catalano': [1532], 'C.E.': [1533], 'Allen': [1534], 'D.J.': [1535], 'Benkovic': [1536], 'S.J.': [1537], '1990;': [1539], '29:': [1540], '3612-3621Crossref': [1541], '(54)': [1544], 'known': [1551], 'distance': [1554, 1899], 'around': [1561], '30–35': [1562], 'Å': [1563, 1902, 1917], '(13Ollis': [1564], 'D.L.': [1565], 'Brick': [1566], 'Hamlin': [1568], 'R.': [1569], 'Xuong': [1570], 'Steitz': [1572, 1950, 1994, 2012, 3445, 3469, 3531], 'T.A.': [1573, 1951, 1995, 2013, 3446, 3470, 3532], 'Nature.': [1574, 3393], '1985;': [1575], '313:': [1576], '762-766Crossref': [1577], '(735)': [1580], 'Therefore,': [1583, 4337], 'mere': [1584], 'melting': [1585], 'terminal': [1588, 2892, 2910, 3200], 'duplex': [1593], 'suffice': [1597], 'successful': [1600], 'localization': [1601], 'other': [1611], 'conformational': [1612], 'adjustments': [1613], 'and/or': [1617, 3353], '(such': [1619], 'repositioning': [1622], 'strand)': [1626], 'necessary': [1629], 'facilitate': [1631], 'attempt': [1643], 'identify': [1645], '(or': [1652, 4279], 'stabilize)': [1653], 'removal,': [1659], 'comparative': [1662, 3560], 'sequence': [1666, 1675, 2083, 3632], 'alignment': [1667, 2084], 'techniques.': [1668], 'identified': [1670, 2059, 3564, 4290], '(residues': [1681], '821–824': [1682], 'potential': [1688, 2072, 3565], 'candidate': [1689], 'interact': [1691, 3581], 'strand.': [1695, 2203], 'assessed': [1702, 2340, 2646, 3732], 'means': [1704, 2827], 'site-directed': [1706, 2176, 3656], 'mutagenesis,': [1707, 3657], 'biochemical': [1711], 'characterization': [1712], 'observed': [1725], 'substitutions': [1729], 'any': [1731], 'alter': [1743], 'activity.': [1746, 2150, 3753, 4362], 'affected': [1762, 1780], 'terminally': [1765], 'were': [1774, 2044, 2117, 2166, 2206, 2216, 2230, 2423, 2440, 2450, 2463, 2489, 2501, 2524, 2554, 2571, 2589, 2604, 2721, 2747, 2776, 2819, 2912, 2960, 2997, 3007, 3071, 3114, 3135, 3159, 3169, 3250, 3288, 3304, 3318, 3330, 3652, 3679, 3689, 4552], 'all': [1782, 4038, 4117, 4166], 'substrates.': [1784], 'Based': [1785], 'results,': [1788], 'propose': [1790], 'reaction.': [1808, 3590], 'Furthermore,': [1809], 'requires': [1814], 'participation': [1816], 'motif,': [1819, 3612], 'recognizing': [1823], 'polarity': [1825], 'element': [1826], 'substrate.': [1830], 'Molecular': [1831], 'Modeling—Molecular': [1832], 'carried': [1835, 2259, 2765, 3039, 3191], 'out': [1836, 2260, 2442, 2766, 3040, 3192, 4379], 'determine': [1838, 2324, 2546, 3016, 3341], 'tentative': [1840], 'For': [1855, 2620], 'purpose,': [1857], 'first': [1859, 2605], 'superposed': [1860, 2047], 'crystal': [1862, 1977, 2042, 3366], 'structure': [1863, 1940, 2043, 3438, 3485, 3942], 'Thermus': [1865], '(KlenTaq': [1870], 'complex;': [1873, 1884], 'Protein': [1874, 1885], 'Data': [1875, 1886, 3543], 'Bank': [1876, 1887, 3544], 'file': [1877, 1888, 3545], '3ktq)': [1878], 'onto': [1879], '(exonuclease': [1882], '1kln).': [1889, 3546], 'superposition': [1891], 'moves': [1897], '∼35': [1901], '5′-end': [1908, 3764], 'shows': [1912, 3822], 'displacement': [1914], '∼20': [1916], '(Fig.': [1918, 2056, 2078, 2099, 3572, 3604, 3835, 4176, 4510, 4544], '1B).': [1919, 2057, 2079], 'could': [1927, 3506], 'inferred,': [1930], 'because': [1931], 'length': [1933, 3518], 'editing': [1944, 2035, 2054], 'complex': [1945, 2037, 2055, 4305], '(14Beese': [1946, 3465, 3527], 'L.S.': [1947, 3466, 3528], 'Derbyshire': [1948, 2781, 3467, 3529], 'V.': [1949, 2785, 3468, 3530], 'Science.': [1952, 2189, 3471, 3533], '1993;': [1953, 3472, 3534], '260:': [1954, 3473, 3535], '352-355Crossref': [1955, 3474, 3536], '(448)': [1958, 3477, 3539], 'Scholar)': [1960, 3460, 3541], 'too': [1962], 'short.': [1963], 'To': [1964, 2151, 2800, 3547, 4525], 'overcome': [1965], 'problem,': [1967], 'RB69': [1971, 2039, 3440, 3487], '(a': [1973], 'B': [1974], 'polymerase)': [1976], 'available': [1981], '(15Franklin': [1990], 'M.C.': [1991], 'Wang': [1992], 'Cell.': [1996, 2014, 3447], '2001;': [1997], '105:': [1998], '657-667Abstract': [1999], '(485)': [2007], '16Shamoo': [2010], 'Y.': [2011, 3403, 3444], '1999;': [2015, 3448], '99:': [2016, 3449], '155-166Abstract': [2017, 3450], '(321)': [2025, 3458], 'coordinates': [2029], 'extracted': [2045], 'Arg821,': [2061], 'Arg822,': [2062], 'Leu823,': [2063], 'Tyr824': [2065], 'forming': [2070], 'highly': [2090], 'among': [2092], 'members': [2093, 3598], '1C).': [2100], 'Vitro': [2102], 'Site-directed': [2103], 'Mutagenesis,': [2104], 'Expression,': [2105], 'Purification': [2107], 'Wild': [2109], 'Type': [2110], '(WT)': [2111], 'Mutant': [2113, 2204, 2848, 4470], 'Enzymes—The': [2114], 'generated': [2118, 2391, 3654], 'plasmid': [2120, 2134], '(pCJ141)': [2121], 'generously': [2122], 'provided': [2123, 2537], 'C.': [2125, 2240], 'Yale': [2128], 'University': [2129], '(New': [2130], 'Haven,': [2131], 'CT).': [2132], 'encodes': [2135], 'contains': [2138], 'D424A': [2140], 'mutation,': [2141], 'confers': [2143], 'deficiency': [2145, 4358, 4413], 'obtain': [2152], 'exonuclease-efficient': [2154], 'enzyme,': [2155, 3557, 3696, 3857, 3945], 'Ala424': [2156], 'reverted': [2158], 'Asp424.': [2160], 'Desired': [2161], 'mutations': [2162, 2215, 3636], 'plasmids': [2165, 2205, 2222], 'introduced': [2167], 'PCR-based': [2170], 'protocol': [2171], 'described': [2172, 2263, 2768, 2779, 2915, 2979, 3042, 3216, 3333], "Stratagene's": [2174], 'QuikChange': [2175], 'mutagenesis': [2177], 'kit,': [2178], 'using': [2179, 2530, 2581, 2832, 3012, 3193, 3743, 4155], 'pfu-turbo': [2180], 'PCR-grade': [2183], 'dNTPs': [2184, 2510, 3793], 'obtained': [2185, 2238, 2555, 2967], 'Roche': [2187], 'Applied': [2188], 'PCR': [2191], 'product': [2192], 'treated': [2194], 'DpnI': [2196], 'digest': [2198], 'methylated': [2200], 'non-mutated': [2201], 'parental': [2202], 'isolated': [2207], 'maintenance': [2209], 'strain': [2210, 2235], 'CJ406,': [2211], 'desired': [2214, 2228, 2743, 3307], 'confirmed': [2217, 2223], 'sequencing.': [2220], 'transformed': [2231], 'into': [2232], 'expression': [2234], 'CJ376': [2236], 'Joyce.': [2241], 'Expression': [2242], 'purification': [2244], 'WT': [2247, 2331, 2380, 2636, 2715, 2846, 2874, 2897, 2926, 3107, 3245, 3695, 3720, 3818, 3856, 3964, 3971, 3996, 4019, 4055, 4078, 4098, 4146, 4202, 4226, 4374, 4468, 4483], 'R821A,': [2252, 3859], 'R822A,': [2253, 3642, 3726, 4551], 'L823A,': [2254, 3643, 3727, 3846], 'Y824A,': [2255, 3728, 3860, 4560], 'methods': [2262], '10Srivastava': [2278], '17Singh': [2293], '278:': [2301, 3055], '11289-11302Abstract': [2302, 3056], '(26)': [2310, 3064], 'Specific': [2313], 'Polymerase': [2314, 3712], 'Activity': [2315, 2592, 3713, 4138, 4466], 'under': [2316, 2600, 2850, 3714, 3733, 4046, 4125, 4139, 4152, 4472], 'Steady-state': [2317, 2601, 3715, 4140], 'Conditions—Primer': [2318], 'extension': [2319, 2461, 3741, 3776, 3804], 'assay': [2320, 3211, 3742], 'standard': [2343, 2649, 3269], 'mixture': [2345, 2651], 'containing': [2346, 2617, 2652, 2891, 3086, 3144, 3166, 3198, 3272, 3325, 3423, 4406], '50': [2347, 2368, 2653, 3087, 3203, 3273, 3785], 'mm': [2348, 2353, 2360, 2430, 2654, 2659, 2666, 2729, 3088, 3093, 3096, 3274, 3279, 3286, 3295, 3796], 'Tris-HCl': [2349, 2655, 3275], '(pH': [2350, 2656, 3276], '7.8),': [2351, 2657, 3277], '1': [2352, 2618, 2631, 2658, 2861, 2887, 3092, 3194, 3278, 3985, 4003, 4008, 4026, 4031, 4069, 4085, 4090, 4105, 4110], 'dithiothreitol,': [2354, 2660, 3094, 3280], '0.01%': [2355, 2661, 3281], 'bovine': [2356, 2662, 3282], 'serum': [2357, 2663, 3283], 'albumin,': [2358, 2664, 3284], '0.2': [2359, 2665, 3285], 'EDTA,': [2361, 2667, 3097, 3287], '1.5': [2362, 3757], 'μm': [2363, 2369, 3204, 3758, 3786], '32/14-mer': [2364], 'template-32P-5′-end': [2365], 'labeled': [2366], 'primer,': [2367, 2711], 'each': [2370, 2520, 3205, 3787], 'dATP': [2371], 'dCTP': [2373], '(two': [2374], 'nucleotides),': [2376], '7.5': [2378, 3773], 'nm': [2379, 2669, 2714, 2862, 2873, 2888, 2896, 3195, 4157, 4161], 'annealing': [2393], '32-mer': [2395], '(5′-TGC': [2397, 2692], 'GCG': [2398, 2693], 'TTA': [2399, 2694], 'TAC': [2400, 2695], 'CGC': [2401], 'AGT': [2402], 'CGG': [2403], 'AG': [2404], 'T': [2405], 'GGC': [2406, 2696], 'TAA': [2407], 'CG-3′)': [2408, 2416, 2687], '14-mer': [2410, 2681, 2810], '(5′-CGT': [2412, 2673, 2683, 2865], 'TAG': [2413, 2674, 2684, 2866], 'CCA': [2414, 2675, 2685, 2867], 'CTC': [2415, 2676, 2686, 2868], 'ratio': [2419], '2:1.': [2421], 'Reactions': [2422], 'initiated': [2424, 2722, 3289, 3779], 'addition': [2427, 2454, 2725, 2736, 3139, 3292, 3783], '6': [2429, 3294, 3795], 'MgCl2': [2431, 2727, 3797], '(final': [2432, 3798], 'concentration)': [2433, 2731], 'incubated': [2435, 3768], '25': [2437, 2974, 3988, 4011, 4034, 4072, 4093, 4113], '°C.': [2438, 4035, 4114], 'Aliquots': [2439], 'taken': [2441], 'various': [2444, 3957], 'time': [2445, 2518, 2744, 3308, 4475, 4543], 'points,': [2446], 'reactions': [2449, 2720, 3134, 3266], 'quenched': [2451, 2733], "Sanger's": [2456, 2738], 'gel': [2457, 2479, 2739, 3165, 3324], 'loading': [2458, 2740], 'dye.': [2459], 'products': [2462, 3311], 'resolved': [2464, 2748, 3160, 3319], '16%': [2467, 2751, 3322], 'polyacrylamide,': [2468, 2752], '8': [2469, 2753, 3326, 4022, 4101], 'm': [2470, 2754, 3327], 'urea': [2471, 2755, 3328], 'gel.': [2472, 2756], 'Here': [2473], 'subsequent': [2476, 2838], 'assays,': [2477], 'exposed': [2481, 3116], 'PhosphorImager,': [2484], 'radioactive': [2487, 2762], 'bands': [2488, 2763], 'visualized': [2490, 3170], 'scanning': [2492], 'PhosphorImager': [2494], 'plate': [2495], '(GE': [2496, 2505, 3173], 'Healthcare).': [2497, 2506, 3174], 'band': [2499], 'intensities': [2500], 'quantitated': [2502, 3331], 'ImageQuant': [2504], 'amount': [2508, 3812], 'plotted': [2513, 2573, 2813, 2998, 4541], 'function': [2516, 2576, 3001, 3709], 'enzyme.': [2521, 2718, 2900], 'fitted': [2525, 2820, 3008], 'straight': [2528], 'line': [2529], 'GraphPad': [2531, 2833, 3013], 'Prism': [2532, 2834, 3014], '(GraphPad': [2533], 'Inc.).': [2534], 'slopes': [2536], 'rate': [2539, 2622, 2855, 2880, 4417, 4434], 'dNTP': [2541, 2561, 2579, 3737, 3814, 3882, 3977, 4061], 'order': [2544, 3698], 'Km(dNTP)': [2548, 2587, 4042, 4121], 'enzymes,': [2551, 3843, 4420, 4548], 'rates': [2552, 2570, 2612, 2771, 2902, 2919, 2963, 2996, 4571], '(velocities)': [2553], 'above': [2558], 'procedure,': [2559], 'concentrations': [2562, 3249], 'varying': [2563, 4174], '0.1': [2565], '10': [2567, 2713, 4156], 'μm.': [2568], 'concentration': [2580], 'Lineweaver-Burk': [2583], 'plot,': [2584], 'values': [2588, 2935, 3021], 'calculated.': [2590], 'Exonuclease': [2591, 4137], 'dsDNA': [2596, 2616, 2633, 4405], 'Containing': [2597, 2948], 'One': [2598, 2949], 'Conditions—Steady-state': [2602], 'conditions': [2603], 'estimate': [2608, 3750, 4526], 'mismatch.': [2619], 'steady-state': [2621, 4153], 'determination,': [2623], '10-fold': [2625], 'molar': [2626], 'excess': [2627], 'over': [2634], 'used.': [2641], '100': [2668, 3150, 4160], '32P-5′-end-labeled': [2670, 2680, 3101], '16-mer': [2671, 2864, 4490], 'CAG': [2677, 2869], 'G-3′)': [2678, 2870], 'annealed': [2688], '33-mer': [2690], 'ACC': [2697], 'CTG': [2698], 'GAG': [2699], 'TGG': [2700], 'CTA': [2701], 'ACG-3′)': [2702], 'providing': [2703, 3255], '3′-end': [2708], '(6': [2728], 'final': [2730], 'dye': [2741], 'intervals.': [2745, 3309], 'Products': [2746], 'visualization': [2758], 'quantitation': [2760], 'above.': [2769, 2916, 3334], 'calculated': [2777, 2913, 2961], 'et': [2782, 3219], 'al.': [2783, 3220], '(18Derbyshire': [2784], 'Pinsonneault': [2786], 'J.K.': [2787], 'Methods': [2790], 'Enzymol.': [2791], '262:': [2793], '363-385Crossref': [2794], '(68)': [2797], 'calculate': [2801], 'rate,': [2805], 'remaining': [2807], 'fraction': [2808], 'against': [2814, 4542], 'time.': [2815], 'points': [2818], 'single': [2823, 3257], 'exponential': [2824], 'decay': [2825], 'nonlinear': [2829], 'regression': [2830], 'analysis,': [2831], '(here': [2835], 'experiments).': [2839], 'One-mismatch': [2842], 'Cleavage': [2843, 4465], 'Rates': [2844], 'Enzymes': [2849, 4471], 'Pre-steady-state': [2851, 4473], 'Conditions—The': [2852, 3716, 4141, 4474], 'degradation': [2854, 2966, 4522], 'determined': [2859, 2885, 4130, 4151, 4339], '32P-5′end-labeled': [2863], '125': [2872], '33/14-mer': [2889, 3759], '75': [2895, 3253], 'reported': [2918, 3019, 3373], 'average': [2932], 'independent': [2940, 3028], 'experiments.': [2941, 3029], 'KD(DNA)': [2942, 3020], 'Determination': [2943], 'Mismatch—DNA': [2950], 'affinities': [2952], 'enzyme': [2970, 3003, 3248, 3677, 3972, 3983, 3997, 4006, 4020, 4029, 4056, 4067, 4079, 4088, 4099, 4108, 4158, 4346, 4354, 4366, 4375, 4447, 4539, 4576], 'concentrations,': [2971], 'ranging': [2972], '300': [2976], 'nm,': [2977, 3254], 'before': [2980], 'concentration.': [3004], 'hyperbola': [3011], 'KD(DNA).': [3017], 'derived': [3023], 'Cross-linking': [3031], 'Enzyme': [3033], 'ssDNA—The': [3035], '(17Singh': [3047], 'Briefly,': [3067], 'enzyme-ssDNA': [3069], 'allowed': [3072], 'form': [3074], '5': [3076, 3153], 'min': [3077, 3986, 4009, 4032, 4070, 4091, 4111], '°C': [3080, 3151], '30-μl': [3083], 'volume': [3085], 'Tris-HCl,': [3089], 'pH': [3090], '7.8,': [3091], '2': [3095], '30': [3098, 3104, 3999, 4081], 'fmol': [3099], '14-mer,': [3102], 'pmol': [3105, 3975, 3981, 4004, 4027, 4059, 4065, 4086, 4106], 'mixtures': [3113], 'UV': [3118], 'light': [3119], '(254': [3120], 'nm)': [3121, 4488, 4493], 'Spectrolinker': [3124], '(Spectronic': [3125], 'Corp.)': [3126], '3700': [3128], 'microwatts/cm2': [3129], '3': [3131], 'min.': [3132, 3154], 'terminated': [3136], 'solubilization': [3142], 'medium': [3143], 'SDS,': [3145], 'heating': [3148], 'cross-linked': [3156], 'enzyme-DNA': [3157], '8%': [3163], 'polyacrylamide': [3164, 3323], 'SDS': [3167], 'phosphorimaging': [3172], 'Exonuclease-Polymerase': [3175], 'Balance': [3176], 'Assay—Determination': [3177], 'alteration': [3180], '33/14': [3196], 'dNTPs.': [3209], 'performed': [3213], 'essentially': [3214], 'Mendez': [3218], '(19Méndez': [3221], 'Blanco': [3223], 'L.': [3224], 'Lázaro': [3225], 'Salas': [3227], 'M.': [3228], '30030-30038Abstract': [3234], 'Scholar),': [3240, 3673], 'slight': [3242], 'modifications.': [3243], 'maintained': [3251], 'turnover': [3258], 'activities.': [3264], 'buffer': [3270], 'condition': [3271], 'MgCl2,': [3296], 'dNTPs,': [3301], 'aliquots': [3303], 'removed': [3305], 'present': [3336], 'investigation': [3337], 'undertaken': [3339], 'if': [3342, 4351], 'participates': [3349], 'excision.': [3364], 'Several': [3365], 'structures': [3367, 3422, 3434], 'where': [3374], '(20Doublie': [3383], 'Tabor': [3385], 'Long': [3387], 'A.M.': [3388], 'Richardson': [3389], 'C.C.': [3390], 'Ellenberger': [3391], '391:': [3395], '251-258Crossref': [3396], '(1093)': [3399], '21Li': [3402], 'Korolev': [3404], 'Waksman': [3406], 'G.': [3407], 'EMBO': [3408], '17:': [3411], '7514-7525Crossref': [3412], '(651)': [3415], 'contrast,': [3419], 'reported.': [3432], 'template-primer-bound': [3437], '(16Shamoo': [3443], 'DNA-bound': [3484], 'can': [3495], 'easily': [3497], 'noted.': [3498], 'interacting': [3504], 'discerned,': [3509], 'relatively': [3513, 3893], 'short': [3514], 'strand,': [3516], 'whose': [3517], 'less': [3520], 'than': [3521], '(Protein': [3542], 'infer': [3548], 'motif-spanning': [3566], 'Arg821–Tyr824': [3568], '1,': [3573], 'A': [3574, 3602, 3800], 'B),': [3576], 'seemed': [3578], 'conservation': [3592], 'across': [3596], '1C)': [3605], 'qualified': [3607], 'named': [3616], 'Note': [3621], 'RRLY.': [3633], 'acids': [3640], '(R821A,': [3641, 3725], 'Y824A)': [3645], 'double': [3649, 4215, 4444, 4598], '(R822A/Y824A)': [3651, 4217, 4446], 'detailed': [3659], 'expressed': [3676, 3960], 'proteins': [3678], 'purified': [3680], 'homogeneity.': [3682], 'properties': [3684], 'compared': [3690, 4421], 'decipher': [3700], 'KF.': [3711, 4203], 'R822A/Y824A)': [3730], 'saturating': [3734], 'concentrations.': [3738], 'assay,': [3756], '(32P-label': [3761], 'primer)': [3766], 'nm.': [3774], 'simultaneous': [3782], 'next': [3790], 'concentration).': [3799], 'profile': [3801], 'shown': [3806, 4495], 'Fig.': [3808, 4497], '2A.': [3809], 'computed': [3811], 'little': [3823], 'no': [3825, 3898, 4429], '2A': [3836], 'Table': [3838, 4179], '1).': [3839, 3876, 3896, 4180, 4232, 4463], 'R822A': [3844, 4181, 4440], 'had': [3847, 4221, 4235], 'approximately': [3848], 'whereas': [3858, 4559], 'slightly': [3866], '(19,': [3868], '25,': [3869], '36%,': [3871], 'respectively)': [3872], '(Table': [3875, 3895, 4231, 4376, 4462, 4572], '(Km(dNTP))': [3883], 'unchanged': [3894], 'significant': [3899, 4206, 4567], 'noted,': [3909], 'inferred': [3912], 'required': [3921], 'function.': [3925], 'does': [3935], 'seem': [3937], 'perturb': [3939], 'overall': [3941], 'judged': [3947], 'their': [3949], 'intact': [3950], 'activity.TABLE': [3952], '1Relative': [3953], 'percentage': [3962], 'KFEnzymeDNA': [3965], 'activitya100%': [3967], 'equals': [3973, 3998, 4021, 4057, 4080, 4100], '315': [3974, 4058], '°CKm(dNTP)Exonuclease': [3989], 'ssDNAb100%': [3992], 'cleaved': [4001, 4024, 4083, 4103, 4535], '°CExonuclease': [4012], 'one-mismatch': [4015], 'DNAc100%': [4016], 'Details': [4036, 4115], 'measurements': [4040, 4119], 'calculations': [4043, 4122], 'given': [4045, 4124], '"Experimental': [4047, 4126], 'Procedures"%μm%%WT1001.4100100R821A1191.5572R822A1053.73192L823A972.63185Y824A1252.02543R822A/Y824A1363.4416F771A70NDdND,': [4048], 'determined70124a': [4050], '100%': [4051, 4074, 4095], '°Cb': [4073], '°Cc': [4094], 'Procedures"d': [4127], 'ND,': [4128], 'Open': [4131], 'table': [4132], 'new': [4135], 'tab': [4136], 'conditions,': [4154], 'ssDNA.': [4162, 4282, 4426, 4524], 'affected,': [4170], 'albeit': [4171], 'degree': [4175], '2B': [4177], 'L823A': [4183, 4549], '∼31%': [4187], 'activity,': [4189, 4370, 4454, 4588], 'Y824A': [4192, 4458], '∼25%': [4194], 'noted': [4211, 4436], 'mutants': [4220], '4–5%': [4224], 'projected': [4236], 'after': [4250], 'suggests': [4270, 4512], 'positioning)': [4280], 'Previously,': [4283], 'Phe771': [4284], 'major': [4293], 'interaction': [4296], 'enzyme-DNA-dNTP': [4302], '(polymerase)': [4303], 'ternary': [4304], 'F771A': [4344, 4364], 'assess': [4350], 'exhibits': [4355], '∼70%': [4368], '1),': [4377], 'ruling': [4378], 'possibility': [4381], 'mode.': [4397], 'assessment': [4399], 'moderate': [4412], 'seen': [4424], 'Little': [4427], 'L823A.': [4442], 'displayed': [4448], 'course': [4476], '(125': [4487], '(1': [4492], '3A.': [4498], 'pattern': [4500], '3A)': [4511], 'compromised': [4519], 'rates,': [4529], 'total': [4531], 'number': [4532], '3B).': [4545], '(∼2–3-fold)': [4554], 'deficient': [4555], 'cleaving': [4557], 'R822A/Y824A,': [4561], 'reduction': [4568, 4582, 4593], '2).': [4573], 'maximally': [4578], 'deficient,': [4579], '29-fold': [4585], '17-fold': [4592], 'mut': [4599]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2017222444', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-15T06:07:39.760347', 'created_date': '2016-06-24'}