cDNA Cloning and Expression of Bovine UDP-N-Acetylglucosamine:α1,3-d-Mannoside β1,4-N-Acetylglucosaminyltransferase IV

Name: Work Video:
Duration: 3 min 30 s
Mari T. Minowa; Suguru Oguri; Aruto Yoshida; Tomoka Hara; Akihiro Iwamatsu; Hiroshi Ikenaga; Makoto Takeuchi
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3905, 3910, 3923, 3940, 3946, 3967, 3998, 4001, 4021, 4026, 4047, 4184, 4195, 4200, 4211, 4235, 4269, 4278, 4296, 4303, 4318, 4331, 4336, 4340, 4382, 4534, 4597, 4603, 4615, 4631, 4766, 4774, 4799, 4802, 4808, 4830, 4837, 4840, 4851, 4935, 4953, 4959, 5000, 5017, 5020, 5031, 5035, 5059, 5072, 5081, 5085, 5276, 5321, 5349, 5370, 5375, 5393, 5397, 5433, 5437, 5482, 5485, 5503, 5583, 5586, 5594, 5599, 5608, 5622, 5632, 5638, 5642, 5658, 5662, 5666, 5698, 5702, 5708, 5722, 5735, 5739], 'essential': [7, 198, 437, 4250, 5558], 'enzymes': [8, 199, 438, 1173, 5272, 5399, 5675, 5715], 'in': [9, 69, 136, 149, 170, 185, 200, 260, 327, 340, 361, 376, 439, 472, 496, 571, 611, 666, 671, 681, 1287, 1302, 1616, 1692, 1785, 2057, 2440, 2460, 2683, 2696, 2713, 2909, 2937, 2962, 3043, 3060, 3265, 3308, 3465, 3548, 3671, 3678, 3688, 3689, 3753, 3776, 3787, 3961, 4046, 4199, 4214, 4295, 4327, 4524, 4537, 4555, 4625, 4939, 4982, 5022, 5071, 5090, 5281, 5295, 5379, 5389, 5401, 5492, 5612, 5648], 'production': [11, 202], 'tri-': [13, 204], 'and': [14, 60, 158, 205, 251, 349, 503, 506, 670, 741, 799, 804, 1242, 1264, 1282, 1401, 1411, 1440, 1463, 1492, 1588, 1596, 1605, 1614, 1652, 1661, 1664, 1673, 1695, 1747, 1767, 1797, 1808, 1815, 2037, 2070, 2123, 2164, 2181, 2188, 2243, 2262, 2277, 2328, 2350, 2398, 2430, 2450, 2510, 2524, 2526, 2623, 2649, 2660, 2667, 2678, 2693, 2698, 2722, 2754, 2760, 2772, 2784, 2922, 2978, 2995, 3028, 3032, 3105, 3121, 3164, 3179, 3190, 3254, 3267, 3279, 3288, 3323, 3375, 3385, 3393, 3441, 3471, 3528, 3584, 3628, 3713, 3734, 3810, 3817, 3830, 3842, 3872, 3883, 4006, 4013, 4181, 4190, 4335, 4522, 4528, 4544, 4602, 4670, 4676, 4776, 4780, 4804, 4829, 4854, 4948, 4978, 4990, 4997, 5025, 5058, 5084, 5097, 5106, 5260, 5372, 5409, 5418, 5496, 5644], 'tetra-antennary': [15, 206, 470, 492, 599, 1177, 5561], 'Asn-linked': [16, 207, 447, 4240, 4387, 5489], 'sugar': [17, 208, 448, 587, 642, 1178, 4246, 5490, 5685], 'chains.': [18, 209, 449, 588], 'Recently,': [19, 210], 'we': [20, 211, 1253, 3154, 3397, 3781, 3796, 4291, 4922], 'have': [21, 80, 212, 271, 591, 673, 801, 810, 1273, 3742, 3889, 4523, 4636, 5108, 5257], 'successfully': [22, 213, 1240], 'purified': [23, 38, 111, 138, 214, 229, 302, 329, 803, 1241, 1261, 1295, 1508, 2339, 3133, 3151, 3544, 3560, 3706, 3911, 4134, 4185, 4236, 4341, 4633], 'GnT-IV': [24, 40, 64, 112, 150, 167, 178, 215, 231, 255, 303, 341, 358, 369, 468, 676, 1181, 1244, 1262, 1270, 1280, 1293, 1391, 2039, 2495, 2637, 2729, 2801, 2999, 3002, 3152, 3234, 3321, 3360, 3546, 3561, 3609, 3711, 3738, 3752, 3774, 3804, 3837, 3852, 3878, 3902, 3931, 3948, 3955, 3973, 3987, 4005, 4130, 4135, 4237, 4252, 4286, 4304, 4539, 4658, 4779, 4796, 4834, 4938, 5073, 5255, 5280, 5293, 5300, 5330, 5346, 5367, 5430, 5447, 5554, 5575, 5587, 5611, 5623], 'from': [25, 47, 216, 238, 603, 1245, 1296, 1571, 2386, 3024, 3128, 3139, 3144, 3166, 3176, 3181, 3211, 3348, 3516, 3542, 3791, 4287, 4321, 4958, 4985, 5010], 'bovine': [26, 39, 48, 188, 217, 230, 239, 379, 1247, 1269, 1291, 1297, 1509, 1558, 1565, 1581, 2084, 2494, 3026, 3129, 3213, 3246, 3545, 3707, 3803, 3857, 3901, 3912, 3947, 3972, 4288, 5054, 5283], 'small': [27, 49, 218, 240, 1248, 1298, 1620, 2085, 2233, 3130, 3247, 4289, 5305], 'intestine.': [28, 50, 219, 241, 1249], 'Based': [29, 220], 'on': [30, 66, 221, 257, 769, 1376, 1406, 1451, 1761, 2378, 3904, 4268], 'partial': [32, 223, 5055], 'amino': [33, 75, 106, 116, 224, 266, 297, 307, 1256, 1335, 2030, 3226, 3260, 3292, 3524, 3906, 3920, 3968, 4205, 4627], 'acid': [34, 76, 107, 225, 267, 298, 1257, 1336, 2031, 3227, 3261, 3293, 3593, 3813, 3907, 3969, 4206, 4628], 'sequence': [35, 62, 77, 226, 253, 268, 1780, 2036, 2041, 2044, 2047, 2140, 2381, 2552, 2603, 2629, 2647, 3228, 3294, 3474, 3556, 3825, 3970, 4031, 4811, 5033, 5083], 'enzyme,': [41, 139, 232, 330], 'its': [42, 233, 1187, 1284, 3010, 5344, 5563, 5591], 'cDNA': [43, 234, 429, 1265, 1582, 2062, 2087, 2597, 3298, 3322, 3331, 3426, 3892, 3924, 4972, 4984, 5060, 5082, 5624], 'has': [44, 235, 578, 1203, 4401, 4607, 4760, 5478], 'been': [45, 236, 802, 811, 1184, 1205, 4403, 4609, 5479], 'cloned': [46, 237, 812, 2353, 2439, 2527, 2679, 3287], 'The': [51, 177, 242, 368, 609, 1334, 1404, 1423, 1448, 1603, 1711, 1787, 2061, 2090, 2149, 2253, 2335, 2372, 2403, 2466, 2488, 2669, 2739, 2800, 2870, 2982, 3001, 3052, 3070, 3290, 3336, 3424, 3446, 3513, 3666, 3681, 3860, 3914, 3995, 4133, 4313, 4325, 4974, 5287, 5304, 5333, 5361, 5383], 'open': [52, 243, 1566, 2490, 3406, 3416, 3435, 3518, 3550, 3668, 3897, 3915], 'reading': [53, 244, 1567, 2491, 3407, 3417, 3436, 3519, 3551, 3669, 3898, 3916, 5764], 'frame': [54, 245, 2492, 3418, 3466, 3520, 3552, 3670, 3899, 3917], '1,605': [56, 247], 'base': [57, 248, 421], 'pairs': [58, 249], 'long,': [59, 250], 'this': [61, 252, 1251, 3297, 3314, 3517, 3549, 4017, 4030, 4035, 4219, 4245, 4782, 5091, 5261, 5766], 'produced': [63, 254, 3885, 5327, 5415], 'activity': [65, 184, 256, 375, 574, 677, 1281, 3003, 3691, 3712, 3775, 3838, 4253, 4540, 5448, 5507, 5555], 'transient': [67, 258, 2793, 3959], 'expression': [68, 259, 764, 2484, 2538, 2749, 2794, 2898, 3960, 5277, 5301, 5364, 5633, 5659], 'COS-7': [70, 261, 2907, 3679, 3754, 3962], 'cells.': [71, 262, 3680, 3755], 'Although': [72, 263, 3780, 4039, 4611], 'deduced': [74, 265, 3291, 3515, 4332], 'does': [78, 269, 4131], 'not': [79, 270, 1183, 1204, 3306, 3367, 3664, 3783, 3834, 4225, 4249, 4261, 4608, 4813, 5094, 5618], 'any': [81, 147, 272, 338, 2347], 'significant': [82, 273], 'homology': [83, 274], 'with': [84, 182, 275, 373, 474, 582, 756, 1397, 1428, 1457, 1494, 1705, 1793, 2065, 2157, 2239, 2249, 2341, 2364, 2412, 2498, 2522, 2543, 2606, 2641, 2663, 2675, 2715, 2718, 2946, 3021, 3118, 3274, 3480, 3819, 3974, 4784, 4807, 5343, 5566, 5682], 'other': [85, 276, 660, 1781, 2634, 3140, 3301, 3464, 3727, 3977, 4041, 4395, 4406, 4785, 5001], 'knownN-acetylglucosaminyltransferases': [86, 277], '(GnTs),': [87, 278], 'hydrophobicity': [89, 280], 'profile': [90, 281, 3588], 'showed': [91, 282, 3352, 3589, 3684, 4797, 5289], 'a': [92, 119, 283, 310, 579, 620, 1288, 1384, 1495, 1653, 2058, 2095, 2205, 2208, 2244, 2423, 2473, 2596, 2642, 2716, 2719, 2843, 2918, 3044, 3124, 3252, 3433, 3497, 3604, 3611, 3685, 3891, 3989, 4392, 4761, 4824, 5006, 5053, 5264, 5311, 5328, 5337, 5358, 5419, 5442, 5613], 'typical': [93, 284, 3612], 'type': [94, 285, 3613, 3990], 'II': [95, 286, 3614, 3991], 'transmembrane': [96, 120, 287, 311, 3605, 3992, 4002, 4803, 4852], 'protein': [97, 288, 3011, 3616, 3993, 4838], 'structure,': [98, 289, 5562], 'which': [99, 290, 476, 2841, 3701, 3761, 3821, 4514, 4930, 5307, 5340, 5351, 5440], 'common': [101, 292, 4616, 5265], 'to': [102, 293, 458, 483, 1175, 1191, 1383, 1413, 1585, 1590, 1598, 1609, 1770, 2081, 2257, 2345, 2480, 2535, 2548, 2748, 2770, 2774, 2777, 2806, 2997, 3009, 3013, 3187, 3257, 3346, 3400, 3456, 3531, 3598, 3698, 3704, 3731, 3757, 3850, 3929, 4142, 4276, 4299, 4308, 4772, 4791, 4835, 4848, 4925, 4933, 4988, 4992, 5030, 5077, 5423, 5502, 5559, 5570, 5630, 5656, 5664, 5696, 5720, 5728, 5730, 5745], 'many': [103, 294, 4040, 4549, 5258], 'glycosyltransferases.': [104, 295], 'N-terminal': [105, 296, 2545, 3555, 3695], 'sequencing': [108, 299, 1258, 2185], 'revealed': [113, 304, 3562, 3979, 5063], 'that': [114, 162, 305, 353, 593, 628, 675, 3304, 3313, 3353, 3563, 3590, 3608, 3798, 4016, 4034, 4218, 4234, 4258, 4516, 4533, 4551, 4757, 4798, 5064, 5299, 5481, 5573, 5607, 5700], '92': [115, 306], 'acids,': [117, 308], 'including': [118, 309, 3986], 'region,': [121, 312], 'were': [122, 313, 1344, 1446, 1468, 1583, 1607, 1666, 1701, 1759, 1768, 1790, 2195, 2255, 2338, 2351, 2392, 2444, 2589, 2658, 2687, 2742, 2762, 2795, 2959, 2984, 3142, 3185, 3194, 3205, 3220, 3305, 3623, 5027, 5066], 'truncated': [123, 314, 2038, 3569, 3715, 4201, 4310], 'during': [124, 315, 3574, 4399], 'purification.': [125, 316], 'Of': [126, 317], 'three': [128, 319, 2263, 4175, 4965, 5069], 'potential': [129, 320, 2779, 4176], 'N-glycosylation': [130, 321, 2780, 3619, 3827, 4177, 4197], 'sites': [131, 322, 2454, 2781, 3620], 'Asn-458': [132, 323, 4193, 4212], 'was': [133, 324, 1294, 1374, 1393, 1409, 1425, 1454, 1473, 1522, 1562, 1623, 1716, 2079, 2092, 2119, 2132, 2155, 2201, 2237, 2247, 2282, 2362, 2375, 2410, 2470, 2496, 2604, 2639, 2673, 2704, 2803, 2873, 2900, 3007, 3019, 3041, 3055, 3074, 3116, 3132, 3286, 3419, 3469, 3506, 3568, 3581, 3663, 3675, 3927, 4037, 4140, 4232, 4248, 4323, 4378, 4541, 4812, 4956, 4968, 5308, 5341, 5579], 'actually': [134, 325], 'glycosylated': [135, 326], 'although': [140, 331, 4244, 4842], 'thisN-glycosylation': [141, 332], 'site': [142, 333, 2504, 2515, 2612, 2726, 4198, 4606], 'could': [143, 334, 3324, 3366, 3602, 3717, 4019, 4207, 5256, 5387, 5568, 5603], 'be': [144, 335, 679, 1599, 1610, 3325, 3457, 3532, 3603, 3718, 3806, 4020, 4208, 4222, 4309, 4553, 4823, 5263, 5604, 5654, 5694, 5717], 'abolished': [145, 336, 3737], 'without': [146, 337], 'reduction': [148, 339], 'activity.': [151, 342, 3000, 3739], 'Serial': [152, 343, 2701], 'deletions': [153, 344, 2546, 3729, 3786], 'at': [154, 345, 2284, 2733, 2782, 2929, 2954, 2988, 3450, 3570, 3625, 3747, 3828, 4210, 4242, 4311, 5067, 5436, 5598], 'both': [155, 346, 2655, 5028], 'N': [157, 348, 1504, 3231, 3600], 'C': [159, 350, 3749, 4831], 'termini': [160, 351], 'proved': [161, 352], 'catalytic': [164, 355, 1277, 3800], 'domain': [165, 356, 1278, 3801, 4003, 4853], 'located': [169, 360, 3807, 4267, 4826], 'central': [172, 363], 'region': [173, 364, 2731, 3362, 3381, 3511, 3596, 3996, 4018, 4036, 4049, 4535, 4547, 5092], 'enzyme.': [176, 367, 3913], 'mRNA': [179, 370, 1622, 1656, 3053, 3115, 3853, 5294, 5314, 5331], 'level': [180, 371, 1286, 3054, 5278, 5355, 5634], 'correlated': [181, 372], 'enzymatic': [183, 374, 5690], 'various': [187, 378, 2483, 3025, 3066, 3856, 5282, 5398], 'tissues': [189, 380, 3027, 3067, 3141, 3858, 5284, 5498], 'tested.': [190, 381], '1The': [385], 'abbreviations': [386], 'used': [387, 1579, 1702, 1784, 1805, 2080, 2124, 2196, 2471, 2791, 2996, 3042, 3326, 5309], 'are:': [388], 'GnT-IV,': [389, 1197, 1510, 3708, 4929], 'β1,4-N-acetylglucosaminyl': [391], 'transferase;': [392], 'GnT,N-acetylglucosaminyltransferase;': [393], 'GlcNAc,N-acetyl-d-glucosamine;': [394], 'Man,': [395], 'mannose;': [396], 'GnT-V,': [397, 475, 5445], 'UDP-N-acetylglucosamine:α1,6-d-mannoside': [398, 407], 'β1,6-N-acetylglucosaminyltransferase': [399], 'V;': [400], 'EPO,': [401], 'erythropoietin;': [402], 'GnT-I,': [403, 796, 4646], 'β1,2-N-acetylglucosaminyltransferase;': [405, 408], 'GnT-II,': [406, 4650], 'GnT-III,': [409, 4654, 5406], 'UDP-N-acetylglucosamine:β-d-mannoside': [410], 'β1,4-N-acetylglucosaminyltransferase;': [411], 'PCR,': [412], 'polymerase': [413, 1795, 2159, 2163, 2261], 'chain': [414, 4247, 5686], 'reaction;': [415], 'RT-PCR,': [416], 'reverse': [417, 2396], 'transcription': [418, 1285, 2397], 'PCR;': [419], 'bp,': [420], 'pair(s);': [422], 'Gn2(2′,2)core-PA,': [423], 'GlcNAcβ1–2Manα1–6(GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc-pyridylamine;': [424], 'RACE,': [425], 'rapid': [426], 'amplification': [427], 'ends.': [430], '(EC': [431], '2.4.1.145)': [432], 'synthesis': [441], 'multiantennary': [444], 'structure': [445, 465, 493, 596, 4775, 5385, 5724], 'It': [450, 4255, 5477], 'specifically': [451], 'generates': [452], 'β1–4': [454, 767, 5376, 5410, 5595, 5645], 'linkage': [455, 480], 'GlcNAc': [457, 482, 768, 5377, 5414, 5435, 5596], 'α1–3': [460, 771, 5600], 'Man': [461, 486, 772], 'core': [464, 489], 'ofN-glycans': [466, 5386], '(Man3GlcNAc2).': [467], 'produces': [469, 477], 'structures': [471, 4599], 'cooperation': [473], 'β1–6': [479, 5434], 'α1–6': [485, 5438], 'structure.': [490, 3994], 'This': [491, 2360, 4389, 4546, 5602], 'dominant': [495], 'certain': [497, 682, 5380, 5493], 'glycoproteins,': [498], 'such': [499, 4843, 5404], 'as': [500, 1300, 2267, 2472, 2594, 2875, 3123, 3134, 3207, 3240, 3251, 3263, 4942, 4944, 5101, 5310, 5317, 5319, 5405], 'erythropoietin': [501, 2034], '(EPO)': [502], 'α1-acid': [504, 616], 'glycoprotein,': [505], 'very': [507, 5718, 5743], 'important': [508], 'for': [509, 1580, 1668, 1703, 1714, 1719, 1724, 1734, 1739, 1744, 1751, 1777, 1806, 1818, 2121, 2125, 2145, 2176, 2197, 2287, 2297, 2302, 2307, 2315, 2320, 2325, 2332, 2395, 2401, 2475, 2645, 2689, 2758, 2792, 2992, 3077, 3082, 3092, 3097, 3102, 3109, 3327, 3877, 3900, 4251, 4405, 4641, 4645, 4649, 4653, 4657, 4679, 4815, 5315, 5325, 5582, 5679, 5751, 5762], 'their': [510, 805, 4626, 4983], 'bioactivity': [511], '(1Takeuchi': [512], 'M.': [513, 519, 521, 737, 830, 861, 889, 979, 1002, 1069, 1083, 1103, 1223, 1317, 1540, 2574, 2824, 2883, 3484, 3642, 3646, 4051, 4084, 4156, 4361, 4409, 4458, 4479, 4661, 4671, 4686, 5115, 5143, 5189, 5460, 5509, 5539], 'Inoue': [514], 'N.': [515, 552, 780, 1006, 1028, 1030, 1042, 1052, 1101, 1131, 1133, 1159, 1219, 1313, 1536, 2820, 2858, 4088, 4152, 4357, 4462, 4666, 4742, 5208, 5218, 5243, 5456], 'Strickland': [516], 'T.W.': [517], 'Kubota': [518], 'Wada': [520, 1126], 'Shimizu': [522, 2575], 'R.': [523, 722, 840, 867, 906, 1048, 1091, 1093, 2558, 2570, 4061, 4419, 5121, 5160, 5214], 'Hoshi': [524], 'S.': [525, 529, 537, 774, 776, 848, 883, 1213, 1307, 1530, 2222, 2814, 2856, 2881, 4069, 4120, 4146, 4351, 4427, 4559, 4738, 4910, 5137, 5450], 'Kozutsumi': [526], 'H.': [527, 814, 842, 869, 893, 929, 951, 985, 1026, 1117, 1125, 1147, 1221, 1315, 1538, 2822, 3644, 4063, 4110, 4154, 4359, 4421, 4440, 4668, 4704, 4706, 4722, 5123, 5147, 5195, 5231, 5458], 'Takasaki': [528], 'Kobata': [530, 781, 5544], 'A.': [531, 538, 645, 687, 782, 849, 945, 998, 1022, 1050, 1119, 1151, 1350, 2223, 2564, 2852, 3640, 4070, 4080, 4121, 4428, 4454, 4736, 5216, 5235, 5545], 'Proc.': [532, 843, 2217, 4064, 4115, 4422], 'Natl.': [533, 844, 2218, 4065, 4116, 4423], 'Acad.': [534, 845, 2219, 4066, 4117, 4424], 'Sci.': [535, 846, 2220, 4067, 4118, 4425], 'U.': [536, 847, 2221, 4068, 4119, 4426], '1989;': [539, 711, 821], '86:': [540], '7819-7822Crossref': [541], 'PubMed': [542, 563, 653, 693, 714, 729, 789, 824, 853, 877, 900, 921, 939, 965, 992, 1016, 1036, 1059, 1079, 1113, 1141, 1166, 1235, 1329, 1355, 1552, 2227, 2584, 2836, 2865, 2889, 3491, 3658, 4074, 4098, 4125, 4168, 4373, 4432, 4450, 4472, 4489, 4511, 4571, 4585, 4696, 4716, 4732, 4748, 4872, 4887, 4916, 5046, 5131, 5154, 5175, 5202, 5225, 5250, 5472, 5521, 5535], 'Scopus': [543, 564, 654, 694, 715, 730, 790, 825, 854, 878, 901, 922, 966, 993, 1037, 1060, 1142, 1167, 1236, 1330, 1356, 1553, 2228, 2585, 2837, 2866, 2890, 3492, 3659, 4075, 4126, 4169, 4374, 4433, 4586, 4749, 4888, 4917, 5047, 5132, 5155, 5176, 5203, 5226, 5251, 5473], '(279)': [544], 'Google': [545, 566, 656, 696, 717, 732, 792, 827, 856, 880, 903, 924, 940, 968, 995, 1017, 1039, 1062, 1080, 1114, 1144, 1169, 1238, 1332, 1358, 1555, 2230, 2587, 2839, 2868, 2892, 3494, 3661, 4077, 4099, 4128, 4171, 4376, 4435, 4451, 4473, 4490, 4512, 4572, 4588, 4697, 4717, 4733, 4751, 4873, 4890, 4907, 4919, 5049, 5134, 5157, 5178, 5205, 5228, 5253, 5475, 5522, 5536, 5551], 'Scholar,': [546, 697, 718, 733, 828, 857, 881, 904, 925, 941, 969, 996, 1018, 1040, 1063, 1081, 1115, 1145, 4078, 4100, 4436, 4452, 4491, 4573, 4698, 4718, 4734, 4874, 4891, 4908, 5135, 5158, 5179, 5206, 5229, 5523, 5537], '2Boris': [547], 'P.N.': [548], 'Feger': [549], 'J.': [550, 556, 649, 705, 709, 908, 930, 954, 971, 987, 1007, 1031, 1054, 1070, 1089, 1104, 1121, 1155, 1161, 1224, 1318, 1541, 1631, 2566, 2825, 2854, 2885, 3647, 4089, 4157, 4362, 4441, 4463, 4480, 4493, 4502, 4562, 4580, 4687, 4707, 4723, 4863, 4901, 4912, 5162, 5181, 5197, 5220, 5239, 5245, 5461, 5511, 5512, 5526], 'Benbernou': [551], 'Rouzeau': [553], 'J.D.': [554, 895, 5149], 'Agneray': [555], 'Durand': [557], 'G.': [558, 707], 'Inflammation.': [559], '1990;': [560, 786, 2862, 4565], '14:': [561, 4914], '315-323Crossref': [562], '(71)': [565], 'Scholar).': [567, 657, 751, 793, 1170, 1333, 1359, 1556, 2231, 2869, 2893, 3495, 4172, 4589, 4752, 4920, 5050, 5254, 5476, 5552], 'For': [568], 'instance,': [569], 'vivo': [572, 4525], 'biological': [573, 5736], 'recombinant': [576, 4940, 5709, 5714], 'EPO': [577, 602, 2550, 2601, 2620, 3722, 4809], 'positive': [580], 'correlation': [581], 'ratio': [584], 'tetra-/biantennary': [586], 'Pharmacokinetics': [589], 'studies': [590], 'shown': [592, 1691, 3264], 'bulky': [595], 'branching': [600, 5384], 'prevents': [601], 'filtering': [604], 'out': [605, 2134, 2203, 2706], 'into': [606, 1799, 2166, 2354, 2528, 2680, 2744, 2764, 2902], 'urine.': [608], 'increase': [610, 3687, 5483, 5564], 'antenna': [613, 5486], 'number': [614, 5487], 'glycoprotein': [617], 'observed': [618, 4404], 'under': [619], 'chronic': [621], 'inflammatory': [622], 'state': [623, 4280], 'or': [624, 2161, 2173, 2737, 3637, 5590], 'liver': [625, 3884], 'damage': [626], 'suggested': [627, 674, 4033, 5480], 'some': [629, 3197], 'clinical': [630], 'situations': [631], 'and/or': [632, 807, 5671], 'cellular': [633, 5381, 5571], 'disorders': [634], 'may': [635, 5368], 'also': [636, 761, 1274, 3223, 3741, 4032, 4402, 5335, 5557, 5755], 'affect': [637], 'branch': [639, 1201, 5378, 5402, 5597, 5650, 5703, 5723], 'formation': [640, 1202, 5651, 5704], 'chains': [643, 5491], '(3Mackiewicz': [644], 'Mackiewicz': [646], 'K.': [647, 778, 887, 1004, 2554, 2879, 4086, 4460, 4497, 4561, 4577, 5141, 5541, 5543], 'Glycoconj.': [648, 2884], '1995;': [650, 726, 957, 989, 1163, 4884, 4904, 4913, 5199, 5247], '12:': [651, 898, 5152], '241-247Crossref': [652], '(114)': [655, 1357], 'On': [658, 2632, 3725, 4999], 'hand,': [661, 2635, 3728], 'structural': [662], 'analyses': [663], 'oligosaccharides': [665], 'hepatoma': [667], 'cell': [668, 3016, 5494], 'lines': [669, 5495], 'choriocarcinoma': [672], 'should': [678], 'high': [680, 1478, 5345], 'kinds': [683], 'tumors': [685], '(4Kobata': [686], 'Biochimie': [688], '(Paris).': [689], '1988;': [690, 2224, 4710, 4745], '70:': [691], '1575-1585Crossref': [692], '(33)': [695], '5Campion': [698], 'B.': [699, 927, 953, 975, 1087, 4438, 4720, 5185], 'Leger': [700], 'D.': [701, 1095], 'Wieruszeski': [702], 'J.-M.': [703], 'Montreuil': [704], 'Spik': [706], 'Eur.': [708, 986, 1053, 1160, 4900, 5196, 5219, 5244], 'Biochem.': [710, 784, 870, 988, 1032, 1055, 1134, 1162, 4581, 4744, 5124, 5198, 5221, 5246], '184:': [712], '405-413Crossref': [713], '(64)': [716], '6Goulut-Chassaing': [719], 'C.': [720, 1129, 2556], 'Bourrillon': [721], 'Biochim.': [723, 2859], 'Biophys.': [724, 785, 871, 1135, 2860, 5125], 'Acta.': [725, 2861], '1244:': [727], '30-40Crossref': [728], '(9)': [731], '7Dennis': [734], 'J.W.': [735], 'Fukuda': [736], 'Cell': [738, 742, 4902], 'Surface': [739], 'Carbohydrates': [740], 'Development.': [743], 'CRC': [744], 'Press,': [745], 'Inc.,': [746], 'Boca': [747], 'Raton,': [748], 'FL1991:': [749], '161-194Google': [750], 'Rat': [752], '3Y1': [753], 'cells': [754, 2908, 2958, 3683, 3963, 4947], 'transformed': [755], 'adenovirus': [758], '12': [759], 'gene': [760, 1188], 'elevated': [762], '(8Hiraizumi': [773], 'Taksaki': [775], 'Shiroki': [777], 'Kochibe': [779], 'Arch.': [783], '280:': [787], '9-19Crossref': [788], '(13)': [791], 'To': [794, 1501], 'date,': [795], '-II,': [797], '-III,': [798], '-V': [800, 5107], 'cDNAs': [806, 5639], 'genomic': [808, 5056, 5074, 5110, 5268], 'DNAs': [809, 5269], '(9Schachter': [813], 'Brockhausen': [815], 'I.': [816], 'Hull': [817, 831, 890, 4052, 4410, 5144], 'E.': [818, 832, 859, 891, 1044, 4053, 4411, 5113, 5145, 5210], 'Methods': [819], 'Enzymol.': [820], '179:': [822], '351-397Crossref': [823], '(49)': [826], '10Sarkar': [829], 'Nishikawa': [833, 1021, 1049, 1118, 1150, 2849, 4054, 4412, 5215, 5234], 'Y.': [834, 1000, 1020, 1046, 1123, 1127, 1153, 1157, 1217, 1311, 1534, 2818, 4055, 4082, 4150, 4355, 4413, 4456, 4575, 4664, 4700, 4858, 5212, 5237, 5241, 5454], 'Simpson': [835, 948, 4056, 4414], 'R.J.': [836, 949, 2562, 4057, 4415], 'Moritz': [837, 946, 4058, 4416], 'R.L.': [838, 910, 947, 4059, 4417, 5164], 'Dunn': [839, 866, 4060, 4418, 5120], 'Schachter': [841, 868, 892, 928, 950, 984, 4062, 4420, 4439, 4705, 4721, 5122, 5146, 5194], '1991;': [850, 874, 4071, 4429, 4582, 5128], '88:': [851, 4072, 4430], '234-238Crossref': [852, 4073, 4431], '(103)': [855, 1038, 4076, 4434], '11Hull': [858], 'Sarkar': [860, 888, 978, 5114, 5142, 5188], 'Spruijt': [862, 5116], 'M.P.N.': [863, 5117], 'Hoppener': [864, 5118], 'J.W.M.': [865, 5119], 'Res.': [872, 1136, 3487, 5042, 5126, 5547], 'Commun.': [873, 1137, 5127], '176:': [875, 5129], '608-615Crossref': [876, 5130], '(54)': [879, 5133], '12Pownall': [882, 5136], 'Kozak': [884, 5138], 'C.A.': [885, 5139], 'Schappert': [886, 5140], 'Marth': [894, 5148], 'Genomics.': [896, 5150], '1992;': [897, 918, 1010, 1073, 1352, 4092, 4466, 4483, 4690, 5151, 5172], '699-704Crossref': [899, 5153], '(63)': [902, 5156], '13Kumar': [905, 5159], 'Yang': [907, 5161], 'Eddy': [909, 5163], 'Byers': [911, 5165], 'M.G.': [912, 1065, 4475, 4682, 4880, 5166], 'Shows': [913, 5167], 'T.B.': [914, 5168], 'Stanley': [915, 5169], 'P.': [916, 983, 1099, 5170, 5193], 'Glycobiology.': [917, 5171], '2:': [919, 5173], '383-393Crossref': [920, 5174], '(57)': [923, 994, 5177, 5204], '14Bendiak': [926, 4437], 'Biol.': [931, 955, 1008, 1071, 1105, 1225, 1319, 1542, 2826, 3648, 4090, 4158, 4363, 4442, 4464, 4481, 4503, 4563, 4688, 4708, 4724, 4864, 4882, 4903, 5462, 5513, 5527], 'Chem.': [932, 956, 1009, 1072, 1106, 1226, 1320, 1543, 2827, 3649, 4091, 4159, 4364, 4443, 4465, 4482, 4504, 4564, 4689, 4709, 4725, 4865, 5463, 5514, 5528], '1987;': [933, 4444, 4505, 4726, 5529], '262:': [934, 4445, 4506, 4727, 5530], '5775-5783Abstract': [935, 4446], 'Full': [936, 960, 962, 1013, 1076, 1110, 1230, 1232, 1324, 1326, 1547, 1549, 2831, 2833, 3653, 3655, 4095, 4163, 4165, 4368, 4370, 4447, 4469, 4486, 4508, 4568, 4693, 4713, 4729, 4869, 5467, 5469, 5518, 5532], 'Text': [937, 961, 963, 1014, 1077, 1111, 1231, 1233, 1325, 1327, 1548, 1550, 2832, 2834, 3654, 3656, 4096, 4164, 4166, 4369, 4371, 4448, 4470, 4487, 4509, 4569, 4694, 4714, 4730, 4870, 5468, 5470, 5519, 5533], 'PDF': [938, 964, 1015, 1078, 1112, 1234, 1328, 1551, 2835, 3657, 4097, 4167, 4372, 4449, 4471, 4488, 4510, 4570, 4695, 4715, 4731, 4871, 5471, 5520, 5534], "15D'Agostaro": [942], 'G.A.F.': [943, 973, 5183], 'Zingoni': [944], 'Bendiak': [952, 974, 5184], '270:': [958], '15211-15221Abstract': [959], '(58)': [967], '16Tan': [970, 5180], "D'Agostaro": [972, 5182], 'Reck': [976, 5186], 'F.': [977, 5187], 'Squire': [980, 5190], 'J.A.': [981, 5191], 'Leong': [982, 5192], '231:': [990, 5200], '317-328Crossref': [991, 5201], '17Nishikawa': [997, 4079, 4453], 'Ihara': [999, 1045, 1122, 1152, 1216, 1310, 1533, 2817, 4081, 4149, 4354, 4455, 5211, 5236, 5453], 'Hatakeyama': [1001, 4083, 4457], 'Kangawa': [1003, 4085, 4459], 'Taniguchi': [1005, 1029, 1051, 1132, 1158, 1218, 1312, 1535, 2819, 2857, 4087, 4151, 4356, 4461, 4741, 5217, 5242, 5455, 5748], '267:': [1011, 1074, 4093, 4467, 4484, 4691], '18199-18204Abstract': [1012, 4094, 4468], '18Ihara': [1019], 'Tohma': [1023], 'T.': [1024, 1635, 2576, 2578, 4579, 4662, 4740], 'Soejima': [1025, 1124], 'Niikawa': [1027, 1130], '1993;': [1033, 1107], '113:': [1034], '692-698Crossref': [1035], '19Koyama': [1041, 5207], 'Miyoshi': [1043, 5209], 'Kang': [1047, 5213], '1996;': [1056, 2886, 5222], '238:': [1057, 5223], '853-861Crossref': [1058, 5224], '(19)': [1061, 5227], '20Shoreibah': [1064], 'Hindsgaul': [1066, 4476, 4683], 'O.': [1067, 4477, 4684], 'Pierce': [1068, 1102, 4478, 4685], '2920-2927Abstract': [1075, 4485, 4692], '21Shoreibah': [1082], 'Perng': [1084], 'G.-S.': [1085], 'Adler': [1086], 'Weinstein': [1088], 'Basu': [1090], 'Cupples': [1092], 'Wen': [1094], 'Browne': [1096], 'J.K.': [1097], 'Buckhaults': [1098], 'Fregien': [1100], '268:': [1108], '15381-15385Abstract': [1109], '22Saito': [1116], 'Gu': [1120, 1148, 2853, 5232], 'Sekiya': [1128], '1994;': [1138, 4866], '198:': [1139], '318-327Crossref': [1140], '(100)': [1143], '23Saito': [1146, 5230], 'J.G.': [1149, 5233], 'Fujii': [1154, 2855, 4737, 5238], 'Kohgo': [1156, 5240], '233:': [1164, 5248], '18-26Crossref': [1165, 5249], '(46)': [1168, 5252], 'Among': [1171], 'necessary': [1174], 'produce': [1176, 2536, 4792, 5560], 'chains,': [1179], 'only': [1180, 3459, 4196], 'had': [1182, 3762, 4136, 4187, 4238, 4548, 4980, 5005, 5088, 5352], 'isolated': [1185, 3890], 'nor': [1186, 3500], 'cloned.': [1189], 'Due': [1190], 'lack': [1193], 'data': [1195, 1574, 4595, 4783], 'concerning': [1196], 'mechanism': [1199], 'fully': [1206], 'clarified.': [1207], 'Recently': [1208], 'Oguri': [1209, 2810, 2880], 'et': [1210, 2811, 2850], 'al.': [1211, 2812], '(24Oguri': [1212, 1306, 1529, 2813, 4145, 5449], 'Minowa': [1214, 1308, 1531, 2815, 4147, 4352, 5451], 'M.T.': [1215, 1309, 1532, 2816, 4148, 4353, 5452], 'Ikenaga': [1220, 1314, 1537, 2821, 3643, 4153, 4358, 5457], 'Takeuchi': [1222, 1316, 1539, 2823, 2882, 3645, 4155, 4360, 5459], '1997;': [1227, 1321, 1544, 2828, 3650, 4160, 4365, 5464, 5548], '272:': [1228, 1322, 1545, 2829, 3651, 4161, 4366, 5465], '22721-22727Abstract': [1229, 1323, 1546, 2830, 4162, 4367, 5466], '(55)': [1237, 1331, 1554, 2838, 4170, 4375, 5474], 'Scholar)': [1239, 1651, 2588, 3662, 4377], 'characterized': [1243], 'In': [1250, 4024], 'report,': [1252], 'describe': [1254], 'enzyme': [1263, 1343, 1372, 1405, 1516, 3567, 3575, 3716, 4186, 4342, 4759, 5324, 5588], 'cloning': [1266, 5316], 'gene.': [1271], 'We': [1272, 3222, 3242, 3365, 3740, 3844, 3888, 4769, 5274, 5741, 5754], 'localized': [1275], 'examined': [1283], 'variety': [1289, 5614], 'tissues.': [1292, 3867, 4949], 'intestine': [1299, 1621, 2086, 2234, 3131, 3248], 'described': [1301, 3135], 'our': [1303, 3758], 'previous': [1304], 'report': [1305], 'sequences': [1337, 1817, 2032, 2391, 2691, 3193, 3204, 3338, 3540, 3908, 3944, 4045, 5015], 'protease-digested': [1339], 'fragments': [1340, 1442, 1676, 1789, 2741, 3063, 3174, 3209, 3239, 3303], 'determined': [1345, 3224], 'by': [1346, 1395, 1419, 1475, 1525, 1625, 1728, 1765, 2204, 2291, 2369, 2591, 2707, 2767, 2797, 2915, 2980, 2986, 3086, 3296, 3372, 3634, 3932, 3958, 4344, 5285, 5392, 5416], "Iwamatsu's": [1347], 'method': [1348, 1629, 2210, 2808, 2847, 5581], '(25Iwamatsu': [1349], 'Electrophoresis.': [1351], '13:': [1353, 2887], '142-147Crossref': [1354], 'Briefly,': [1360], 'after': [1361, 1518], 'second': [1363, 2127, 2373, 2407, 3374], 'affinity': [1364], 'column': [1365], 'chromatography': [1366, 1481], 'step,': [1367], '300': [1368, 3147], 'pmol': [1369, 1512, 1689, 3037, 3148], 'preparation': [1373, 3949], 'separated': [1375, 1474], '10%': [1377, 2947], 'SDS-polyacrylamide': [1378, 4345], 'gel': [1379, 1520, 1764, 4346], 'electrophoresis.': [1380], 'After': [1381, 2932, 3409], 'transfer': [1382], 'polyvinylidene': [1385], 'difluoride': [1386], 'membrane': [1387, 1408, 1453, 3615], '(ProBlott,': [1388], 'Perkin-Elmer),': [1389], 'band': [1392], 'identified': [1394, 3206], 'staining': [1396], 'Ponceau': [1398], 'S': [1399], '(Sigma)': [1400], 'cut': [1402], 'out.': [1403], 'reduced': [1410, 3056], 'S-carboxymethylated': [1412], 'allow': [1414], 'detection': [1415, 5584], 'Cys': [1417, 1880], 'residues': [1418, 4986], 'peptide': [1421, 1424, 1449, 1471, 1497, 1527, 1675, 1779, 3157, 3188, 3192, 3203, 3302, 3539, 3943, 4215, 4333], 'sequencer.': [1422], 'then': [1426, 1791, 2352], 'digested': [1427, 2521, 2674], 'lysil': [1430], 'endopeptidase': [1431, 1459], 'Achromobacter': [1432], 'protease': [1433, 3161, 4027, 4293], 'I': [1434, 1694, 3162], '(Wako': [1435], 'Pure': [1436, 1490], 'Chemicals,': [1437], 'Osaka,': [1438], 'Japan),': [1439], 'released': [1443], '(AP': [1444], 'fragments)': [1445, 1467], 'collected.': [1447, 1469], 'remaining': [1450], 'further': [1455, 3785], 'incubated': [1456], 'asparagil': [1458], 'Asp-N': [1460, 3169], '(Boehringer': [1461], 'Mannheim),': [1462], 'digests': [1465], '(DN': [1466], 'Each': [1470], 'fragment': [1472, 2063, 2104, 2151, 2417, 2425, 2434, 2598, 3272, 3316, 3427, 3893, 3925], 'reversed': [1476], 'phase': [1477], 'performance': [1479], 'liquid': [1480], '(Wakosil-II': [1482], 'AR': [1483], '5C18': [1484], '2.0': [1485], '×': [1486, 2904, 2990], '150': [1487, 2107], 'mm,': [1488], 'Wako': [1489], 'Chemicals)': [1491], 'analyzed': [1493, 1524, 1760, 5275], 'gas-phase': [1496], 'sequencer': [1498, 1528, 2191], '(PPSQ-10,': [1499], 'Shimadzu).': [1500], 'determine': [1502, 4934, 5701], 'terminus': [1505, 3232, 3601, 3750, 4832], '120': [1511], 'final': [1515, 2670], 'preparation,': [1517, 3153], 'filtration,': [1521], 'directly': [1523], 'A': [1557, 1638, 2103, 2129, 3270, 4962, 5412], 'codon': [1559, 2617, 2721, 3371, 3449, 3468], 'usage': [1560], 'table': [1561, 2056], 'constructed': [1563, 2590], 'using': [1564, 1773, 2094, 2135, 2259, 2709, 2917, 3047, 3245, 3378, 3720, 3847, 5707], 'frames': [1568], 'randomly': [1569], 'selected': [1570], 'GenBankTM': [1573], 'base.': [1575], 'Only': [1576], 'codons': [1577, 2766], 'frequently': [1578], 'adopted': [1584], 'design': [1586], 'primers': [1587, 1595, 1604, 1663, 1687, 2349, 2499, 2665, 3255], 'probes': [1589, 1597, 1606, 1665, 1774], 'minimize': [1591], 'variation': [1593, 5087], 'less': [1600], 'than': [1601, 1612, 1782, 4381], '10,000.': [1602], 'designed': [1608], 'greater': [1611], '29-': [1613], '41-mer': [1615], 'length,': [1617], 'respectively.': [1618, 2700, 2756, 2786], 'Bovine': [1619, 2232], 'prepared': [1624, 2874, 3235], 'guanidinium': [1627], 'thiocyanate/CsCl': [1628], '(26Sambrook': [1630], 'Fritsch': [1632, 2571], 'E.F.': [1633, 2572], 'Maniatis': [1634], 'Molecular': [1636], 'Cloning:': [1637], 'Laboratory': [1639], 'Manual.': [1640], '2nd': [1641], 'Ed.': [1642], 'Cold': [1643, 1647], 'Spring': [1644, 1648], 'Harbor': [1645], 'Laboratory,': [1646], 'Harbor,': [1649], 'NY1989Google': [1650], 'Poly(A)': [1654], 'Quik': [1655], 'isolation': [1657], 'kit': [1658, 1709], '(Stratagene).': [1659, 2252], 'Sense': [1660], 'antisense': [1662, 2073, 2269, 2625, 3282], 'synthesized': [1667], 'AP-5,': [1670], 'AP-12,': [1671], 'AP-15,': [1672], 'DN-9': [1674, 2072, 3281], '(Table': [1677, 1775, 3284], 'I).': [1678], 'One': [1679], 'possible': [1682, 4257, 4756, 5629, 5655], 'combinations': [1683], 'sense/antisense': [1686], '(50': [1688, 2271, 2274], 'each)': [1690], 'Table': [1693], '0.5': [1696], 'μg': [1697, 2895], 'poly(A)+': [1699, 2235, 3022, 3249, 3848], 'RNA': [1700, 2236, 3023, 3127, 3250, 3849], 'RT-PCR': [1704, 1708, 1715, 1757, 1788, 1807, 1819, 3018, 3050, 3244, 3310, 3846], 'an': [1706, 2189, 2502, 2513, 2624, 2723, 3048, 3591, 5353, 5689], 'Access': [1707, 3049], '(Promega).': [1710], 'temperature': [1712, 3071], 'cycle': [1713, 2184, 3072], '48': [1717, 3075], '°C': [1718, 1723, 1733, 1738, 1743, 1750, 2286, 2296, 2301, 2306, 2314, 2319, 2324, 2331, 3076, 3081, 3091, 3096, 3101, 3108], '45': [1720, 3078], 'min,': [1721, 1726, 1741, 2304, 3079, 3084, 3099], '94': [1722, 1732, 2285, 2295, 2313, 3080, 3090], '2': [1725, 1745, 2692, 2972, 3083, 3103, 3392], 'followed': [1727, 2290, 3085], '50': [1729, 2300, 2318], 'cycles': [1730, 2293, 2311, 3088, 3411], '30': [1735, 2298, 2316, 2321, 3087, 3093], 's,': [1736, 2299, 2317, 2322, 3094], '60': [1737], '1': [1740, 2303, 2308, 2326, 2630, 2648, 2974, 3098, 3422, 4996], '68': [1742, 1749, 3100, 3107], 'min;': [1746, 2309, 2327, 3104], 'finally': [1748, 2329, 3106], '7': [1752, 3110], 'min.': [1753, 2334, 3111], 'Aliquots': [1754, 2653], 'products': [1758, 2254, 2337, 2657], '1%': [1762], 'agarose': [1763], 'electrophoresis': [1766, 4347], 'subjected': [1769, 2256, 3186], 'Southern': [1771], 'analysis': [1772, 3557], 'I)': [1776, 3285], 'those': [1783, 3975], 'RT-PCR.': [1786, 5286], 're-amplified': [1792], 'Taq': [1794, 2162], '(Stratagene)': [1796, 2160, 2172], 'subcloned': [1798, 2165, 2743], 'pT7Blue': [1801, 2174, 2442], 'T-Vector': [1802, 2175, 2443], '(Novagen).Table': [1803], 'IOligonucleotides': [1804], 'construction': [1809], 'expressed': [1812, 3677, 3710, 3743], 'fusion': [1813], 'proteinsPrimer': [1814], 'probe': [1816, 2091], 'AP-5aNames': [1820], 'peptides': [1823, 2052], 'are': [1824, 2053, 4174, 4845, 4923, 5099, 5742], 'underlined.Amino': [1825], 'acidLys': [1826, 1874], 'Ile': [1827, 1928, 1929], 'His': [1828, 1987], 'Val': [1829, 1835, 1979, 1981], 'Asn': [1830, 1876, 1980], 'Pro': [1831, 1832, 1931, 1932, 1937, 1978], 'Ala': [1833, 1838, 1886, 2765], 'Glu': [1834, 1927, 1933, 1982], 'Ser': [1836, 1930, 1934, 1983, 1988], 'Thr': [1837, 1875], 'LeuPrimer': [1839], '(S)5′-AAR': [1840], 'ATY': [1841, 1860, 1941, 1942, 1963, 1964], 'CAY': [1842], 'GTB': [1843, 1994, 1996], 'AAY': [1844, 1995], 'CCH': [1845, 1846, 1864, 1865, 1944, 1945, 1966, 1967, 1972, 1993, 2016], 'GCN': [1847, 1897], 'GAR': [1848, 1946, 1997], 'GT-3′Primer': [1849], '(AS)3′-TTY': [1850], 'TAR': [1851, 1952, 1953], 'GTR': [1852], 'CAV': [1853, 2006, 2008], 'TTR': [1854, 2007], 'GGD': [1855, 1856, 1955, 1956, 2005], 'CGN': [1857, 1908], 'CTY': [1858, 1957, 2009], 'CA-5′Probe5′-AAG': [1859], 'CAC': [1861, 2025], 'GTS': [1862, 1868, 2017, 2019], 'AAC': [1863, 1912, 2018], 'GCH': [1866, 1871, 1922], 'GAG': [1867, 1962, 1968, 2020], 'WSY': [1869, 1943, 1947, 1965, 1969, 1998, 2021, 2026], 'ACM': [1870, 1911], 'CT-3′': [1872, 1974], 'AP-12Amino': [1873], 'Leu': [1877, 1882, 1985], 'Asp': [1878, 1938], 'Tyr': [1879, 1885, 1935, 1936, 1984], 'Phe': [1881, 1986], 'Met': [1883, 1884], 'Gln': [1887], 'GluPrimer': [1888], '(S)5′-GAY': [1889, 1991], 'TAY': [1890, 1896, 1948, 1949, 1999], 'TGY': [1891], 'TTY': [1892, 1917, 2001, 2004, 2024], 'YTB': [1893], 'ATG': [1894, 1895, 1919, 1920, 2508, 2616], 'CAG': [1898, 1923], 'GA-3′Primer': [1899], '(AS)5′-CTR': [1900], 'ATR': [1901, 1907, 1959, 1960, 2011], 'ACR': [1902], 'AAR': [1903, 1992, 2013], 'GAV': [1904, 2012], 'TAC': [1905, 1906, 1915, 1921, 1970, 1971, 2022], 'GTC': [1909], 'CT-5′Probe5′-AAG': [1910], 'CTS': [1913, 1918, 2023], 'GAC': [1914, 1973], 'TGC': [1916], 'GAG-3′': [1924], 'AP-15Amino': [1925], 'acid-Val': [1926], 'Leu—Primer': [1939], '(S)5′-GAR': [1940], 'CC-3′Primer': [1950], '(AS)3′-CTY': [1951], 'WSR': [1954, 1958, 2010], 'GG-5′Probe5′-GTS': [1961], 'DN-9Amino': [1975], 'acidAsp': [1976], 'Lys': [1977], 'Gly': [1989], 'AsnPrimer': [1990], 'TTB': [2000], 'CA-3′Primer': [2002], '(AS)3′-CTR': [2003], 'GT-5′Probe5′-GAC': [2014], 'AAG': [2015], 'GGV': [2027], 'AAC-3′Three': [2028], 'junction': [2029, 2628, 2646, 2690], 'signal': [2035, 2551, 2602, 2621, 3505, 3723, 4810, 4825], 'Junction': [2040, 2043, 2046], '1-LeuProValLeuGlyAlaProIleLeuLysGluLeuThrSerLysLys-↲': [2042], '2-LeuProValLeuGlyAlaProHisLeuLeuGlnAsnGluGlySer-↲': [2045], '3-LeuProValLeuGlyAlaProTyrValLysArgGluValLys-↲a': [2048], 'Names': [2049], 'underlined.': [2054], 'Open': [2055], 'new': [2059], 'tab': [2060], 'amplified': [2064, 2120, 2156, 2497, 2605, 2640, 2661, 2740, 3062, 3117, 3273, 3315, 3863], 'AP-5': [2067, 3276], 'sense': [2068, 2643, 3277], 'primer': [2069, 2074, 2241, 2270, 2609, 2626, 2644, 2712, 2717, 3040, 3278, 3283], '(Fig.': [2075, 2141, 2367, 2486, 2789, 3343, 3363, 3389, 3429, 3452, 3526, 3553, 3692, 3778, 3839, 3950, 4011, 4818], '1,': [2076, 2142, 3344, 3390], 'Probe': [2077, 2117, 2143, 2365, 2413, 3334, 3391], '1)': [2078, 2368, 3935], 'screen': [2082], 'library': [2088, 2361, 2404, 3332], '(CLONTECH).': [2089], '32P-labeled': [2093], 'PCR': [2096, 2258, 2281, 2336, 2478, 2593, 2656, 2671, 2708, 2768], 'radioactive': [2097], 'labeling': [2098, 2122], 'system': [2099], '(Life': [2100, 2942, 2951], 'Technologies,': [2101, 2943, 2952], 'Inc.).': [2102], 'approximately': [2106], 'bp': [2108, 3439, 3443, 5079], '5′-end': [2111], 'phage': [2113, 2137, 2154, 2427, 2437, 3387], 'clone': [2114, 2138, 2384, 2421, 2428, 2458, 5003, 5057], '#1e': [2115, 3384], '(Fig.1,': [2116, 3333], '2)': [2118, 3430, 3453, 3527, 3952], 'screening.': [2128, 2148], 'similar': [2130, 5029], 'procedure': [2131, 4298, 4394], 'carried': [2133, 2202, 2705], '#2a': [2139, 3386], '3)': [2144, 4012], 'third': [2147, 3376], 'insert': [2150, 2438], 'each': [2153, 2897, 3005, 3039, 4758], 'Pfu': [2158, 2260], 'pCR-Script': [2168, 2356, 2462], 'Amp': [2169, 2357, 2463], 'SK(+)': [2170, 2358, 2464], 'vector': [2171, 2531, 2539, 3674], 'nucleotide': [2177, 3337, 3473], 'sequencing.': [2178, 2199, 2799], 'Dye': [2179, 2182], 'Primer': [2180, 2390], 'Terminator': [2183], 'kits': [2186], '(Perkin-Elmer)': [2187, 2194], 'ABI': [2190], 'model': [2192], '377': [2193], 'DNA': [2198, 2380, 2798, 5014, 5075], '5′-RACE': [2200, 2374, 2408, 2420, 2457, 3399, 4960, 4966, 4975], 'modification': [2206, 2844], 'published': [2209], '(27Frohman': [2211], 'M.A.': [2212], 'Dush': [2213], 'M.K.': [2214], 'Martin': [2215], 'G.R.': [2216], '85:': [2225], '8998-9002Crossref': [2226], '(4336)': [2229], 'reverse-transcribed': [2238], 'RRT1': [2240], '(5′-TATTGAAACTCCTGTTCGTCCATTTCCGAT-3′),': [2242], 'poly(dA)': [2245], 'tail': [2246, 3499], 'added': [2248, 4292], 'terminal': [2250], 'deoxynucleotidyltransferase': [2251], 'primers:': [2264], 'RPCR1': [2265], '(5′-CTGCACGGCAGGTTGAAGGCTTCCT-3′)': [2266], 'pmol);': [2272], 'adaptor': [2273], 'pmol),': [2275], '5′-GCCGCATGCGAATTCACC-3′;': [2276], 'adaptor-(dT)17': [2278], '(6': [2279], 'pmol).': [2280], 'set': [2283], '5': [2288, 2333, 2903, 2993], 'min': [2289, 2994], '20': [2292, 2310, 3941], '72': [2305, 2323, 2330, 2933], 'twice': [2340], 'Centricon': [2342], '100': [2343, 2963], '(Amicon)': [2344], 'remove': [2346], 'excess': [2348], 'vector.': [2359, 2465], 'screened': [2363, 2411], '3': [2366], 'colony': [2370], 'hybridization.': [2371], 'performed': [2376, 2804, 3020, 3243, 3398, 3845], 'based': [2377, 3903], 'resulting': [2385, 2682, 2695], 'first': [2388, 3350, 3447], '5′-RACE.': [2389], 'RRT2': [2393], '(5′-AATTGCTGTACAATGGCACTGAGCTCAGA-3′)': [2394], 'RPCR2': [2399], '(5′-AGAGCGCTGAGAGATTCGA-3′)': [2400], 'PCR.': [2402], 'clones': [2409, 3342, 4967, 4976, 5023], '4': [2414], '(Fig.1).': [2415], 'AnEco47III-HpaI': [2416], '#3a,': [2422], 'HpaI-BstXI': [2424], '#2a,': [2429], 'aBstXI-KpnI': [2431], '(in': [2432, 2452], 'vector)': [2433, 2453], '#1a': [2436], 'sequentially': [2445], 'ligated': [2446, 2547], 'between': [2447, 3808, 3855, 3866, 3997, 4330, 4542, 4827, 5052], 'Eco47III': [2449], 'KpnI': [2451], '#4a': [2459, 5004], 'resultant': [2467], 'plasmid,': [2468], 'pCRBGT4,': [2469], 'template': [2474, 3253], 'following': [2477, 2727, 3168], 'experiments': [2479, 4531], 'make': [2481, 3784], 'vectors': [2485, 2542], '1).': [2487, 3335, 3364], 'entire': [2489, 3667, 3896], '4EXPF2': [2500, 2711], '(introducing': [2501, 2512, 2610], 'XhoI': [2503, 2523, 2676], 'upstream': [2505, 2613, 3440], 'codon)': [2509], '4EXPR': [2511, 2651], 'XbaI': [2514, 2724], 'downstream': [2516, 3444], 'stop': [2519], 'codon),': [2520], 'XbaI,': [2525], 'pSVL': [2530, 3673], '(Amersham': [2532], 'Pharmacia': [2533], 'Biotech)': [2534], 'pBGT4.': [2540], 'Expression': [2541], 'serial': [2544, 3744], '(28Jacobs': [2553], 'Shoemaker': [2555], 'Rubersdorf': [2557], 'Neill': [2559], 'S.D.': [2560], 'Kaufman': [2561], 'Mufson': [2563], 'Seehra': [2565], 'Jones': [2567], 'S.S.': [2568], 'Hewick': [2569], 'Kawakita': [2573], 'Miyake': [2577], 'Nature': [2579], '(London).': [2580], '1985;': [2581], '313:': [2582], '806-810Crossref': [2583], '(850)': [2586], 'overlapping': [2592], 'follows:': [2595], 'encoding': [2599, 2627, 5270], 'XhoEsig': [2608, 2666], 'anXhoI': [2611], 'sequence)': [2622], '(TableI).': [2631], 'segment': [2638, 5008], 'primer.': [2652], 'combined': [2659], 'again': [2662], '4EXPR.': [2668], 'product': [2672, 5443, 5576, 5592], 'andXbaI': [2677], 'pSVL,': [2681, 2745], 'pSigIle93.': [2684], 'Similar': [2685], 'procedures': [2686], 'employed': [2688, 3073, 3307], '3,': [2694, 3394], 'pSigPro113': [2697], 'pSigPro142,': [2699], 'C-terminal': [2702, 3769, 3789, 4529], 'deletion': [2703, 3696, 3745, 3765, 3770, 3794, 4530], 'combination': [2714], 'TGA': [2720], 'restriction': [2725], 'coding': [2730, 3361], 'terminating': [2732], 'Gly-499,': [2734], 'Pro-465,': [2735], 'Lys-432,': [2736], 'Pro-383.': [2738, 3811, 4545], 'giving': [2746], 'rise': [2747], 'plasmids': [2750], 'pCGly499,': [2751], 'pCPro465,': [2752], 'pCLys432,': [2753], 'pCPro383,': [2755, 3760], 'Codons': [2757], 'Ser-79': [2759, 3816], 'Thr-460': [2761, 3818], 'changed': [2763], '(AGT': [2769], 'GCT': [2771], 'ACG': [2773], 'GCG,': [2775], 'respectively)': [2776], 'abolish': [2778], 'Asn-77': [2783, 3829], 'Asn-458,': [2785, 3831, 4243], 'All': [2787], 'constructs': [2788], '4)': [2790], 'confirmed': [2796, 3928], 'assay': [2802], 'according': [2805], 'Scholar),': [2840, 4129, 4513], 'al.(29Nishikawa': [2851], '1035:': [2863], '313-318Crossref': [2864], '(113)': [2867], 'substrate': [2871, 4604], 'Gn2(2′,2)core-PA': [2872], 'reported': [2876, 5625], 'previously': [2877], '(30Tokugawa': [2878], '53-56Crossref': [2888], '(53)': [2891], '10': [2894], 'plasmid': [2899], 'incorporated': [2901], '106': [2905], '0.8': [2910], 'ml': [2911], 'phosphate-buffered': [2913], 'saline': [2914], 'electroporation': [2916], 'Gene': [2919], 'Pulser': [2920], '(Bio-Rad)': [2921], 'conditions': [2923], '1,600': [2925], 'V,': [2926], '25': [2927, 3036], 'μF': [2928], 'room': [2930], 'temperature.': [2931], 'h': [2934], 'cultivation': [2936], "Dulbecco's": [2938], 'modified': [2939, 4223], "Eagle's": [2940], 'medium': [2941], 'Inc.)': [2944, 2953], 'supplemented': [2945], 'fetal': [2948], 'calf': [2949], 'serum': [2950], '37': [2955], '°C,': [2956], 'collected,': [2960], 'suspended': [2961], 'μl': [2964], 'buffer': [2966], '(5': [2967], 'mm': [2968, 2975, 4644, 4648, 4652, 4656, 4678], 'Tris-Cl,': [2969], 'pH': [2970], '7.5,': [2971], 'mmMgCl2,': [2973], 'phenylmethylsulfonyl': [2976], 'fluoride),': [2977], 'disrupted': [2979, 3822], 'sonication.': [2981], 'supernatants': [2983], 'collected': [2985], 'centrifugation': [2987], '2,000': [2989], 'g': [2991], 'measure': [2998], 'lysate': [3006], 'normalized': [3008], 'concentration': [3012], 'reflect': [3014], 'number.': [3017], 'b1NPF': [3030], '(5′-CCATAGTGGCAACCAGGATCATC-3′)': [3031], 'b1NPR': [3033], '(5′-CGAAATGGGGTTTAGGCTGG-3′)': [3034], 'primers.': [3035], '25-μl': [3045], 'reaction': [3046], 'kit.': [3051], 'until': [3057], 'difference': [3059, 4326], 'among': [3064], 'became': [3068, 5628], 'obvious.': [3069], '57': [3095], 'Glyceraldehyde': [3112], '3-phosphate': [3113], 'dehydrogenase': [3114], 'primers,': [3119], '5′-CCAAAATCAAGTGGGGCGATG-3′': [3120], '5′-CAGGAGGCATTGCTGATGATCTTG-3′': [3122], 'control.': [3125], 'Poly(A)+': [3126, 3137], 'above.': [3136], 'RNAs': [3138], 'purchased': [3143], 'CLONTECH.': [3145], 'Using': [3146, 5621, 5637], 'obtained': [3155, 3420, 3428, 3541, 3926, 4306, 4343, 4957, 4969, 5062], '31': [3156], 'fractions': [3158, 3198], '(16': [3159], 'fromAchromobacter': [3160], 'digestion': [3163, 4144], '15': [3165], 'treatment).': [3170], 'Twenty': [3171], '(7': [3175], 'former': [3178], '13': [3180], 'latter': [3183], 'treatment)': [3184], 'sequencing,': [3189, 4216], '24': [3191], 'obtained,': [3195], 'since': [3196, 3462], 'gave': [3199, 3874, 5336], 'mixed': [3200], 'signals.': [3201, 3887], 'Four': [3202], 'being': [3208, 5320], 'derived': [3210], 'IgG': [3214, 3238], 'heavy': [3215], 'chain,': [3216], 'whereas': [3217, 3630, 3880, 3983, 5104, 5348, 5432], 'others': [3219], 'novel.': [3221], 'free': [3236], 'NH2-Ile-Leu-Lys-Glu-.': [3241], 'corresponding': [3256, 5076], 'four': [3258, 3300], 'novel': [3259, 3538, 3942], 'sequences,': [3262], 'TableI': [3266], 'Fig.': [3268], '2.': [3269], '0.2-kilobase': [3271], 'sequenced.': [3289], 'encoded': [3295, 5100], 'included': [3299], 'strategy,': [3311], 'indicating': [3312, 3572, 4217, 5298], 'part': [3318], 'screening': [3328, 3351], 'five': [3340], 'independent': [3341], 'φ#1a': [3345], '1e)': [3347], 'they': [3354, 5733], 'shared': [3355, 3988], '3′-terminal': [3357], 'portion': [3358], 'obtain': [3368, 3401], 'initiation': [3370, 3460, 3504], 'screenings': [3377], '5′-terminal': [3380], 'inserts': [3388], 'respectively).': [3395], 'Thus,': [3396], '5′-portion': [3403], 'frame.': [3408], 'two': [3410, 3793, 3933, 5426], 'experiments,': [3413, 3795], 'complete': [3415, 3773, 4297], '(Figs.': [3421, 4995], 'and2).': [3423], '2,246-bp': [3425], 'consisted': [3431], '1,605-bp': [3434], 'frame,': [3437], '287': [3438], '354': [3442], 'regions.': [3445], 'methionine': [3448, 3467], '288–290': [3451, 3476], 'considered': [3455, 4847], 'site,': [3461], 'no': [3463, 3980, 4204, 4274, 4622, 5580], 'found,': [3470], 'around': [3475], 'partially': [3478], 'consistent': [3479], "Kozak's": [3481], 'rule': [3482], '(31Kozak': [3483], 'Nucleic': [3485, 5040], 'Acids': [3486, 5041], '1981;': [3488], '9:': [3489], '5233-5262Crossref': [3490], '(829)': [3493], 'Neither': [3496], 'poly(A)': [3498], 'AATAAA': [3502], 'polyadenylation': [3503], 'found': [3507, 3624], 'within': [3508, 3922, 4839, 4850], '3′-untranslated': [3510], 'obtained.': [3512], 'polypeptide': [3514, 4319], 'consists': [3521], '535': [3523, 3919], 'acids': [3525, 3921], 'expected': [3530], '61,613': [3533], 'Da.': [3534], '19': [3535, 3938], 'appeared': [3547, 4307], '2).': [3554, 4998], 'most': [3564], 'Ile-93,': [3571], 'proteolysis': [3573, 4398], 'purification;': [3576], 'heterogeneity': [3577], 'end': [3580, 3999], 'observed.': [3582], 'Hydrophobicity': [3583], 'surface': [3586], 'probability': [3587], '18-amino': [3592], '(position': [3594], '8–25)': [3595], 'close': [3597], 'domain,': [3606], 'suggesting': [3607, 4015], '(Fig.3).': [3617], 'Potential': [3618], '(Asn-Xaa(except': [3621], 'Pro)-Ser/Thr)': [3622], 'Asn-5,': [3626, 4179], '-77,': [3627], '-458,': [3629], 'O-glycosylation': [3631], 'motif': [3632, 4624], 'preferred': [3633], 'bovineO-GalNAcT1': [3635], '(Thr/Ser-Pro-Xaa-Pro': [3636], 'Thr-Xaa-Xaa-Pro)': [3638], '(32Yoshida': [3639], 'Suzuki': [3641], '16884-16888Abstract': [3652], '(69)': [3660], 'found.': [3665], 'transiently': [3676], 'transfected': [3682], '>50-fold': [3686], 'vitroGnT-IV': [3690], '4,': [3693, 3840, 4819], 'pBGT4).': [3694], 'up': [3697, 3730], 'Ile-93': [3699, 3809, 4007, 4322, 4543, 4828], '(pSigIle93),': [3700], 'identical': [3703], 'still': [3709], 'secreted': [3719], 'sequence.': [3724], 'Pro-113': [3732], '(pSigPro113)': [3733], 'Pro-142': [3735], '(pSigPro142)': [3736], 'mutants': [3746], 'Contrary': [3756], 'expectation,': [3759], 'largest': [3764], 'all': [3767, 3984, 4228, 4612, 5641], 'mutants,': [3771], 'maintained': [3772], 'vitro': [3777, 3957, 4538], '4).': [3779], 'did': [3782, 3833], 'portion,': [3790], 'these': [3792, 5296, 5674, 5713], 'concluded': [3797, 4233], 'must': [3805], 'Amino': [3812], 'substitutions': [3814], 'Ala,': [3820], 'consensus': [3824, 4623, 5032], 'respectively,': [3832], 'significantly': [3835], 'reduce': [3836], 'pS79A': [3841], 'pT460A).': [3843], 'compare': [3851, 4781, 5657], 'abundance': [3854], '(Fig.5).': [3859], 'amount': [3861], 'bands': [3864], 'differed': [3865], 'Small': [3868], 'intestine,': [3869, 4290, 5306], 'kidney,': [3870], 'lung,': [3871], 'spleen': [3873, 5334], 'strong': [3875, 5329, 5338], 'signals': [3876, 4844], 'mRNA,': [3879], 'brain,': [3881], 'heart,': [3882, 5350], 'faint': [3886], 'containing': [3894, 4970], 'encode': [3930], 'methods:': [3934], 'it': [3936, 3953, 4139, 4231, 4264, 4754, 5627, 5652], 'contained': [3937], '2);': [3951], 'conveyed': [3954], 'activityin': [3956], '(Fig.4).': [3964, 4254], 'Comparison': [3965, 5051], 'GnTs': [3978, 3985, 4613, 4632, 4786, 5643, 5663], 'obvious': [3981], 'homology,': [3982], 'rather': [4009], 'hydrophilic': [4010], 'flexible,': [4014], 'stem': [4022, 4048, 4805], 'region.': [4023], 'addition,': [4025], 'lability': [4028], 'exposed.': [4038], 'glycosyltransferases': [4042, 4407, 4519], 'carry': [4043], 'proline-rich': [4044], '(10Sarkar': [4050, 4408], '33Shaper': [4101], 'N.L.': [4102], 'Shaper': [4103], 'J.H.': [4104], 'Meuth': [4105], 'J.L.': [4106, 4108], 'Fox': [4107], 'Chang': [4109], 'Kirsch': [4111], 'I.R.': [4112], 'Hollis': [4113], 'G.F.': [4114], '1986;': [4122, 5515], '83:': [4123], '1573-1577Crossref': [4124], '(161)': [4127], 'not.': [4132], 'N-glycan(s),': [4137], 'because': [4138, 4263], 'sensitive': [4141], 'N-glycosidase': [4143], 'There': [4173, 4272, 4821, 5578], 'sites,': [4178], 'Asn-77,': [4180], 'Asn-458;': [4182], 'however,': [4183], 'lost': [4188], 'Asn-5': [4189, 4259], 'Asn-77.': [4191, 4282], 'Therefore,': [4192, 4753], 'GnT-IV.': [4202, 5636], 'Indeed,': [4203, 4630], 'detected': [4209], 'position': [4213], 'residue': [4220, 5011, 5421], 'might': [4221, 4390, 4552, 4822, 5262], '(data': [4224, 5093], 'shown).': [4226, 5095], 'Taking': [4227], 'evidence': [4229, 4275], 'together,': [4230], 'oligosaccharide': [4241], 'N-glycosylated,': [4262], 'presumably': [4266], 'cytosol': [4270], 'side.': [4271], 'suggest': [4277, 5369], 'glycosylation': [4279], 'During': [4283], 'purification': [4284, 4400], 'inhibitors': [4294], 'avoid': [4300], 'proteolysis.': [4301], 'Nevertheless,': [4302], 'thus': [4305], 'Ile-93.': [4312], 'calculated': [4314], 'molecular': [4315, 4328, 4383], 'weight': [4316, 4329, 4384], 'extending': [4320], '51,000.': [4324], 'above': [4334], 'estimated': [4337], 'size': [4338], '(58,000;': [4348], 'Ref.': [4349], '24Oguri': [4350], 'much': [4379], 'larger': [4380], 'oligosaccharide.': [4388], 'indicate': [4391], 'maturation': [4393], 'thanN-glycosylation.': [4396], 'Limited': [4397], 'Scholar,20Shoreibah': [4474], '34Weinstein': [4492], 'Lee': [4494], 'E.U.': [4495], 'McEntee': [4496], 'Lai': [4498], 'P.H.': [4499], 'Paulson': [4500], 'J.C.': [4501], '17735-17743Abstract': [4507], 'suggests': [4515], 'truncations': [4517], 'occur': [4520], 'naturally': [4521, 4945], 'functions.': [4526, 5382], 'N-': [4527], 'indicated': [4532], 'bearing': [4536], 'lysines': [4550], 'involved': [4554, 5400, 5647], 'UDP': [4556], 'binding': [4557, 4605, 4765], '(35Yadav': [4558], 'Brew': [4560], '265:': [4566], '14163-14169Abstract': [4567], '36Kazuta': [4574], 'Tanizawa': [4576], 'Fukui': [4578], '110:': [4583], '708-713Crossref': [4584], '(10)': [4587], 'So': [4590], 'far,': [4591], 'there': [4592, 4620, 5065], 'little': [4594], 'regarding': [4596, 4952], 'tertiary': [4598], 'GnTs,': [4601], 'specified.': [4610], 'share': [4614], 'donor': [4617, 4767], 'substrate,': [4618], 'UDP-GlcNAc,': [4619], 'sequences.': [4629], 'so': [4634], 'far': [4635], 'quite': [4637], 'differentK': [4638], 'm': [4639], 'values': [4640], 'UDP-GlcNAc': [4642], '(0.04': [4643], '0.96': [4647], '3.1': [4651], '0.22': [4655], '2S.': [4659], 'Oguri,': [4660], 'Minowa,': [4663], 'Ihara,': [4665], 'Taniguchi,': [4667], 'Ikenaga,': [4669], 'Takeuchi,': [4672], 'unpublished': [4673], 'observation.': [4674], ',': [4675], '11': [4677], 'GnT-V)': [4680], '(20Shoreibah': [4681], '37Nishikawa': [4699], 'Pegg': [4701], 'W.': [4702, 4899], 'Paulsen': [4703], '263:': [4711], '8270-8281Abstract': [4712], '38Bendiak': [4719], '5784-5790Abstract': [4728], '39Nishikawa': [4735], 'Sugiyama': [4739], 'Anal.': [4743], '170:': [4746], '349-354Crossref': [4747], '(59)': [4750], 'different': [4762, 4971, 5290], 'means': [4763], 'substrate.': [4768], 'would': [4770, 4931], 'like': [4771], 'elucidate': [4773, 5697], 'function': [4777], 'when': [4787], 'available.': [4788], 'Our': [4789], 'attempt': [4790], 'soluble': [4793], 'forms': [4794], 'replacement': [4800], 'regions': [4806, 4856], 'enough': [4814], 'full': [4816], 'secretion': [4817], 'pSigIle93).': [4820], 'retain': [4836], 'cell,': [4841], 'usually': [4846], 'lie': [4849], 'nearby': [4855], '(40Matsuoka': [4857], 'Chen': [4859], 'S.Y.': [4860], 'Compans': [4861], 'R.W.': [4862], '269:': [4867], '22565-22573Abstract': [4868], '41Hobman': [4875], 'T.C.': [4876], 'Woodward': [4877], 'L.': [4878], 'Farquhar': [4879], 'Mol.': [4881], 'Cell.': [4883], '6:': [4885], '7-20Crossref': [4886], '(44)': [4889], '42Tang': [4892], 'B.L.': [4893], 'Low': [4894], 'S.H.': [4895, 4897], 'Wong': [4896, 4898], '66:': [4905], '365-374PubMed': [4906], '43Munro': [4909], 'EMBO': [4911], '4695-4704Crossref': [4915], '(342)': [4918], 'Currently,': [4921], 'attempting': [4924], 'raise': [4926], 'antibody': [4927], 'against': [4928], 'help': [4932], 'localization': [4936], 'cells,': [4941], 'well': [4943, 5318, 5619], 'occurring': [4946], 'Partial': [4950], 'information': [4951], 'intron/exon': [4954], 'organization': [4955], 'experiments.': [4961], 'total': [4963], 'fragments.': [4973], '#3a': [4977, 5024], '#4b': [4979, 5026], 'gaps': [4981, 5021], '61': [4987, 4991], '381': [4989], '185,': [4993], 'respectively': [4994], 'hand': [5002], 'continuous': [5007], 'starting': [5009], '34.': [5012], 'Complementary': [5013], 'preceding': [5016], 'ends': [5018], 'intron': [5036], 'acceptors': [5037], '(44Mount': [5038], 'S.M.': [5039], '1982;': [5043], '10:': [5044], '459-472Crossref': [5045], '(2784)': [5048], 'segments': [5061], 'least': [5068], 'introns': [5070], '1–550': [5078], 'splicing': [5086], 'occurred': [5089], 'GnT-I': [5096], '-II': [5098], 'single': [5102], 'exons,': [5103], 'GnT-III': [5105, 5417], 'complex': [5109], 'organizations': [5111], '(11Hull': [5112], 'exons/introns,': [5259], 'feature': [5266], 'branch-forming': [5271], 'forN-glycans.': [5273], 'result': [5288], 'levels': [5291, 5660], 'tissues,': [5297], 'regulated.': [5303], 'source': [5312, 5322], 'purification,': [5326], 'signal.': [5332, 5360], 'signal,': [5339], 'coincident': [5342], 'activity,2': [5347], 'undetectable': [5354], 'activity,2produced': [5357], 'weak': [5359], 'tissue': [5362], 'specific': [5363], 'pattern': [5365], 'importance': [5371], 'role': [5373, 5609], 'be,': [5388], 'part,': [5390], 'controlled': [5391], 'relative': [5394], 'activities': [5395], 'formation,': [5403], '-IV,': [5407], '-V,': [5408], 'galactosyltransferase.': [5411], 'bisecting': [5413], 'galactose': [5420], 'attached': [5422, 5727], 'either': [5424], 'β1–2': [5427], 'GlcNAcs': [5428], 'inhibited': [5429], 'action,': [5431], 'mannose,': [5439], 'enhanced': [5446], 'tumor': [5497], 'mainly': [5500], 'due': [5501], 'elevation': [5504], 'GnT-V': [5506, 5567], '(45Pierce': [5508], 'Arango': [5510], '261:': [5516], '10772-10777Abstract': [5517], '46Hubbard': [5524], 'S.C.': [5525], '16403-16411Abstract': [5531], '47Asada': [5538], 'Furukawa': [5540], 'Segawa': [5542], 'Cancer': [5546], '57:': [5549], '1073-1080PubMed': [5550], 'Since': [5553], 'together': [5565], 'contribute': [5569], 'changes': [5572], 'require': [5574], 'specifically.': [5577], 'immunologically': [5589], 'Man.': [5601], 'reason': [5606], 'phenomena': [5616], 'understood.': [5620], 'here,': [5626], 'evaluate': [5631], 'galactosyltransferase': [5646], 'N-glycan': [5649], 'will': [5653, 5676, 5692, 5716], 'clarify': [5665], 'control': [5667], 'branching.': [5669], 'Overexpression': [5670], 'suppression': [5672], 'provide': [5677], 'tools': [5678], 'producing': [5680], 'glycoproteins': [5681, 5729], 'targeted': [5683], 'antennary': [5684], 'structures.': [5687], 'Also,': [5688], 'approach': [5691], 'now': [5693], 'available': [5695], 'factors': [5699], 'N-glycans': [5706, 5726], 'GnT-I–V': [5710], 'enzymes.': [5711], 'Moreover,': [5712], 'useful': [5719], 'remodel': [5721], 'see': [5731], 'if': [5732], 'alter': [5734], 'properties': [5737], 'proteins.': [5740], 'grateful': [5744], 'Prof.': [5746], 'Naoyuki': [5747], '(Osaka': [5749], 'Univ.)': [5750], 'helpful': [5752], 'discussions.': [5753], 'thank': [5756], 'Dr.': [5757], 'Shinji': [5758], 'Takamatsu': [5759], '(KIRIN': [5760], 'Brewery)': [5761], 'critical': [5763], 'manuscript.': [5767]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2017214357', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 5}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 6}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-14T03:15:13.824635', 'created_date': '2016-06-24'}
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