Title: Observations on the specificity of thermolysin with synthetic peptides
Abstract: It was found previously that thermolysin, a thermostable protease (Endo, 1962, 1965), hydrolysed preferentially peptide bonds involving the amino groups of hydrophobic amino acid residues with bulky side chains in several protein substrates (Matsubara et al., 1965, Matsubara et al., 1966. In the present study, several synthetic peptides were tested as substrates for thermolysin. Only one type of peptide, exemplified by Cbz (Carbobenzoxy)-Gly-Pro-Leu-Ala-Pro, which has been used as a substrate for collagenase (Nagai, etal., 1960), has previously been found to be a good substrate for thermolysin (Ohta and Ogura, 1965). In the searching for simpler substrates it was found that Cbz-Gly-L-Phe-amide and Cbz-Ala-L-Leu-hydrazide were readily hydrolysed by thermolysin. The present communication compares several synthetic peptides and presents a discussion of the structural requirement for their susceptibility to hydrolysis by this enzyme.
Publication Year: 1966
Publication Date: 1966-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 69
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