Title: NGF-Induced Stabilization of Gap-43 mRNA Is Mediated by Both 3′ Untranslated Region and a Segment Encoding the Carboxy-Terminus Peptide
Abstract: NGF stabilizes cytoplasmic mRNA for GAP-43, a neuronal growth cone protein. A 292 b segment of rat GAP-43 mRNA was determined to mediate this stabilizing effect. Insertion of the 292 b segment into heterologous mRNA was also shown to constitutively destabilize the mRNA in the absence of NGF. This 292 b segment contains the sequences encoding the carboxy-terminus peptide and part of the 3′ untranslated region. Neither one of these sequences can fully mediate the NGF-dependent stability control independently from each other. Premature termination of translation before the 292 b segment reduces both the constitutive destabilization by the 292 b segment and the NGF-induced stabilization. These findings indicate that the constitutive destabilizing function of the 292 b segment is dependent on the direct translation of this segment and that NGF suppresses the destabilizing function of this segment.
Publication Year: 1994
Publication Date: 1994-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 20
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