Title: Model study on the fate of lipophilic materials incorporated into sodium dodecyl sulfate-protein polypeptide complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Abstract: Complexes formed between sodium dodecyl sulfate and polypeptides, derived from proteins by reductive cleavage of disulfide crosslinkages and denaturation, are the electrophoresing entities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sodium dodecyl sulfate bound to the polypeptides has something in common with sodium dodecyl sulfate constituting micelles, and can incorporate lipophilic materials (T. Takagi, K. Kubo, and T. Isemura, 1980, Biochim. Biophys. Acta, in press). Lipophilic materials often contaminating samples of sodium dodecyl sulfate-polyacrylamide gel electrophoresis are thus presumed to be incorporated into the complexes. Model experiments using an oil-soluble dye, 1-o-tolylazo-2-naphthylamine, were carried out to confirm the above assumption, and to follow the fate of such materials during the electrophoresis. The dye incorporated into the complexes was found to be turned over to sodium dodecyl sulfate micelles migrating from the upper buffer reservoir through the gel and overtaking the complexes. The complexes are thus continuously deprived of the dye. A large-sized complex is free of the dye more promptly than a small-sized complex, because the former is electrophoresed more slowly and is overtaken by more sodium dodecyl sulfate micelles than the latter. The dye is retained in the complexes only when the overtaking micelles have been also saturated with the dye.
Publication Year: 1980
Publication Date: 1980-06-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot