Title: Characterization of macromolecule resonances in the <sup>1</sup>H NMR spectrum of rat brain
Abstract: Abstract The 1 H NMR spectrum of the macromolecule fraction of rat brain cytosol was investigated following centrifugation and dialysis to remove low molecular weight metabolites and peptides (<3500 daltons). At least seven well resolved resonances were detected between 0.9 and 3.0 ppm in the 1 H NMR spectrum of rat brain cytosol after dialysis, several of which cannot be observed in vivo due to overlap with N ‐acetylaspartate, glutamate, glutamine, creatine, and yaminobutyric acid. Several cross‐peaks detected in 2D COSY spectra of the cytosolic macromolecule fraction coincided with those measured in a previous study of rat brain tissue in vitro and in situ (K. L. Behar, T. Ogino, Magn. Reson. Med. 17, 285 (1991)). Treatment of the cytosolic macromolecule fraction with a nonspecific protease permitted partial assignments of resonances in the 1 H NMR spectrum to specific amino acids. Fractionation of the dialyzed cytosol of rat brain by gel filtration yielded qualitatively similar 1 H NMR spectra for elution volumes corresponding to molecular masses from 12.5 kDa to over 100 kDa. The results indicate that many of the background nonmetabolite resonances observed in the 1 H NMR spectrum of normal brain tissue arise from cytosolic proteins.
Publication Year: 1993
Publication Date: 1993-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 208
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