Title: The inactivation of foot-and-mouth disease virus at ionic-strength dependent isoelectric points
Abstract: Foot-and-mouth disease virus (FMDV) isoelectric point (pI) values, determined by cellulose acetate electrophoresis, were linear with respect to the square root of the ionic strength. This effect for proteins is attributed to the preferential binding of anions. The Longsworth and Jacobsen equation, pI=pI0−(AΓ/2)/(1+BΓ/2), was evaluated for FMDV; Γ/2 is the ionic strength, pI0 is the isoionic point 7.6, and constants A and B are 21.5 and 4.2, respectively. With pI values of 3.25−7.5 estimated from this equation, it was found that FMDV was always inactivated at its pl, while at 2 pH units above its pI there was no loss of infectivity. Storing virus at conditions greater than 2 pH units above isoelectric points at alkaline pH also resulted in inactivation. Thus, virus inactivation or stability can be predetermined over a range of hydrogen ion concentrations and ionic strengths. Inactivation of FMDV below pH 7.0 in isotonic solution or degradation at pH 7.5 in hypotonic solution (Γ/2 0.002) which previously appeared to occur by two independent mechanisms are both the result of isoelectric inactivation.
Publication Year: 1967
Publication Date: 1967-03-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 12
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