Enzymatic and Molecular Characteristics of the Efficiency and Specificity of Exfoliative Toxin Cleavage of Desmoglein 1

Name: Work Video:
Duration: 3 min 30 s
Yasushi Hanakawa; Norman M. Schechter; Chenyan Lin; Koji Nishifuji; Masayuki Amagai; John R. Stanley
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'https://openalex.org/W313645322', 'https://openalex.org/W4238016904', 'https://openalex.org/W4242095212', 'https://openalex.org/W567590274'], 'related_works': ['https://openalex.org/W4213199728', 'https://openalex.org/W3012348287', 'https://openalex.org/W2954791979', 'https://openalex.org/W2165108698', 'https://openalex.org/W2092969107', 'https://openalex.org/W2075320741', 'https://openalex.org/W2069739887', 'https://openalex.org/W2015791575', 'https://openalex.org/W2014791279', 'https://openalex.org/W1824702637'], 'abstract_inverted_index': {'Exfoliative': [0, 190], 'toxins': [1, 191, 448], '(ETs)': [2, 192, 449], 'from': [3, 128, 193, 318, 1499, 1968, 2519, 2853, 3007, 3516, 3659, 3757, 4001, 4277, 4293, 4443], 'Staphylococcus': [4, 194, 380], 'aureus': [5, 195, 381, 400, 443, 713, 1747], 'blister': [6, 196, 719, 742], 'the': [7, 33, 42, 50, 86, 138, 161, 171, 176, 197, 223, 232, 240, 276, 328, 351, 361, 366, 403, 518, 609, 652, 829, 854, 886, 889, 1008, 1063, 1068, 1129, 1236, 1328, 1381, 1492, 1514, 1553, 1568, 1581, 1586, 1590, 1611, 1670, 1675, 1678, 1686, 1692, 1695, 1702, 1863, 1925, 2121, 2132, 2188, 2200, 2215, 2335, 2372, 2439, 2469, 2520, 2557, 2600, 2616, 2621, 2644, 2682, 2688, 2765, 2854, 2955, 2982, 3004, 3025, 3034, 3040, 3052, 3064, 3212, 3218, 3227, 3425, 3444, 3541, 3575, 3591, 3623, 3641, 3653, 3687, 3696, 3704, 3758, 3811, 3817, 3834, 3846, 3884, 3895, 3908, 3924, 3938, 3950, 3955, 3962, 4002, 4008, 4074, 4079, 4096, 4117, 4131, 4141, 4145, 4160, 4169, 4184, 4198, 4294, 4312, 4323, 4352, 4368, 4396, 4419], 'superficial': [8, 198, 519], 'epidermis': [9, 199, 520], 'by': [10, 54, 72, 78, 200, 244, 262, 268, 398, 441, 541, 711, 900, 1140, 1376, 1394, 1434, 1522, 1617, 1622, 1800, 1934, 2045, 2059, 2079, 2214, 2295, 2434, 2514, 2611, 2784, 2900, 2966, 3037, 3104, 3128, 3168, 3199, 3350, 3450, 3456, 3477, 3519, 3540, 3588, 3615, 3646, 3677, 3695, 3898, 3932, 4028, 4042, 4053, 4107, 4211, 4271, 4341, 4405, 4455], 'hydrolyzing': [11, 201, 1485], 'a': [12, 180, 185, 188, 202, 370, 375, 378, 383, 542, 776, 822, 1307, 1475, 1594, 1597, 1630, 1879, 1918, 1931, 2017, 2070, 2184, 2192, 2637, 3223, 3459, 3474, 3523, 3555, 3596, 3671, 3750, 3793, 4135, 4227, 4328], 'single': [13, 203, 1476, 3475], 'peptide': [14, 204, 468, 641, 1319, 1454, 1477, 1487, 1673, 1987], 'bond,': [15, 205], 'Glu381–Gly382,': [16, 206], 'located': [17, 207], 'between': [18, 110, 208, 300, 1145, 1335, 2371, 2405, 2599, 2832, 2905, 2915, 4236, 4258], 'extracellular': [19, 133, 209, 323, 633, 1084], 'domains': [20, 210, 1082, 1086, 1147, 1506, 2374, 2559, 2602, 3999, 4020, 4037, 4218, 4244], '3': [21, 135, 211, 325], 'and': [22, 57, 107, 113, 164, 212, 247, 297, 303, 354, 456, 459, 470, 474, 551, 611, 626, 646, 732, 751, 834, 838, 909, 914, 1323, 1337, 1439, 1445, 1504, 1571, 1602, 1681, 1688, 1790, 1811, 1866, 1884, 1899, 1903, 1920, 1980, 2030, 2042, 2074, 2190, 2293, 2301, 2369, 2377, 2404, 2407, 2436, 2471, 2595, 2605, 2613, 2623, 2639, 2720, 2834, 2842, 2873, 2884, 2908, 2920, 2940, 3022, 3049, 3085, 3298, 3332, 3354, 3467, 3493, 3499, 3504, 3509, 3547, 3560, 3581, 3590, 3606, 3618, 3648, 3661, 3684, 3726, 3775, 3784, 3805, 3822, 3824, 4058, 4172, 4220, 4238, 4260, 4387, 4390, 4410, 4428, 4447], '4': [23, 213, 3315, 4180, 4193], 'of': [24, 36, 44, 52, 62, 104, 137, 166, 175, 214, 226, 234, 242, 252, 294, 327, 356, 365, 402, 420, 432, 517, 524, 544, 608, 843, 892, 1007, 1017, 1067, 1088, 1133, 1239, 1374, 1495, 1552, 1563, 1573, 1577, 1585, 1627, 1677, 1694, 1874, 1906, 1913, 2027, 2065, 2072, 2120, 2127, 2134, 2218, 2281, 2375, 2530, 2560, 2603, 2634, 2677, 2780, 2829, 2838, 2902, 2912, 2937, 2957, 3003, 3018, 3028, 3060, 3063, 3078, 3115, 3132, 3154, 3173, 3185, 3281, 3372, 3438, 3441, 3446, 3448, 3461, 3481, 3502, 3522, 3543, 3598, 3652, 3707, 3731, 3752, 3760, 3762, 3772, 3792, 3814, 3819, 3837, 3863, 3872, 3887, 3910, 3923, 3940, 3954, 3965, 4004, 4011, 4035, 4046, 4071, 4076, 4086, 4114, 4116, 4133, 4149, 4273, 4297, 4315, 4327, 4351, 4371, 4414, 4439], 'desmoglein': [25, 215, 472, 624, 644, 859], '1': [26, 216, 458, 473, 625, 645, 860, 1997, 2011, 2114, 2228, 2232, 2291, 2773, 3318, 3367, 3410, 3485, 3617, 3683], '(Dsg1).': [27, 217], 'Enzyme': [28, 218], 'activity': [29, 219, 3836], 'is': [30, 220, 382, 439, 561, 894, 1061, 1121, 1138, 1229, 1378, 1497, 3101, 3532, 3570, 3692, 3709, 3749, 3904, 3945, 3959, 4026, 4065, 4082, 4121, 4213, 4382, 4402, 4453], 'dependent': [31, 221, 1379, 3948, 3960, 4215], 'on': [32, 170, 222, 360, 1380, 1580, 1854, 1885, 1924, 1973, 2325, 2643, 3211, 3222, 3554, 3949, 3961, 4126, 4144, 4216], 'calcium-stabilized': [34, 224, 1227, 1383, 3963], 'structure': [35, 225, 1132, 1228, 4314], 'Dsg1.': [37, 156, 227, 346, 477, 650, 895, 1461], 'Here': [38, 228], 'we': [39, 120, 229, 310, 1465, 1525, 3452, 3934, 4030, 4232, 4377], 'further': [40, 230, 4241, 4361], 'define': [41, 231, 4362], 'characteristics': [43, 233], 'this': [45, 235, 741, 1463, 4150, 4167, 4437], 'cleavage.': [46, 236, 4069, 4205, 4266], 'Kinetic': [47, 237], 'studies': [48, 238, 1300, 1565], 'monitoring': [49, 239], 'cleavage': [51, 88, 177, 241, 278, 367, 1373, 1515, 1521, 1554, 1576, 1587, 2066, 2777, 2828, 2899, 2965, 3026, 3035, 3053, 3065, 3127, 3167, 3198, 3228, 3455, 3525, 3791, 3956, 4041, 4089, 4115, 4132, 4158, 4185, 4210, 4305, 4397, 4424], 'Dsg1': [53, 106, 112, 129, 243, 296, 302, 319, 916, 1060, 1311, 1325, 1375, 1390, 1508, 1533, 1578, 1916, 2298, 3449, 3944, 3966, 4246, 4278, 4356, 4389], 'ETA,': [55, 245, 1788, 2028, 3129, 3465, 3782, 4095], 'ETB,': [56, 246, 908, 1789, 2029, 3466, 3783], 'ETD': [58, 248, 1612, 1679, 1696, 2031, 3468], 'demonstrated': [59, 249], 'kcat/Km': [60, 250], 'values': [61, 251, 3745, 3788], '2–6': [63, 253], '×': [64, 254], '104m–1': [65, 255], 's–1,': [66, 256], 'suggesting': [67, 84, 257, 274, 2953, 2976], 'very': [68, 181, 258, 371, 3041], 'efficient': [69, 259, 1483, 3790], 'proteolysis.': [70, 260], 'Proteolysis': [71, 261], 'ETA': [73, 149, 167, 263, 339, 357, 1496, 1529, 1574, 1671, 2145, 2230, 2272, 2775, 2901, 3496, 3531, 3864, 3888, 4054, 4155, 4212, 4342, 4406], 'was': [74, 264, 745, 761, 826, 898, 1615, 1663, 1699, 1742, 1928, 1966, 2005, 2022, 2067, 2117, 2212, 2240, 2275, 2304, 2512, 2562, 2647, 2679, 2725, 2770, 2782, 2951, 2973, 3407, 3488, 3840, 4138, 4175, 4200, 4269], 'not': [75, 153, 265, 343, 807, 919, 1122, 1302, 1511, 1537, 2944, 2974, 2980, 2998, 3102, 3141, 3143, 3878, 3946, 4066, 4214, 4307, 4403], 'efficiently': [76, 266, 3854], 'inhibited': [77, 267], 'broad': [79, 269, 3827], 'spectrum': [80, 270, 3828], 'serine': [81, 271, 755, 1011, 1025, 1428, 1501, 1803, 2138, 3829, 3900], 'protease': [82, 272, 1804, 2225, 2236, 3830, 3851, 3857, 3901], 'inhibitors,': [83, 273], 'that': [85, 122, 160, 275, 312, 350, 445, 569, 708, 725, 747, 763, 769, 828, 888, 902, 1022, 1094, 1143, 1304, 1314, 1467, 1486, 1491, 1505, 1527, 1549, 1567, 2695, 2866, 2933, 2954, 2977, 3097, 3113, 3217, 3883, 3937, 3998, 4063, 4190, 4209, 4254, 4288], 'enzyme': [87, 277, 2209, 3753], 'site': [89, 178, 279, 368, 1494, 1516, 1555, 1588, 3027, 3036, 3229, 3886, 3957, 4003, 4398], 'may': [90, 168, 280, 358, 3889, 4224], 'be': [91, 281, 808, 1232, 1392, 3890, 4102, 4321, 4339], 'inactive': [92, 282, 2136, 3906], 'or': [93, 117, 283, 307, 778, 926, 1127, 1387, 2231, 2248, 2254, 2273, 2319, 3292, 3390, 3432, 3497, 3630, 3722, 3903], 'inaccessible': [94, 284], 'before': [95, 285], 'specific': [96, 182, 286, 372, 1473], 'binding': [97, 173, 287, 363, 1142, 1583], 'to': [98, 288, 554, 724, 754, 772, 810, 841, 885, 1075, 1231, 1470, 1513, 1531, 1558, 1599, 1666, 1674, 1682, 1691, 1807, 1862, 2143, 2306, 2465, 2474, 2619, 2626, 2691, 2717, 2727, 2898, 2964, 3012, 3126, 3166, 3179, 3226, 3357, 3550, 3574, 3611, 3620, 3736, 3767, 3832, 3861, 3892, 3942, 4023, 4051, 4124, 4177, 4204, 4240, 4385], 'its': [99, 289, 1520, 1605, 3124, 3164, 3911, 4423], 'substrate.': [100, 290, 3796, 3913], 'Using': [101, 291], 'truncated': [102, 292, 2311, 2593, 3083, 4044, 4080], 'mutants': [103, 293, 2693], 'human': [105, 111, 115, 295, 301, 305, 388, 643, 915, 1322, 1914, 2378, 3089, 3161, 3180, 4386], 'chimeric': [108, 298, 2365, 2597, 2830, 2839, 2867], 'molecules': [109, 299, 922, 2313, 2366, 2598, 2831, 2868, 2904, 2914, 4045], 'either': [114, 304, 775], 'Dsg3': [116, 306, 925, 2379, 4280], 'canine': [118, 145, 155, 308, 335, 345, 649, 1532, 2521], 'Dsg1,': [119, 309, 1134, 1480, 3021, 3048, 4298, 4449], 'show': [121, 311, 836, 1301, 1306, 1449, 1466, 1490, 1526, 3073, 3875, 4208], 'for': [123, 313, 577, 1310, 1452, 1474, 1519, 2032, 2056, 2131, 2187, 2206, 2264, 2290, 2556, 2776, 2962, 2986, 3197, 3302, 3317, 3366, 3409, 3600, 3627, 3746, 3781, 3929, 4040, 4048, 4068, 4265, 4304, 4359, 4375], 'cleavage,': [124, 314, 4077, 4376], 'human-specific': [125, 315], 'amino': [126, 316, 1091, 1436, 1547, 2697, 2849, 3014, 3056, 3069, 3117, 3134, 3156, 3170, 3187, 3951, 4181, 4194, 4290, 4318, 4365, 4393, 4434], 'acids': [127, 317, 1548, 2698, 3070, 3157, 3188, 4182, 4195, 4291, 4319, 4366, 4394, 4435], 'are': [130, 144, 320, 334, 392, 1024, 1095, 1432, 1482, 1517, 1550, 1556, 3221, 4247, 4262, 4373], 'necessary': [131, 321, 1518, 2983, 4039, 4067], 'in': [132, 187, 322, 377, 387, 408, 531, 537, 549, 571, 721, 727, 831, 849, 1012, 1062, 1234, 1321, 1456, 1468, 1479, 1484, 1507, 1575, 1596, 1988, 2035, 2221, 2267, 2283, 2722, 2764, 3045, 3071, 3121, 3138, 3158, 3189, 3192, 3208, 3243, 3344, 3369, 3377, 3404, 3422, 3470, 3534, 3665, 3699, 3702, 3894, 3907, 4226, 4245, 4256, 4311, 4322, 4355, 4367, 4436, 4445], 'domain': [134, 324, 1119, 1912, 2633, 4098, 4234, 4296], 'upstream': [136, 174, 326, 364, 1551, 1584, 3062], 'scissile': [139, 329], 'bond.': [140, 330, 1488], 'If': [141, 331], 'these': [142, 332, 1013, 1564, 3471, 4036, 4127, 4192, 4275, 4317], 'residues': [143, 333, 2851, 2871, 2875, 3119, 3136, 3177], 'rather': [146, 336], 'than': [147, 337, 565, 2198], 'human,': [148, 338], 'binds,': [150, 340], 'but': [151, 341, 918, 1312, 1510, 1540, 2879, 3001, 3130, 3920, 3958], 'does': [152, 342, 1536, 2979, 2997], 'cleave,': [154, 344], 'These': [157, 347, 2052, 2329, 3787, 3880, 3995, 4060, 4206, 4251], 'data': [158, 348, 3592, 3663, 3688, 3881, 4061, 4207, 4252], 'suggest': [159, 349, 1566, 3882, 3997, 4253], 'exquisite': [162, 352, 1450, 1569], 'specificity': [163, 353, 1309, 1451, 1570], 'efficiency': [165, 355, 1572, 3445, 4010, 4075], 'depend': [169, 359, 1579], "enzyme's": [172, 362, 1582], 'with': [179, 369, 395, 729, 1589, 1740, 1809, 1878, 1897, 1917, 2016, 2024, 2048, 2146, 2242, 2278, 2316, 2636, 2681, 2712, 2772, 2787, 2862, 2929, 2935, 3088, 3109, 3176, 3287, 3296, 3309, 3325, 3335, 3385, 3396, 3418, 3429, 3490, 3528, 3562, 3572, 3741, 4094, 4104, 4154, 4159], 'fit,': [183, 373, 1592], 'like': [184, 374, 1135, 1593, 3099, 4451], 'key': [186, 376, 1595], 'lock.': [189, 379], 'frequent': [384], 'bacterial': [385], 'pathogen': [386], 'disease.': [389], 'Skin': [390], 'infections': [391], 'particularly': [393], 'prevalent,': [394], 'impetigo': [396, 433, 731, 833], 'caused': [397, 440, 718, 1015], 'S.': [399, 442, 479, 498, 582, 712, 1171, 1192, 1194, 1212, 1746, 1950, 2351, 2392, 2413, 2452, 2486, 2535, 2663, 2746], 'one': [401, 1318, 1453, 1457], 'most': [404], 'common': [405, 564], 'infectious': [406], 'diseases': [407], 'children': [409], '(1Darnstadt': [410], 'G.L.': [411], 'Behrman': [412], 'R.E.': [413], 'Kliegman': [414], 'R.': [415, 1149, 1269, 2482, 2484, 2537], 'Jenson': [416], 'H.B.': [417], 'Nelson': [418], 'Textbook': [419], 'Pediatrics.': [421], 'W.': [422, 614], 'B.': [423, 615, 1190], 'Saunders': [424, 616], 'Co.,': [425, 617], 'Philadelphia,': [426, 618], 'PA2000:': [427], '2028-2030Google': [428], 'Scholar).': [429, 512, 621, 803, 881, 1005, 1059, 1115, 1225, 1298, 1371, 1423, 1661, 1738, 1782, 1849, 1961, 2113, 2180, 2362, 2554, 2590, 2674, 2757, 3994], 'Approximately': [430], '30%': [431], 'patients': [434, 728], 'develop': [435], 'bullous': [436, 514, 566, 730, 832], 'impetigo,': [437, 515, 567], 'which': [438, 538, 758, 850, 1440, 2211, 3703, 3748, 4120, 4243, 4363, 4433], 'strains': [444], 'produce': [446, 555], 'exfoliative': [447, 622, 3029], '1The': [450], 'abbreviations': [451], 'used': [452, 1665, 1785, 2305, 2726, 3300, 3469, 4233, 4378], 'are:': [453], 'ETexfoliative': [454], 'toxinDsg1': [455], '-3desmoglein': [457], '3,': [460, 475, 627, 647, 4056], 'respectivelySSSSstaphylococcal': [461], 'scalded': [462, 533, 630], 'skin': [463, 534, 631, 722], 'syndromeECextracellularTBSTris-buffered': [464], 'salineDFPdiiopropylfluorophosphateDCI3,4-dichloroisocoumarinGSTglutathione': [465], 'S-transferaseE': [466], 'tagE': [467], 'taghDsg1': [469], '-3human': [471], 'respectivelycDsg1canine': [476], '(2Ladhani': [478, 581], 'Joannou': [480, 583], 'C.L.': [481, 584], 'Lochrie': [482, 585], 'D.P.': [483, 586], 'Evans': [484, 587], 'R.W.': [485, 588], 'Poston': [486, 589], 'S.M.': [487, 590], 'Clin.': [488, 499, 591, 1050, 1362, 1729, 1773, 1840, 2171, 2817, 3270, 4480], 'Microbiol.': [489, 500, 592], 'Rev.': [490, 593, 1287], '1999;': [491, 594], '12:': [492, 595], '224-242Crossref': [493, 596], 'PubMed': [494, 510, 597, 669, 686, 702, 799, 877, 943, 971, 1001, 1055, 1113, 1161, 1182, 1204, 1221, 1259, 1277, 1294, 1367, 1419, 1657, 1734, 1778, 1845, 2109, 2176, 2429, 2507, 2550, 2586, 2822, 3275, 3990, 4485], 'Google': [495, 511, 598, 672, 689, 705, 802, 880, 946, 974, 1004, 1058, 1114, 1164, 1185, 1207, 1224, 1262, 1280, 1297, 1370, 1422, 1660, 1737, 1781, 1848, 1960, 2112, 2179, 2361, 2402, 2430, 2462, 2510, 2553, 2589, 2673, 2756, 2825, 3278, 3993, 4488], 'Scholar,': [496, 599, 673, 690, 947, 975, 1165, 1186, 1208, 1263, 1281], '3Ladhani': [497], 'Infect.': [501, 681, 996, 1652], '2001;': [502, 1110], '7:': [503], '301-307Abstract': [504], 'Full': [505, 507, 666, 966, 968, 1274, 1414, 1416, 2104, 2106, 2426, 2502, 2504, 3985, 3987], 'Text': [506, 508, 667, 967, 969, 1275, 1415, 1417, 2105, 2107, 2427, 2503, 2505, 3986, 3988], 'PDF': [509, 668, 970, 1276, 1418, 2108, 2428, 2506, 3989], 'In': [513, 651, 1462, 4166, 4283], 'blisters': [516, 830], 'occur': [521, 530, 570], 'at': [522, 757, 1326, 1795, 2039, 2139, 2191, 2261, 2287, 3314, 3339, 3412, 3716, 3845, 3858], 'sites': [523], 'infection.': [525], 'More': [526], 'extensive': [527], 'disease': [528, 848], 'can': [529, 1441, 4099], 'staphylococcal': [532, 629], 'syndrome': [535, 632], '(SSSS),': [536], 'ETs': [539, 709, 739, 893, 1014, 1305, 1377, 1448, 1481, 1851, 1875, 3457, 3941, 4456], 'produced': [540, 710, 1929, 2433, 2610, 2648], 'nidus': [543], 'infection': [545], 'circulate': [546], 'systemically,': [547], 'usually': [548], 'infants': [550], 'young': [552], 'children,': [553], 'more': [556, 1426], 'generalized': [557], 'blistering.': [558], 'Although': [559, 4309], 'SSSS': [560, 835], 'much': [562], 'less': [563, 2197], 'outbreaks': [568], 'neonatal': [572, 716], 'nurseries': [573], 'have': [574, 749, 3935, 4015], 'important': [575, 2960], 'consequences': [576], 'health': [578], 'care': [579], 'delivery': [580], '4Shinefield': [600], 'H.R.': [601], 'Remington': [602], 'J.S.': [603], 'Klein': [604], 'J.O.': [605], 'Infectious': [606], 'Diseases': [607], 'Fetus': [610], 'Newborn': [612], 'Infants.': [613], 'PA1995:': [619], '1105-1141Google': [620], 'toxin': [623], 'respectively': [628, 648], 'Tris-buffered': [634], 'saline': [635], 'diiopropylfluorophosphate': [636, 2245], '3,4-dichloroisocoumarin': [637, 2251], 'glutathione': [638], 'S-transferase': [639, 2322], 'E': [640, 1921, 1985, 2317, 2320, 2470, 2622, 2640, 3304, 3381, 4118, 4142, 4171], 'tag': [642, 1922, 1976, 1986, 2641, 2789, 2864, 2931, 3093, 3111, 3382, 4106, 4137, 4143, 4174], '1970s': [653], 'Melish': [654], 'et': [655], 'al.': [656], '(5Melish': [657], 'M.E.': [658, 675, 692], 'Glasgow': [659, 676, 693], 'L.A.': [660, 677, 694], 'J.': [661, 680, 697, 960, 1049, 1107, 1169, 1196, 1200, 1216, 1267, 1361, 1408, 1728, 1772, 1839, 1955, 2098, 2170, 2356, 2397, 2420, 2457, 2496, 2668, 2751, 2816, 3269, 3979, 4479], 'Pediatr.': [662], '1971;': [663], '78:': [664], '958-967Abstract': [665], 'Scopus': [670, 687, 703, 800, 878, 944, 972, 1002, 1056, 1162, 1183, 1205, 1222, 1260, 1278, 1295, 1368, 1420, 1658, 1735, 1779, 1846, 2110, 2177, 2508, 2551, 2587, 2823, 3276, 3991, 4486], '(147)': [671], '6Melish': [674], 'Turner': [678], 'M.D.': [679], 'Dis.': [682], '1972;': [683], '125:': [684], '129-140Crossref': [685], '(137)': [688], '7Melish': [691], 'N.': [695, 865, 931, 1952, 2353, 2394, 2454, 2665, 2748], 'Engl.': [696], 'Med.': [698, 873, 939], '1970;': [699], '282:': [700], '1114-1119Crossref': [701], '(318)': [704], 'Scholar)': [706, 2403, 2431, 2463, 2511, 4489], 'showed': [707, 3473, 4157], 'injected': [714], 'into': [715, 1701, 1744, 2334, 2438, 2564, 2615, 2701, 3160, 4279, 4300, 4333, 4416], 'mice': [717], 'formation': [720, 3608, 3632, 3662], 'similar': [723, 1074], 'seen': [726], 'SSSS.': [733], 'It': [734], 'remained': [735, 821], 'unclear': [736], 'exactly': [737, 1327], 'how': [738], 'cause': [740], 'until': [743, 824], 'it': [744, 760, 825, 1535, 1541, 4381], 'discovered': [746], 'they': [748, 764, 805, 1023, 1315, 4335], 'structural': [750], 'sequence': [752, 1614, 1680, 3016, 3044, 3058, 3061, 3953], 'homologies': [753], 'proteases,': [756], 'time': [759, 3515, 3526, 3573, 3599], 'suggested': [762], 'act': [765], 'as': [766, 924, 1123, 1629, 1798, 1814, 1930, 1936, 2069, 2569, 2649, 2732, 2791, 2857, 3458, 3595, 3633, 3668], 'proteolytic': [767, 1019], 'enzymes': [768], 'were': [770, 1852, 1876, 2054, 2077, 2332, 2432, 2609, 2699, 2710, 2877, 2887, 2946, 3294, 3307, 3321, 3348, 3364, 3427, 3512, 3538, 3586, 3593, 3609, 3664, 3714, 3733, 3755, 3779, 4038, 4196, 4302, 4441], 'predicted': [771, 1009], 'cleave': [773, 811, 912, 1538, 2999, 3943], 'after': [774, 1331, 4140, 4183], 'glutamic': [777, 1332], 'aspartic': [779], 'acid': [780, 1092, 1333, 1437, 1856, 2850, 3015, 3057, 3118, 3135, 3171, 3952], '(8Vath': [781], 'G.M.': [782], 'Earhart': [783], 'C.A.': [784, 1285], 'Rago': [785], 'J.V.': [786], 'Kim': [787], 'M.H.': [788], 'Bohach': [789], 'G.A.': [790], 'Schlievert': [791], 'P.M.': [792], 'Ohlendorf': [793], 'D.H.': [794], 'Biochemistry.': [795, 1217], '1997;': [796, 1218, 1957, 2101, 2358, 2399, 2459, 2670, 2753], '36:': [797, 1219], '1559-1566Crossref': [798], '(119)': [801], 'However,': [804, 2968], 'could': [806, 4336], 'shown': [809, 2220, 3191, 3533, 3694, 3936], 'model': [812, 1446], 'polypeptide': [813], 'substrates.': [814], 'Their': [815], 'normal': [816], 'pathophysiologic': [817], 'substrate,': [818, 1460, 4152], 'if': [819, 1545, 3842, 4191], 'any,': [820, 3843], 'mystery': [823], 'realized': [827], 'clinical': [837], 'histological': [839], 'similarity': [840], 'those': [842, 2880, 3862, 4444], 'pemphigus': [844], 'foliaceus,': [845], 'an': [846, 1974, 2135, 3868], 'autoimmune': [847], 'pathogenic': [851], 'autoantibodies': [852], 'bind': [853], 'desmosomal': [855, 1064, 1243], 'cell': [856, 1970, 2522], 'adhesion': [857], 'molecule,': [858], '(Dsg1)': [861], '(9Amagai': [862, 928], 'M.': [863, 929, 949, 957, 981, 985, 989, 993, 995, 1044, 1046, 1151, 1153, 1254, 1265, 1356, 1358, 1637, 1641, 1645, 1649, 1651, 1723, 1725, 1767, 1769, 1834, 1836, 1942, 2091, 2095, 2165, 2167, 2343, 2384, 2411, 2444, 2491, 2543, 2575, 2655, 2738, 2811, 2813, 3264, 3266, 4474, 4476], 'Matsuyoshi': [864, 930], 'Wang': [866, 932], 'Z.H.': [867, 933], 'Andl': [868, 934], 'C.': [869, 935, 1032, 1104, 1344, 1403, 1711, 1755, 1822, 2153, 2799, 3252, 3974, 4462], 'Stanley': [870, 936, 958, 1047, 1359, 1406, 1726, 1770, 1837, 2168, 2418, 2814, 3267, 3977, 4477], 'J.R.': [871, 937, 959, 1048, 1360, 1407, 1727, 1771, 1838, 2169, 2419, 2815, 3268, 3978, 4478], 'Nat.': [872, 938, 1286], '2000;': [874, 940, 1291, 2499], '6:': [875, 941], '1275-1277Crossref': [876, 942], '(386)': [879, 945], 'This': [882, 896, 1226, 2181, 3691, 4267], 'observation': [883], 'led': [884], 'hypothesis': [887, 897], 'molecular': [890], 'target': [891], 'validated': [899], 'showing': [901], 'three': [903, 3717], 'different': [904, 4442], 'ET': [905, 1904, 3439, 3673, 3728, 3737, 3764], 'isotypes': [906], '(ETA,': [907], 'ETD)': [910, 3727], 'specifically': [911], 'mouse': [913, 1324, 2408, 2921, 3020, 3047, 4388, 4448], '(hDsg1)': [917], 'closely': [920], 'related': [921], 'such': [923], 'E-cadherin': [927, 2409, 2922, 2969, 2978], '10Amagai': [948], 'Yamaguchi': [950, 1037, 1349, 1716, 1760, 1827, 2158, 2536, 2804, 3257, 4467], 'T.': [951, 977, 987, 1038, 1350, 1399, 1633, 1643, 1717, 1761, 1828, 1946, 1954, 2093, 2159, 2347, 2355, 2388, 2396, 2448, 2456, 2577, 2579, 2659, 2667, 2742, 2750, 2805, 3258, 3970, 4468], 'Hanakawa': [952], 'Y.': [953, 983, 1028, 1040, 1248, 1340, 1352, 1397, 1639, 1707, 1719, 1751, 1763, 1818, 1830, 2149, 2161, 2795, 2807, 3248, 3260, 3968, 4458, 4470], 'Nishifuji': [954, 978, 1041, 1353, 1634, 1720, 1764, 1831, 2162, 2808, 3261, 4471], 'K.': [955, 979, 1042, 1250, 1252, 1354, 1635, 1721, 1765, 1832, 1940, 2163, 2341, 2382, 2442, 2539, 2573, 2653, 2736, 2809, 3262, 4472], 'Sugai': [956, 994, 1043, 1355, 1650, 1722, 1766, 1833, 2164, 2810, 3263, 4473], 'Invest.': [961, 1051, 1363, 1409, 1730, 1774, 1841, 2172, 2421, 2497, 2818, 3271, 3980, 4481], 'Dermatol.': [962, 1410, 2422, 2498, 3981], '2002;': [963, 998, 1052, 1179, 1201, 1364, 1654, 1731, 1775, 1842, 2173, 2819, 3272, 4482], '118:': [964], '845-850Abstract': [965], '(151)': [973], '11Yamaguchi': [976], 'Sasaki': [980, 1636], 'Fudaba': [982, 1039, 1351, 1638, 1718, 1762, 1829, 2160, 2806, 3259, 4469], 'Aepfelbacher': [984, 1640], 'Takata': [986, 1642], 'Ohara': [988, 1644], 'Komatsuzawa': [990, 1646], 'H.': [991, 1036, 1348, 1647, 1715, 1759, 1826, 2097, 2157, 2541, 2803, 3256, 4466], 'Amagai': [992, 1045, 1357, 1648, 1724, 1768, 1835, 1941, 2166, 2342, 2383, 2443, 2574, 2654, 2737, 2812, 3265, 4475], 'Immun.': [997, 1653], '70:': [999, 1655], '5835-5845Crossref': [1000, 1656], '(195)': [1003, 1659], 'Mutation': [1006], 'active': [1010, 1793], 'loss': [1016], 'their': [1018, 2326, 2475, 2627, 4049], 'activity,': [1020], 'confirming': [1021], 'proteases': [1026, 1429, 1502], '(12Hanakawa': [1027, 1339, 1706, 1750, 1817, 2148, 2794, 3247, 4457], 'Schechter': [1029, 1341, 1404, 1708, 1752, 1819, 2150, 2796, 3249, 3975, 4459], 'N.M.': [1030, 1342, 1405, 1709, 1753, 1820, 2089, 2151, 2797, 3250, 3976, 4460], 'Lin': [1031, 1343, 1402, 1710, 1754, 1821, 2152, 2798, 3251, 3973, 4461], 'Garza': [1033, 1345, 1712, 1756, 1823, 2154, 2800, 3253, 4463], 'L.': [1034, 1177, 1346, 1713, 1757, 1824, 2155, 2801, 3254, 4464], 'Li': [1035, 1347, 1714, 1758, 1825, 2156, 2802, 3255, 4465], '110:': [1053, 1365, 1732, 1776, 1843, 2174, 2820, 3273, 4483], '53-60Crossref': [1054, 1366, 1733, 1777, 1844, 2175, 2821, 3274, 4484], '(162)': [1057, 1369, 1736, 1780, 1847, 2178, 2824, 3277, 4487], 'cadherin': [1065, 1069], 'subfamily': [1066], 'supergene': [1070], 'family.': [1071], 'Desmosomal': [1072], 'cadherins,': [1073, 1077, 1137], 'classic': [1076], 'contain': [1078], 'four': [1079, 3416], 'amino-terminal': [1080], 'homologous': [1081, 4384], '(called': [1083], '(EC)': [1085], '1–4),': [1087], 'about': [1089], '100': [1090, 3340, 3551], 'each,': [1093], 'highly': [1096, 4383], 'conserved': [1097, 1125, 3043], 'among': [1098, 1128], 'many': [1099, 1443], 'cadherins': [1100, 1244], '(13Angst': [1101], 'B.D.': [1102], 'Marcozzi': [1103], 'Magee': [1105, 1197], 'A.I.': [1106, 1198], 'Cell': [1108, 1156], 'Sci.': [1109, 2546], '114:': [1111, 2500], '625-626Crossref': [1112], 'A': [1116, 4057], 'fifth': [1117], 'juxtamembrane': [1118], '(EC5)': [1120], 'well': [1124, 3042, 4017], 'within': [1126], 'cadherins.': [1130], 'The': [1131, 1561, 2003, 2063, 2630, 2846, 3031, 3067, 3362, 3380, 3565, 3744, 3797], 'other': [1136, 4025], 'maintained': [1139], 'calcium': [1141], 'occurs': [1144], 'EC': [1146, 1911, 2373, 2558, 2601, 2632, 3915, 4019], '(14Kemler': [1148], 'Ozawa': [1150], 'Ringwald': [1152], 'Curr.': [1154], 'Opin.': [1155], 'Biol.': [1157, 1290, 2099], '1989;': [1158], '1:': [1159, 1292], '892-897Crossref': [1160], '(80)': [1163], '15Boggon': [1166], 'T.J.': [1167], 'Murray': [1168], 'Chappuis-Flament': [1170], 'Wong': [1172], 'E.': [1173, 2480], 'Gumbiner': [1174], 'B.M.': [1175], 'Shapiro': [1176], 'Science.': [1178], '296:': [1180], '1308-1313Crossref': [1181], '(547)': [1184], '16Syed': [1187], 'S.E.': [1188], 'Trinnaman': [1189], 'Martin': [1191], 'Major': [1193], 'Hutchinson': [1195], 'Biochem.': [1199], '362:': [1202], '317-327Crossref': [1203], '(95)': [1206], '17Koch': [1209], 'A.W.': [1210], 'Pokutta': [1211], 'Lustig': [1213], 'A.': [1214, 1246, 1948, 2349, 2390, 2450, 2488, 2661, 2744], 'Engel': [1215, 1266], '7697-7705Crossref': [1220], '(130)': [1223], 'thought': [1230], 'critical': [1233, 4248, 4374], 'maintaining': [1235], 'adhesive': [1237], 'function': [1238, 2071, 3460, 3597], 'classical': [1240, 1801], 'and,': [1241], 'presumably,': [1242], '(18Nagafuchi': [1245], 'Shirayoshi': [1247], 'Okazaki': [1249], 'Yasuda': [1251], 'Takeichi': [1253], 'Nature.': [1255], '1987;': [1256], '329:': [1257], '341-343Crossref': [1258], '(585)': [1261], '19Ozawa': [1264], 'Kemler': [1268], 'Cell.': [1270, 1289], '1990;': [1271], '63:': [1272], '1033-1038Abstract': [1273], '(238)': [1279], '20Green': [1282], 'K.J.': [1283], 'Gaudry': [1284], 'Mol.': [1288], '208-216Crossref': [1293], '(322)': [1296], 'Recent': [1299], 'only': [1303, 1317, 4179, 4289], 'substrate': [1308, 2193, 3566, 3628, 3642], 'also': [1313, 1664, 1784, 3739], 'hydrolyze': [1316, 1442], 'bond': [1320, 1455, 1478], 'same': [1329], 'site,': [1330], '381': [1334], 'EC3': [1336], 'EC4': [1338, 4219], 'Furthermore,': [1372], "former's": [1382], 'conformation': [1384, 2985, 3964, 4326], '(i.e.': [1385, 3681], 'denatured': [1386, 1389], 'partially': [1388], 'cannot': [1391], 'hydrolyzed': [1393, 4340, 4404], 'ETs)': [1395], '(21Hanakawa': [1396, 3967], 'Selwood': [1398, 2092, 3969], 'Woo': [1400, 3971], 'D.': [1401, 3972], '2003;': [1411, 2583, 3982], '121:': [1412, 3983], '383-389Abstract': [1413, 3984], '(44)': [1421, 3992], 'Thus,': [1424], 'unlike': [1425], 'typical': [1427, 1500], 'whose': [1430, 4021], 'specificities': [1431], 'defined': [1433, 1799, 3614, 3676, 4018], 'simple': [1435], 'sequences': [1438, 2961, 3162, 3172, 3181, 3203, 3220, 4255, 4276, 4413], 'proteins': [1444, 3306], 'polypeptides,': [1447], 'correctly': [1458], 'folded': [1459], 'study,': [1464], 'addition': [1469, 3542], 'being': [1471], 'exquisitely': [1472], 'We': [1489, 1783, 4430], 'catalytic': [1493, 1606, 3885], 'protected': [1498], 'inhibitors': [1503, 3831, 3852, 3902], 'amino-terminal,': [1509], 'carboxyl-terminal,': [1512], 'ETA.': [1523, 2967, 3105, 3200, 3838, 4012], 'Finally,': [1524], 'although': [1528, 3867], 'binds': [1530], '(cDsg1)': [1534], 'it,': [1539], 'will': [1542], 'do': [1543], 'so': [1544, 2694], '5': [1546, 3155, 3186, 4345], 'mutated': [1557, 1806, 2142, 2700, 3178, 3202, 3219], 'hDsg1': [1559, 2312, 2376, 2406, 2606, 2702, 2833, 2840, 2870, 2874, 2882, 2885, 2916, 2938, 2941, 2949, 2971, 3008, 3116, 3133, 3454, 3914, 4178, 4257, 4372, 4415, 4446], 'residues.': [1560], 'results': [1562, 3996], 'proper': [1591, 3896, 3912, 4324], 'lock,': [1598], 'properly': [1600], 'align,': [1601], 'perhaps': [1603], 'activate,': [1604], 'site.': [1607, 3054, 3066, 4186], 'Recombinant': [1608, 1850, 2706], 'ETs—DNA': [1609], 'encoding': [1610, 1669, 1685, 2309, 2468, 2592, 3289], 'mature': [1613], 'amplified': [1616], 'PCR': [1618, 1662, 2435, 2612], 'using': [1619, 1982, 2517], 'pQE-ETD-His': [1620], '(provided': [1621], 'Dr.': [1623, 2526], 'Motoyuki': [1624], 'Sugai,': [1625], 'University': [1626, 2529], 'Hiroshima)': [1628], 'template': [1631], '(11Yamaguchi': [1632], 'add': [1667, 1683, 2466, 2620], 'nucleotides': [1668, 1684, 2467], 'signal': [1672], '5′-end': [1676], 'V5': [1687, 1810], 'His': [1689, 1812, 1919, 2472, 2638], 'tags': [1690, 1813, 2318, 2324, 2473, 2625], '3′-end': [1693], 'sequence.': [1697, 3075], 'ETD-V5His': [1698, 1741], 'subcloned': [1700, 2333, 2437, 2563, 2614], 'shuttle': [1703], 'vector,': [1704], 'pCE104': [1705, 1739], 'electroporated': [1743], 'RN4220': [1745], 'electrocompetent': [1748], 'cells': [1749, 2731, 3285], 'recombinant': [1786, 1915, 2330, 2761, 3464], 'wild-type': [1787], 'ETAS195A': [1791, 3297], '(enzymatically': [1792], 'serine,': [1794], 'position': [1796, 2140], '195': [1797, 2141], 'chymotrypsin': [1802], 'numbering,': [1805], 'alanine)': [1808, 2144], 'previously': [1815, 1937, 2122, 2570, 2650, 2733, 2792, 3245], 'described': [1816, 1938, 2571, 2651, 2734, 2793, 3246], 'purified': [1853, 1967, 2013], 'Ni2+-nitrilotriacetic': [1855], 'columns': [1857], '(Qiagen,': [1858], 'Valencia,': [1859], 'CA)': [1860], 'according': [1861, 3766], "manufacturer's": [1864, 2689], 'protocol': [1865, 2690], 'then': [1867, 2043, 2276, 3299, 3333, 3355, 4431], 'dialyzed': [1868, 2006], 'against': [1869, 2007, 3711], 'phosphate-buffered': [1870, 3378, 3423], 'saline.': [1871, 3379], 'Protein': [1872, 1880], 'concentrations': [1873, 2026, 3859], 'estimated': [1877, 2123], 'Assay': [1881], 'Kit': [1882], '(Bio-Rad)': [1883, 3353], 'Simply': [1886, 2049, 3478, 3563], 'Blue': [1887, 2050, 3479], '(a': [1888], 'modified': [1889], 'Coomassie': [1890], 'G-250': [1891], 'solution;': [1892], 'Invitrogen)-stained': [1893], 'SDS-PAGE': [1894, 2046, 3352, 3482, 3521], 'gels': [1895, 2053], 'compared': [1896], 'standards': [1898], 'each': [1900, 4024], 'other.': [1901], 'Production': [1902], 'Cleavage': [1905, 3440, 3925, 4353], 'Human': [1907], 'Desmoglein': [1908], '1—The': [1909], 'entire': [1910, 2631], '(hDsg1E)': [1923, 2194], 'carboxyl': [1926, 2327, 2628, 2645], 'terminus': [1927, 2646], 'secreted': [1932, 2762], 'protein': [1933, 2018, 2763, 3795], 'baculovirus': [1935, 2336, 2565, 2707, 2721, 3288], '(22Ishii': [1939, 2340, 2381, 2441, 2652, 2735], 'Hall': [1943, 2344, 2385, 2445, 2656, 2739], 'R.P.': [1944, 2345, 2386, 2446, 2657, 2740], 'Hashimoto': [1945, 2346, 2387, 2447, 2658, 2741], 'Takayanagi': [1947, 2348, 2389, 2449, 2660, 2743], 'Gamou': [1949, 2350, 2391, 2451, 2662, 2745], 'Shimizu': [1951, 2352, 2393, 2453, 2664, 2747], 'Nishikawa': [1953, 2354, 2395, 2455, 2576, 2666, 2749], 'Immunol.': [1956, 2357, 2398, 2458, 2581, 2669, 2752], '159:': [1958, 2359, 2400, 2460, 2671, 2754], '2010-2017PubMed': [1959, 2360, 2401, 2461, 2672, 2755], 'For': [1962, 2224, 3670], 'kinetic': [1963], 'analysis,': [1964], 'hDsg1E': [1965, 2014, 2116, 2147, 2219, 2282, 2781, 3079, 3487, 3507, 4047, 4237], 'insect': [1969, 3284], 'culture': [1971, 2723, 2768], 'supernatant': [1972, 2769], 'anti-E': [1975, 2788, 2863, 2930, 3092, 3110, 3310, 4105], 'column': [1977], '(Amersham': [1978, 3312, 3388], 'Biosciences)': [1979, 2716, 3313, 3389], 'eluted': [1981, 3334], '0.1': [1983, 2255], 'mg/ml': [1984], '20': [1989, 2243, 3421], 'mm': [1990, 1995, 1998, 2244, 2250], 'Tris,': [1991], 'pH': [1992], '7.4,': [1993], '500': [1994], 'NaCl,': [1996], 'CaCl2': [1999], '(TBS': [2000], 'plus': [2001, 2009, 2037, 2269, 2285, 3330], 'Ca2+).': [2002], 'eluant': [2004], 'TBS': [2008, 2036, 2268, 2284, 3329], 'Ca2+.': [2010, 2270], 'μm': [2012, 2115, 2229, 2233, 2774, 3486], '(estimated': [2015], 'assay': [2019], 'kit': [2020, 2685], '(Bio-Rad))': [2021], 'incubated': [2023, 2277, 2771, 3295, 3365, 3489], 'varying': [2025], 'various': [2033, 2310], 'times': [2034, 3324], 'Ca2+': [2038, 2286, 3331], '25': [2040, 2262, 2288, 3498, 3529], '°C': [2041, 2263, 2289, 3316], 'analyzed': [2044, 2078, 2294], 'stained': [2047, 3561], '(Invitrogen).': [2051], 'scanned': [2055], 'densitometric': [2057], 'analysis': [2058, 3601, 4188, 4286], 'NIH': [2060], 'Image': [2061, 2890, 2989, 3146, 3235], 'software.': [2062], 'percentage': [2064], 'calculated': [2068], 'time,': [2073], 'resultant': [2075], 'curves': [2076, 3613, 3675], 'Igore': [2080], 'Pro': [2081], 'software': [2082], '(WaveMetrics,': [2083], 'Inc.,': [2084], 'Lake': [2085], 'Oswego,': [2086], 'OR)': [2087], '(23Schechter': [2088], 'Plotnick': [2090], 'Walter': [2094], 'Rubin': [2096], 'Chem.': [2100], '272:': [2102], '24499-24507Abstract': [2103], '(22)': [2111], 'chosen': [2118], 'because': [2119, 4113, 4330, 4380], 'dissociation': [2124], 'constant': [2125, 3626], '(Kd)': [2126], '∼10': [2128], 'μm,': [2129], 'determined': [2130, 3645, 4032, 4432], 'interaction': [2133], '(active': [2137], 'value': [2182], 'suggests': [2183], 'lower': [2185, 3578], 'limit': [2186], 'Km,': [2189, 2199], 'concentration': [2195, 3643, 3651, 3847], '∼10-fold': [2196, 3869], 'Michaelis-Menten': [2201], 'equation': [2202], 'predicts': [2203], 'pseudo-first-order': [2204, 3742], 'kinetics': [2205, 3680], 'any': [2207], 'particular': [2208, 4364], 'concentration,': [2210, 3674], 'confirmed': [2213, 4270], 'exponential': [2216, 3612], 'decay': [2217], 'Fig.': [2222, 3535, 3700], '1.': [2223, 2858], 'inhibitor': [2226], 'studies,': [2227], 'staphylococcus': [2234], 'V8': [2235, 2274, 3856], '(Roche': [2237, 2258], 'Applied': [2238, 2259], 'Science)': [2239, 2260], 'preincubated': [2241], '(DFP)': [2246], '(Calbiochem)': [2247], '2': [2249, 3619], '(DCI)': [2252], '(Sigma)': [2253], 'units/μl': [2256], 'α2-macroglobulin': [2257], '30': [2265], 'min': [2266], 'Pretreated': [2271], '10': [2279, 2759, 3323], 'nm': [2280, 2760, 3495, 3501, 3530], 'h': [2292, 3368, 3411], 'Western': [2296, 2785, 2860, 2927, 3094, 3107, 3342, 4108], 'blotting.': [2297], 'Truncated': [2299], 'Mutants': [2300], 'Chimeric': [2302], 'Recombinants—PCR': [2303], 'construct': [2307, 3193, 4199], 'cDNAs': [2308, 2591], '(Fig.': [2314, 2607, 2704, 3483, 3602, 3720, 3865, 4055, 4090, 4110, 4164, 4249, 4281, 4343, 4399, 4407, 4425, 4490], '3A)': [2315], 'tag-glutathione': [2321], '(GST)': [2323], 'termini.': [2328, 2629], 'constructs': [2331], 'expression': [2337, 2566, 2708], 'vector': [2338, 2567], 'pEVmod': [2339], 'Various': [2363], 'domain-swapped': [2364, 2596, 2903, 2913], '(Figs.': [2367], '4A': [2368], '5A)': [2370], '(hDsg3)': [2380], '(24Amagai': [2410], 'Karpati': [2412], 'Klaus-Kovtun': [2414], 'V.': [2415, 2492], 'Udey': [2416], 'M.C.': [2417], '1994;': [2423], '102:': [2424], '402-408Abstract': [2425], 'pEV-EHis': [2440, 2568], 'cassette': [2464, 2618], '3′-ends.': [2476], 'cDsg1': [2477, 2561, 2594, 2604, 2678, 3010, 3023, 3050, 3072, 3122, 3139, 3159, 3175, 4379, 4417, 4440], 'cDNA': [2478, 2555], '(25Muller': [2479], 'Caldelari': [2481], 'Levine': [2483], 'Kaplan': [2485], 'Baron': [2487], 'Wyder': [2489], 'RohrbachB': [2490], 'Balmer': [2493], 'M.M.': [2494], 'Suter': [2495], '1211-1213Abstract': [2501], '(13)': [2509], 'cloned': [2513], 'reverse': [2515], 'transcriptase-PCR': [2516], 'RNA': [2518], 'line': [2523], 'MCA-B1': [2524], '(from': [2525], 'Susumu': [2527], 'Tateyama;': [2528], 'Miyazaki)': [2531], '(26Priosoeryanto': [2532], 'B.P.': [2533], 'Tateyama': [2534], 'Uchida': [2538], 'Ogawa': [2540], 'Nakai': [2542], 'Res.': [2544], 'Vet.': [2545, 2580], '1995;': [2547], '58:': [2548], '101-102Crossref': [2549], '(9)': [2552], '(27Nishifuji': [2572], 'Iwasaki': [2578], 'Immunopathol.': [2582], '95:': [2584], '177-182Crossref': [2585], '(15)': [2588], '6C)': [2608], 'pEV-E-GST': [2617], 'GST': [2624, 4136, 4173], 'hDsg3': [2635, 2843, 2918, 2934, 4239], '(hDsg3E)': [2642], 'Site-directed': [2675], 'mutagenesis': [2676, 2684], 'performed': [2680], 'Genetailor': [2683], '(Invitrogen)': [2686], 'following': [2687], 'make': [2692], 'selected': [2696], 'equivalents': [2703], '7A).': [2705], 'vectors': [2709], 'co-transfected': [2711], 'BaculoGold': [2713], 'DNA': [2714], '(BD': [2715], 'Sf9': [2718], 'cells,': [2719], 'supernatants': [2724, 3280], 'infect': [2728], 'High': [2729, 2766, 3282], 'Five': [2730, 2767, 3283], 'Approximately,': [2758], 'analysis.': [2778], 'Degradation': [2779], 'assayed': [2783], 'blotting': [2786, 2928, 3095, 3108, 4109], 'antibodies,': [2790], 'Scholar).Fig.': [2826], '4ETA': [2827], 'hDsg3.A,': [2835], 'schematic': [2836, 2910], 'diagram': [2837, 2911], '(white)': [2841], '(gray)': [2844], 'constructs.': [2845], 'numbers': [2847], 'indicate': [2848, 3789], 'counting': [2852], 'initiating': [2855], 'methionine': [2856], 'B,': [2859, 2926, 3055, 3183], 'blot': [2861], 'indicates': [2865, 3033, 4189], 'containing': [2869, 2881, 2970], '1–450': [2872], '213–545': [2876], 'cleaved,': [2878, 2947, 2975], '312–545': [2883], '1–309': [2886], 'not.View': [2888], 'Large': [2889, 2988, 3145, 3234], 'Figure': [2891, 2990, 3147, 3236], 'ViewerDownload': [2892, 2991, 3148, 3237], 'Hi-res': [2893, 2992, 3149, 3238], 'image': [2894, 2993, 3150, 3239], 'Download': [2895, 2994, 3151, 3240], '(PPT)Fig.': [2896, 2995, 3152], '5Susceptibility': [2897], 'hDsg1,': [2906, 3019, 3046, 4452], 'hDsg3,': [2907], 'E-cadherin.A,': [2909], '(white),': [2917, 3080], '(gray),': [2919, 3082], '(E-cad)': [2923], '(dotted': [2924], 'box).': [2925], 'shows': [2932, 3096, 3112], 'substitution': [2936, 3002, 3114, 3131], '214–450': [2939, 2972], '214–398': [2942, 4292], '(data': [2943, 3142, 3877, 4306], 'shown)': [2945], 'whereas': [2948, 3849], '292–450': [2950], 'not,': [2952], 'junction': [2956, 3006, 4421, 4438], 'EC2-EC3': [2958, 3005, 4295, 4369, 4420], 'contains': [2959], 'susceptibility': [2963, 3011, 3125, 3165, 3196, 4050], 'provide': [2981], 'overall': [2984, 4230, 4313, 4325], 'cleavage.View': [2987], '6ETA': [2996], 'cDsg1,': [3000, 3190], 'restores': [3009, 3163, 3195], 'cleavage.A,': [3013], 'alignment': [3017, 3059, 3897], 'around': [3024, 3051, 4395], 'toxins.': [3030], 'arrow': [3032], 'ETs.': [3038], 'Note': [3039, 3216], 'underlined': [3068], 'nonidentical': [3074], 'C,': [3076, 3201], 'comparison': [3077], 'cDsg1E': [3081], 'cDsg1EC1–3EGST,': [3084], 'cDsg1EC1–3EGST': [3086], 'substituted': [3087], 'sequences.': [3090], 'D,': [3091], 'cDsg1E,': [3098, 3291], 'hDsg3E,': [3100], 'cleaved': [3103, 3571, 4454], 'E,': [3106], '214–334': [3120], 'restored': [3123], '214–257': [3137], 'did': [3140, 3853, 3874], 'shown).View': [3144], '7Mutation': [3153], 'ETA.A,': [3169], 'point-mutated': [3174], 'underlined.': [3182], 'mutation': [3184], 'c271–7,': [3194], '(in': [3204, 3230], 'black,': [3205, 3231], 'thin': [3206], 'arrows)': [3207], 'c271–7': [3209], 'superimposed': [3210], 'c-cadherin': [3213], 'crystal': [3214], 'structure.': [3215], 'loop': [3224], 'proximal': [3225], 'thick': [3232], 'arrow).View': [3233], '(PPT)': [3241], 'Immunoprecipitation—As': [3242], 'methods': [3244], 'Scholar),': [3279], 'transduced': [3286], 'hDsg1E,': [3290], 'hDsg3E': [3293], 'directly': [3301, 3734], 'immunoprecipitation.': [3303], 'tagged': [3305], 'precipitated': [3308], 'tag-Sepharose': [3311], 'h.': [3319], 'Immunoprecipitates': [3320], 'washed': [3322], '1%': [3326], 'Triton': [3327], 'X-100': [3328], 'Laemmli': [3336, 3345], 'sample': [3337, 3346], 'buffer': [3338, 3347, 3371, 3546], '°C.': [3341], 'Blotting—Proteins': [3343], 'separated': [3349], '4–20%': [3351, 3556], 'transferred': [3356], 'nitrocellulose': [3358], 'sheets': [3359, 3363], '(Transblot;': [3360], 'Bio-Rad).': [3361], 'blocking': [3370, 3405], '5%': [3373], 'fat-free': [3374], 'milk': [3375], 'powder': [3376], 'antibody': [3383, 3394], 'conjugated': [3384, 3395], 'horseradish': [3386, 3397], 'peroxidase': [3387, 3398], 'anti-ETA': [3391], 'sheep': [3392], 'polyclonal': [3393], '(Toxin': [3399], 'Technology,': [3400], 'Sarasota,': [3401], 'FL),': [3402], 'diluted': [3403], 'buffer,': [3406], 'applied': [3408], 'room': [3413], 'temperature.': [3414], 'After': [3415, 4092], 'washes': [3417], '0.1%': [3419], 'Tween': [3420], 'saline,': [3424], 'signals': [3426], 'detected': [3428, 4103], 'chemiluminescence': [3430], '(ECL': [3431], 'ECL': [3433], 'Plus;': [3434], 'Amersham': [3435], 'Biosciences).': [3436], 'Kinetics': [3437], 'hDsg1—To': [3442], 'investigate': [3443], 'hydrolysis': [3447, 4005, 4052], 'ETs,': [3451], 'measured': [3453], 'time.': [3462, 3712], 'Purified': [3463], 'experiments': [3472], 'band': [3476], 'staining': [3480], '1A).': [3484], '12.5,': [3491], '25,': [3492], '50': [3494, 3500], 'ETB': [3503, 3725], 'ETD.': [3505], 'Both': [3506], 'disappearance': [3508, 3660], 'product': [3510, 3607, 3631, 4161], 'appearance': [3511], 'quantified': [3513, 3587], 'over': [3514], 'bands': [3517, 3585], 'resolved': [3518, 3553], 'SDS-PAGE.': [3520], 'representative': [3524], 'course': [3527], '1B.': [3536], 'Reactions': [3537], 'stopped': [3539], 'SDS': [3544], 'denaturing': [3545], 'subsequent': [3548], 'heating': [3549], '°C,': [3552], 'gradient': [3557], 'polyacrylamide': [3558], 'gel,': [3559], 'Blue.': [3564], '(upper': [3567], 'band,': [3568], 'arrow)': [3569], 'products': [3576], '(two': [3577], 'bands,': [3579], 'arrowhead': [3580], 'open': [3582], 'arrow).': [3583], 'Stained': [3584], 'densitometry,': [3589], 'plotted': [3594, 3710], '1C).': [3603], 'Substrate': [3604], 'consumption': [3605, 3629], 'fit': [3610, 3686, 3698], 'Equations': [3616, 3682], 'obtain': [3621], 'kobs,': [3622], 'observed': [3624], 'rate': [3625, 3656], 'follows,[S]=[S]0e-kobst(Eq.': [3634], '1)': [3635], '[P]=[S]0-[S]0e-kobst(Eq.': [3636], '2)': [3637, 3685], 'where': [3638], '[S]': [3639, 3708], 'represents': [3640, 3650], '(as': [3644], 'densitometry)': [3647], '[P]': [3649], 'products.': [3654], 'Observed': [3655], 'constants': [3657], 'obtained': [3658, 3756, 3780], 'good': [3666], 'agreement,': [3667], 'expected.': [3669], 'given': [3672], 'first': [3678, 4031], 'order': [3679], 'points': [3689], 'well.': [3690], 'best': [3693], 'linear': [3697], '1D,': [3701], 'natural': [3705], 'log': [3706], 'Measurements': [3713], 'made': [3715], '(for': [3718, 3724], 'ETA)': [3719], '1D)': [3721], 'two': [3723], 'concentrations.': [3729], 'Values': [3730, 3771], 'kobs': [3732], 'proportional': [3735], 'concentrations,': [3738, 3765], 'consistent': [3740], 'reactions.': [3743], 'kcat/Km,': [3747], 'measure': [3751], 'efficiency,': [3754], 'slopes': [3759], 'plots': [3761], 'kobsversus': [3763], 'Equation': [3768], '3,kcatKm=kobs[E](Eq.': [3769], '3)': [3770], '62,000,': [3773], '26,000,': [3774], '19,000': [3776], 'm–1': [3777], 's–1': [3778], 'ETD,': [3785], 'respectively.': [3786], 'large': [3794, 3825], 'Broad': [3798], 'Spectrum': [3799], 'Serine': [3800], 'Protease': [3801], 'Inhibitors': [3802], 'DFP,': [3803], 'DCI,': [3804], 'α2-Macroglobulin': [3806], 'Do': [3807], 'Not': [3808, 3921], 'Effectively': [3809], 'Inhibit': [3810], 'Enzymatic': [3812], 'Activity': [3813], 'ETA—We': [3815], 'tested': [3816], 'ability': [3818, 3939], 'small': [3820, 4123], '(DFP': [3821], 'DCI)': [3823], '(α2-macroglobulin)': [3826], 'inhibit': [3833, 3855], 'enzymatic': [3835, 4009], 'There': [3839], 'minimal,': [3841], 'inhibition': [3844, 3876], 'tested,': [3848], 'both': [3850], 'equivalent': [3860], '2),': [3866], 'larger': [3870], 'excess': [3871], 'DCI': [3873], 'shown).': [3879, 4308], 'difficult': [3891], 'access': [3893], 'some': [3899], 'relatively': [3905], 'absence': [3909], 'Domains': [3916], 'That': [3917], 'Are': [3918, 3927, 4357], 'Upstream,': [3919], 'Downstream,': [3922], 'Site': [3926, 4354], 'Critical': [3928], 'Its': [3930], 'Hydrolysis': [3931], 'ETA—Previously,': [3933], 'simply': [3947], 'distant': [4000], 'might': [4006], 'influence': [4007], 'Because': [4013, 4222], 'desmogleins': [4014], 'five': [4016], 'relationship': [4022], 'stabilized': [4027], 'calcium,': [4029], 'whether': [4033], 'all': [4034], 'testing': [4043], 'B).': [4059], 'indicated': [4062, 4287], 'EC5': [4064], 'Loss': [4070, 4085], 'EC1': [4072], 'decreases': [4073], 'yet': [4078, 4401], 'molecule': [4081], 'still': [4083], 'cleaved.': [4084], 'EC1-EC2': [4087], 'prevents': [4088], '3B).': [4091], 'incubation': [4093], 'EC1–EC3': [4097], 'no': [4100, 4201, 4337], 'longer': [4101, 4202, 4338], '3B),': [4111], 'presumably': [4112], 'tag,': [4119], 'too': [4122], 'detect': [4125], 'gels.': [4128], 'To': [4129], 'confirm': [4130], 'EC1–EC3,': [4134], 'added': [4139, 4176], 'carboxyl-terminal': [4146], 'end.': [4147], 'Incubation': [4148], 'new': [4151], 'EC1–3EGST,': [4153], 'clearly': [4156], 'now': [4162], 'detectable': [4163], '3C).': [4165], 'construct,': [4168], 'combination': [4170], 'Further': [4187], 'truncated,': [4197], 'susceptible': [4203], 'intact': [4217], 'EC5.': [4221], 'truncations': [4223], 'result': [4225], 'generally': [4228], 'unstable': [4229], 'structure,': [4231], 'swapping': [4235], 'test': [4242], '4).': [4250], '213': [4259], '450': [4261], 'probably': [4263], 'sufficient': [4264, 4303, 4310], 'conclusion': [4268], 'insertion': [4272], 'just': [4274], '5).': [4282], 'fact,': [4284], 'finer': [4285], 'inserted': [4299, 4332], 'Dsg3,': [4301, 4316], 'must': [4320], 'desmoglein,': [4329], 'when': [4331], 'E-cadherin,': [4334], '5B).': [4344], 'Amino': [4346], 'Acids': [4347], '110': [4348], 'Residues': [4349], 'Upstream': [4350], 'Necessary': [4358], 'Cleavage—To': [4360], 'region': [4370], 'shares': [4391], 'identical': [4392], '6A)': [4400], '6,': [4408, 4426], 'C': [4409, 4427], 'D).': [4411], 'Swapping': [4412], 'near': [4418], 'allowed': [4422], 'E).': [4429], 'which,': [4450], '6B).': [4491], 'Be': [4492]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2013693602', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-14T03:49:29.064940', 'created_date': '2016-06-24'}
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