Title: Involvement of 3,4-dichlorophenol hydroxylase in degradation of 3,4-dichlorophenol by the white rot fungus Phanerochaete chrysosporium
Abstract: Abstract Phanerochaete chrysosporium was able to grow in a C-limited medium containing 3,4-dichlorophenol. In comparison with cultures without 3,4-dichlorophenol, we observed about 30% reduction of the biomass together with the lack of lignin peroxidase and manganese peroxidase production. A weak dechlorination was observed but only methylated dichloroaromatic products such as 3,4-dichloroanisole, 4,5-dichloroguaiacol, and 4,5-dichloroveratrole were identified as chlorinated metabolites showing that methylation, but also hydroxylation reactions occurred. To confirm this hydroxylation reaction we have studied the degradation of 3,4-dichlorophenol, and of several chlorinated metabolites by mycelial pellets of P. chrysosporium lacking peroxidase activity. Under these conditions the degradation and the dechlorination of 3,4-dichlorophenol increased strongly and the amount of chloride recovered was 80% of the stoichiometric amount recoverable. We observed similar results with 4,5-dichlorocatechol. An NADPH-dependent 3,4-dichlorophenol hydroxylase was detected in cell free extracts and 4,5-dichlorocatechol was identified as hydroxylation product.
Publication Year: 1998
Publication Date: 1998-09-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 9
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