Title: Next generation sequencing applications in agro biotechnology
Abstract: The principle of next generation sequencing (NGS) is a massively multiparallel sequencing of DNA fragments. Existing NGS platforms differ significantly. The second generation instruments require clonal amplification of DNA molecules; the third generation technology enables sequencing at the single-molecule level. The prototypic instruments from the former category are the GS FLX+ (454, Roche), SOLiD and Ion PGM (Life Technologies), and HiSeq (Illumina), whereas instruments from Helicos and Pacific Biosciences represent the latter category. NGS platforms differ with regard to throughput, read length, and quality; number of reads in a single run; speed of sequencing; and paired versus single read approach. In parallel with a steady increase in throughput, a trend toward the development of small-scale machines for clinical use ("benchtop" sequencers) has opened prospects for NGS implementation in clinics. Benchtop sequencers usually require preselection (enrichment) of targets achieved by PCR and/or hybridization. Design and standardization of enrichment techniques are important aspects of NGS clinical use.
Publication Year: 2012
Publication Date: 2012-09-25
Language: en
Type: article
Indexed In: ['crossref']
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